VPS34

Summary

Gene Symbol: VPS34
Description: phosphatidylinositol 3-kinase VPS34
Alias: END12, PEP15, STT8, VPL7, VPS7, VPT29, phosphatidylinositol 3-kinase VPS34
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Munn A, Riezman H. Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol. 1994;127:373-86 pubmed
    ..end8-1, end9-1, end10-1, and end11-1) show altered uptake of the endocytosed ligand, alpha-factor, and three (end12-1, end12-2, and end13-1) are probably defective in transfer of internalized material to the vacuole...
  2. Schu P, Takegawa K, Fry M, Stack J, Waterfield M, Emr S. Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Science. 1993;260:88-91 pubmed
    The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae...
  3. Obara K, Sekito T, Niimi K, Ohsumi Y. The Atg18-Atg2 complex is recruited to autophagic membranes via phosphatidylinositol 3-phosphate and exerts an essential function. J Biol Chem. 2008;283:23972-80 pubmed publisher
    ..Taken together, Atg18 forms a complex with Atg2 irrespective of PtdIns(3)P binding, associates tightly to autophagic membranes by interacting with PtdIns(3)P, and plays an essential role. ..
  4. Tall G, Hama H, DeWald D, Horazdovsky B. The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting. Mol Biol Cell. 1999;10:1873-89 pubmed
    ..Vac1p FYVE finger mutant missorting phenotypes were suppressed by a defective allele of VPS34. These data indicate that PtdIns(3)P may perform a regulatory role, possibly involved in mediating Vac1p protein-..
  5. Obara K, Sekito T, Ohsumi Y. Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae. Mol Biol Cell. 2006;17:1527-39 pubmed
    ..Together, these results indicate that complexes I and II function in distinct biological processes by localizing to specific compartments in a manner mediated by specific components of each complex, Atg14p and Vps38p, respectively. ..
  6. Kihara A, Noda T, Ishihara N, Ohsumi Y. Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J Cell Biol. 2001;152:519-30 pubmed
    ..These results indicate that Vps30p functions as a subunit of a Vps34 PtdIns 3-kinase complex(es)...
  7. Oliver M, Fernández Acero T, Luna S, Rodríguez Escudero I, Molina M, Pulido R, et al. Insights into the pathological mechanisms of p85α mutations using a yeast-based phosphatidylinositol 3-kinase model. Biosci Rep. 2017;37: pubmed publisher
  8. Stack J, Herman P, Schu P, Emr S. A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole. EMBO J. 1993;12:2195-204 pubmed
    The Vps15 protein kinase and the Vps34 phosphatidylinositol 3-kinase (PI 3-kinase) are required for the sorting of soluble hydrolases to the yeast vacuole...
  9. Slessareva J, Routt S, Temple B, Bankaitis V, Dohlman H. Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome. Cell. 2006;126:191-203 pubmed
    ..Our analysis reveals a requirement for both the catalytic (Vps34) and regulatory (Vps15) subunits of the sole phosphatidylinositol 3-kinase in yeast...

More Information

Publications23

  1. Gaur N, Hasek J, Brickner D, Qiu H, Zhang F, Wong C, et al. Vps factors are required for efficient transcription elongation in budding yeast. Genetics. 2013;193:829-51 pubmed publisher
    ..We found that yeast mutants lacking the phosphatidylinositol 3-phosphate [PI(3)P] kinase Vps34 or its associated protein kinase Vps15 display multiple phenotypes indicating impaired transcription elongation...
  2. Zurita Martinez S, Puria R, Pan X, Boeke J, Cardenas M. Efficient Tor signaling requires a functional class C Vps protein complex in Saccharomyces cerevisiae. Genetics. 2007;176:2139-50 pubmed
  3. Wiederhold E, Gandhi T, Permentier H, Breitling R, Poolman B, Slotboom D. The yeast vacuolar membrane proteome. Mol Cell Proteomics. 2009;8:380-92 pubmed publisher
    ..Our work provides a wealth of information on vacuolar biology and a solid basis for further characterization of vacuolar functions. ..
  4. Grunau S, Lay D, Mindthoff S, Platta H, Girzalsky W, Just W, et al. The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae. Biochem J. 2011;434:161-70 pubmed publisher
    ..However, although Vps34 is required, it is not essential for optimal peroxisome biogenesis...
  5. Tahirovic S, Schorr M, Mayinger P. Regulation of intracellular phosphatidylinositol-4-phosphate by the Sac1 lipid phosphatase. Traffic. 2005;6:116-30 pubmed
    ..We conclude that Sac1p functions in confining PtdIns(4)P-dependent processes to specific intracellular membranes. ..
  6. Obara K, Noda T, Niimi K, Ohsumi Y. Transport of phosphatidylinositol 3-phosphate into the vacuole via autophagic membranes in Saccharomyces cerevisiae. Genes Cells. 2008;13:537-47 pubmed publisher
    b>Vps34, the sole PtdIns 3-kinase in yeast, is essential for autophagy. Here, we show that the lipid-kinase activity of Vps34 is required for autophagy, implying an essential role of its product PtdIns(3)P...
  7. Gurunathan S, David D, Gerst J. Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast. EMBO J. 2002;21:602-14 pubmed
    ..e. VPS34 and VAM3, respectively) have no effect...
  8. Herman P, Stack J, Emr S. An essential role for a protein and lipid kinase complex in secretory protein sorting. Trends Cell Biol. 1992;2:363-8 pubmed
    ..Recent data on two of these genes, VPS15 and VPS34, are beginning to provide some fundamental insights into the mechanisms governing protein sorting within the ..
  9. LeBlanc M, McMaster C. Lipid binding requirements for oxysterol-binding protein Kes1 inhibition of autophagy and endosome-trans-Golgi trafficking pathways. J Biol Chem. 2010;285:33875-84 pubmed publisher
    ..regulation of the autophagy/cytoplasm to the vacuole trafficking pathway was prevented by increasing expression of the PI3K Vps34, suggesting that it is the Kes1-mediated decrease in PI3P level that contributes to this regulation.
  10. Stack J, Emr S. Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem. 1994;269:31552-62 pubmed
    The Vps15 protein kinase and the Vps34 phosphatidylinositol 3-kinase have been shown to function as a membrane-associated complex which facilitates the delivery of proteins to the vacuole in yeast...
  11. Budovskaya Y, Hama H, DeWald D, Herman P. The C terminus of the Vps34p phosphoinositide 3-kinase is necessary and sufficient for the interaction with the Vps15p protein kinase. J Biol Chem. 2002;277:287-94 pubmed
    ..This study thus represents a first step toward a better understanding of how this Vps15p.Vps34p kinase complex is assembled and regulated in vivo. ..
  12. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..The Vps52/53/54 complex joins a growing list of distinct multisubunit complexes that regulate membrane-trafficking events. ..
  13. Alibhoy A, Giardina B, Dunton D, Chiang H. Vps34p is required for the decline of extracellular fructose-1,6-bisphosphatase in the vacuole import and degradation pathway. J Biol Chem. 2012;287:33080-93 pubmed
    ..Moreover, the reduction of extracellular FBPase was also dependent on the VPS34 gene. VPS34 encodes the PI3 kinase and is also required for the Vid pathway...
  14. Araki Y, Ku W, Akioka M, May A, Hayashi Y, Arisaka F, et al. Atg38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity. J Cell Biol. 2013;203:299-313 pubmed publisher
    ..Autophagy is characterized by the formation of an autophagosome, for which Vps34-dervied phosphatidylinositol 3-phosphate (PI3P) is essential...