VPS3

Summary

Gene Symbol: VPS3
Description: CORVET complex subunit VPS3
Alias: PEP6, VPL3, VPT17, CORVET complex subunit VPS3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Raymond C, O Hara P, Eichinger G, Rothman J, Stevens T. Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle. J Cell Biol. 1990;111:877-92 pubmed
    b>vps3 mutants of the yeast Saccharomyces cerevisiae are impaired in the sorting of newly synthesized soluble vacuolar proteins and in the acidification of the vacuole (Rothman, J. H., and T. H. Stevens. Cell. 47:1041-1051; Rothman, J. H...
  2. Cabrera M, Arlt H, Epp N, Lachmann J, Griffith J, Perz A, et al. Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem. 2013;288:5166-75 pubmed publisher
    ..including the Vac1 tether and the Class C core vacuole/endosome tethering (CORVET) complex-specific subunits Vps3 and Vps8, results in a class D vps phenotype...
  3. Markgraf D, Ahnert F, Arlt H, Mari M, Peplowska K, Epp N, et al. The CORVET subunit Vps8 cooperates with the Rab5 homolog Vps21 to induce clustering of late endosomal compartments. Mol Biol Cell. 2009;20:5276-89 pubmed publisher
    ..This clustering, however, requires Vps3, Vps16, and Vps33 but not the remaining CORVET subunits...
  4. Plemel R, Lobingier B, Brett C, Angers C, Nickerson D, Paulsel A, et al. Subunit organization and Rab interactions of Vps-C protein complexes that control endolysosomal membrane traffic. Mol Biol Cell. 2011;22:1353-63 pubmed publisher
    ..Functional modules coordinate the major Rab interactions within CORVET and HOPS. The CORVET-specific subunits, Vps3 and Vps8, form a subcomplex and physically and genetically interact with the Rab5 orthologue Vps21...
  5. Ostrowicz C, Bröcker C, Ahnert F, Nordmann M, Lachmann J, Peplowska K, et al. Defined subunit arrangement and rab interactions are required for functionality of the HOPS tethering complex. Traffic. 2010;11:1334-46 pubmed publisher
    ..and CORVET, the class C proteins Vps11 and Vps18 connect the two parts, and Vps11 binds both HOPS Vps39 and CORVET Vps3 via the same binding site...
  6. Raymond C, Howald Stevenson I, Vater C, Stevens T. Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell. 1992;3:1389-402 pubmed
    ..A good correlation between morphological phenotype and the extent of the CPY sorting defect was observed. ..
  7. Peplowska K, Markgraf D, Ostrowicz C, Bange G, Ungermann C. The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesis. Dev Cell. 2007;12:739-50 pubmed
    ..contains Vps41/Vam2 and Vam6, whereas the CORVET complex has the Vps41 homolog Vps8 and the (h)Vam6 homolog Vps3. Strikingly, the CORVET and HOPS complexes can interconvert; we identify two additional intermediate complexes, ..
  8. Rutledge R, Ghislain M, Mullins J, de Thozée C, Golin J. Pdr5-mediated multidrug resistance requires the CPY-vacuolar sorting protein Vps3: are xenobiotic compounds routed from the vacuole to plasma membrane transporters for efflux?. Mol Genet Genomics. 2008;279:573-83 pubmed publisher
    ..The vacuole also plays a critical role in multidrug resistance. Mutations in genes such as VPS3 that are essential for vacuolar acidification and carboxypeptidase Y vacuolar protein-sorting are multidrug ..
  9. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..The Vps52/53/54 complex joins a growing list of distinct multisubunit complexes that regulate membrane-trafficking events. ..

More Information

Publications17

  1. Conchon S, Cao X, Barlowe C, Pelham H. Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex. EMBO J. 1999;18:3934-46 pubmed
    ..and found one that affects a previously uncharacterized membrane protein, Got1p, as well as new alleles of sed5 and vps3. Got1p is an evolutionarily conserved non-essential protein with a membrane topology similar to that of Sft2p...
  2. Lo S, Brett C, Plemel R, Vignali M, Fields S, Gonen T, et al. Intrinsic tethering activity of endosomal Rab proteins. Nat Struct Mol Biol. 2011;19:40-7 pubmed publisher
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion. ..
  3. Balderhaar H, Lachmann J, Yavavli E, Bröcker C, Lürick A, Ungermann C. The CORVET complex promotes tethering and fusion of Rab5/Vps21-positive membranes. Proc Natl Acad Sci U S A. 2013;110:3823-8 pubmed publisher
    ..We therefore conclude that CORVET is a tethering complex that promotes fusion of Rab5-positive membranes and thus facilitates receptor down-regulation and recycling at the late endosome. ..
  4. Epp N, Ungermann C. The N-terminal domains of Vps3 and Vps8 are critical for localization and function of the CORVET tethering complex on endosomes. PLoS ONE. 2013;8:e67307 pubmed publisher
    ..Here, we show that the CORVET-specific Vps3 and Vps8 subunits, which interact with Rab5/Vps21, require their N-terminal domains for localization and function...
  5. Kingsbury J, Sen N, Maeda T, Heitman J, Cardenas M. Endolysosomal membrane trafficking complexes drive nutrient-dependent TORC1 signaling to control cell growth in Saccharomyces cerevisiae. Genetics. 2014;196:1077-89 pubmed publisher
  6. Fayyadkazan M, Tate J, Vierendeels F, Cooper T, Dubois E, Georis I. Components of Golgi-to-vacuole trafficking are required for nitrogen- and TORC1-responsive regulation of the yeast GATA factors. Microbiologyopen. 2014;3:271-87 pubmed publisher
    ..As a result, the current model describing participation of Vps system components in events associated with translocation of Gln3 into the nucleus following rapamycin treatment or growth in nitrogen-poor medium requires modification. ..
  7. Díaz Blanco N, RODRIGUEZ MEDINA J. Dosage rescue by UBC4 restores cell wall integrity in Saccharomyces cerevisiae lacking the myosin type II gene MYO1. Yeast. 2007;24:343-55 pubmed
    ..These results point to Ubc4p as an important enzyme in the process of cell wall remodelling in myo1 cells. ..
  8. Stepp J, Huang K, Lemmon S. The yeast adaptor protein complex, AP-3, is essential for the efficient delivery of alkaline phosphatase by the alternate pathway to the vacuole. J Cell Biol. 1997;139:1761-74 pubmed
    ..We suggest that some AP-3-dependent cargo proteins that regulate late steps in Golgi to vacuole transport are diverted into the Vps pathway allowing completion of transfer to the vacuole in the class E vps mutant. ..