VPS27

Summary

Gene Symbol: VPS27
Description: ESCRT-0 subunit protein VPS27
Alias: DID7, GRD11, SSV17, VPL23, VPT27, ESCRT-0 subunit protein VPS27
Species: Saccharomyces cerevisiae S288c
Products:     VPS27

Top Publications

  1. Fisher R, Wang B, Alam S, Higginson D, Robinson H, Sundquist W, et al. Structure and ubiquitin binding of the ubiquitin-interacting motif. J Biol Chem. 2003;278:28976-84 pubmed
    ..Although Vps27p-2 is monomeric in solution, the motif unexpectedly crystallized as an antiparallel four-helix bundle, and the potential biological implications of UIM oligomerization are therefore discussed. ..
  2. Nikko E, Andre B. Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase. Eukaryot Cell. 2007;6:1266-77 pubmed
    ..Our data indicate that Bro1 interacts with several ESCRT components and contributes via its PRD to associating ubiquitinating and deubiquitinating enzymes with the MVB sorting machinery. ..
  3. Ren J, Pashkova N, Winistorfer S, Piper R. DOA1/UFD3 plays a role in sorting ubiquitinated membrane proteins into multivesicular bodies. J Biol Chem. 2008;283:21599-611 pubmed publisher
    ..The Vps27-Hse1 complex, which is homologous to the Hrs-STAM complex in mammalian cells, serves as a Ub-sorting receptor at ..
  4. Nothwehr S, Bryant N, Stevens T. The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol. 1996;16:2700-7 pubmed
    ..All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its ..
  5. Scott P, Bilodeau P, Zhdankina O, Winistorfer S, Hauglund M, Allaman M, et al. GGA proteins bind ubiquitin to facilitate sorting at the trans-Golgi network. Nat Cell Biol. 2004;6:252-9 pubmed
    ..Thus, GGA proteins fulfill the role of ubiquitin sorting receptors at the TGN...
  6. Bilodeau P, Winistorfer S, Kearney W, Robertson A, Piper R. Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome. J Cell Biol. 2003;163:237-43 pubmed
    ..Two yeast endosomal protein complexes have been proposed as Ub-sorting "receptors," the Vps27-Hse1 complex and the ESCRT-I complex...
  7. Katzmann D, Stefan C, Babst M, Emr S. Vps27 recruits ESCRT machinery to endosomes during MVB sorting. J Cell Biol. 2003;162:413-23 pubmed
    ..Of the class E Vps proteins, both the ESCRT-I complex (composed of the class E proteins Vps23, 28, and 37) and Vps27 (mammalian hepatocyte receptor tyrosine kinase substrate, Hrs) have been shown to interact with ubiquitin, a ..
  8. Shih S, Katzmann D, Schnell J, Sutanto M, Emr S, Hicke L. Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis. Nat Cell Biol. 2002;4:389-93 pubmed
    ..UIM domains are found in a broad spectrum of proteins, consistent with the idea that mono-ubiquitin can function as a regulatory signal to control diverse biological activities. ..
  9. Ren X, Hurley J. VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo. EMBO J. 2010;29:1045-54 pubmed publisher
    VHS (Vps27, Hrs, and STAM) domains occur in ESCRT-0 subunits Hrs and STAM, GGA adapters, and other trafficking proteins. The structure of the STAM VHS domain-ubiquitin complex was solved at 2...

More Information

Publications44

  1. Bilodeau P, Urbanowski J, Winistorfer S, Piper R. The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting. Nat Cell Biol. 2002;4:534-9 pubmed
    ..These data support a model in which the Vps27p-Hse1p complex has multiple functions at the endosome, one of which is as a sorting receptor for ubiquitinated membrane proteins destined for degradation. ..
  2. Stringer D, Piper R. A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination. J Cell Biol. 2011;192:229-42 pubmed publisher
    ..Yet, proteins fused to a single Ub moiety were efficiently delivered to the MVB lumen, which strongly indicates that a single Ub is sufficient in sorting MVBs in the absence of ESCRT ubiquitination. ..
  3. Ren X, Hurley J. Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast. EMBO J. 2011;30:2130-9 pubmed publisher
    ..structure of interacting regions of the yeast ESCRT-0 and ESCRT-I complexes reveals that PSDP motifs of the Vps27 ESCRT-0 subunit bind to a novel electropositive N-terminal site on the UEV domain of the ESCRT-I subunit Vps23 ..
  4. Burd C, Emr S. Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Mol Cell. 1998;2:157-62 pubmed
    ..Our data establish a molecular link between Vps34 PI(3)K and several FYVE domain-containing proteins (Vac1p, Vps27p) required for vacuolar/lysosomal protein trafficking. ..
  5. Jensen L, Carroll M, Hall M, Harvey C, Beese S, Culotta V. Down-regulation of a manganese transporter in the face of metal toxicity. Mol Biol Cell. 2009;20:2810-9 pubmed publisher
    ..We provide evidence that manganese starvation is sensed within the lumen of the secretory pathway, whereas manganese toxicity is sensed within an extra-Golgi/cytosolic compartment of the cell. ..
  6. Rue S, Mattei S, Saksena S, Emr S. Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting. Mol Biol Cell. 2008;19:475-84 pubmed
    ..We propose a model in which the Ist1-Did2 and Vta1-Vps60 complexes independently modulate late steps in the MVB-sorting pathway. ..
  7. Tong Z, Kim M, Pandey A, Espenshade P. Identification of candidate substrates for the Golgi Tul1 E3 ligase using quantitative diGly proteomics in yeast. Mol Cell Proteomics. 2014;13:2871-82 pubmed publisher
    ..This quantitative diGly proteomics methodology will serve as a robust platform for screening for stress conditions that require Tul1 E3 ligase activity. ..
  8. Morvan J, Rinaldi B, Friant S. Pkh1/2-dependent phosphorylation of Vps27 regulates ESCRT-I recruitment to endosomes. Mol Biol Cell. 2012;23:4054-64 pubmed publisher
    ..The ESCRT-0 subunit Vps27 is a key player in this pathway since it recruits the other complexes to endosomes...
  9. Pérez Sampietro M, Herrero E. The PacC-family protein Rim101 prevents selenite toxicity in Saccharomyces cerevisiae by controlling vacuolar acidification. Fungal Genet Biol. 2014;71:76-85 pubmed publisher
    ..In addition, a parallel Rim101-independent pathway requiring the complete ESCRT machinery (including the ESCRT-0 complex) also participates in protection against selenite. ..
  10. Erpapazoglou Z, Dhaoui M, Pantazopoulou M, Giordano F, Mari M, Leon S, et al. A dual role for K63-linked ubiquitin chains in multivesicular body biogenesis and cargo sorting. Mol Biol Cell. 2012;23:2170-83 pubmed publisher
    ..A failure to generate K63Ub chains in yeast leads to an MVB ultrastructure alteration. Our work thus unravels a double function of K63Ub chains in cargo sorting and MVB biogenesis. ..
  11. Gabriely G, Kama R, Gerst J. Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast. Mol Cell Biol. 2007;27:526-40 pubmed
    ..Third, we observed physical interactions and colocalization between COPIb subunits and an MVB-associated protein, Vps27. Together, our findings suggest that certain COPI subunits could have a direct role in vacuolar protein sorting to ..
  12. Isasa M, Suñer C, Díaz M, Puig Sàrries P, Zuin A, Bichman A, et al. Cold Temperature Induces the Reprogramming of Proteolytic Pathways in Yeast. J Biol Chem. 2016;291:1664-75 pubmed publisher
    ..These unanticipated observations indicate that, during cold response, there is a proteolytic cellular reprogramming in which the proteasome acquires a role in the endocytic-vacuolar pathway. ..
  13. Eugster A, Pécheur E, Michel F, Winsor B, Letourneur F, Friant S. Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body. Mol Biol Cell. 2004;15:3031-41 pubmed
    ..Therefore, Ent3p and Ent5p are the first proteins shown to be connectors between PtdIns(3,5)P(2)- and the Vps27p-ubiquitin-driven sorting machinery at the multivesicular body. ..
  14. Swaminathan S, Amerik A, Hochstrasser M. The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeast. Mol Biol Cell. 1999;10:2583-94 pubmed
    ..We propose that Doa4 helps recycle ubiquitin from both proteasome-bound ubiquitinated intermediates and membrane proteins destined for destruction in the vacuole. ..
  15. Swanson K, Kang R, Stamenova S, Hicke L, Radhakrishnan I. Solution structure of Vps27 UIM-ubiquitin complex important for endosomal sorting and receptor downregulation. EMBO J. 2003;22:4597-606 pubmed
    ..The yeast Vps27 protein requires two UIMs for efficient interactions with ubiquitin and for sorting cargo into multivesicular ..
  16. Peters T, Miller A, Tourette C, Agren H, Hubbard A, Hughes R. Genomic Analysis of ATP Efflux in Saccharomyces cerevisiae. G3 (Bethesda). 2015;6:161-70 pubmed publisher
    ..These results will facilitate analysis of ATP efflux mechanisms in higher eukaryotes. ..
  17. Amerik A, Nowak J, Swaminathan S, Hochstrasser M. The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell. 2000;11:3365-80 pubmed
  18. Mitsui K, Koshimura Y, Yoshikawa Y, Matsushita M, Kanazawa H. The endosomal Na(+)/H(+) exchanger contributes to multivesicular body formation by regulating the recruitment of ESCRT-0 Vps27p to the endosomal membrane. J Biol Chem. 2011;286:37625-38 pubmed publisher
    ..Taken together, we propose that Nhx1p contributes to MVB formation by the recruitment of Vps27p to the endosomal membrane, possibly through Nhx1p antiporter activity. ..
  19. Dieckmann A, Babin V, Harari Y, Eils R, König R, Luke B, et al. Role of the ESCRT Complexes in Telomere Biology. MBio. 2016;7: pubmed publisher
    ..We discuss the significance of these findings and how they could be relevant to anticancer therapies. ..
  20. Mageswaran S, Johnson N, Odorizzi G, Babst M. Constitutively active ESCRT-II suppresses the MVB-sorting phenotype of ESCRT-0 and ESCRT-I mutants. Mol Biol Cell. 2015;26:554-68 pubmed publisher
    ..However, the ESCRT-III/Vps4 system alone is not sufficient for ILV formation but requires cargo sorting mediated by one of the early ESCRTs. ..
  21. McMurray M, Stefan C, Wemmer M, Odorizzi G, Emr S, Thorner J. Genetic interactions with mutations affecting septin assembly reveal ESCRT functions in budding yeast cytokinesis. Biol Chem. 2011;392:699-712 pubmed publisher
  22. Prag G, Watson H, Kim Y, Beach B, Ghirlando R, Hummer G, et al. The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting. Dev Cell. 2007;12:973-86 pubmed
    The yeast Vps27/Hse1 complex and the homologous mammalian Hrs/STAM complex deliver ubiquitinated transmembrane proteins to the ESCRT endosomal-sorting pathway...
  23. Manczyk N, Yates B, Veggiani G, Ernst A, Sicheri F, Sidhu S. Structural and functional characterization of a ubiquitin variant engineered for tight and specific binding to an alpha-helical ubiquitin interacting motif. Protein Sci. 2017;26:1060-1069 pubmed publisher
    ..Here we used phage display to generate ubiquitin variants (UbVs) targeting the N-terminal UIM of the yeast Vps27 protein. Selections yielded UbV.v27...
  24. Valdivia R, Baggott D, Chuang J, Schekman R. The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins. Dev Cell. 2002;2:283-94 pubmed
    ..Similarly, in AP-1 deficient cells, the resident TGN/early endosome syntaxin, Tlg1p, is missorted. We propose that clathrin and AP-1 act to recycle Chs3p and Tlg1p from the early endosome to the TGN. ..
  25. Kucharczyk R, Hoffman Sommer M, Piekarska I, von Mollard G, Rytka J. The Saccharomyces cerevisiae protein Ccz1p interacts with components of the endosomal fusion machinery. FEMS Yeast Res. 2009;9:565-73 pubmed publisher
    ..The genes MON1 and YPT7 also interact genetically with PEP12. These results suggest that the Ccz1p-Mon1p-Ypt7p complex is involved in fusion of transport vesicles to multiple target membranes in yeast cells. ..
  26. Arcones I, Sacristan C, Roncero C. Maintaining protein homeostasis: early and late endosomal dual recycling for the maintenance of intracellular pools of the plasma membrane protein Chs3. Mol Biol Cell. 2016;27:4021-4032 pubmed
  27. Strochlic T, Schmiedekamp B, Lee J, Katzmann D, Burd C. Opposing activities of the Snx3-retromer complex and ESCRT proteins mediate regulated cargo sorting at a common endosome. Mol Biol Cell. 2008;19:4694-706 pubmed publisher
    ..and absence of iron, Fet3-Ftr1 transits an endosomal compartment where a subunit of the MVB sorting receptor (Vps27), Snx3/Grd19, and retromer proteins colocalize...
  28. Rubio Texeira M, Van Zeebroeck G, Thevelein J. Peptides induce persistent signaling from endosomes by a nutrient transceptor. Nat Chem Biol. 2012;8:400-8 pubmed publisher
    ..Hence, this work has identified specific dipeptides that cause enhanced proton influx through the Gap1 symporter, resulting in its defective vacuolar sorting, and independently transform it into a persistently signaling transceptor. ..
  29. Wang S, Thibault G, Ng D. Routing misfolded proteins through the multivesicular body (MVB) pathway protects against proteotoxicity. J Biol Chem. 2011;286:29376-87 pubmed publisher
    ..Eliminating the MVB sorting signal diverted molecules to the vacuolar limiting membrane, resulting in the generation of toxic by-products. These data demonstrate a new role of the MVB pathway in protein quality control. ..
  30. Libuda D, Winston F. Alterations in DNA replication and histone levels promote histone gene amplification in Saccharomyces cerevisiae. Genetics. 2010;184:985-97 pubmed publisher
    ..Taken together, our results suggest that either reduced histone levels or slowed replication forks stimulate the HTA2-HTB2 amplification event, contributing to the restoration of normal chromatin structure. ..
  31. Auld K, Hitchcock A, Doherty H, Frietze S, Huang L, Silver P. The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae. Genetics. 2006;174:215-27 pubmed
    ..In combination, our characterization of GET3 genetic and biochemical interactions with NPL4, GET1, and GET2 implicates Get3 in multiple membrane-dependent pathways. ..
  32. Ha S, Bunch J, Hama H, DeWald D, Nothwehr S. A novel mechanism for localizing membrane proteins to yeast trans-Golgi network requires function of synaptojanin-like protein. Mol Biol Cell. 2001;12:3175-90 pubmed
    ..In vps27 strains defective for both anterograde and retrograde transport out of the PVC, a loss of Inp53p function markedly ..
  33. Luo W, Chang A. Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol. 1997;138:731-46 pubmed
    ..Thus, cell surface delivery of mutant Pma1 can occur as a consequence of disturbances at several different sites in the endosomal system. ..
  34. Russell M, Shideler T, Nickerson D, West M, Odorizzi G. Class E compartments form in response to ESCRT dysfunction in yeast due to hyperactivity of the Vps21 Rab GTPase. J Cell Sci. 2012;125:5208-20 pubmed publisher
    ..Indeed, genetic disruption of Rab conversion without ESCRT dysfunction autonomously drives the class E compartment morphology without blocking ILV budding. ..
  35. Bowers K, Lottridge J, Helliwell S, Goldthwaite L, Luzio J, Stevens T. Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic. 2004;5:194-210 pubmed
    ..We propose the formation of a large multimeric complex on the endosome membrane consisting of ESCRTI, ESCRTII, ESCRTIII and other associated proteins. ..