Gene Symbol: VMA16
Description: H(+)-transporting V0 sector ATPase subunit c''
Alias: PPA1, H(+)-transporting V0 sector ATPase subunit c''
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Stevens T, Forgac M. Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol. 1997;13:779-808 pubmed
    ..Recent information concerning targeting and regulation of V-ATPases has also been obtained. ..
  2. Flannery A, Graham L, Stevens T. Topological characterization of the c, c', and c" subunits of the vacuolar ATPase from the yeast Saccharomyces cerevisiae. J Biol Chem. 2004;279:39856-62 pubmed
    ..Furthermore, a chimeric fusion between Vma16p and Vma3p, Vma16-Vma3p, was found to assemble into a fully functional V-ATPase complex, further supporting the conclusion that the ..
  3. Malkus P, Graham L, Stevens T, Schekman R. Role of Vma21p in assembly and transport of the yeast vacuolar ATPase. Mol Biol Cell. 2004;15:5075-91 pubmed
  4. Finnigan G, Hanson Smith V, Stevens T, Thornton J. Evolution of increased complexity in a molecular machine. Nature. 2012;481:360-4 pubmed publisher
    ..They point to a plausible mechanism for the evolution of complexity in other multi-paralogue protein complexes. ..
  5. Tomishige N, Noda Y, Adachi H, Shimoi H, Yoda K. SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition. Yeast. 2005;22:141-55 pubmed
    ..Thus SKG1 encodes a novel protein that affects the cell wall polymer composition in the growing region of the cell. ..
  6. Whyteside G, Gibson L, Scott M, Finbow M. Assembly of the yeast vacuolar H+-ATPase and ATP hydrolysis occurs in the absence of subunit c''. FEBS Lett. 2005;579:2981-5 pubmed
    ..However, we report that subunit c'' is not essential for assembly but is for functional coupling of the enzyme. ..
  7. Hirata R, Graham L, Takatsuki A, Stevens T, Anraku Y. VMA11 and VMA16 encode second and third proteolipid subunits of the Saccharomyces cerevisiae vacuolar membrane H+-ATPase. J Biol Chem. 1997;272:4795-803 pubmed
    ..Recently, two VMA3 homologues, VMA11 and VMA16 (PPA1), have been identified in yeast, and VMA11 has been shown to be required for the V-ATPase activity...
  8. Numrich J, Péli Gulli M, Arlt H, Sardu A, Griffith J, Levine T, et al. The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis. J Cell Sci. 2015;128:2278-92 pubmed publisher
    ..Our data, thus, reveal that Ivy1 is a novel regulator of vacuole membrane homeostasis with connections to TORC1 signaling. ..
  9. Couoh Cardel S, Milgrom E, Wilkens S. Affinity Purification and Structural Features of the Yeast Vacuolar ATPase Vo Membrane Sector. J Biol Chem. 2015;290:27959-71 pubmed publisher

More Information


  1. Powell B, Graham L, Stevens T. Molecular characterization of the yeast vacuolar H+-ATPase proton pore. J Biol Chem. 2000;275:23654-60 pubmed
    ..Structural differences within the membrane-spanning domains of both V(0) and F(0) may account for the unique properties of the ATP-hydrolyzing V-ATPase compared with the ATP-generating F-type ATP synthase. ..
  2. Ryan M, Graham L, Stevens T. Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum. Mol Biol Cell. 2008;19:5131-42 pubmed publisher
    ..Our results suggest Voa1p functions with Vma21p early in V(0) assembly in the ER, but then it dissociates before exit of the V(0)-Vma21p complex from the ER for transport to the Golgi compartment. ..
  3. Diepholz M, Venzke D, Prinz S, Batisse C, Flörchinger B, Rössle M, et al. A different conformation for EGC stator subcomplex in solution and in the assembled yeast V-ATPase: possible implications for regulatory disassembly. Structure. 2008;16:1789-98 pubmed publisher
    ..However, the relative arrangement of the EG and C subunits in solution is more open than that in the holoenzyme, suggesting a conformational change of EGC during regulatory assembly and disassembly. ..
  4. Ligr M, Velten I, Frohlich E, Madeo F, Ledig M, Fröhlich K, et al. The proteasomal substrate Stm1 participates in apoptosis-like cell death in yeast. Mol Biol Cell. 2001;12:2422-32 pubmed
    ..Conversely, Stm1 accumulation induces cell death. In addition we identified five other genes whose overexpression in proteasomal mutants caused similar apoptotic phenotypes. ..