Gene Symbol: VAC8
Description: protein anchor VAC8
Alias: YEB3, protein anchor VAC8
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Fleckenstein D, Rohde M, Klionsky D, Rudiger M. Yel013p (Vac8p), an armadillo repeat protein related to plakoglobin and importin alpha is associated with the yeast vacuole membrane. J Cell Sci. 1998;111 ( Pt 20):3109-18 pubmed
    ..Our data provide evidence for the involvement of an arm-family member in vacuolar morphology and protein targeting to the vacuole. ..
  2. Wang Y, Zhao H, Harding T, Gomes de Mesquita D, Woldringh C, Klionsky D, et al. Multiple classes of yeast mutants are defective in vacuole partitioning yet target vacuole proteins correctly. Mol Biol Cell. 1996;7:1375-89 pubmed
    ..They define five complementation groups (VAC8-VAC12)...
  3. Pan X, Goldfarb D. YEB3/VAC8 encodes a myristylated armadillo protein of the Saccharomyces cerevisiae vacuolar membrane that functions in vacuole fusion and inheritance. J Cell Sci. 1998;111 ( Pt 15):2137-47 pubmed
    ..b>YEB3 encodes an uncharacterized Saccharomyces cerevisiae protein that contains eleven tandem Arm repeats...
  4. Pan X, Roberts P, Chen Y, Kvam E, Shulga N, Huang K, et al. Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p. Mol Biol Cell. 2000;11:2445-57 pubmed
    ..GFP-tagged nuclear pore complexes (NPCs) were excluded from NV junctions. In vac8-Delta cells, Nvj1p-GFP generally failed to concentrate into rafts and, instead, encircled the nucleus...
  5. Veit M, Laage R, Dietrich L, Wang L, Ungermann C. Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion. EMBO J. 2001;20:3145-55 pubmed
    ..During or after SNARE complex disassembly, palmitoylation occurs and anchors Vac8p to the vacuolar membrane. We propose that palmitoylation of Vac8p is regulated by the same machinery that controls membrane fusion. ..
  6. Wang Y, Kauffman E, Duex J, Weisman L. Fusion of docked membranes requires the armadillo repeat protein Vac8p. J Biol Chem. 2001;276:35133-40 pubmed
    ..We propose that Vac8p may bind the fusion machinery through its armadillo repeats and that palmitoylation brings this machinery to a specialized lipid domain that facilitates bilayer mixing. ..
  7. Dietrich L, Gurezka R, Veit M, Ungermann C. The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion. EMBO J. 2004;23:45-53 pubmed
    ..Sec18 is also required for palmitoylation of the fusion factor Vac8, although the acylation machinery has not been identified...
  8. Wang Y, Catlett N, Weisman L. Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. J Cell Biol. 1998;140:1063-74 pubmed
    ..This process requires actin, profilin, an unconventional myosin (Myo2p), and Vac8p. A mutant yeast strain, vac8, is defective in vacuole inheritance, specifically, in early vacuole migration...
  9. Meiringer C, Auffarth K, Hou H, Ungermann C. Depalmitoylation of Ykt6 prevents its entry into the multivesicular body pathway. Traffic. 2008;9:1510-21 pubmed publisher
    ..Thus, depalmitoylation and release of Ykt6 are needed for its recycling and to circumvent its entry into the endosomal multivesicular body pathway. ..

More Information


  1. John Peter A, Lachmann J, Rana M, Bunge M, Cabrera M, Ungermann C. The BLOC-1 complex promotes endosomal maturation by recruiting the Rab5 GTPase-activating protein Msb3. J Cell Biol. 2013;201:97-111 pubmed publisher
    ..We thus conclude that BLOC-1 controls the lifetime of active Rab5/Vps21 and thus endosomal maturation along the endocytic pathway. ..
  2. Yorimitsu T, He C, Wang K, Klionsky D. Tap42-associated protein phosphatase type 2A negatively regulates induction of autophagy. Autophagy. 2009;5:616-24 pubmed
    ..Furthermore, inactivation of PP2A stimulated autophagy and overexpression of a catalytic subunit of PP2A blocked rapamycin-induced autophagy. Our data support a model in which autophagy is negatively regulated by the Tap42-PP2A pathway. ..
  3. Peng Y, Tang F, Weisman L. Palmitoylation plays a role in targeting Vac8p to specific membrane subdomains. Traffic. 2006;7:1378-87 pubmed
    ..In addition, our studies provide evidence that palmitoylation targets Vac8p to specific membrane subdomains. ..
  4. Maeda Y, Oku M, Sakai Y. A defect of the vacuolar putative lipase Atg15 accelerates degradation of lipid droplets through lipolysis. Autophagy. 2015;11:1247-58 pubmed publisher
    ..Our data provide evidence for a novel link between autophagic flux and LD dynamics integrated with Atg15 activity. ..
  5. Umekawa M, Klionsky D. Ksp1 kinase regulates autophagy via the target of rapamycin complex 1 (TORC1) pathway. J Biol Chem. 2012;287:16300-10 pubmed publisher
    ..Our study therefore identifies Ksp1 as a new component that functions as part of the PKA and TORC1 signaling network to control the magnitude of autophagy. ..
  6. Xu H, Wickner W. Bem1p is a positive regulator of the homotypic fusion of yeast vacuoles. J Biol Chem. 2006;281:27158-66 pubmed
    ..2005) Genes Dev. 19, 2606-2618), we did not find phosphorylation of Bem1p at Ser-72 to be required for Bem1p-stimulated fusion. Taken together, Bem1p is a positive regulator of lipid mixing during vacuole hemifusion and fusion. ..
  7. Tang F, Peng Y, Nau J, Kauffman E, Weisman L. Vac8p, an armadillo repeat protein, coordinates vacuole inheritance with multiple vacuolar processes. Traffic. 2006;7:1368-77 pubmed
    ..These findings suggest that a major role of Vac8p is to spatially separate multiple functions thereby enabling vacuole inheritance to occur concurrently with other vacuolar processes. ..
  8. Cheong H, Yorimitsu T, Reggiori F, Legakis J, Wang C, Klionsky D. Atg17 regulates the magnitude of the autophagic response. Mol Biol Cell. 2005;16:3438-53 pubmed
    ..As a result, it is defective in peroxisome degradation and is partially defective for autophagy. Atg17 interacts with both Atg1 and Atg13, via two coiled-coil domains, and these interactions facilitate its inclusion in the Atg1 complex. ..
  9. Dietrich L, Ungermann C. On the mechanism of protein palmitoylation. EMBO Rep. 2004;5:1053-7 pubmed
    ..Recently, we discovered that Ykt6 mediates the amino-terminal acylation of the fusion protein Vac8. Even though these three proteins differ in sequence, topology, size and substrate specificity, they might function ..
  10. Lopreiato R, Facchin S, Sartori G, Arrigoni G, Casonato S, Ruzzene M, et al. Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. Biochem J. 2004;377:395-405 pubmed
    ..The functional significance of the interaction between Bud32 and the putative protease Ykr038/Kae1 is supported by its evolutionary conservation. ..
  11. Scott S, Nice D, Nau J, Weisman L, Kamada Y, Keizer Gunnink I, et al. Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J Biol Chem. 2000;275:25840-9 pubmed
    ..The Vac8 and Apg13 proteins are required for the import of aminopeptidase I (API) through the Cvt pathway...
  12. Subramanian K, Dietrich L, Hou H, LaGrassa T, Meiringer C, Ungermann C. Palmitoylation determines the function of Vac8 at the yeast vacuole. J Cell Sci. 2006;119:2477-85 pubmed
    ..The yeast protein Vac8 is required for efficient vacuole fusion, inheritance and cytosol-to-vacuole trafficking...
  13. Tang F, Kauffman E, Novak J, Nau J, Catlett N, Weisman L. Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature. 2003;422:87-92 pubmed
    ..Thus the regulated disruption of this transport complex places the vacuole in its proper location. This may be a general mechanism whereby organelles are deposited at their terminal destination. ..
  14. Kraft C, Kijanska M, Kalie E, Siergiejuk E, Lee S, Semplicio G, et al. Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy. EMBO J. 2012;31:3691-703 pubmed publisher
    ..Together, these findings define a conserved step in autophagy regulation in yeast and mammals and expand the known functions of LIR-dependent Atg8 targets to include spatial regulation of the Atg1/ULK1 kinase. ..
  15. Zurita Martinez S, Puria R, Pan X, Boeke J, Cardenas M. Efficient Tor signaling requires a functional class C Vps protein complex in Saccharomyces cerevisiae. Genetics. 2007;176:2139-50 pubmed
  16. Tang F, Watkins J, Bermudez M, Gray R, Gaban A, Portie K, et al. A life-span extending form of autophagy employs the vacuole-vacuole fusion machinery. Autophagy. 2008;4:874-86 pubmed
    ..Pending future biochemical studies, the concept of secretophagy may provide a mechanism for autophagy in life-span extension. ..
  17. Nadolski M, Linder M. Molecular recognition of the palmitoylation substrate Vac8 by its palmitoyltransferase Pfa3. J Biol Chem. 2009;284:17720-30 pubmed publisher
    Palmitoylation of the yeast vacuolar protein Vac8 is important for its role in membrane-mediated events such as vacuole fusion...
  18. Hou H, John Peter A, Meiringer C, Subramanian K, Ungermann C. Analysis of DHHC acyltransferases implies overlapping substrate specificity and a two-step reaction mechanism. Traffic. 2009;10:1061-73 pubmed publisher
    ..For Pfa3 and its substrate Vac8, we can distinguish two consecutive steps in the acylation reaction: an initial binding that occurs independently ..
  19. Roberts P, Moshitch Moshkovitz S, Kvam E, O TOOLE E, Winey M, Goldfarb D. Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:129-41 pubmed
    ..These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole. ..
  20. Hou H, Subramanian K, LaGrassa T, Markgraf D, Dietrich L, Urban J, et al. The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8. Proc Natl Acad Sci U S A. 2005;102:17366-71 pubmed
    ..At least three palmitoylated proteins are found exclusively on yeast vacuoles: the fusion factor Vac8, the kinase Yck3, and a novel adaptor protein implicated in microautophagy, Meh1...
  21. Smotrys J, Schoenfish M, Stutz M, Linder M. The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p. J Cell Biol. 2005;170:1091-9 pubmed
    ..Vac8p is the first N-myristoylated, palmitoylated protein identified as a substrate for a DHHC-CRD protein. ..
  22. Dietrich L, LaGrassa T, Rohde J, Cristodero M, Meiringer C, Ungermann C. ATP-independent control of Vac8 palmitoylation by a SNARE subcomplex on yeast vacuoles. J Biol Chem. 2005;280:15348-55 pubmed
    Yeast vacuole fusion requires palmitoylated Vac8. We previously showed that Vac8 acylation occurs early in the fusion reaction, is blocked by antibodies against Sec18 (yeast N-ethylmaleimide-sensitive fusion protein (NSF)), and is ..