USO1

Summary

Gene Symbol: USO1
Description: Uso1p
Alias: INT1, Uso1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Yamakawa H, Seog D, Yoda K, Yamasaki M, Wakabayashi T. Uso1 protein is a dimer with two globular heads and a long coiled-coil tail. J Struct Biol. 1996;116:356-65 pubmed
    b>USO1 is one of the essential genes in Saccharomyces cerevisiae whose gene products participate in protein transport from the endoplasmic reticulum to the Golgi apparatus. This product was purified to homogeneity...
  2. Cao X, Ballew N, Barlowe C. Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J. 1998;17:2156-65 pubmed
    ER-to-Golgi transport in yeast may be reproduced in vitro with washed membranes, purified proteins (COPII, Uso1p and LMA1) and energy...
  3. VanRheenen S, Cao X, Lupashin V, Barlowe C, Waters M. Sec35p, a novel peripheral membrane protein, is required for ER to Golgi vesicle docking. J Cell Biol. 1998;141:1107-19 pubmed
    ..suggest that Sec35p acts upstream of, or in conjunction with, Ypt1p and Sly1p as was previously found for Uso1p. Using a cell-free assay that measures distinct steps in vesicle transport from the ER to the Golgi, we find ..
  4. Jiang Y, Scarpa A, Zhang L, Stone S, Feliciano E, Ferro Novick S. A high copy suppressor screen reveals genetic interactions between BET3 and a new gene. Evidence for a novel complex in ER-to-Golgi transport. Genetics. 1998;149:833-41 pubmed
    ..of the bet5-1 mutant include several genes whose products are required for ER-to-Golgi transport (BET1, SEC22, USO1 and DSS4) and the maintenance of the Golgi (ANP1)...
  5. Ballew N, Liu Y, Barlowe C. A Rab requirement is not bypassed in SLY1-20 suppression. Mol Biol Cell. 2005;16:1839-49 pubmed
    The Rab GTPase Ypt1p and the large homodimer Uso1p are both required for tethering endoplasmic reticulum-derived vesicles to early Golgi compartments in yeast...
  6. Sapperstein S, Lupashin V, Schmitt H, Waters M. Assembly of the ER to Golgi SNARE complex requires Uso1p. J Cell Biol. 1996;132:755-67 pubmed
    b>Uso1p, a Saccharomyces cerevisiae protein required for ER to Golgi transport, is homologous to the mammalian intra-Golgi transport factor p115. We have used genetic and biochemical approaches to examine the function of Uso1p...
  7. Kim D, Sacher M, Scarpa A, Quinn A, Ferro Novick S. High-copy suppressor analysis reveals a physical interaction between Sec34p and Sec35p, a protein implicated in vesicle docking. Mol Biol Cell. 1999;10:3317-29 pubmed
    ..Sec34p and Sec35p stably associate with each other to form a multiprotein complex of approximately 480 kDa. These data indicate that Sec34p acts in conjunction with Sec35p to mediate a common step in vesicular traffic. ..
  8. VanRheenen S, Reilly B, Chamberlain S, Waters M. Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast. Traffic. 2001;2:212-31 pubmed
  9. Kosodo Y, Imai K, Hirata A, Noda Y, Takatsuki A, Adachi H, et al. Multicopy suppressors of the sly1 temperature-sensitive mutation in the ER-Golgi vesicular transport in Saccharomyces cerevisiae. Yeast. 2001;18:1003-14 pubmed
    ..shock proteins, SSB1 and SSB2; (3) cell surface proteins, WSC1, WSC2 and MID2; (4) ER-Golgi transport proteins, USO1 and BET1; and (5) an as-yet-uncharacterized protein, HSD1 (high-copy suppressor of SLY1 defect 1)...

More Information

Publications18

  1. Mukherjee I, Barlowe C. Overexpression of Sly41 suppresses COPII vesicle-tethering deficiencies by elevating intracellular calcium levels. Mol Biol Cell. 2016;27:1635-49 pubmed publisher
    ..These results show that calcium positively regulates the SNARE-dependent fusion stage of ER-Golgi transport. ..
  2. Behnia R, Barr F, Flanagan J, Barlowe C, Munro S. The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic. J Cell Biol. 2007;176:255-61 pubmed
    ..essential for growth, but biochemical assays and genetic interactions with known mediators of vesicle tethering (Uso1 and Ypt1) suggest that the Grh1-Bug1 complex contributes to a redundant network of interactions that mediates ..
  3. Heidtman M, Chen C, Collins R, Barlowe C. A role for Yip1p in COPII vesicle biogenesis. J Cell Biol. 2003;163:57-69 pubmed
    ..We propose that Yip1p has a previously unappreciated role in COPII vesicle biogenesis. ..
  4. Kim D. Characterization of Grp1p, a novel cis-Golgi matrix protein. Biochem Biophys Res Commun. 2003;303:370-8 pubmed
    ..5h incubation at 38.5 degrees C. Together, these data suggest that Grp1p is a novel matrix protein that is involved in the structural organization of the cis-Golgi. ..
  5. Luo Z, Gallwitz D. Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments. J Biol Chem. 2003;278:791-9 pubmed
    ..The results obtained indicate that Ypt6p acts in endosome-to-Golgi, in intra-Golgi retrograde transport, and possibly also in Golgi-to-ER trafficking. ..
  6. VanRheenen S, Cao X, Sapperstein S, Chiang E, Lupashin V, Barlowe C, et al. Sec34p, a protein required for vesicle tethering to the yeast Golgi apparatus, is in a complex with Sec35p. J Cell Biol. 1999;147:729-42 pubmed
    ..Weaker suppression is evident upon overexpression of genes encoding the vesicle tethering factor Uso1p or the vesicle-SNAREs Sec22p, Bet1p, or Ykt6p...
  7. Kito M, Seog D, Igarashi K, Kambe Honjo H, Yoda K, Yamasaki M. Calcium and SLY genes suppress the temperature-sensitive secretion defect of Saccharomyces cerevisiae uso1 mutant. Biochem Biophys Res Commun. 1996;220:653-7 pubmed
    Saccharomyces cerevisiae uso1-1 mutant stops the transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus at 37 degrees C...
  8. Dechamps C, Portetelle D, Vandenbol M. Multicopy suppression screen in the msb3 msb4 Saccharomyces cerevisiae double mutant, affected in Ypt/RabGAP activity. Biotechnol Lett. 2005;27:1439-49 pubmed
    ..classified on the basis of functional features into four groups: vesicular transport proteins (Sec7p, Vps35p, and Uso1p), a protein involved in cell division (Sap155p), a molecular chaperon (Ssz1p), and a protein associated with the ..
  9. Noda Y, Yamagishi T, Yoda K. Specific membrane recruitment of Uso1 protein, the essential endoplasmic reticulum-to-Golgi tethering factor in yeast vesicular transport. J Cell Biochem. 2007;101:686-94 pubmed
    b>Uso1 is a yeast essential protein that functions to tether vesicles in the ER-to-Golgi transport. Its recruitment to the ER-derived vesicles has been demonstrated in in vitro membrane transport systems using semi-intact cells...