URM1

Summary

Gene Symbol: URM1
Description: ubiquitin-related modifier URM1
Alias: ubiquitin-related modifier URM1
Species: Saccharomyces cerevisiae S288c
Products:     URM1

Top Publications

  1. Goehring A, Rivers D, Sprague G. Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell. 2003;14:4329-41 pubmed
    ..Moreover, these five genes have a role in invasive growth and display genetic interactions with the TOR pathway. In summary, our results suggest the urmylation pathway is involved in nutrient sensing and budding. ..
  2. Goehring A, Rivers D, Sprague G. Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p. Eukaryot Cell. 2003;2:930-6 pubmed
    ..These results suggest that the conjugation of Urm1p to Ahp1p could regulate the function of Ahp1p in antioxidant stress response in Saccharomyces cerevisiae. ..
  3. Furukawa K, Mizushima N, Noda T, Ohsumi Y. A protein conjugation system in yeast with homology to biosynthetic enzyme reaction of prokaryotes. J Biol Chem. 2000;275:7462-5 pubmed
    ..Here we report on a novel protein conjugation system in yeast. A newly identified ubiquitin related modifier, Urm1 is a 99-amino acid protein terminated with glycine-glycine...
  4. Rubio Texeira M. Urmylation controls Nil1p and Gln3p-dependent expression of nitrogen-catabolite repressed genes in Saccharomyces cerevisiae. FEBS Lett. 2007;581:541-50 pubmed
    b>Urm1 is a modifier protein that is conjugated to substrate proteins through thioester formation with the E1-like enzyme, Uba4...
  5. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..In addition to earlier described mutants, formation of the s(2) group was also abolished in urm1, uba4, and ncs2 mutants and decreased in the yor251c mutant...
  6. Xu J, Zhang J, Wang L, Zhou J, Huang H, Wu J, et al. Solution structure of Urm1 and its implications for the origin of protein modifiers. Proc Natl Acad Sci U S A. 2006;103:11625-30 pubmed
    ..between the protein modifiers and sulfur carrier proteins, we solved the solution NMR structure of the Urm1 (ubiquitin-related modifier-1) protein from Saccharomyces cerevisiae...
  7. van der Veen A, Schorpp K, Schlieker C, Buti L, Damon J, Spooner E, et al. Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier. Proc Natl Acad Sci U S A. 2011;108:1763-70 pubmed publisher
    The ubiquitin (Ub)-related modifier Urm1 functions as a sulfur carrier in tRNA thiolation by means of a mechanism that requires the formation of a thiocarboxylate at the C-terminal glycine residue of Urm1...
  8. Nakai Y, Nakai M, Hayashi H. Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems. J Biol Chem. 2008;283:27469-76 pubmed publisher
    ..We found that ubiquitin-like protein Urm1 and ubiquitin-activating enzyme-like protein Uba4, as well as Tuc1 and Tuc2, were strictly required for the s(2) ..
  9. Jüdes A, Ebert F, Bär C, Thüring K, Harrer A, Klassen R, et al. Urmylation and tRNA thiolation functions of ubiquitin-like Uba4·Urm1 systems are conserved from yeast to man. FEBS Lett. 2015;589:904-9 pubmed publisher
    The ubiquitin-like protein Urm1 from budding yeast and its E1-like activator Uba4 have dual roles in protein urmylation and tRNA thiolation pathways...

More Information

Publications15

  1. Petroski M, Salvesen G, Wolf D. Urm1 couples sulfur transfer to ubiquitin-like protein function in oxidative stress. Proc Natl Acad Sci U S A. 2011;108:1749-50 pubmed publisher
  2. Damon J, Pincus D, Ploegh H. tRNA thiolation links translation to stress responses in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:270-82 pubmed publisher
    ..The URM1 pathway is required for thiolation of the cytoplasmic tRNAs tGlu(UUC), tGln(UUG), and tLys(UUU) in Saccharomyces ..
  3. Klassen R, Grunewald P, Thüring K, Eichler C, Helm M, Schaffrath R. Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae. PLoS ONE. 2015;10:e0119261 pubmed publisher
    ..which disable mcm5 side chain formation, or removal of components of the thiolation pathway (Ncs2/Ncs6, Urm1, Uba4) are individually tolerated, the combination of both modification defects has been reported to have lethal ..
  4. Noma A, Sakaguchi Y, Suzuki T. Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res. 2009;37:1335-52 pubmed publisher
    ..We have identified five genes in Saccharomyces cerevisiae, YIL008w (URM1), YHR111w (UBA4), YOR251c (TUM1), YNL119w (NCS2) and YGL211w (NCS6), that are required for 2-thiolation of mcm(5)s(..
  5. Scheidt V, Jüdes A, Bär C, Klassen R, Schaffrath R. Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling. Microb Cell. 2014;1:416-424 pubmed publisher
    ..i>sap190?, ppm1?, rrd1?) and U34 modification defects (elp3?, kti12?, urm1?, ncs2?) and found the rapamycin hypersensitivity in the latter is epistatic to drug resistance of the ..
  6. Yu J, Zhou C. Crystal structure of the dimeric Urm1 from the yeast Saccharomyces cerevisiae. Proteins. 2008;71:1050-5 pubmed publisher