URE2

Summary

Gene Symbol: URE2
Description: Ure2p
Alias: Ure2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cunningham T, Andhare R, Cooper T. Nitrogen catabolite repression of DAL80 expression depends on the relative levels of Gat1p and Ure2p production in Saccharomyces cerevisiae. J Biol Chem. 2000;275:14408-14 pubmed
    ..b>Ure2p, which is not a GATA family member, inhibits Gln3p/Gat1p from functioning in the presence of good nitrogen sources...
  2. Basu U, Southron J, Stephens J, Taylor G. Reverse genetic analysis of the glutathione metabolic pathway suggests a novel role of PHGPX and URE2 genes in aluminum resistance in Saccharomyces cerevisiae. Mol Genet Genomics. 2004;271:627-37 pubmed
    ..Interestingly, a strain deleted for URE2, a gene which encodes a prion precursor with homology to GSTs, also showed hypersensitivity to Al...
  3. Lian H, Jiang Y, Zhang H, Jones G, Perrett S. The yeast prion protein Ure2: structure, function and folding. Biochim Biophys Acta. 2006;1764:535-45 pubmed
    The Saccharomyces cerevisiae protein Ure2 functions as a regulator of nitrogen metabolism and as a glutathione-dependent peroxidase...
  4. Georis I, Tate J, Cooper T, Dubois E. Tor pathway control of the nitrogen-responsive DAL5 gene bifurcates at the level of Gln3 and Gat1 regulation in Saccharomyces cerevisiae. J Biol Chem. 2008;283:8919-29 pubmed publisher
    ..Gln3-Myc(13) localization is highly dependent upon Ure2 and type 2A-related phosphatase, Sit4...
  5. Todorova T, Kujumdzieva A, Vuilleumier S. Non-enzymatic roles for the URE2 glutathione S-transferase in the response of Saccharomyces cerevisiae to arsenic. Arch Microbiol. 2010;192:909-18 pubmed publisher
    ..Glutathione peroxidase activity, previously reported for the Ure2p protein, was unaffected in cell-free extracts of an ure2? mutant of S. cerevisiae...
  6. Zhang H, Loovers H, Xu L, Wang M, Rowling P, Itzhaki L, et al. Alcohol oxidase (AOX1) from Pichia pastoris is a novel inhibitor of prion propagation and a potential ATPase. Mol Microbiol. 2009;71:702-16 pubmed publisher
    ..The S. cerevisiae prion states [PSI(+)] and [URE3] arise from aggregation of the proteins Sup35p and Ure2p respectively, and correlate with the ability of Sup35p and Ure2p to form amyloid-like fibrils in vitro...
  7. Ngo S, Gu L, Guo Z. Hierarchical organization in the amyloid core of yeast prion protein Ure2. J Biol Chem. 2011;286:29691-9 pubmed publisher
    ..labeling and electron paramagnetic resonance (EPR) spectroscopy to investigate the structures of amyloid fibrils of Ure2 prion domain...
  8. Brachmann A, Baxa U, Wickner R. Prion generation in vitro: amyloid of Ure2p is infectious. EMBO J. 2005;24:3082-92 pubmed
    URE3] is a prion (infectious protein) of the Ure2 protein of yeast. In vitro, Ure2p can form amyloid filaments, but direct evidence that these filaments constitute the infectious form is still missing...
  9. Ross E, Edskes H, Terry M, Wickner R. Primary sequence independence for prion formation. Proc Natl Acad Sci U S A. 2005;102:12825-30 pubmed
    ..The [URE3] and [PSI+] prions of Saccharomyces cerevisiae are infectious amyloid forms of the proteins Ure2p and Sup35p, respectively...

More Information

Publications74

  1. Pieri L, Bucciantini M, Guasti P, Savistchenko J, Melki R, Stefani M. Synthetic lipid vesicles recruit native-like aggregates and affect the aggregation process of the prion Ure2p: insights on vesicle permeabilization and charge selectivity. Biophys J. 2009;96:3319-30 pubmed publisher
    The yeast prion Ure2p polymerizes into native-like fibrils, retaining the overall structure and binding properties of the soluble protein...
  2. Cox K, Rai R, Distler M, Daugherty J, Coffman J, Cooper T. Saccharomyces cerevisiae GATA sequences function as TATA elements during nitrogen catabolite repression and when Gln3p is excluded from the nucleus by overproduction of Ure2p. J Biol Chem. 2000;275:17611-8 pubmed
    ..fluorescent protein (EGFP)-Gln3p in the cytoplasm in a way that is indistinguishable from that seen with EGFP-Ure2p. However, when the shorter, NCR-sensitive DAL5 transcript predominates, EGFP-Gln3p is nuclear...
  3. Feller A, Georis I, Tate J, Cooper T, Dubois E. Alterations in the Ure2 ?Cap domain elicit different GATA factor responses to rapamycin treatment and nitrogen limitation. J Biol Chem. 2013;288:1841-55 pubmed publisher
    b>Ure2 is a phosphoprotein and central negative regulator of nitrogen-responsive Gln3/Gat1 localization and their ability to activate transcription...
  4. Sharma D, Stanley R, Masison D. Curing of yeast [URE3] prion by the Hsp40 cochaperone Ydj1p is mediated by Hsp70. Genetics. 2009;181:129-37 pubmed publisher
    ..On the basis of biochemical data Ydj1p has been proposed to cure [URE3] by binding soluble Ure2p and preventing it from joining prion aggregates...
  5. Zhang Z, Perrett S. Novel glutaredoxin activity of the yeast prion protein Ure2 reveals a native-like dimer within fibrils. J Biol Chem. 2009;284:14058-67 pubmed publisher
    b>Ure2 is the protein determinant of the Saccharomyces cerevisiae prion [URE3]...
  6. Redeker V, Halgand F, Le Caer J, Bousset L, Laprevote O, Melki R. Hydrogen/deuterium exchange mass spectrometric analysis of conformational changes accompanying the assembly of the yeast prion Ure2p into protein fibrils. J Mol Biol. 2007;369:1113-25 pubmed
    ..In vitro, at neutral pH, soluble Ure2p forms long, twisted fibrils. Two models have been proposed to account for Ure2p polymerization...
  7. Redeker V, Bonnefoy J, Le Caer J, Pemberton S, Laprevote O, Melki R. A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry. FEBS J. 2010;277:5112-23 pubmed publisher
    ..We previously demonstrated that the molecular chaperone Ssa1p sequesters Ure2p in high molecular weight, assembly incompetent oligomeric species...
  8. Speare J, Offerdahl D, Hasenkrug A, Carmody A, Baron G. GPI anchoring facilitates propagation and spread of misfolded Sup35 aggregates in mammalian cells. EMBO J. 2010;29:782-94 pubmed publisher
    ..These data demonstrate GPI anchoring facilitates the propagation and spread of protein aggregation and thus may enhance the transmissibility and pathogenesis of prion diseases relative to other protein misfolding diseases. ..
  9. Pierce M, Baxa U, Steven A, Bax A, Wickner R. Is the prion domain of soluble Ure2p unstructured?. Biochemistry. 2005;44:321-8 pubmed
    ..Deletions in the C-terminal nitrogen regulation domain of Ure2p increase the frequency with which the N-terminal prion domain polymerizes into the prion form, suggesting that the ..
  10. Thual C, Komar A, Bousset L, Fernandez Bellot E, Cullin C, Melki R. Structural characterization of Saccharomyces cerevisiae prion-like protein Ure2. J Biol Chem. 1999;274:13666-74 pubmed
    ..We show here that Ure2p is a soluble protein that can assemble into fibers that are similar to the fibers observed in the case of PrP in ..
  11. Ranson N, Stromer T, Bousset L, Melki R, Serpell L. Insights into the architecture of the Ure2p yeast protein assemblies from helical twisted fibrils. Protein Sci. 2006;15:2481-7 pubmed
    ..Such prion properties are thought to arise from the fact that Ure2p is able to self-assemble into insoluble fibrils...
  12. Jiang Y, Li H, Zhu L, Zhou J, Perrett S. Amyloid nucleation and hierarchical assembly of Ure2p fibrils. Role of asparagine/glutamine repeat and nonrepeat regions of the prion domains. J Biol Chem. 2004;279:3361-9 pubmed
    ..The Asn/Gln region of the Ure2p prion domain extends to residue 89, but residues 15-42 represent an island of "normal" random sequence, ..
  13. Fei L, Perrett S. Disulfide bond formation significantly accelerates the assembly of Ure2p fibrils because of the proximity of a potential amyloid stretch. J Biol Chem. 2009;284:11134-41 pubmed publisher
    ..The N-terminal region of Ure2p is necessary and sufficient to induce the [URE3] phenotype in vivo and to polymerize into amyloid-like fibrils in ..
  14. Baxa U, Wickner R, Steven A, Anderson D, Marekov L, Yau W, et al. Characterization of beta-sheet structure in Ure2p1-89 yeast prion fibrils by solid-state nuclear magnetic resonance. Biochemistry. 2007;46:13149-62 pubmed
    Residues 1-89 constitute the Asn- and Gln-rich segment of the Ure2p protein and produce the [URE3] prion of Saccharomyces cerevisiae by forming the core of intracellular Ure2p amyloid...
  15. Savistchenko J, Krzewska J, Fay N, Melki R. Molecular chaperones and the assembly of the prion Ure2p in vitro. J Biol Chem. 2008;283:15732-9 pubmed publisher
    ..In vivo, a high molecular weight form of inactive Ure2p is associated to [URE3]...
  16. Bai M, Zhou J, Perrett S. The yeast prion protein Ure2 shows glutathione peroxidase activity in both native and fibrillar forms. J Biol Chem. 2004;279:50025-30 pubmed
    b>Ure2p is the precursor protein of the Saccharomyces cerevisiae prion [URE3]. Ure2p shows homology to glutathione transferases but lacks typical glutathione transferase activity...
  17. Bertram P, Choi J, Carvalho J, Ai W, Zeng C, Chan T, et al. Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases. J Biol Chem. 2000;275:35727-33 pubmed
    ..The yeast pro-prion protein Ure2p binds to both hyper- and hypo-phosphorylated Gln3p...
  18. Beck T, Hall M. The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors. Nature. 1999;402:689-92 pubmed
    ..nitrogen limitation by promoting the association of the GATA transcription factor GLN3 with the cytoplasmic protein URE2. The binding of GLN3 to URE2 requires TOR-dependent phosphorylation of GLN3...
  19. Perrett S, Freeman S, Butler P, Fersht A. Equilibrium folding properties of the yeast prion protein determinant Ure2. J Mol Biol. 1999;290:331-45 pubmed
    ..factor [URE3] propagates by a prion-like mechanism, involving aggregation of the chromosomally encoded protein Ure2. The [URE3] phenotype is equivalent to loss of function of Ure2, a protein involved in regulation of nitrogen ..
  20. Chen L, Chen L, Wang H, Wang Y, Perrett S. Deletion of a Ure2 C-terminal prion-inhibiting region promotes the rate of fibril seed formation and alters interaction with Hsp40. Protein Eng Des Sel. 2011;24:69-78 pubmed publisher
    ..b>Ure2, the nitrogen metabolism regulation factor of Saccharomyces cerevisiae, shows prion properties in vivo and forms ..
  21. Kryndushkin D, Wickner R, Tycko R. The core of Ure2p prion fibrils is formed by the N-terminal segment in a parallel cross-β structure: evidence from solid-state NMR. J Mol Biol. 2011;409:263-77 pubmed publisher
    Intracellular fibril formation by Ure2p produces the non-Mendelian genetic element [URE3] in Saccharomyces cerevisiae, making Ure2p a prion protein...
  22. Bousset L, Belrhali H, Janin J, Melki R, Morera S. Structure of the globular region of the prion protein Ure2 from the yeast Saccharomyces cerevisiae. Structure. 2001;9:39-46 pubmed
    ..cerevisiae is due to the propagation of a transmissible form of the protein Ure2. The infectivity of Ure2p is thought to originate from a conformational change of the normal form of the prion protein...
  23. Kryndushkin D, Wickner R. Nucleotide exchange factors for Hsp70s are required for [URE3] prion propagation in Saccharomyces cerevisiae. Mol Biol Cell. 2007;18:2149-54 pubmed
    The [URE3] and [PSI(+)] prions are infectious amyloid forms of Ure2p and Sup35p. Several chaperones influence prion propagation: Hsp104p overproduction destabilizes [PSI(+)], whereas [URE3] is sensitive to excess of Ssa1p or Ydj1p...
  24. Loquet A, Bousset L, Gardiennet C, Sourigues Y, Wasmer C, Habenstein B, et al. Prion fibrils of Ure2p assembled under physiological conditions contain highly ordered, natively folded modules. J Mol Biol. 2009;394:108-18 pubmed publisher
    ..NMR can unravel the atomic-resolution three-dimensional structure of prion fragments but, in the case of Ure2p, no highly resolved spectra are obtained from the isolated prion domain...
  25. Georis I, Tate J, Feller A, Cooper T, Dubois E. Intranuclear function for protein phosphatase 2A: Pph21 and Pph22 are required for rapamycin-induced GATA factor binding to the DAL5 promoter in yeast. Mol Cell Biol. 2011;31:92-104 pubmed publisher
    ..Finally, we demonstrate that a pph21? pph22? double mutant is epistatic to ure2? for nuclear Gat1 localization in untreated glutamine-grown cells, whereas for Gln3, just the opposite occurs: i.e...
  26. Zhang Z, Bai M, Wang X, Zhou J, Perrett S. "Restoration" of glutathione transferase activity by single-site mutation of the yeast prion protein Ure2. J Mol Biol. 2008;384:641-51 pubmed publisher
    The yeast prion Ure2p is composed of an N-terminal prion domain, and a C-terminal globular domain, which shows similarity to glutathione transferases (GSTs) in both sequence and structure...
  27. Blinder D, Coschigano P, Magasanik B. Interaction of the GATA factor Gln3p with the nitrogen regulator Ure2p in Saccharomyces cerevisiae. J Bacteriol. 1996;178:4734-6 pubmed
    We used cells carrying plasmids causing the overproduction of Gln3p, Ure2p, or both of these proteins to elucidate the ability of Ure2p to prevent the activation of gene expression by Gln3p in cells growing in a glutamine-containing ..
  28. Shewmaker F, Wickner R. Ageing in yeast does not enhance prion generation. Yeast. 2006;23:1123-8 pubmed
    The yeast prions [URE3] and [PSI(+)] are self-propagating amyloids of Ure2p and Sup35p, respectively. The analogous transmissible spongiform encephalopathies of mammals and other amyloidoses are largely diseases of later life...
  29. Troisi E, Rockman M, Nguyen P, Oliver E, Hines J. Swa2, the yeast homolog of mammalian auxilin, is specifically required for the propagation of the prion variant [URE3-1]. Mol Microbiol. 2015;97:926-41 pubmed publisher
  30. Higurashi T, Hines J, Sahi C, Aron R, Craig E. Specificity of the J-protein Sis1 in the propagation of 3 yeast prions. Proc Natl Acad Sci U S A. 2008;105:16596-601 pubmed publisher
  31. Kryndushkin D, Engel A, Edskes H, Wickner R. Molecular chaperone Hsp104 can promote yeast prion generation. Genetics. 2011;188:339-48 pubmed publisher
    URE3] is an amyloid-based prion of Ure2p, a regulator of nitrogen catabolism in Saccharomyces cerevisiae. The Ure2p of the human pathogen Candida albicans can also be a prion in S. cerevisiae...
  32. Georis I, Feller A, Tate J, Cooper T, Dubois E. Nitrogen catabolite repression-sensitive transcription as a readout of Tor pathway regulation: the genetic background, reporter gene and GATA factor assayed determine the outcomes. Genetics. 2009;181:861-74 pubmed publisher
    ..Together, the data indicate that in the absence of these three pieces of information, overall NCR-sensitive gene transcription data are unreliable as Tor pathway readouts. ..
  33. Fayard B, Fay N, David G, Doucet J, Melki R. Packing of the prion Ure2p in protein fibrils probed by fluorescence X-ray near-edge structure spectroscopy at sulfur K-edge. J Mol Biol. 2006;356:843-9 pubmed
    The soluble protein Ure2p from the yeast Saccharomyces cerevisiae assembles in vitro into straight and insoluble protein fibrils, through subtle changes of conformation...
  34. Zhang C, Jackson A, Zhang Z, Han Y, Yu S, He R, et al. Amyloid-like aggregates of the yeast prion protein ure2 enter vertebrate cells by specific endocytotic pathways and induce apoptosis. PLoS ONE. 2010;5: pubmed publisher
    ..However, the mechanisms involved remain poorly understood. Here we use the yeast prion protein Ure2 as a generic model to investigate how amyloid-like protein aggregates can enter mammalian cells and convey ..
  35. Oishi K, Kurahashi H, Pack C, Sako Y, Nakamura Y. A bipolar functionality of Q/N-rich proteins: Lsm4 amyloid causes clearance of yeast prions. Microbiologyopen. 2013;2:415-30 pubmed publisher
    ..We also found that the antiprion activity is a general property of [PSI(+) ]-inducible factors. These data provoked a novel "unified" model that explains both prion induction and elimination by a single scheme. ..
  36. Coffman J, el Berry H, Cooper T. The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae. J Bacteriol. 1994;176:7476-83 pubmed
    ..Here, we extend the analysis to include the response of their expression to deletion of the URE2 locus...
  37. Shewmaker F, Ross E, Tycko R, Wickner R. Amyloids of shuffled prion domains that form prions have a parallel in-register beta-sheet structure. Biochemistry. 2008;47:4000-7 pubmed publisher
    The [URE3] and [PSI (+)] prions of Saccharomyces cerevisiae are self-propagating amyloid forms of Ure2p and Sup35p, respectively...
  38. Shewmaker F, Mull L, Nakayashiki T, Masison D, Wickner R. Ure2p function is enhanced by its prion domain in Saccharomyces cerevisiae. Genetics. 2007;176:1557-65 pubmed
    The Ure2 protein of Saccharomyces cerevisiae can become a prion (infectious protein). At very low frequencies Ure2p forms an insoluble, infectious amyloid known as [URE3], which is efficiently transmitted to progeny cells or mating ..
  39. Maloney B, Ge Y, Greig N, Lahiri D. Presence of a "CAGA box" in the APP gene unique to amyloid plaque-forming species and absent in all APLP-1/2 genes: implications in Alzheimer's disease. FASEB J. 2004;18:1288-90 pubmed publisher
  40. Edskes H, Hanover J, Wickner R. Mks1p is a regulator of nitrogen catabolism upstream of Ure2p in Saccharomyces cerevisiae. Genetics. 1999;153:585-94 pubmed
    ..b>Ure2p of Saccharomyces cerevisiae is a negative regulator of nitrogen catabolism that inhibits Gln3p, a positive ..
  41. Rai R, Tate J, Shanmuganatham K, Howe M, Nelson D, Cooper T. Nuclear Gln3 Import Is Regulated by Nitrogen Catabolite Repression Whereas Export Is Specifically Regulated by Glutamine. Genetics. 2015;201:989-1016 pubmed publisher
    ..We also demonstrate that Gln3 residues 64-73 are required for nuclear Gln3 export. ..
  42. Wang Y, Bongiovanni M, Gras S, Perrett S. The fibrils of Ure2p homologs from Saccharomyces cerevisiae and Saccharoymyces paradoxus have similar cross-? structure in both dried and hydrated forms. J Struct Biol. 2011;174:505-11 pubmed publisher
    ..Given the different prion propensity of the two Ure2p homologs, this suggests that the detailed organization of the cross-? core may play an important role in the ..
  43. Scheidt V, Jüdes A, Bär C, Klassen R, Schaffrath R. Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling. Microb Cell. 2014;1:416-424 pubmed publisher
    ..Collectively, our data suggest that proper TOR signaling requires intact tRNA modifications and that loss of U34 modifications impinges on the TOR-sensitive NCR branch via Gln3 misregulation. ..
  44. Rubio Texeira M. Urmylation controls Nil1p and Gln3p-dependent expression of nitrogen-catabolite repressed genes in Saccharomyces cerevisiae. FEBS Lett. 2007;581:541-50 pubmed
    ..Altogether, the data presented here indicate an important role of the urmylation pathway in regulating the expression of genes involved in sensing and controlling amino acids levels. ..
  45. Fernandez Bellot E, Guillemet E, Baudin Baillieu A, Gaumer S, Komar A, Cullin C. Characterization of the interaction domains of Ure2p, a prion-like protein of yeast. Biochem J. 1999;338 ( Pt 2):403-7 pubmed
    ..A hypothesis has been put forward which states that [URE3] arises spontaneously from its cellular isoform Ure2p (the product of the URE2 gene), and propagates through interactions of the N-terminal domain of the protein, thus ..
  46. Baxa U, Cheng N, Winkler D, Chiu T, Davies D, Sharma D, et al. Filaments of the Ure2p prion protein have a cross-beta core structure. J Struct Biol. 2005;150:170-9 pubmed
    ..According to the "amyloid backbone" model, the N-terminal asparagine-rich domains of Ure2p polymerize to form an amyloid core fibril that is surrounded by C-terminal domains in their native conformation...
  47. Ngo S, Chiang V, Ho E, Le L, Guo Z. Prion domain of yeast Ure2 protein adopts a completely disordered structure: a solid-support EPR study. PLoS ONE. 2012;7:e47248 pubmed publisher
    ..Ure2 protein is the basis of yeast prion [URE3]. The Ure2p prion domain is largely disordered...
  48. Rai R, Cooper T. In vivo specificity of Ure2 protection from heavy metal ion and oxidative cellular damage in Saccharomyces cerevisiae. Yeast. 2005;22:343-58 pubmed
    The S. cerevisiae Ure2 protein is a prion precursor able to form large homopolymers with the characteristics of amyloid particles, a function largely restricted to its 90 N-terminal amino acids...
  49. Carvalho J, Zheng X. Domains of Gln3p interacting with karyopherins, Ure2p, and the target of rapamycin protein. J Biol Chem. 2003;278:16878-86 pubmed
    ..and sequestered in the cytoplasm in a manner that is dependent on the target of rapamycin (TOR) protein and Ure2p. In nonpreferred nitrogen or nitrogen starvation, Gln3p is dephosphorylated and imported into the nucleus via ..
  50. Martin O, Brandriss M, Schneider G, Bakalinsky A. Improved anaerobic use of arginine by Saccharomyces cerevisiae. Appl Environ Microbiol. 2003;69:1623-8 pubmed
    ..A pro3 ure2 strain expressing a PGK1 promoter-driven PUT2 allele encoding Delta(1)-pyrroline-5-carboxylate dehydrogenase ..
  51. Bousset L, Bonnefoy J, Sourigues Y, Wien F, Melki R. Structure and assembly properties of the N-terminal domain of the prion Ure2p in isolation and in its natural context. PLoS ONE. 2010;5:e9760 pubmed publisher
    The aggregation of the baker's yeast prion Ure2p is at the origin of the [URE3] trait...
  52. Xu L, Wu S, Buell A, Cohen S, Chen L, Hu W, et al. Influence of specific HSP70 domains on fibril formation of the yeast prion protein Ure2. Philos Trans R Soc Lond B Biol Sci. 2013;368:20110410 pubmed publisher
    b>Ure2p is the protein determinant of the Saccharomyces cerevisiae prion state [URE3]. Constitutive overexpression of the HSP70 family member SSA1 cures cells of [URE3]...
  53. Ungar L, Harari Y, Toren A, Kupiec M. Tor complex 1 controls telomere length by affecting the level of Ku. Curr Biol. 2011;21:2115-20 pubmed publisher
    ..The TORC1 signal is transduced by the Gln3/Gat1/Ure2 pathway, which controls the levels of the Ku heterodimer, a telomere regulator...
  54. Reineke L, Merrick W. Characterization of the functional role of nucleotides within the URE2 IRES element and the requirements for eIF2A-mediated repression. RNA. 2009;15:2264-77 pubmed publisher
    ..We have previously reported the secondary structure within the yeast minimal URE2 IRES element...
  55. Ripaud L, Maillet L, Immel Torterotot F, Durand F, Cullin C. The [URE3] yeast prion results from protein aggregates that differ from amyloid filaments formed in vitro. J Biol Chem. 2004;279:50962-8 pubmed
    The [URE3] yeast prion is a self-propagating inactive form of the Ure2 protein. Ure2p is composed of two domains, residues 1-93, the prion-forming domain, and the remaining C-terminal part of the protein, which forms the functional ..
  56. Makanae K, Kintaka R, Makino T, Kitano H, Moriya H. Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method. Genome Res. 2013;23:300-11 pubmed publisher
    ..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition. ..
  57. Reidy M, Sharma R, Roberts B, Masison D. Human J-protein DnaJB6b Cures a Subset of Saccharomyces cerevisiae Prions and Selectively Blocks Assembly of Structurally Related Amyloids. J Biol Chem. 2016;291:4035-47 pubmed publisher
    ..Yeast prions [URE3] and [PSI(+)] propagate as amyloid forms of Ure2 and Sup35 proteins, respectively...
  58. Xu S, Falvey D, Brandriss M. Roles of URE2 and GLN3 in the proline utilization pathway in Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:2321-30 pubmed
    ..The products of the GLN3 and URE2 genes are required for the appropriate transcription of many genes in alternative nitrogen assimilatory pathways...
  59. Kwan E, Foss E, Kruglyak L, Bedalov A. Natural polymorphism in BUL2 links cellular amino acid availability with chronological aging and telomere maintenance in yeast. PLoS Genet. 2011;7:e1002250 pubmed publisher
    ..Identification of a polymorphism in BUL2 in this outbred yeast population revealed a link among cellular amino acid availability, chronological lifespan, and telomere length control. ..
  60. Sabate R, Villar Piqué A, Espargaro A, Ventura S. Temperature dependence of the aggregation kinetics of Sup35 and Ure2p yeast prions. Biomacromolecules. 2012;13:474-83 pubmed publisher
    Fungal prions are protein-based genetic elements. Sup35 and Ure2p constitute the best-characterized prion proteins in the yeast Saccharomyces cerevisiae...
  61. Wickner R, Edskes H, Bateman D, Kelly A, Gorkovskiy A. The yeast prions [PSI+] and [URE3] are molecular degenerative diseases. Prion. 2011;5:258-62 pubmed publisher
    ..Recently, we showed that the array of [PSI] and [URE3] prions includes a majority of lethal or very toxic variants, a result not expected if either prion were an adaptive cellular response to stress. ..
  62. ter Schure E, Silljé H, Vermeulen E, Kalhorn J, Verkleij A, Boonstra J, et al. Repression of nitrogen catabolic genes by ammonia and glutamine in nitrogen-limited continuous cultures of Saccharomyces cerevisiae. Microbiology. 1998;144 ( Pt 5):1451-62 pubmed
    ..b>Ure2p has been shown to be an essential element for nitrogen-regulated gene expression...
  63. Kulkarni A, Abul Hamd A, Rai R, El Berry H, Cooper T. Gln3p nuclear localization and interaction with Ure2p in Saccharomyces cerevisiae. J Biol Chem. 2001;276:32136-44 pubmed
    ..b>Ure2p binds to Gln3p and Gat1p and is required for NCR-sensitive transcription to be repressed and for nuclear exclusion ..
  64. Lorenz M, Heitman J. Regulators of pseudohyphal differentiation in Saccharomyces cerevisiae identified through multicopy suppressor analysis in ammonium permease mutant strains. Genetics. 1998;150:1443-57 pubmed
    ..Overexpression of SRK1 (SSD1), URE2, DAL80, MEP1, or MEP3 suppressed only the growth defect of the Deltamep1/Deltamep1 Deltamep2/Deltamep2 mutant ..
  65. Dummer A, Su Z, Cherney R, Choi K, DENU J, Zhao X, et al. Binding of the Fkh1 Forkhead Associated Domain to a Phosphopeptide within the Mph1 DNA Helicase Regulates Mating-Type Switching in Budding Yeast. PLoS Genet. 2016;12:e1006094 pubmed publisher