Gene Symbol: URA1
Description: dihydroorotate dehydrogenase
Alias: dihydroorotate dehydrogenase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Jordan D, Bisaha J, Picollelli M. Catalytic properties of dihydroorotate dehydrogenase from Saccharomyces cerevisiae: studies on pH, alternate substrates, and inhibitors. Arch Biochem Biophys. 2000;378:84-92 pubmed
    Yeast dihydroorotate dehydrogenase (DHOD) was purified 2800-fold to homogeneity from its natural source. Its sequence is 70% identical to that of the Lactococcus lactis DHOD (family IA) and the two active sites are nearly the same...
  2. Vorisek J, Techniková Z, Schwippel J, Benoist P. Enzymatic activities of Ura2 and Ura1 proteins (aspartate carbamoyltransferase and dihydro-orotate dehydrogenase) are present in both isolated membranes and cytoplasm of Saccharomyces cerevisiae. Yeast. 2002;19:449-57 pubmed
    ..1.3.2). The fourth step of pyrimidine biosynthesis is catalysed by dihydro-orotate dehydrogenase (Ura1 protein; EC, which was similarly characterized as a peripheral membrane protein...
  3. Sprouse R, Shcherbakova I, Cheng H, Jamison E, Brenowitz M, Auble D. Function and structural organization of Mot1 bound to a natural target promoter. J Biol Chem. 2008;283:24935-48 pubmed publisher
    ..Using URA1 as a model Mot1-activated promoter, we show striking differences in the way that both TBP and Mot1 interact with ..
  4. Staresincic L, Walker J, Dirac Svejstrup A, Mitter R, Svejstrup J. GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein. J Biol Chem. 2011;286:35553-61 pubmed publisher
    ..Together, our data suggest that Npa3 defines an unconventional pathway for nuclear import of RNAPII, which involves GTP-dependent binding of Npa3 to the polymerase. ..
  5. Nagy M, Lacroute F, Thomas D. Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts. Proc Natl Acad Sci U S A. 1992;89:8966-70 pubmed
    A cDNA encoding the dihydroorotate dehydrogenase (DHOdehase; EC of the yeast Schizosaccharomyces pombe was isolated by functional complementation in Saccharomyces cerevisiae...
  6. Roy A. Nucleotide sequence of the URA1 gene of Saccharomyces cerevisiae. Gene. 1992;118:149-50 pubmed
    The complete nucleotide sequence of the URA1 gene encoding dihydroorotic acid dehydrogenase (DHOdehase) is presented...
  7. Jund R, Lacroute F. Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae. J Bacteriol. 1972;109:196-202 pubmed
    ..This enzyme appears, therefore, to escape the scheme of regulation by mixed repression and induction controlling the other enzymes of the pyrimidine pathway. ..
  8. Lacroute F. Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1968;95:824-32 pubmed
    ..Mutants that have simultaneously lost feedback inhibition by uridine triphosphate for carbamoyl phosphate synthetase and for aspartic transcarbamylase have been found and mapped in the gene ura-2. ..
  9. Dasgupta A, Juedes S, Sprouse R, Auble D. Mot1-mediated control of transcription complex assembly and activity. EMBO J. 2005;24:1717-29 pubmed
    ..For the Mot1-activated genes BNA1 and URA1, inactivation of Mot1 also leads, remarkably, to increased TBP binding to the promoters, despite the fact that ..

More Information


  1. Phan V, Ding V, Li F, Chalkley R, Burlingame A, McCormick F. The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans. Mol Cell Biol. 2010;30:2264-79 pubmed publisher
    ..These findings provide evidence for conserved ubiquitination pathways regulating the RasGAP proteins Ira2 (in yeast) and neurofibromin (in humans). ..