Gene Symbol: ULS1
Description: translocase ULS1
Alias: DIS1, RIS1, TID4, translocase ULS1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Uzunova K, Göttsche K, Miteva M, Weisshaar S, Glanemann C, Schnellhardt M, et al. Ubiquitin-dependent proteolytic control of SUMO conjugates. J Biol Chem. 2007;282:34167-75 pubmed
    ..was detected in Saccharomyces cerevisiae ubc4 ubc5 cells as well as in mutants lacking two RING finger proteins, Ris1 and Hex3/Slx5-Slx8, that bind to SUMO as well as to the ubiquitin-conjugating enzyme Ubc4...
  2. Meednu N, Hoops H, D Silva S, Pogorzala L, Wood S, Farkas D, et al. The spindle positioning protein Kar9p interacts with the sumoylation machinery in Saccharomyces cerevisiae. Genetics. 2008;180:2033-55 pubmed publisher
    ..We propose a model in which sumoylation regulates spindle positioning by restricting Kar9p to one SPB. These findings raise the possibility that sumoylation could regulate other microtubule-dependent processes. ..
  3. Shah P, Zheng X, Epshtein A, Carey J, Bishop D, Klein H. Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth. Mol Cell. 2010;39:862-72 pubmed publisher
    ..A triple mutant lacking Rdh54, Rad54, and a third Swi2/Snf2 homolog Uls1 accumulated Rad51 foci, grew slowly, and suffered chromosome loss...
  4. Zhang Z, Buchman A. Identification of a member of a DNA-dependent ATPase family that causes interference with silencing. Mol Cell Biol. 1997;17:5461-72 pubmed
    ..A previously uncharacterized gene, termed DIS1, was isolated from a screen for genes that interfere with silencing when overexpressed...
  5. Dresser M, Ewing D, Conrad M, Dominguez A, Barstead R, Jiang H, et al. DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics. 1997;147:533-44 pubmed
    ..Interestingly, the pattern on CHEF gels of chromosome fragments that result from meiotic DNA double-strand break formation is different in DMC1 mutant strains from that seen in rad50S strains. ..
  6. Kramarz K, Mucha S, Litwin I, Barg Wojas A, Wysocki R, Dziadkowiec D. DNA Damage Tolerance Pathway Choice Through Uls1 Modulation of Srs2 SUMOylation in Saccharomyces cerevisiae. Genetics. 2017;206:513-525 pubmed publisher
    ..Here we show that Saccharomyces cerevisiae Uls1, a member of the Swi2/Snf2 family of ATPases and a SUMO-targeted ubiquitin ligase, physically interacts with both ..
  7. Psakhye I, Jentsch S. Protein group modification and synergy in the SUMO pathway as exemplified in DNA repair. Cell. 2012;151:807-820 pubmed publisher
    ..We propose that SUMOylation may thus often target a protein group rather than individual proteins, whereas localized modification enzymes and highly specific triggers ensure specificity. ..
  8. Cal Bakowska M, Litwin I, Bocer T, Wysocki R, Dziadkowiec D. The Swi2-Snf2-like protein Uls1 is involved in replication stress response. Nucleic Acids Res. 2011;39:8765-77 pubmed publisher
    The Saccharomyces cerevisiae Uls1 belongs to the Swi2-Snf2 family of DNA-dependent ATPases and a new protein family of SUMO-targeted ubiquitin ligases...
  9. Schweiggert J, Stevermann L, Panigada D, Kammerer D, Liakopoulos D. Regulation of a Spindle Positioning Factor at Kinetochores by SUMO-Targeted Ubiquitin Ligases. Dev Cell. 2016;36:415-27 pubmed publisher
    ..We propose that kinetochores serve as platforms to recruit STUbLs in a SUMO-dependent manner in order to ensure correct spindle function by regulating levels of microtubule-associated proteins. ..

More Information


  1. Shirai C, Mizuta K. SUMO mediates interaction of Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, with a RING finger protein Ris1p. Biosci Biotechnol Biochem. 2008;72:1881-6 pubmed
    ..A SUMO-binding motif of Ris1p was required for interaction with Ebp2p. These results suggest that SUMO mediates the interaction between Ebp2p and SUMO related proteins and that Ebp2p switches its interaction partners via sumoylation. ..
  2. Parnas O, Amishay R, Liefshitz B, Zipin Roitman A, Kupiec M. Elg1, the major subunit of an alternative RFC complex, interacts with SUMO-processing proteins. Cell Cycle. 2011;10:2894-903 pubmed
    ..Thus our results highlight the many important roles played by Elg1, some of which are PCNA-dependent and some PCNA-independent. ..
  3. Tan W, Wang Z, Prelich G. Physical and Genetic Interactions Between Uls1 and the Slx5-Slx8 SUMO-Targeted Ubiquitin Ligase. G3 (Bethesda). 2013;3:771-780 pubmed publisher
    ..pathways, a screen was performed for high-copy suppressors of mot1-301, yielding three genes (MOT3, MIT1, and ULS1)...
  4. Alonso A, D Silva S, Rahman M, Meluh P, Keeling J, Meednu N, et al. The yeast homologue of the microtubule-associated protein Lis1 interacts with the sumoylation machinery and a SUMO-targeted ubiquitin ligase. Mol Biol Cell. 2012;23:4552-66 pubmed publisher
    ..Both Pac1p and Bik1p interact with the STUbL Nis1p-Ris1p and the protease Wss1p. Strains deleted for RIS1 or WSS1 accumulate Pac1p conjugates...
  5. Kramarz K, Litwin I, Cal Bakowska M, Szakal B, Branzei D, Wysocki R, et al. Swi2/Snf2-like protein Uls1 functions in the Sgs1-dependent pathway of maintenance of rDNA stability and alleviation of replication stress. DNA Repair (Amst). 2014;21:24-35 pubmed publisher
    The Saccharomyces cerevisiae Uls1 belongs to the Swi2/Snf2 family of DNA-dependent ATPases and a new protein family of SUMO-targeted ubiquitin ligases...
  6. Chi P, Kwon Y, Visnapuu M, Lam I, Santa Maria S, Zheng X, et al. Analyses of the yeast Rad51 recombinase A265V mutant reveal different in vivo roles of Swi2-like factors. Nucleic Acids Res. 2011;39:6511-22 pubmed publisher
    ..Additionally, we show that a third Swi2-like factor, Uls1, contributes toward Rad51 clearance from chromatin in the absence of Rad54 and Rdh54, and define a hierarchy of ..
  7. Lescasse R, Pobiega S, Callebaut I, Marcand S. End-joining inhibition at telomeres requires the translocase and polySUMO-dependent ubiquitin ligase Uls1. EMBO J. 2013;32:805-15 pubmed publisher
    ..In budding yeast, binding of Rap1 to telomere repeats establishes NHEJ inhibition. Here, we show that the Uls1 protein is required for the maintenance of NHEJ inhibition at telomeres...
  8. Tosti E, Katakowski J, Schaetzlein S, Kim H, Ryan C, Shales M, et al. Evolutionarily conserved genetic interactions with budding and fission yeast MutS identify orthologous relationships in mismatch repair-deficient cancer cells. Genome Med. 2014;6:68 pubmed publisher
    ..Moreover, we provide novel insights into the genome maintenance functions of a critical DNA repair complex and propose a promising targeted treatment for MMR deficient tumors. ..