Gene Symbol: UBP2
Description: ubiquitin-specific protease UBP2
Alias: ubiquitin-specific protease UBP2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Baker R, Tobias J, Varshavsky A. Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family. J Biol Chem. 1992;267:23364-75 pubmed
    ..coli-based genetic screen, we have isolated two other yeast genes for ubiquitin-specific proteases, named UBP2 and UBP3...
  2. Ren J, Kee Y, Huibregtse J, Piper R. Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies. Mol Biol Cell. 2007;18:324-35 pubmed
    ..a PY element within its C-terminus and through a novel protein Hua1, which recruits a complex of Rsp5, Rup1, and Ubp2. The SH3 domain of Hse1 also binds to the deubiquitinating protein Ubp7...
  3. Harreman M, Taschner M, Sigurdsson S, Anindya R, Reid J, Somesh B, et al. Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation. Proc Natl Acad Sci U S A. 2009;106:20705-10 pubmed publisher
    ..When produced, the K63 chain can be trimmed to mono-ubiquitylation by an Rsp5-associated ubiquitin protease, Ubp2. Based on this mono-ubiquitin moiety on RNAPII, an Elc1/Cul3 complex then produces a ubiquitin chain linked via ..
  4. Kee Y, Lyon N, Huibregtse J. The Rsp5 ubiquitin ligase is coupled to and antagonized by the Ubp2 deubiquitinating enzyme. EMBO J. 2005;24:2414-24 pubmed
    ..b>Ubp2, a deubiquitinating enzyme, was a prominent copurifying protein...
  5. Kee Y, Muñoz W, Lyon N, Huibregtse J. The deubiquitinating enzyme Ubp2 modulates Rsp5-dependent Lys63-linked polyubiquitin conjugates in Saccharomyces cerevisiae. J Biol Chem. 2006;281:36724-31 pubmed
    ..to E6AP C terminus) ubiquitin ligase involved in numerous processes, and an associated deubiquitinating enzyme, Ubp2, modulates its activity. A dramatic increase in Lys(63)-linked conjugates was observed in ubp2Delta cells...
  6. Lam M, Urban Grimal D, Bugnicourt A, Greenblatt J, Haguenauer Tsapis R, Emili A. Interaction of the deubiquitinating enzyme Ubp2 and the e3 ligase Rsp5 is required for transporter/receptor sorting in the multivesicular body pathway. PLoS ONE. 2009;4:e4259 pubmed publisher
    ..We sought to elucidate the possible involvement of the S. cerevisiae deubiquitinating enzyme Ubp2 in transporter and receptor trafficking after we (this study) and others established that affinity purified Ubp2 ..
  7. Schaefer J, Morgan D. Protein-linked ubiquitin chain structure restricts activity of deubiquitinating enzymes. J Biol Chem. 2011;286:45186-96 pubmed publisher
    ..We propose that the resistance to many DUBs of long, substrate-attached Lys(48)-linked chains helps ensure that proteins are maintained free from ubiquitin until a threshold of ubiquitin ligase activity enables degradation...
  8. Anton F, Dittmar G, Langer T, Escobar Henriques M. Two deubiquitylases act on mitofusin and regulate mitochondrial fusion along independent pathways. Mol Cell. 2013;49:487-98 pubmed publisher
    ..mitofusin Fzo1 is regulated by sequential ubiquitylation at conserved lysine residues and by the deubiquitylases Ubp2 and Ubp12...
  9. Silva G, Finley D, Vogel C. K63 polyubiquitination is a new modulator of the oxidative stress response. Nat Struct Mol Biol. 2015;22:116-23 pubmed publisher
    ..We demonstrate that hydrogen peroxide inhibits the deubiquitinating enzyme Ubp2, leading to accumulation of K63 conjugates assembled by the Rad6 ubiquitin conjugase and the Bre1 ubiquitin ligase...

More Information


  1. Winkler A, Korstanje R, Zonneveld B, Hooykaas P, Steensma H. Isolation and characterization of KIUBP2, a ubiquitin hydrolase gene of Kluyveromyces lactis that can suppress a ts-mutation in CBF2, a gene encoding a centromeric protein of Saccharomyces cerevisiae. Curr Genet. 2000;38:17-22 pubmed
    The Kluyveromyces lactis UBP2 gene was isolated as a suppressor of a temperature-sensitive mutation in CBF2, a gene coding for a centromere-binding protein of Saccharomyces cerevisiae...
  2. Lam M, Emili A. Ubp2 regulates Rsp5 ubiquitination activity in vivo and in vitro. PLoS ONE. 2013;8:e75372 pubmed publisher
    ..we and others [1], [2] reported the physical and functional interaction of Rsp5 with the deubiquitinating enzyme Ubp2, and the ubiquitin associated (UBA) domain-containing cofactor Rup1...
  3. Ho H, MacGurn J, Emr S. Deubiquitinating enzymes Ubp2 and Ubp15 regulate endocytosis by limiting ubiquitination and degradation of ARTs. Mol Biol Cell. 2017;28:1271-1283 pubmed publisher
    ..Here we report that the stability of ARTs is regulated by the deubiquitinating enzymes (DUBs) Ubp2 and Ubp15...
  4. Layfield R, Franklin K, Landon M, Walker G, Wang P, Ramage R, et al. Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes. Anal Biochem. 1999;274:40-9 pubmed
    ..This work demonstrates that the synthetic approach offers an alternative to recombinant methods for the production of small proteins in vitro. ..