UBI4

Summary

Gene Symbol: UBI4
Description: ubiquitin
Alias: SCD2, UB14, ubiquitin
Species: Saccharomyces cerevisiae S288c
Products:     UBI4

Top Publications

  1. Stawiecka Mirota M, Pokrzywa W, Morvan J, Zoladek T, Haguenauer Tsapis R, Urban Grimal D, et al. Targeting of Sna3p to the endosomal pathway depends on its interaction with Rsp5p and multivesicular body sorting on its ubiquitylation. Traffic. 2007;8:1280-96 pubmed
    Rsp5p is an ubiquitin (Ub)-protein ligase of the Nedd4 family that carries WW domains involved in interaction with PPXY-containing proteins...
  2. Hoege C, Pfander B, Moldovan G, Pyrowolakis G, Jentsch S. RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO. Nature. 2002;419:135-41 pubmed
    ..Two principal elements of this pathway are the ubiquitin-conjugating enzymes RAD6 and the MMS2-UBC13 heterodimer, which are recruited to chromatin by the RING-finger ..
  3. Ozkaynak E, Finley D, Solomon M, Varshavsky A. The yeast ubiquitin genes: a family of natural gene fusions. EMBO J. 1987;6:1429-39 pubmed
    b>Ubiquitin is a 76-residue protein highly conserved among eukaryotes. Conjugation of ubiquitin to intracellular proteins mediates their selective degradation in vivo...
  4. Metzger M, Liang Y, Das R, Mariano J, Li S, Li J, et al. A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms. Mol Cell. 2013;50:516-27 pubmed publisher
    Cue1p is an integral component of yeast endoplasmic reticulum (ER)-associated degradation (ERAD) ubiquitin ligase (E3) complexes...
  5. Shih S, Prag G, Francis S, Sutanto M, Hurley J, Hicke L. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003;22:1273-81 pubmed
    ..Monoubiquitin signals are likely to be recognized by ubiquitin-binding proteins that transmit the regulatory information conferred by monoubiquitylation...
  6. Zoladek T, Tobiasz A, Vaduva G, Boguta M, Martin N, Hopper A. MDP1, a Saccharomyces cerevisiae gene involved in mitochondrial/cytoplasmic protein distribution, is identical to the ubiquitin-protein ligase gene RSP5. Genetics. 1997;145:595-603 pubmed
    ..MDP1 is identical to RSP5, which encodes ubiquitin-protein ligase, and mdp1 mutations are suppressed by high copy expression of ubiquitin...
  7. Iglesias N, Tutucci E, Gwizdek C, Vinciguerra P, Von Dach E, Corbett A, et al. Ubiquitin-mediated mRNP dynamics and surveillance prior to budding yeast mRNA export. Genes Dev. 2010;24:1927-38 pubmed publisher
  8. Springael J, Andre B. Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:1253-63 pubmed
    ..inactivation and degradation of the general amino acid permease Gap1 through a process requiring the Npi1/Rsp5 ubiquitin (Ub) ligase...
  9. Scott P, Bilodeau P, Zhdankina O, Winistorfer S, Hauglund M, Allaman M, et al. GGA proteins bind ubiquitin to facilitate sorting at the trans-Golgi network. Nat Cell Biol. 2004;6:252-9 pubmed
    ..b>Ubiquitin may also mediate sorting of proteins from the trans-Golgi network (TGN) to the endosome, thereby preventing ..

More Information

Publications95

  1. Strochlic T, Schmiedekamp B, Lee J, Katzmann D, Burd C. Opposing activities of the Snx3-retromer complex and ESCRT proteins mediate regulated cargo sorting at a common endosome. Mol Biol Cell. 2008;19:4694-706 pubmed publisher
    ..In mutant cells lacking any component of the ESCRT protein-dependent MVB sorting machinery, the Rsp5 ubiquitin ligase, or in wild-type cells expressing Fet3-Ftr1 lacking cytosolic lysyl ubiquitin acceptor sites, Fet3-Ftr1 ..
  2. Platta H, Grunau S, Rosenkranz K, Girzalsky W, Erdmann R. Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol. Nat Cell Biol. 2005;7:817-22 pubmed
    ..of the receptors has been assigned to peroxins including the AAA peroxins Pex1p and Pex6p, as well as the ubiquitin-conjugating enzyme Pex4p (reviewed in ref. 2)...
  3. Bilodeau P, Winistorfer S, Kearney W, Robertson A, Piper R. Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome. J Cell Biol. 2003;163:237-43 pubmed
    b>Ubiquitin (Ub) attachment to cell surface proteins causes their lysosomal degradation by incorporating them into lumenal membranes of multivesicular bodies (MVBs)...
  4. Zhang D, Chen T, Ziv I, Rosenzweig R, Matiuhin Y, Bronner V, et al. Together, Rpn10 and Dsk2 can serve as a polyubiquitin chain-length sensor. Mol Cell. 2009;36:1018-33 pubmed publisher
    ..We obtained structural information on two receptors, Rpn10 and Dsk2, alone and in complex with (poly)ubiquitin or with each other...
  5. Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, et al. A high throughput screen to identify substrates for the ubiquitin ligase Rsp5. J Biol Chem. 2005;280:29470-8 pubmed
    b>Ubiquitin-protein ligases (E3s) are implicated in various human disorders and are attractive targets for therapeutic intervention...
  6. Fujii K, Kitabatake M, Sakata T, Ohno M. 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay. EMBO J. 2012;31:2579-89 pubmed publisher
    ..We have previously reported that 25S NRD requires an E3 ubiquitin ligase complex, which is involved in ribosomal ubiquitination...
  7. Ren J, Kee Y, Huibregtse J, Piper R. Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies. Mol Biol Cell. 2007;18:324-35 pubmed
    ..We show that the endosomal Hse1-Vps27 sorting receptor binds to ubiquitin peptidases and the ubiquitin ligase Rsp5...
  8. Raasi S, Varadan R, Fushman D, Pickart C. Diverse polyubiquitin interaction properties of ubiquitin-associated domains. Nat Struct Mol Biol. 2005;12:708-14 pubmed
    The ubiquitin-associated (UBA) domain occurs frequently in proteins involved in ubiquitin-dependent signaling pathways...
  9. Reggiori F, Pelham H. A transmembrane ubiquitin ligase required to sort membrane proteins into multivesicular bodies. Nat Cell Biol. 2002;4:117-23 pubmed
    ..We have identified a transmembrane ubiquitin ligase, Tul1, that is resident in the Golgi apparatus and is required for the ubiquitination of proteins with ..
  10. Zhou P, Howley P. Ubiquitination and degradation of the substrate recognition subunits of SCF ubiquitin-protein ligases. Mol Cell. 1998;2:571-80 pubmed
    The S. cerevisiae SCFCdc4p ubiquitin-protein ligase complex promotes cell cycle transitions through degradation of cell cycle regulators...
  11. Yashiroda H, Oguchi T, Yasuda Y, Toh e A, Kikuchi Y. Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:3255-63 pubmed
    ..reverses Spt- phenotype), which encodes a hect (homologous to E6-AP C terminus) domain, as a gene encoding a ubiquitin ligase. Thus, we named our gene BUL1 (for a protein that binds to the ubiquitin ligase)...
  12. Kim H, Huibregtse J. Polyubiquitination by HECT E3s and the determinants of chain type specificity. Mol Cell Biol. 2009;29:3307-18 pubmed publisher
    Polyubiquitination can mediate several different biochemical functions, determined in part by which lysine of ubiquitin is used to link the polyubiquitin chain...
  13. Chen P, Johnson P, Sommer T, Jentsch S, Hochstrasser M. Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor. Cell. 1993;74:357-69 pubmed
    Attachment of ubiquitin to proteins is catalyzed by a family of ubiquitin-conjugating (UBC) enzymes...
  14. Koegl M, Hoppe T, Schlenker S, Ulrich H, Mayer T, Jentsch S. A novel ubiquitination factor, E4, is involved in multiubiquitin chain assembly. Cell. 1999;96:635-44 pubmed
    ..Ubiquitination involves a ubiquitin-activating enzyme, E1, a ubiquitin-conjugating enzyme, E2, and often a substrate-specific ubiquitin-protein ..
  15. Mayor T, Lipford J, Graumann J, Smith G, Deshaies R. Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets. Mol Cell Proteomics. 2005;4:741-51 pubmed
    ..In contrast, turnover of at least one ubiquitin-proteasome system (UPS) substrate, CPY*, is impervious to deletion of RPN10...
  16. Skowyra D, Craig K, Tyers M, Elledge S, Harper J. F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell. 1997;91:209-19 pubmed
    ..Skp1, Cdc53, and the F-box protein Cdc4 form a complex, SCFCdc4, which functions as a Sic1 ubiquitin-ligase (E3) in combination with the ubiquitin conjugating enzyme (E2) Cdc34 and E1...
  17. Radivojac P, Vacic V, Haynes C, Cocklin R, Mohan A, Heyen J, et al. Identification, analysis, and prediction of protein ubiquitination sites. Proteins. 2010;78:365-80 pubmed publisher
    ..of ubiquitination sites is challenging due to rapid turnover of ubiquitinated proteins and the large size of the ubiquitin modifier...
  18. Davies A, Huttner D, Daigaku Y, Chen S, Ulrich H. Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a. Mol Cell. 2008;29:625-36 pubmed publisher
    ..We found that RPA directly interacts with the ubiquitin ligase responsible for the modification of PCNA, Rad18, both in yeast and in mammalian cells...
  19. Finley D, Sadis S, Monia B, Boucher P, Ecker D, Crooke S, et al. Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. Mol Cell Biol. 1994;14:5501-9 pubmed
    The degradation of many proteins requires their prior attachment to ubiquitin. Proteolytic substrates are characteristically multiubiquitinated through the formation of ubiquitin-ubiquitin linkages...
  20. van Nocker S, Sadis S, Rubin D, Glickman M, Fu H, Coux O, et al. The multiubiquitin-chain-binding protein Mcb1 is a component of the 26S proteasome in Saccharomyces cerevisiae and plays a nonessential, substrate-specific role in protein turnover. Mol Cell Biol. 1996;16:6020-8 pubmed
    The 26S proteasome is an essential proteolytic complex that is responsible for degrading proteins conjugated with ubiquitin. It has been proposed that the recognition of substrates by the 26S proteasome is mediated by a multiubiquitin-..
  21. Swaminathan S, Amerik A, Hochstrasser M. The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeast. Mol Biol Cell. 1999;10:2583-94 pubmed
    Attachment of ubiquitin to cellular proteins frequently targets them to the 26S proteasome for degradation...
  22. Bertolaet B, Clarke D, Wolff M, Watson M, Henze M, Divita G, et al. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nat Struct Biol. 2001;8:417-22 pubmed
    ..Its C-terminal ubiquitin-associated (UBA) domain is evolutionarily conserved from yeast to humans...
  23. Rodrigo Brenni M, Morgan D. Sequential E2s drive polyubiquitin chain assembly on APC targets. Cell. 2007;130:127-39 pubmed
    The anaphase-promoting complex (APC), or cyclosome, is an E3 ubiquitin-protein ligase that collaborates with E2 ubiquitin-conjugating enzymes to assemble polyubiquitin chains on proteins important for cell-cycle progression...
  24. Michel J, McCarville J, Xiong Y. A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression. J Biol Chem. 2003;278:22828-37 pubmed
    ..We demonstrate that these cullins are associated in vivo with ubiquitin ligase activity...
  25. Ju D, Wang X, Xu H, Xie Y. Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation. Mol Cell Biol. 2008;28:1404-12 pubmed
    ..mutants generated by the Saccharomyces Genome Deletion Project and identified the mub1Delta mutant defective in ubiquitin-dependent degradation of Rpn4...
  26. Jarmoszewicz K, Łukasiak K, Riezman H, Kaminska J. Rsp5 ubiquitin ligase is required for protein trafficking in Saccharomyces cerevisiae COPI mutants. PLoS ONE. 2012;7:e39582 pubmed publisher
    ..With the exception of the Sec27 protein (?'COP) neither the ubiquitin ligase responsible for ubiquitination of COPI subunits nor the importance of this modification are known...
  27. Merhi A, Andre B. Internal amino acids promote Gap1 permease ubiquitylation via TORC1/Npr1/14-3-3-dependent control of the Bul arrestin-like adaptors. Mol Cell Biol. 2012;32:4510-22 pubmed publisher
    ..This ubiquitylation requires the Rsp5 ubiquitin ligase and the redundant arrestin-like Bul1 and Bul2 adaptors...
  28. Deng Y, Guo Y, Watson H, Au W, Shakoury Elizeh M, Basrai M, et al. Gga2 mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1 from the trans-Golgi network to the vacuole. J Biol Chem. 2009;284:23830-41 pubmed publisher
    ..Although Ggas have been characterized as ubiquitin receptors, we show here that ubiquitin binding by Gga2 was not required for the TGN-to-endosome trafficking of ..
  29. French M, Kretzmann B, Hicke L. Regulation of the RSP5 ubiquitin ligase by an intrinsic ubiquitin-binding site. J Biol Chem. 2009;284:12071-9 pubmed publisher
    Rsp5 is a homologous to E6AP C terminus (HECT) ubiquitin ligase (E3) that controls many different cellular processes in budding yeast...
  30. Schaefer J, Morgan D. Protein-linked ubiquitin chain structure restricts activity of deubiquitinating enzymes. J Biol Chem. 2011;286:45186-96 pubmed publisher
    ..This resistance is lost if the structure of Lys(48)-linked chains is disrupted by mutation of ubiquitin or if chains are linked through Lys(63)...
  31. Peng J, Schwartz D, Elias J, Thoreen C, Cheng D, Marsischky G, et al. A proteomics approach to understanding protein ubiquitination. Nat Biotechnol. 2003;21:921-6 pubmed
    ..We report here a proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate...
  32. Parker J, Ulrich H. Mechanistic analysis of PCNA poly-ubiquitylation by the ubiquitin protein ligases Rad18 and Rad5. EMBO J. 2009;28:3657-66 pubmed publisher
    ..Several distinct mechanisms have been reported for the assembly of poly-ubiquitin chains, involving either stepwise transfer of ubiquitin monomers or attachment of a preformed poly-ubiquitin ..
  33. Krsmanovic T, Kölling R. The HECT E3 ubiquitin ligase Rsp5 is important for ubiquitin homeostasis in yeast. FEBS Lett. 2004;577:215-9 pubmed
    The HECT E3 ubiquitin ligase Rsp5, a yeast member of the Nedd4 family, has been implicated in many different aspects of cell physiology. Here, we present evidence that Rsp5 function is important for ubiquitin homeostasis...
  34. Berndsen C, Wolberger C. A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Anal Biochem. 2011;418:102-10 pubmed publisher
    ..b>Ubiquitin is conjugated to a substrate lysine in an enzymatic cascade involving an E1 ubiquitin-activating enzyme, an E2 ..
  35. Stimpson H, Lewis M, Pelham H. Transferrin receptor-like proteins control the degradation of a yeast metal transporter. EMBO J. 2006;25:662-72 pubmed
    ..Degradation requires Bsd2, a membrane protein with a PPxY motif that recruits the ubiquitin ligase Rsp5, and which has a role in the quality control of membrane proteins, that expose hydrophilic residues ..
  36. Verma R, Oania R, Fang R, Smith G, Deshaies R. Cdc48/p97 mediates UV-dependent turnover of RNA Pol II. Mol Cell. 2011;41:82-92 pubmed publisher
    Cdc48/p97 is an essential ATPase whose role in targeting substrates to the ubiquitin-proteasome system (UPS) remains unclear. Existing models posit that Cdc48 acts upstream of UPS receptors...
  37. Ribar B, Prakash L, Prakash S. ELA1 and CUL3 are required along with ELC1 for RNA polymerase II polyubiquitylation and degradation in DNA-damaged yeast cells. Mol Cell Biol. 2007;27:3211-6 pubmed
    ..However, the ubiquitin ligase (E3) responsible for Pol II polyubiquitylation has not been identified in humans or the yeast ..
  38. Chernova T, Allen K, Wesoloski L, Shanks J, Chernoff Y, Wilkinson K. Pleiotropic effects of Ubp6 loss on drug sensitivities and yeast prion are due to depletion of the free ubiquitin pool. J Biol Chem. 2003;278:52102-15 pubmed
    ..Conversely, overexpression of ubiquitin (Ub) increases phenotypic expression and induction of [PSI+] in the wild type cells and suppresses all tested ..
  39. Nakatsukasa K, Huyer G, Michaelis S, Brodsky J. Dissecting the ER-associated degradation of a misfolded polytopic membrane protein. Cell. 2008;132:101-12 pubmed publisher
    ..We found that specific Hsp70/40s act before ubiquitination and facilitate Ste6p(*) association with an E3 ubiquitin ligase, suggesting an active role for chaperones...
  40. Emre N, Ingvarsdottir K, Wyce A, Wood A, Krogan N, Henry K, et al. Maintenance of low histone ubiquitylation by Ubp10 correlates with telomere-proximal Sir2 association and gene silencing. Mol Cell. 2005;17:585-94 pubmed
    ..Here, we show that the ubiquitin protease Ubp10 targets H2B for deubiquitylation, helping to localize Sir2 to the telomere...
  41. Rumpf S, Jentsch S. Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone. Mol Cell. 2006;21:261-9 pubmed
    b>Ubiquitin-dependent protein degradation usually involves escort factors that target ubiquitylated substrates to the proteasome...
  42. El Magraoui F, Brinkmeier R, Schrötter A, Girzalsky W, Müller T, Marcus K, et al. Distinct ubiquitination cascades act on the peroxisomal targeting signal type 2 co-receptor Pex18p. Traffic. 2013;14:1290-301 pubmed publisher
    ..Using in vivo and in vitro approaches, we demonstrate that the polyubiquitination of Pex18p requires the ubiquitin-conjugating enzyme (E2) Ubc4p, which cooperates with the RING (really interesting new gene)-type ubiquitin-..
  43. Saeki Y, Kudo T, Sone T, Kikuchi Y, Yokosawa H, Toh e A, et al. Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome. EMBO J. 2009;28:359-71 pubmed publisher
    ..In contrast, modifications with the Lys63-linked polyubiquitin chain and/or monomeric ubiquitin are generally thought to function in proteasome-independent cellular processes...
  44. Parker J, Bielen A, Dikic I, Ulrich H. Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase eta in Saccharomyces cerevisiae. Nucleic Acids Res. 2007;35:881-9 pubmed
    ..We have analyzed the contributions of ubiquitin and PCNA binding to damage bypass and damage-induced mutagenesis in Polymerase eta (encoded by RAD30) from the ..
  45. Kim Y, Deng Y, Philpott C. GGA2- and ubiquitin-dependent trafficking of Arn1, the ferrichrome transporter of Saccharomyces cerevisiae. Mol Biol Cell. 2007;18:1790-802 pubmed
    ..Here, we report that the clathrin adaptor Gga2 and ubiquitination by the Rsp5 ubiquitin ligase are required for trafficking of Arn1...
  46. Ravid T, Hochstrasser M. Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue. Nat Cell Biol. 2007;9:422-7 pubmed
    ..b>Ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s) work together to assemble polyubiquitin chains on ..
  47. Somesh B, Sigurdsson S, Saeki H, Erdjument Bromage H, Tempst P, Svejstrup J. Communication between distant sites in RNA polymerase II through ubiquitylation factors and the polymerase CTD. Cell. 2007;129:57-68 pubmed
    ..These data reveal the specificity and mechanism of RNAPII ubiquitylation and demonstrate that E2s can play a crucial role in substrate recognition. ..
  48. Beaudenon S, Huacani M, Wang G, McDonnell D, Huibregtse J. Rsp5 ubiquitin-protein ligase mediates DNA damage-induced degradation of the large subunit of RNA polymerase II in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:6972-9 pubmed
    Rsp5 is an E3 ubiquitin-protein ligase of Saccharomyces cerevisiae that belongs to the hect domain family of E3 proteins. We have previously shown that Rsp5 binds and ubiquitinates the largest subunit of RNA polymerase II, Rpb1, in vitro...
  49. Rao H, Sastry A. Recognition of specific ubiquitin conjugates is important for the proteolytic functions of the ubiquitin-associated domain proteins Dsk2 and Rad23. J Biol Chem. 2002;277:11691-5 pubmed
    b>Ubiquitin (Ub) regulates important cellular processes through covalent attachment to its substrates. The fate of a substrate depends on the number of ubiquitin moieties conjugated, as well as the lysine linkage of Ub-Ub conjugation...
  50. Shih S, Katzmann D, Schnell J, Sutanto M, Emr S, Hicke L. Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis. Nat Cell Biol. 2002;4:389-93 pubmed
    b>Ubiquitin functions as a signal for sorting cargo at multiple steps of the endocytic pathway and controls the activity of trans-acting components of the endocytic machinery (reviewed in refs 1, and 2)...
  51. Leon S, Erpapazoglou Z, Haguenauer Tsapis R. Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies. Mol Biol Cell. 2008;19:2379-88 pubmed publisher
    ..In yeast, this process requires Rsp5p, an essential ubiquitin ligase of the Nedd4 family...
  52. Funakoshi M, Sasaki T, Nishimoto T, Kobayashi H. Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proc Natl Acad Sci U S A. 2002;99:745-50 pubmed
    Dsk2p from Saccharomyces cerevisiae belongs to the class of proteins that contain a ubiquitin-like (UbL) domain at the N terminus together with a ubiquitin-associated (UBA) domain at the C terminus...
  53. Fang N, Ng A, Measday V, Mayor T. Hul5 HECT ubiquitin ligase plays a major role in the ubiquitylation and turnover of cytosolic misfolded proteins. Nat Cell Biol. 2011;13:1344-52 pubmed publisher
    ..Several protein quality control mechanisms degrade non-native proteins by the ubiquitin-proteasome system...
  54. Dores M, Schnell J, Maldonado Baez L, Wendland B, Hicke L. The function of yeast epsin and Ede1 ubiquitin-binding domains during receptor internalization. Traffic. 2010;11:151-60 pubmed publisher
    ..Epsins and Eps15-like proteins are ubiquitin-binding proteins that act early in this process...
  55. Hensel A, Beck S, El Magraoui F, Platta H, Girzalsky W, Erdmann R. Cysteine-dependent ubiquitination of Pex18p is linked to cargo translocation across the peroxisomal membrane. J Biol Chem. 2011;286:43495-505 pubmed publisher
    ..Here we demonstrate that Pex18p undergoes two kinds of ubiquitin modifications...
  56. Lee J, Oestreich A, Payne J, Gunawan M, Norgan A, Katzmann D. The HECT domain of the ubiquitin ligase Rsp5 contributes to substrate recognition. J Biol Chem. 2009;284:32126-37 pubmed publisher
    b>Ubiquitin modification of endosomal membrane proteins is a signal for active inclusion into the Multivesicular Body (MVB) pathway, resulting in lysosomal degradation...
  57. van der Kemp P, de Padula M, Burguiere Slezak G, Ulrich H, Boiteux S. PCNA monoubiquitylation and DNA polymerase eta ubiquitin-binding domain are required to prevent 8-oxoguanine-induced mutagenesis in Saccharomyces cerevisiae. Nucleic Acids Res. 2009;37:2549-59 pubmed publisher
    ..We also report that the ubiquitin-binding domain (UBZ) of Pol eta is essential to prevent 8-oxoG-induced mutagenesis but only in conjunction with ..
  58. Boban M, Pantazopoulou M, Schick A, Ljungdahl P, Foisner R. A nuclear ubiquitin-proteasome pathway targets the inner nuclear membrane protein Asi2 for degradation. J Cell Sci. 2014;127:3603-13 pubmed publisher
    ..Asi2 exhibits enhanced stability in mutants lacking the E2 ubiquitin conjugating enzymes Ubc6 or Ubc7, or the E3 ubiquitin ligase Doa10...
  59. Pizzirusso M, Chang A. Ubiquitin-mediated targeting of a mutant plasma membrane ATPase, Pma1-7, to the endosomal/vacuolar system in yeast. Mol Biol Cell. 2004;15:2401-9 pubmed
    ..Ubiquitination and endosomal targeting of Pma1-7 is dependent on the Rsp5-Bul1-Bul2 ubiquitin ligase protein complex but not the transmembrane ubiquitin ligase Tul1...
  60. Richter C, West M, Odorizzi G. Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies. EMBO J. 2007;26:2454-64 pubmed
    Doa4 is a ubiquitin-specific protease in Saccharomyces cerevisiae that deubiquitinates integral membrane proteins sorted into the lumenal vesicles of late-endosomal multivesicular bodies (MVBs)...
  61. Hochstrasser M. Ubiquitin-dependent protein degradation. Annu Rev Genet. 1996;30:405-39 pubmed
    A growing number of cellular regulatory mechanisms are being linked to protein modification by the polypeptide ubiquitin. These include key transitions in the cell cycle, class I antigen processing, signal transduction pathways, and ..
  62. Wilson M, Harreman M, Taschner M, Reid J, Walker J, Erdjument Bromage H, et al. Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress. Cell. 2013;154:983-995 pubmed publisher
    ..Nuclear Def1 then binds RNAPII, utilizing a ubiquitin-binding domain to recruit the Elongin-Cullin E3 ligase complex via a ubiquitin-homology domain in the Ela1 ..
  63. Galan J, Haguenauer Tsapis R. Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein. EMBO J. 1997;16:5847-54 pubmed
    ..yeast plasma membrane uracil permease undergoes cell-surface ubiquitination, which is dependent on the Npi1/Rsp5 ubiquitin-protein ligase...
  64. Bays N, Gardner R, Seelig L, Joazeiro C, Hampton R. Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation. Nat Cell Biol. 2001;3:24-9 pubmed
    ..ERAD proceeds by the ubiquitin-proteasome pathway, in which the covalent attachment of ubiquitin to proteins targets them for proteasomal ..
  65. Hitchcock A, Auld K, Gygi S, Silver P. A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. Proc Natl Acad Sci U S A. 2003;100:12735-40 pubmed
    ..Interestingly, we also identified novel membrane-bound transcription factors that may be subject to ubiquitin/proteasome-mediated cleavage and activation at the ER membrane.
  66. Kaminska J, Spiess M, Stawiecka Mirota M, Monkaityte R, Haguenauer Tsapis R, Urban Grimal D, et al. Yeast Rsp5 ubiquitin ligase affects the actin cytoskeleton in vivo and in vitro. Eur J Cell Biol. 2011;90:1016-28 pubmed publisher
    Yeast Rsp5 ubiquitin ligase is involved in several cellular processes, including endocytosis. Actin patches are sites of endocytosis, a process involving actin assembly and disassembly...
  67. Ziv I, Matiuhin Y, Kirkpatrick D, Erpapazoglou Z, Leon S, Pantazopoulou M, et al. A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis. Mol Cell Proteomics. 2011;10:M111.009753 pubmed publisher
    Any of seven lysine residues on ubiquitin can serve as the base for chain-extension, resulting in a sizeable spectrum of ubiquitin modifications differing in chain length or linkage type...
  68. Vitaliano Prunier A, Menant A, Hobeika M, Geli V, Gwizdek C, Dargemont C. Ubiquitylation of the COMPASS component Swd2 links H2B ubiquitylation to H3K4 trimethylation. Nat Cell Biol. 2008;10:1365-71 pubmed publisher
    ..Our results further indicate that Swd2 is a major H3-binding component of COMPASS. Swd2 thus represents a key factor that mediates crosstalk between H2B ubiquitylation and H3K4 trimethylation on chromatin. ..
  69. Archer C, Delahodde A, Gonzalez F, Johnston S, Kodadek T. Activation domain-dependent monoubiquitylation of Gal4 protein is essential for promoter binding in vivo. J Biol Chem. 2008;283:12614-23 pubmed publisher
    ..presented that the piece of the AD that is deleted in Gal4D protein is likely a recognition element for the E3 ubiquitin-protein ligase that modifies Gal4...
  70. Swanson K, Hicke L, Radhakrishnan I. Structural basis for monoubiquitin recognition by the Ede1 UBA domain. J Mol Biol. 2006;358:713-24 pubmed
    ..Ede1 protein functions at the internalization step of endocytosis and binds monoubiquitinated proteins through a ubiquitin associated (UBA) domain...
  71. Eguez L, Chung Y, Kuchibhatla A, Paidhungat M, Garrett S. Yeast Mn2+ transporter, Smf1p, is regulated by ubiquitin-dependent vacuolar protein sorting. Genetics. 2004;167:107-17 pubmed
    ..Because Smf1p is ubiquitinated in vivo, we propose that Smf1p is targeted to the vacuole for degradation by ubiquitination-dependent protein sorting. ..
  72. Ren J, Pashkova N, Winistorfer S, Piper R. DOA1/UFD3 plays a role in sorting ubiquitinated membrane proteins into multivesicular bodies. J Biol Chem. 2008;283:21599-611 pubmed publisher
    b>Ubiquitin (Ub) is a sorting signal that targets integral membrane proteins to the interior of the vacuole/lysosome by directing them into lumenal vesicles of multivesicular bodies (MVBs)...
  73. Saugar I, Parker J, Zhao S, Ulrich H. The genome maintenance factor Mgs1 is targeted to sites of replication stress by ubiquitylated PCNA. Nucleic Acids Res. 2012;40:245-57 pubmed publisher
    ..A ubiquitin-binding zinc finger (UBZ) domain directs human WRNIP1 to nuclear foci, but the functional significance of its ..
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    In addition to its well known role in targeting proteins for proteasomal degradation, ubiquitin (Ub) is also involved in promoting internalization of cell surface proteins into the endocytic pathway...
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    ..of a MVB is a highly regulated process that is positively modulated by covalent modification of cargoes with ubiquitin. To identify additional MVB sorting signals, we examined the previously described ubiquitination-independent MVB ..
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    Ubc7p is a ubiquitin-conjugating enzyme (E2) that functions with endoplasmic reticulum (ER)-resident ubiquitin ligases (E3s) to promote endoplasmic reticulum-associated degradation (ERAD)...
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    A growing number of yeast and mammalian plasma membrane proteins are reported to be modified with K63-linked ubiquitin (Ub) chains...