Gene Symbol: UAF30
Species: Saccharomyces cerevisiae S288c
Siddiqi I, Dodd J, Vu L, Eliason K, Oakes M, Keener J, et al
. Transcription of chromosomal rRNA genes by both RNA polymerase I and II in yeast uaf30 mutants lacking the 30 kDa subunit of transcription factor UAF. EMBO J. 2001;20:4512-21 pubmed
..Thus, Uaf30p plays only a minor role in its activator function...
Hontz R, French S, Oakes M, Tongaonkar P, Nomura M, Beyer A, et al
. Transcription of multiple yeast ribosomal DNA genes requires targeting of UAF to the promoter by Uaf30. Mol Cell Biol. 2008;28:6709-19 pubmed publisher
..The results show that Uaf30p is a key targeting factor for the UAF complex that facilitates activation of a large proportion of rDNA genes in ..
Oakes M, Siddiqi I, French S, Vu L, Sato M, Aris J, et al
. Role of histone deacetylase Rpd3 in regulating rRNA gene transcription and nucleolar structure in yeast. Mol Cell Biol. 2006;26:3889-901 pubmed
Merz K, Hondele M, Goetze H, Gmelch K, Stoeckl U, Griesenbeck J. Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules. Genes Dev. 2008;22:1190-204 pubmed publisher
..We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1. ..
Goetze H, Wittner M, Hamperl S, Hondele M, Merz K, Stoeckl U, et al
. Alternative chromatin structures of the 35S rRNA genes in Saccharomyces cerevisiae provide a molecular basis for the selective recruitment of RNA polymerases I and II. Mol Cell Biol. 2010;30:2028-45 pubmed publisher
..Furthermore, lack of UAF leads to the loss of Sir2 from rDNA, correlating with impaired Pol II silencing. This analysis of rDNA chromatin provides a molecular basis, explaining many phenotypes observed in previous genetic analyses. ..
Bernstein K, Reid R, Sunjevaric I, Demuth K, Burgess R, Rothstein R. The Shu complex, which contains Rad51 paralogues, promotes DNA repair through inhibition of the Srs2 anti-recombinase. Mol Biol Cell. 2011;22:1599-607 pubmed publisher
..ribosomal DNA (rDNA) recombination when we found an interaction between one member of the Shu complex, SHU1, and UAF30, a component of the upstream activating factor complex (UAF), which regulates rDNA transcription...
Ha C, Sung M, Huh W. Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:4892-903 pubmed publisher
..Furthermore, Nsi1 contributes to the longevity of yeast cells. Taken together, our findings suggest that Nsi1 is a new rDNA silencing factor that contributes to rDNA stability and lifespan extension in S. cerevisiae. ..
Bernstein K, Juanchich A, Sunjevaric I, Rothstein R. The Shu complex regulates Rad52 localization during rDNA repair. DNA Repair (Amst). 2013;12:786-90 pubmed publisher
..plays an important role in ribosomal DNA (rDNA) recombination when the Upstream Activating Factor (UAF) protein Uaf30 is disrupted...
Zhang Y, French S, Beyer A, Schneider D. The Transcription Factor THO Promotes Transcription Initiation and Elongation by RNA Polymerase I. J Biol Chem. 2016;291:3010-8 pubmed publisher
..Together, these data show that perturbation of THO function impairs transcription initiation and elongation by Pol I, identifying a new cellular target for the conserved THO complex. ..