Gene Symbol: TUM1
Description: thiosulfate sulfurtransferase
Alias: thiosulfate sulfurtransferase
Species: Saccharomyces cerevisiae S288c
Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
Poirey R, Jauniaux J. Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1. Yeast. 1997;13:483-7 pubmed
..The sequence encodes 26 open reading frames of at least 100 amino acids. Eight of these correspond to known genes, whereas 18 correspond to new genes. ..
Noma A, Sakaguchi Y, Suzuki T. Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res. 2009;37:1335-52 pubmed publisher
..We have identified five genes in Saccharomyces cerevisiae, YIL008w (URM1), YHR111w (UBA4), YOR251c (TUM1), YNL119w (NCS2) and YGL211w (NCS6), that are required for 2-thiolation of mcm(5)s(2)U...
Qiu R, Wang F, Liu M, Lou T, Ji C. Crystal structure of the Tum1 protein from the yeast Saccharomyces cerevisiae. Protein Pept Lett. 2012;19:1139-43 pubmed
Yeast tRNA-thiouridine modification protein 1 (Tum1) plays essential role in the sulfur transfer process of Urm1 system, which in turn is involved in many important cellular processes...
Huang C, Walker M, Fedrizzi B, Roncoroni M, Gardner R, Jiranek V. The yeast TUM1 affects production of hydrogen sulfide from cysteine treatment during fermentation. FEMS Yeast Res. 2016;16: pubmed publisher
..For the first time, we show that TUM1 is partly responsible for the early production of H2S from cysteine...
Huang C, Walker M, Fedrizzi B, Gardner R, Jiranek V. Yeast genes involved in regulating cysteine uptake affect production of hydrogen sulfide from cysteine during fermentation. FEMS Yeast Res. 2017;17: pubmed publisher
..The findings highlight that cysteine transportation could be a limiting factor for yeast to generate H2S from cysteine, and therefore selecting wine yeasts without defects in cysteine uptake could maximise thiol production potential. ..
Qiu R, Wang F, Liu M, Yang Z, Wu T, Ji C. Crystallization and preliminary X-ray analysis of the yeast tRNA-thiouridine modification protein 1 (Tum1p). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:953-5 pubmed publisher
..94, c = 48.35?Å. The asymmetric unit of the crystal was assumed to contain one protein molecule, giving a Matthews coefficient of 2.41?Å(3)?Da(-1) and a solvent content of 49.0%. ..