TUB1

Summary

Gene Symbol: TUB1
Description: alpha-tubulin TUB1
Alias: alpha-tubulin TUB1
Species: Saccharomyces cerevisiae S288c
Products:     TUB1

Top Publications

  1. Sproul L, Anderson D, Mackey A, Saunders W, Gilbert S. Cik1 targets the minus-end kinesin depolymerase kar3 to microtubule plus ends. Curr Biol. 2005;15:1420-7 pubmed
    ..We propose that Cik1 targets Kar3 to the microtubule plus end. Kar3Cik1 then uses its minus-end-directed force to depolymerize microtubules from the plus end, with each tubulin-subunit release event tightly coupled to one ATP turnover. ..
  2. Ayaz P, Ye X, Huddleston P, Brautigam C, Rice L. A TOG:??-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase. Science. 2012;337:857-60 pubmed publisher
    ..Conformation-selective interactions with ??-tubulin explain how TOG-containing polymerases discriminate between unpolymerized and polymerized forms of ??-tubulin and how they selectively recognize the growing end of the microtubule. ..
  3. Johnson V, Ayaz P, Huddleston P, Rice L. Design, overexpression, and purification of polymerization-blocked yeast ??-tubulin mutants. Biochemistry. 2011;50:8636-44 pubmed publisher
  4. Chen X, Yin H, Huffaker T. The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly. J Cell Biol. 1998;141:1169-79 pubmed
    ..The chromosome segregation block does not arrest the cell cycle; instead, spc72 cells undergo cytokinesis, producing aploid cells and polyploid cells that contain multiple SPBs. ..
  5. Lacefield S, Magendantz M, Solomon F. Consequences of defective tubulin folding on heterodimer levels, mitosis and spindle morphology in Saccharomyces cerevisiae. Genetics. 2006;173:635-46 pubmed
  6. Archer J, Magendantz M, Vega L, Solomon F. Formation and function of the Rbl2p-beta-tubulin complex. Mol Cell Biol. 1998;18:1757-62 pubmed
    ..They also suggest that the Rbl2p-beta-tubulin complex is part of a cellular mechanism for regulating the levels and dimerization of tubulin chains. ..
  7. Berlin V, Styles C, Fink G. BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin. J Cell Biol. 1990;111:2573-86 pubmed
    ..Based on these results, we propose that BIK1 is required stoichiometrically for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation. ..
  8. Hoyt M, Macke J, Roberts B, Geiser J. Saccharomyces cerevisiae PAC2 functions with CIN1, 2 and 4 in a pathway leading to normal microtubule stability. Genetics. 1997;146:849-57 pubmed
    ..Our findings suggest that the guanine nucleotide-binding Cin4p interacts with Cin1p and regulates its tubulin folding activity. ..
  9. Al Bassam J, van Breugel M, Harrison S, Hyman A. Stu2p binds tubulin and undergoes an open-to-closed conformational change. J Cell Biol. 2006;172:1009-22 pubmed
    ..We suggest that this mechanism of microtubule regulation is a conserved feature of the Dis1/XMAP215 family of MAPs. ..

More Information

Publications62

  1. Schwartz K, Richards K, Botstein D. BIM1 encodes a microtubule-binding protein in yeast. Mol Biol Cell. 1997;8:2677-91 pubmed
    ..was obtained from a two-hybrid screen of a yeast cDNA library using as bait the entire coding sequence of TUB1 (encoding alpha-tubulin)...
  2. Vega L, Fleming J, Solomon F. An alpha-tubulin mutant destabilizes the heterodimer: phenotypic consequences and interactions with tubulin-binding proteins. Mol Biol Cell. 1998;9:2349-60 pubmed
    ..Here we use a mutant alpha-tubulin to probe cellular regulation of microtubule assembly. tub1-724 mutant cells arrest at low temperature with no assembled microtubules...
  3. Stearns T, Botstein D. Unlinked noncomplementation: isolation of new conditional-lethal mutations in each of the tubulin genes of Saccharomyces cerevisiae. Genetics. 1988;119:249-60 pubmed
    ..The unlinked mutation was shown to be a conditional-lethal allele of the major alpha-tubulin-encoding gene (TUB1) and represents the first such mutation in that gene...
  4. Caudron F, Andrieux A, Job D, Boscheron C. A new role for kinesin-directed transport of Bik1p (CLIP-170) in Saccharomyces cerevisiae. J Cell Sci. 2008;121:1506-13 pubmed publisher
    ..These results indicate that Bik1p interactions with tubulin are important for Bik1p plus-end tracking, and suggest alternative pathways for Bik1p-Kip2p-dependent dynein localization at plus ends. ..
  5. Burns C, Ohi R, Mehta S, O TOOLE E, Winey M, Clark T, et al. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:801-15 pubmed
    ..pre-mRNA splicing discovered through intron microarray analysis, inefficient splicing of the alpha-tubulin-encoding TUB1 mRNA was considered as a potential cause of the cef1-13 cell cycle arrest because cef1-13 cells arrest uniformly at ..
  6. Su X, Qiu W, Gupta M, Pereira Leal J, Reck Peterson S, Pellman D. Mechanisms underlying the dual-mode regulation of microtubule dynamics by Kip3/kinesin-8. Mol Cell. 2011;43:751-63 pubmed publisher
    ..We propose a concentration-dependent model for the coordination of the destabilizing and stabilizing activities of Kip3 and discuss its relevance to cellular microtubule organization. ..
  7. Abruzzi K, Magendantz M, Solomon F. An alpha-tubulin mutant demonstrates distinguishable functions among the spindle assembly checkpoint genes in Saccharomyces cerevisiae. Genetics. 2002;161:983-94 pubmed
    Cells expressing a mutant allele of alpha-tubulin, tub1-729, are cold sensitive and arrest as large-budded cells with microtubule defects...
  8. Laflamme G, Tremblay Boudreault T, Roy M, Andersen P, Bonneil E, Atchia K, et al. Structural maintenance of chromosome (SMC) proteins link microtubule stability to genome integrity. J Biol Chem. 2014;289:27418-31 pubmed publisher
    ..Collectively, these findings demonstrate that SMC proteins can bind to and stabilize microtubules and that SMC-microtubule interactions are essential to establish a robust system to maintain genome integrity. ..
  9. Fleming J, Vega L, Solomon F. Function of tubulin binding proteins in vivo. Genetics. 2000;156:69-80 pubmed
    ..of the beta-tubulin binding protein Rbl2p/cofactor A is lethal in yeast cells expressing a mutant alpha-tubulin, tub1-724, that produces unstable heterodimer...
  10. Kirkpatrick D, Solomon F. Overexpression of yeast homologs of the mammalian checkpoint gene RCC1 suppresses the class of alpha-tubulin mutations that arrest with excess microtubules. Genetics. 1994;137:381-92 pubmed
    ..cerevisiae gene SRM1/PRP20. Overexpression of SRM1/PRP20 also suppresses class 2 mutants. The results suggest that this family of genes may participate in regulatory interactions between microtubules and the cell cycle. ..
  11. Geissler S, Siegers K, Schiebel E. A novel protein complex promoting formation of functional alpha- and gamma-tubulin. EMBO J. 1998;17:952-66 pubmed
    ..We suggest that the Gim proteins form a protein complex that promotes formation of functional alpha- and gamma-tubulin...
  12. Feierbach B, Nogales E, Downing K, Stearns T. Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin. J Cell Biol. 1999;144:113-24 pubmed
    ..The phenotype of overexpression of ALF1 suggests that Alf1p can act to sequester alpha-tubulin from interaction with beta-tubulin, raising the possibility that it plays a regulatory role in the formation of the tubulin heterodimer. ..
  13. Ursic D, Sedbrook J, Himmel K, Culbertson M. The essential yeast Tcp1 protein affects actin and microtubules. Mol Biol Cell. 1994;5:1065-80 pubmed
    ..indicative of allele-specific genetic interactions were observed when tcp1-1 was combined in the same strain with tub1-1, tub2-402, act1-1, and act1-4, but not with other tubulin or actin mutations or with mutations in other genes ..
  14. Bock L, Pagliuca C, Kobayashi N, Grove R, Oku Y, Shrestha K, et al. Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nat Cell Biol. 2012;14:614-24 pubmed publisher
    ..Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases. ..
  15. Laan L, Pavin N, Husson J, Romet Lemonne G, van Duijn M, López M, et al. Cortical dynein controls microtubule dynamics to generate pulling forces that position microtubule asters. Cell. 2012;148:502-14 pubmed publisher
    ..Our results demonstrate the intrinsic ability of cortical microtubule-dynein interactions to regulate microtubule dynamics and drive positioning processes in living cells. ..
  16. Pablo Hernando M, Arnaiz Pita Y, Tachikawa H, del Rey F, Neiman A, Vazquez de Aldana C. Septins localize to microtubules during nutritional limitation in Saccharomyces cerevisiae. BMC Cell Biol. 2008;9:55 pubmed publisher
    ..Septin association to microtubules also occurs during growth in non-fermentable carbon sources. ..
  17. Cohnen A, Bielig H, Hollenberg C, Hu Z, Ramezani Rad M. The yeast ubiquitin-like domain protein Mdy2 is required for microtubule-directed nuclear migration and localizes to cytoplasmic granules in response to heat stress. Cytoskeleton (Hoboken). 2010;67:635-49 pubmed publisher
    ..Accordingly, we propose that Mdy2 plays a critical role in the MT-dependent processes of karyogamy and stress response. ..
  18. Lang T, Schaeffeler E, Bernreuther D, Bredschneider M, Wolf D, Thumm M. Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole. EMBO J. 1998;17:3597-607 pubmed
    ..Our findings suggest that microtubules and an attached protein complex of Aut2p and Aut7p are involved in the delivery of autophagic vesicles to the vacuole. ..
  19. Arellano Santoyo H, Geyer E, Stokasimov E, Chen G, Su X, Hancock W, et al. A Tubulin Binding Switch Underlies Kip3/Kinesin-8 Depolymerase Activity. Dev Cell. 2017;42:37-51.e8 pubmed publisher
    ..Together, these findings elucidate a major regulatory mechanism controlling the size of cellular microtubule structures. ..
  20. Boscheron C, Caudron F, Loeillet S, Peloso C, Mugnier M, Kurzawa L, et al. A role for the yeast CLIP170 ortholog, the plus-end-tracking protein Bik1, and the Rho1 GTPase in Snc1 trafficking. J Cell Sci. 2016;129:3332-41 pubmed publisher
    ..To address the physiological role of the C-terminal aromatic residue of ?-tubulin, a tub1-Glu yeast strain expressing an ?-tubulin devoid of its C-terminal amino acid was used to perform a genome-wide-..
  21. Haldar S, Sarkar S, Singh V, Sinha P. High copies of SUM1 enhance the stability of wild-type microtubules against adverse conditions in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2012;418:525-30 pubmed publisher
    ..plasmid (2 ?-SUM1), suppressed cold-sensitive growth phenotype associated with mutations in the ?-tubulin gene TUB1. In this work we show that the dosage suppression is accompanied by an elevation of ?-tubulin levels in mutant ..
  22. Voloshin O, Gocheva Y, Gutnick M, Movshovich N, Bakhrat A, Baranes Bachar K, et al. Tubulin chaperone E binds microtubules and proteasomes and protects against misfolded protein stress. Cell Mol Life Sci. 2010;67:2025-38 pubmed publisher
    ..We propose a novel role for Pac2 in the misfolded protein stress response based on its ability to interact with both the MT cytoskeleton and the proteasomes. ..
  23. Markus S, Kalutkiewicz K, Lee W. She1-mediated inhibition of dynein motility along astral microtubules promotes polarized spindle movements. Curr Biol. 2012;22:2221-30 pubmed publisher
    ..Our data reveal how inhibitory microtubule-associated proteins selectively regulate motor activity to achieve unidirectional nuclear transport and demonstrate a direct link between cell-cycle machinery and dynein pathway activity. ..
  24. Gonzalez M, Cope J, Rank K, Chen C, Tittmann P, Rayment I, et al. Common mechanistic themes for the powerstroke of kinesin-14 motors. J Struct Biol. 2013;184:335-44 pubmed publisher
  25. Li X, Rayman J, Kandel E, Derkatch I. Functional role of Tia1/Pub1 and Sup35 prion domains: directing protein synthesis machinery to the tubulin cytoskeleton. Mol Cell. 2014;55:305-18 pubmed publisher
    ..This Sup35/Pub1 complex, which also contains TUB1 mRNA and components of translation machinery, is important for the integrity of the tubulin cytoskeleton: PUB1 ..
  26. Woodruff J, Drubin D, Barnes G. Spindle assembly requires complete disassembly of spindle remnants from the previous cell cycle. Mol Biol Cell. 2012;23:258-67 pubmed publisher
    ..Therefore we propose that spindle disassembly is essential for regeneration of the intracellular pool of assembly-competent tubulin required for efficient spindle assembly during subsequent mitoses of daughter cells. ..
  27. Alvarez P, Smith A, Fleming J, Solomon F. Modulation of tubulin polypeptide ratios by the yeast protein Pac10p. Genetics. 1998;149:857-64 pubmed
    ..Pac10 null cells show a 30% decrease in the ratio of alpha-tubulin to beta-tubulin. The results suggest that Pac10p modulates the level of alpha-tubulin in the cell, and so influences microtubule morphogenesis and tubulin metabolism. ..
  28. Richards K, Anders K, Nogales E, Schwartz K, Downing K, Botstein D. Structure-function relationships in yeast tubulins. Mol Biol Cell. 2000;11:1887-903 pubmed
    A comprehensive set of clustered charged-to-alanine mutations was generated that systematically alter TUB1, the major alpha-tubulin gene of Saccharomyces cerevisiae...
  29. Kammerer D, Stevermann L, Liakopoulos D. Ubiquitylation regulates interactions of astral microtubules with the cleavage apparatus. Curr Biol. 2010;20:1233-43 pubmed publisher
    ..Photoconversion experiments showed that Kar9 association with aMTs is stable. We propose that ubiquitylation controls interactions of aMTs with the cleavage apparatus through localized disassembly of Kar9 complexes. ..
  30. Schatz P, Solomon F, Botstein D. Isolation and characterization of conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae. Genetics. 1988;120:681-95 pubmed
    ..We have isolated 70 conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae using a plasmid replacement technique...
  31. Markus S, Punch J, Lee W. Motor- and tail-dependent targeting of dynein to microtubule plus ends and the cell cortex. Curr Biol. 2009;19:196-205 pubmed publisher
    ..Our results suggest that the cortical association domain is normally masked in the full-length dynein molecule. We propose that targeting of dynein to plus ends unmasks the tail, priming the motor for off-loading to cortical Num1 sites. ..
  32. Irminger Finger I, Mathis N. Effect of microtubule-associated protein MHP1 on microtubule assembly and cell cycle progression in Saccharomyces cerevisiae. Cell Struct Funct. 1998;23:209-19 pubmed
    ..These data indicate that Mhplp not only stabilizes microtubules but promotes microtubule assembly in vivo, and suggest that the role of other mammalian MAPs in the promotion of microtubule assembly could be tested in this yeast system. ..
  33. Sweet T, Boyer B, Hu W, Baker K, Coller J. Microtubule disruption stimulates P-body formation. RNA. 2007;13:493-502 pubmed
    ..we also demonstrate that disruption of microtubules by a temperature-sensitive allele of the major alpha tubulin, TUB1 (tub1-724) stimulates P-body formation...
  34. Makanae K, Kintaka R, Makino T, Kitano H, Moriya H. Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method. Genome Res. 2013;23:300-11 pubmed publisher
    ..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition. ..
  35. Gao L, Bretscher A. Analysis of unregulated formin activity reveals how yeast can balance F-actin assembly between different microfilament-based organizations. Mol Biol Cell. 2008;19:1474-84 pubmed publisher
    ..Thus, cells must have a way of ensuring a proper balance between actin assembly pathways. ..
  36. Leduc C, Padberg Gehle K, Varga V, Helbing D, Diez S, Howard J. Molecular crowding creates traffic jams of kinesin motors on microtubules. Proc Natl Acad Sci U S A. 2012;109:6100-5 pubmed publisher
    ..Our results indicate that transport kinesins, such as kinesin-1, may be evolutionarily adapted to avoid the formation of traffic jams by moving only with moderate processivity and dissociating rapidly from microtubule ends. ..
  37. Interthal H, Bellocq C, Bahler J, Bashkirov V, Edelstein S, Heyer W. A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae. EMBO J. 1995;14:1057-66 pubmed
    ..Taken together these three lines of evidence constitute compelling evidence for a role of Sep1 as an accessory protein in microtubule function in the yeast S.cerevisiae. ..
  38. Dong C, Lin Z, Diao W, Li D, Chu X, Wang Z, et al. The Elp2 subunit is essential for elongator complex assembly and functional regulation. Structure. 2015;23:1078-86 pubmed publisher
    ..Our results indicate that Elp2 is a necessary component for functional Elongator and acts as a hub in the formation of various complexes. ..
  39. Ghosh S, Poddar A, Hajra S, Sanyal K, Sinha P. The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation. Mol Genet Genomics. 2001;265:249-57 pubmed
    ..However, the mcm19 null mutation conferred growth defects in the presence of a mutation in the TUB1 gene coding for alpha-tubulin...
  40. Sarkar S, Haldar S, Hajra S, Sinha P. The budding yeast protein Sum1 functions independently of its binding partners Hst1 and Sir2 histone deacetylases to regulate microtubule assembly. FEMS Yeast Res. 2010;10:660-73 pubmed publisher
    ..has been identified as an allele-specific dosage suppressor of mutations in the major alpha-tubulin-coding gene TUB1. When cloned in a 2mu vector, SUM1 suppressed the cold-sensitive and benomyl-hypersensitive phenotypes associated ..
  41. Hasek J, Kovarik P, VALASEK L, Malinska K, Schneider J, Kohlwein S, et al. Rpg1p, the subunit of the Saccharomyces cerevisiae eIF3 core complex, is a microtubule-interacting protein. Cell Motil Cytoskeleton. 2000;45:235-46 pubmed
    ..We conclude that Rpg1p is a microtubule-interacting protein that indicates an interesting connection between the translation initiation machinery and cytoskeleton in yeast Saccharomyces cerevisiae. ..
  42. Ayaz P, Munyoki S, Geyer E, Piedra F, Vu E, Bromberg R, et al. A tethered delivery mechanism explains the catalytic action of a microtubule polymerase. elife. 2014;3:e03069 pubmed publisher
    ..This tethering model can explain catalyst-like behavior and also predicts that the polymerase action changes the configuration of the microtubule end...
  43. Erlemann S, Neuner A, Gombos L, Gibeaux R, Antony C, Schiebel E. An extended ?-tubulin ring functions as a stable platform in microtubule nucleation. J Cell Biol. 2012;197:59-74 pubmed publisher
    ..Nucleation and anchoring of MTs required the same number of ?-tubulin molecules. We suggest that a spiral of seven ?-TuSCs with a slight surplus of ?-tubulin nucleates MTs in vivo. ..
  44. Chawla G, Sapra A, Surana U, Vijayraghavan U. Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions. Nucleic Acids Res. 2003;31:2333-43 pubmed
    ..Splicing of TUB1 and TUB3 transcripts, which encode alpha-tubulin, was analyzed in prp17 and other second-step factor mutants...
  45. Farkasovsky M, Küntzel H. Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions. J Cell Biol. 1995;131:1003-14 pubmed
    ..A num1 null mutation confers temperature sensitivity to the cold-sensitive alpha-tubulin mutant tub1-1, and shows synthetic lethality with the beta-tubulin mutant alleles tub2-402, tub2-403, tub2-404, and tub2-405...
  46. Campetelli A, Monesterolo N, Previtali G, Santander V, Amaiden M, Arce C, et al. Activation of H(+)-ATPase by glucose in Saccharomyces cerevisiae involves a membrane serine protease. Biochim Biophys Acta. 2013;1830:3593-603 pubmed publisher
    ..This finding also proposes an indirect way to pharmacologically regulate yeast H(+)-ATPase activity and open the question about mechanistic similarities with other higher eukaryotes. ..
  47. Howes S, Geyer E, LaFrance B, Zhang R, Kellogg E, Westermann S, et al. Structural differences between yeast and mammalian microtubules revealed by cryo-EM. J Cell Biol. 2017;216:2669-2677 pubmed publisher
    ..These differences may reflect adaptations to the demands of different cell size or range of physiological growth temperatures. ..
  48. Lahiri S, Mehta G, Ghosh S. Iml3p, a component of the Ctf19 complex of the budding yeast kinetochore is required to maintain kinetochore integrity under conditions of spindle stress. FEMS Yeast Res. 2013;13:375-85 pubmed publisher
    ..assembly of kinetochore proteins except one that lacked CHL4 and IML3 genes in a tubulin-defective background (tub1-1 chl4 iml3)...
  49. Funk C, Schmeiser V, Ortiz J, Lechner J. A TOGL domain specifically targets yeast CLASP to kinetochores to stabilize kinetochore microtubules. J Cell Biol. 2014;205:555-71 pubmed publisher
    ..In both phases, the activity of TOGL2 is essential for interpolar MT stability, whereas TOGL1 is not involved. Thus, the two TOGL domains of yeast CLASP have different activities and execute distinct mitotic functions. ..
  50. Schwock J, Kirchberger J, Edelmann A, Kriegel T, Kopperschläger G. Interaction of 6-phosphofructokinase with cytosolic proteins of Saccharomyces cerevisiae. Yeast. 2004;21:483-94 pubmed
    ..cerevisiae mutant strains defective in various heat shock proteins (TRiC/CCT, Hsp70, Hsp 104) and in the respective wild-type background did not reveal significant differences. ..
  51. You L, Gillilan R, Huffaker T. Model for the yeast cofactor A-beta-tubulin complex based on computational docking and mutagensis. J Mol Biol. 2004;341:1343-54 pubmed
    ..Rbl2 and alpha-tubulin share overlapping binding sites on the beta-tubulin molecule providing a structural explanation for the mutually exclusive binding of Rbl2 and alpha-tubulin to beta-tubulin. ..
  52. Rank K, Chen C, Cope J, Porche K, Hoenger A, Gilbert S, et al. Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern. J Cell Biol. 2012;197:957-70 pubmed publisher
    ..The results indicate that head-head communication is mediated through the adjoining coiled coil. ..
  53. Guenette S, Magendantz M, Solomon F. Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes. J Cell Sci. 1995;108 ( Pt 3):1195-204 pubmed
    ..Cells expressing the recessive allele tub1-729 as their sole alpha-tubulin gene grow normally at permissive temperature...