TSC11

Summary

Gene Symbol: TSC11
Description: TORC2 complex subunit TSC11
Alias: AVO3, TORC2 complex subunit TSC11
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Beeler T, Bacikova D, Gable K, Hopkins L, Johnson C, Slife H, et al. The Saccharomyces cerevisiae TSC10/YBR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2Delta mutant. J Biol Chem. 1998;273:30688-94 pubmed
    ..The recombinant protein catalyzes the NADPH-dependent reduction of 3-ketosphinganine. These data indicate that Tsc10p is necessary and sufficient for catalyzing the NADPH-dependent reduction of 3-ketosphinganine to dihydrosphingosine. ..
  2. Mnaimneh S, Davierwala A, Haynes J, Moffat J, Peng W, Zhang W, et al. Exploration of essential gene functions via titratable promoter alleles. Cell. 2004;118:31-44 pubmed
    ..We furthermore show that these strains are compatible with automated genetic analysis. This study underscores the importance of analyzing mutant phenotypes and provides a resource to complement the yeast knockout collection. ..
  3. Ho H, Shiau Y, Chen M. Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex. Curr Genet. 2005;47:273-88 pubmed
    Saccharomyces cerevisiae TSC11/AVO3 is an essential gene encoding one component of TORC2, a multi-protein complex of yeast Tor2p that also contains Lst8p, Avo1p, and Avo2p...
  4. Wullschleger S, Loewith R, Oppliger W, Hall M. Molecular organization of target of rapamycin complex 2. J Biol Chem. 2005;280:30697-704 pubmed
    ..cytoskeleton through a rapamycin-insensitive signaling branch and in yeast consists of the six proteins AVO1, AVO2, AVO3, BIT61, LST8, and TOR2. Here we have focused on the characterization of TORC2...
  5. Mulet J, Martin D, Loewith R, Hall M. Mutual antagonism of target of rapamycin and calcineurin signaling. J Biol Chem. 2006;281:33000-7 pubmed
    ..Thus, TORC2-SLM signaling negatively regulates calcineurin, and calcineurin negatively regulates TORC2-SLM. These findings provide a molecular basis for the mutual antagonism of growth and stress. ..
  6. Aronova S, Wedaman K, Aronov P, Fontes K, Ramos K, Hammock B, et al. Regulation of ceramide biosynthesis by TOR complex 2. Cell Metab. 2008;7:148-58 pubmed publisher
    ..Because Ypk2 is activated independently by LCBs, the direct precursors to ceramides, our data suggest a model wherein TORC2 signaling is coupled with LCB levels to control Ypk2 activity and, ultimately, regulate ceramide formation. ..
  7. Niles B, Joslin A, Fresques T, Powers T. TOR complex 2-Ypk1 signaling maintains sphingolipid homeostasis by sensing and regulating ROS accumulation. Cell Rep. 2014;6:541-52 pubmed publisher
    ..Thus, ROS sensing and signaling by TORC2/Ypk1 play a central physiological role in sphingolipid biosynthesis and in the maintenance of cell growth and viability. ..
  8. Niles B, Mogri H, Hill A, Vlahakis A, Powers T. Plasma membrane recruitment and activation of the AGC kinase Ypk1 is mediated by target of rapamycin complex 2 (TORC2) and its effector proteins Slm1 and Slm2. Proc Natl Acad Sci U S A. 2012;109:1536-41 pubmed publisher
    ..These findings both extend the scope of cellular processes regulated by Ypk1/2 to include negative regulation of calcineurin and broaden the repertoire of mechanisms for membrane recruitment and activation of a protein kinase. ..
  9. Gaubitz C, Oliveira T, Prouteau M, Leitner A, Karuppasamy M, Konstantinidou G, et al. Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2. Mol Cell. 2015;58:977-88 pubmed publisher
    ..Our data indicate that the C-terminal part of Avo3, a subunit unique to TORC2, is close to the FKBP12-rapamycin-binding domain of Tor2...

More Information

Publications18

  1. Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo J, Bonenfant D, et al. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002;10:457-68 pubmed
    ..TORC2 contains TOR2, AVO1 (YOL078w), AVO2 (YMR068w), AVO3 (YER093c), and LST8...
  2. Liao H, Chen M. Target of rapamycin complex 2 signals to downstream effector yeast protein kinase 2 (Ypk2) through adheres-voraciously-to-target-of-rapamycin-2 protein 1 (Avo1) in Saccharomyces cerevisiae. J Biol Chem. 2012;287:6089-99 pubmed publisher
    ..Employing avo3(ts) mutants with defects in TORC2 functions that are suppressible by active Ypk2, we investigated the molecular ..
  3. Muir A, Roelants F, Timmons G, Leskoske K, Thorner J. Down-regulation of TORC2-Ypk1 signaling promotes MAPK-independent survival under hyperosmotic stress. elife. 2015;4: pubmed publisher
    ..These events are all Hog1-independent. Our findings define the underlying molecular basis of a new mechanism for responding to hypertonic conditions. ..
  4. Nomura W, Inoue Y. Methylglyoxal activates the target of rapamycin complex 2-protein kinase C signaling pathway in Saccharomyces cerevisiae. Mol Cell Biol. 2015;35:1269-80 pubmed publisher
    ..Methylglyoxal activated mammalian TORC2 signaling, which, in turn, phosphorylated Akt at Ser(473). Our results suggest that methylglyoxal is a conserved initiator of TORC2 signaling among eukaryotes. ..
  5. Kennedy M, Gable K, Niewola Staszkowska K, Abreu S, Johnston A, Harris L, et al. A neurotoxic glycerophosphocholine impacts PtdIns-4, 5-bisphosphate and TORC2 signaling by altering ceramide biosynthesis in yeast. PLoS Genet. 2014;10:e1004010 pubmed publisher
  6. Ho H, Lee H, Liao H, Chen M. Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signaling. Eukaryot Cell. 2008;7:1328-43 pubmed publisher
    ..Two classes of avo3 temperature-sensitive (avo3(ts)) mutants that we previously identified display cell integrity and actin defects, ..
  7. Niles B, Powers T. TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species. Mol Biol Cell. 2014;25:3962-72 pubmed publisher
  8. Reinke A, Anderson S, McCaffery J, Yates J, Aronova S, Chu S, et al. TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem. 2004;279:14752-62 pubmed
    ..Together, these results define a novel connection between TORC1 and Ssd1p-mediated maintenance of cellular integrity. ..
  9. Wedaman K, Reinke A, Anderson S, Yates J, McCaffery J, Powers T. Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:1204-20 pubmed
    ..That these complexes colocalize to a membrane-bound compartment suggests an intimate relationship between membrane-mediated signaling and Tor activity. ..