Gene Symbol: TRM9
Description: tRNA (carboxymethyluridine(34)-5-O)-methyltransferase
Alias: KTI1, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kalhor H, Clarke S. Novel methyltransferase for modified uridine residues at the wobble position of tRNA. Mol Cell Biol. 2003;23:9283-92 pubmed
    We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation ..
  2. Johansson M, Esberg A, Huang B, Bjork G, Byström A. Eukaryotic wobble uridine modifications promote a functionally redundant decoding system. Mol Cell Biol. 2008;28:3301-12 pubmed publisher
    ..Moreover, the observation that the mcm(5)U(34)- and some ncm(5)U(34)-containing tRNAs efficiently read G-ending codons challenges the notion that eukaryotes do not use U-G wobbling. ..
  3. Studte P, Zink S, Jablonowski D, Bär C, von der Haar T, Tuite M, et al. tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol. 2008;69:1266-77 pubmed publisher
    ..Hence, U34 modification defects including lack of the U34 tRNA methyltransferase Trm9 protect against tRNA cleavage and zymocin...
  4. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
  5. Mazauric M, Dirick L, Purushothaman S, Bjork G, Lapeyre B. Trm112p is a 15-kDa zinc finger protein essential for the activity of two tRNA and one protein methyltransferases in yeast. J Biol Chem. 2010;285:18505-15 pubmed publisher
    ..An mtq2-0 trm9-0 strain exhibits a synthetic growth defect, thus revealing the existence of an unexpected link between tRNA ..
  6. Fabrizio P, Hoon S, Shamalnasab M, Galbani A, Wei M, Giaever G, et al. Genome-wide screen in Saccharomyces cerevisiae identifies vacuolar protein sorting, autophagy, biosynthetic, and tRNA methylation genes involved in life span regulation. PLoS Genet. 2010;6:e1001024 pubmed publisher
    ..Among the genes whose deletion significantly extended life span are ACB1, CKA2, and TRM9, implicated in fatty acid transport and biosynthesis, cell signaling, and tRNA methylation, respectively...
  7. Doyle F, Leonardi A, Endres L, Tenenbaum S, Dedon P, Begley T. Gene- and genome-based analysis of significant codon patterns in yeast, rat and mice genomes with the CUT Codon UTilization tool. Methods. 2016;107:98-109 pubmed publisher
    ..g., AGA, GAA, AAG) linked to translational regulation by tRNA methyltransferase 9 (Trm9)...
  8. Liger D, Mora L, Lazar N, Figaro S, Henri J, Scrima N, et al. Mechanism of activation of methyltransferases involved in translation by the Trm112 'hub' protein. Nucleic Acids Res. 2011;39:6249-59 pubmed publisher
    ..Trm112 activates not only the Mtq2 catalytic subunit but also two other tRNA methyltransferases (Trm9 and Trm11)...
  9. Chen C, Huang B, Anderson J, Byström A. Unexpected accumulation of ncm(5)U and ncm(5)S(2) (U) in a trm9 mutant suggests an additional step in the synthesis of mcm(5)U and mcm(5)S(2)U. PLoS ONE. 2011;6:e20783 pubmed publisher
    ..a complex pathway, where the last step in formation of mcm(5) is a methyl esterification of cm(5) dependent on the Trm9 and Trm112 proteins...

More Information


  1. Patil A, Chan C, Dyavaiah M, Rooney J, Dedon P, Begley T. Translational infidelity-induced protein stress results from a deficiency in Trm9-catalyzed tRNA modifications. RNA Biol. 2012;9:990-1001 pubmed publisher
    ..We hypothesized that wobble uridine modifications catalyzed by tRNA methyltransferase 9 (Trm9) are essential for translational fidelity...
  2. Patil A, Dyavaiah M, Joseph F, Rooney J, Chan C, Dedon P, et al. Increased tRNA modification and gene-specific codon usage regulate cell cycle progression during the DNA damage response. Cell Cycle. 2012;11:3656-65 pubmed publisher
    ..Translation of RNR1 has been linked to the wobble uridine modifying enzyme tRNA methyltransferase 9 (Trm9)...
  3. Deng W, Babu I, Su D, Yin S, Begley T, Dedon P. Trm9-Catalyzed tRNA Modifications Regulate Global Protein Expression by Codon-Biased Translation. PLoS Genet. 2015;11:e1005706 pubmed publisher
    ..mcm5) and 5-methoxy-carbonyl-methyl-2-thio (mcm5s2) modifications catalyzed by tRNA methyltransferase 9 (Trm9) in tRNAArg(UCU) and tRNAGlu(UUC) and selective translation of proteins from genes enriched with their cognate ..