Gene Symbol: TPM2
Description: tropomyosin TPM2
Alias: tropomyosin TPM2
Species: Saccharomyces cerevisiae S288c
Products:     TPM2

Top Publications

  1. Drees B, Brown C, Barrell B, Bretscher A. Tropomyosin is essential in yeast, yet the TPM1 and TPM2 products perform distinct functions. J Cell Biol. 1995;128:383-92 pubmed
    ..analysis of chromosome IX of Saccharomyces cerevisiae revealed an open reading frame of 166 residues, designated TPM2, having 64.5% sequence identity to TPM1, that encodes the major form of tropomyosin in yeast...
  2. Pruyne D, Bretscher A. Polarization of cell growth in yeast. J Cell Sci. 2000;113 ( Pt 4):571-85 pubmed
    ..The dynamic arrangement of targeting and recycling provides flexibility for the precise control of morphogenesis. ..
  3. Irazoqui J, Howell A, Theesfeld C, Lew D. Opposing roles for actin in Cdc42p polarization. Mol Biol Cell. 2005;16:1296-304 pubmed
    ..In addition, we report that once a bud has formed, polarized Cdc42p becomes more resistant to dispersal, revealing an unexpected difference between unbudded and budded cells in the organization of the polarization site. ..
  4. Pruyne D, Gao L, Bi E, Bretscher A. Stable and dynamic axes of polarity use distinct formin isoforms in budding yeast. Mol Biol Cell. 2004;15:4971-89 pubmed
    ..By coupling different formin isoforms to unique cortical landmarks, yeast uses common cytoskeletal elements to maintain stable and dynamic axes in the same cell. ..
  5. Kadota J, Yamamoto T, Yoshiuchi S, Bi E, Tanaka K. Septin ring assembly requires concerted action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5329-45 pubmed
    ..Our results suggest that polarisome components and Cla4p are required for the initial assembly of the septin ring and that the actin cytoskeleton is involved in this process. ..
  6. Pruyne D, Schott D, Bretscher A. Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. J Cell Biol. 1998;143:1931-45 pubmed
    ..Therefore, actin cables respond to polarity cues independently of the overall distribution of cortical patches and are able to directly target the Myo2p-dependent delivery of secretory vesicles and polarization of growth. ..
  7. Winsor B, Schiebel E. Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast. 1997;13:399-434 pubmed
  8. Sheltzer J, Rose M. The class V myosin Myo2p is required for Fus2p transport and actin polarization during the yeast mating response. Mol Biol Cell. 2009;20:2909-19 pubmed publisher
    ..The different velocities, differential sensitivity to mutation and lack of colocalization suggest that Fus2p and Sec4p, another Myo2p cargo associated with exocytotic vesicles, reside predominantly on different cellular organelles. ..
  9. Maytum R, Hatch V, Konrad M, Lehman W, Geeves M. Ultra short yeast tropomyosins show novel myosin regulation. J Biol Chem. 2008;283:1902-10 pubmed
    ..By showing that Tm can compete directly with myosin for the same binding site these new Tms provide direct evidence for the steric blocking model. ..

More Information


  1. Hermann G, King E, Shaw J. The yeast gene, MDM20, is necessary for mitochondrial inheritance and organization of the actin cytoskeleton. J Cell Biol. 1997;137:141-53 pubmed
    ..Thus, Mdm20p and Tpm1p may act in vivo to establish molecular and functional heterogeneity of the actin cytoskeleton. ..
  2. Meshcheryakov V, Nitanai Y, Maytum R, Geeves M, Maeda Y. Crystallization and preliminary X-ray crystallographic analysis of full-length yeast tropomyosin 2 from Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64:528-30 pubmed publisher
    ..8, b = 49.9, c = 104.0 A, alpha = gamma = 90.0, beta = 124.0 degrees and two molecules in the asymmetric unit. A complete native X-ray diffraction data set was collected to 3.5 A resolution using synchrotron radiation. ..
  3. Boldogh I, Ramcharan S, Yang H, Pon L. A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division. Mol Biol Cell. 2004;15:3994-4002 pubmed
    ..Finally, destabilization of actin cables and the resulting delocalization of Myo2p from the bud tip had no significant effect on the accumulation of mitochondria in the bud tip. ..
  4. Gao X, Albert S, Tcheperegine S, Burd C, Gallwitz D, Bi E. The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol. 2003;162:635-46 pubmed
    ..Using a strain defective in polarized secretion and actin-patch organization, we showed that a change in actin-patch organization could be a consequence of the fusion of mistargeted vesicles with the plasma membrane. ..
  5. Karpova T, Reck Peterson S, Elkind N, Mooseker M, Novick P, Cooper J. Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae. Mol Biol Cell. 2000;11:1727-37 pubmed
    ..This effect is similar to that of latrunculin, suggesting that Myo2p interacts with filamentous actin. However, inhibition of Myo2p function by expression of its tail domain completely abolished growth. ..
  6. Gao L, Bretscher A. Analysis of unregulated formin activity reveals how yeast can balance F-actin assembly between different microfilament-based organizations. Mol Biol Cell. 2008;19:1474-84 pubmed publisher
    ..One class encodes proteins that promote the assembly of actin cables (TPM1, TPM2, and ABP140), suggesting that the lethality was rescued by turning disorganized filaments into functional cables...
  7. Fan X, Martin Brown S, Florens L, Li R. Intrinsic capability of budding yeast cofilin to promote turnover of tropomyosin-bound actin filaments. PLoS ONE. 2008;3:e3641 pubmed publisher
    ..Our results suggest that yeast cofilin has the intrinsic ability to promote actin cable turnover, and that the severing activity may rely on its ability to bind Tpm1. ..
  8. Kagami M, Toh e A, Matsui Y. SRO9, a multicopy suppressor of the bud growth defect in the Saccharomyces cerevisiae rho3-deficient cells, shows strong genetic interactions with tropomyosin genes, suggesting its role in organization of the actin cytoskeleton. Genetics. 1997;147:1003-16 pubmed
    ..We isolated TPM2, the other gene for tropomyosin, as a multicopy suppressor of a tpm1delta sro9delta double mutant...
  9. Yamamoto T, Mochida J, Kadota J, Takeda M, Bi E, Tanaka K. Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast. Mol Biol Cell. 2010;21:1237-52 pubmed publisher