TPK1

Summary

Gene Symbol: TPK1
Description: cAMP-dependent protein kinase catalytic subunit TPK1
Alias: PKA1, SRA3, cAMP-dependent protein kinase catalytic subunit TPK1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cannon J, Tatchell K. Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase. Mol Cell Biol. 1987;7:2653-63 pubmed
    Mutations in the SRA1 or SRA3 gene eliminate the requirement for either RAS gene (RAS1 or RAS2) in Saccharomyces cerevisiae. We cloned SRA1 and SRA3 and determined their DNA sequences...
  2. Toda T, Cameron S, Sass P, Zoller M, Wigler M. Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell. 1987;50:277-87 pubmed
    We have isolated three genes (TPK1, TPK2, and TPK3) from the yeast S. cerevisiae that encode the catalytic subunits of the cAMP-dependent protein kinase...
  3. Kim J, Johnston M. Two glucose-sensing pathways converge on Rgt1 to regulate expression of glucose transporter genes in Saccharomyces cerevisiae. J Biol Chem. 2006;281:26144-9 pubmed
    ..Thus, two different glucose signal transduction pathways converge on Rgt1 to regulate expression of glucose transporters. ..
  4. Griffioen G, Anghileri P, Imre E, Baroni M, Ruis H. Nutritional control of nucleocytoplasmic localization of cAMP-dependent protein kinase catalytic and regulatory subunits in Saccharomyces cerevisiae. J Biol Chem. 2000;275:1449-56 pubmed
  5. Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
    ..We conclude that cytosolic kinases exert stimulatory and inhibitory effects on biogenesis and function of the TOM complex and thus regulate protein import into mitochondria. ..
  6. Gelperin D, Weigle J, Nelson K, Roseboom P, Irie K, Matsumoto K, et al. 14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1995;92:11539-43 pubmed
    ..of BMH genes also partially suppresses the temperature sensitivity of the cdc25-1 mutant, and high-copy TPK1, encoding a cAMP-dependent protein kinase, restores Bmh- yeast to viability...
  7. Panni S, Landgraf C, Volkmer Engert R, Cesareni G, Castagnoli L. Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae. FEMS Yeast Res. 2008;8:53-63 pubmed
    ..This work represents the first demonstration of enzyme activation mediated by 14-3-3 binding in yeast. ..
  8. Robertson L, Fink G. The three yeast A kinases have specific signaling functions in pseudohyphal growth. Proc Natl Acad Sci U S A. 1998;95:13783-7 pubmed
    ..b>Tpk1 has no discernible effect...
  9. Veisova D, Macakova E, Rezabkova L, Sulc M, Vacha P, Sychrova H, et al. Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1. Biochem J. 2012;443:663-70 pubmed publisher

More Information

Publications78

  1. Kafadar K, Cyert M. Integration of stress responses: modulation of calcineurin signaling in Saccharomyces cerevisiae by protein kinase A. Eukaryot Cell. 2004;3:1147-53 pubmed
    ..Thus, these studies identify a specific biochemical mechanism through which the activities of multiple stress-activated signaling pathways are integrated in vivo. ..
  2. Popova Y, Thayumanavan P, Lonati E, Agrochão M, Thevelein J. Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor. Proc Natl Acad Sci U S A. 2010;107:2890-5 pubmed publisher
    ..Our results provide to the best of our knowledge the first insight into the molecular mechanism of a phosphate transceptor. ..
  3. Leadsham J, Gourlay C. cAMP/PKA signaling balances respiratory activity with mitochondria dependent apoptosis via transcriptional regulation. BMC Cell Biol. 2010;11:92 pubmed publisher
    ..The visualization of cAMP/TPK3 induced cell death within yeast colonies supports a model that PKA regulation plays a physiological role in coordinating respiratory function and cell death with nutritional status in budding yeast. ..
  4. Makanae K, Kintaka R, Makino T, Kitano H, Moriya H. Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method. Genome Res. 2013;23:300-11 pubmed publisher
    ..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition. ..
  5. Martin Montalvo A, González Mariscal I, Padilla S, Ballesteros M, Brautigan D, Navas P, et al. Respiratory-induced coenzyme Q biosynthesis is regulated by a phosphorylation cycle of Cat5p/Coq7p. Biochem J. 2011;440:107-14 pubmed publisher
    ..These modifications produced in Coq7p also alter the ratio between DMQ(6) and CoQ(6) itself, indicating that the Coq7p phosphorylation state is a regulatory mechanism for CoQ(6) synthesis. ..
  6. Dong J, Bai X. The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae. FEBS Lett. 2011;585:1127-34 pubmed publisher
    ..These results suggest that Ras2p localization and Ras2-GEF activity of Cdc25p play roles in the feedback regulation of Ras2p in the Ras-cAMP pathway. ..
  7. Bolte M, Dieckhoff P, Krause C, Braus G, Irniger S. Synergistic inhibition of APC/C by glucose and activated Ras proteins can be mediated by each of the Tpk1-3 proteins in Saccharomyces cerevisiae. Microbiology. 2003;149:1205-16 pubmed
    ..Furthermore, it is shown that the three PKAs in yeast, Tpk1, Tpk2 and Tpk3, have redundant functions in regulating APC/C in response to glucose medium...
  8. Lusk C, Waller D, Makhnevych T, Dienemann A, Whiteway M, Thomas D, et al. Nup53p is a target of two mitotic kinases, Cdk1p and Hrr25p. Traffic. 2007;8:647-60 pubmed
    ..The ability of nup53 alleles containing Cdk1p site mutations to complement synthetic defects of nup53 Delta nup170 Delta strains is linked to a function for Nup53p in the spindle assembly checkpoint. ..
  9. Portela P, Moreno S. Glucose-dependent activation of protein kinase A activity in Saccharomyces cerevisiae and phosphorylation of its TPK1 catalytic subunit. Cell Signal. 2006;18:1072-86 pubmed
    ..Reversible phosphorylation can thus be considered a new control mechanism possibly pointing to a fine-tuning of PKA activity in response to environmental conditions. ..
  10. Banerjee D, Carrasquillo E, Hughey P, Schutzbach J, Martinez J, Baksi K. In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase. J Biol Chem. 2005;280:4174-81 pubmed
    ..Thus, confirming that S. cerevisiae DPMS activity is indeed regulated by the cAMP-dependent protein phosphorylation signal, and the phosphorylation target is serine 141. ..
  11. Su W, Han G, Casciano J, Carman G. Protein kinase A-mediated phosphorylation of Pah1p phosphatidate phosphatase functions in conjunction with the Pho85p-Pho80p and Cdc28p-cyclin B kinases to regulate lipid synthesis in yeast. J Biol Chem. 2012;287:33364-76 pubmed publisher
  12. Solari C, Tudisca V, Pugliessi M, Nadra A, Moreno S, Portela P. Regulation of PKA activity by an autophosphorylation mechanism in Saccharomyces cerevisiae. Biochem J. 2014;462:567-79 pubmed publisher
    ..the PKA regulatory subunit is encoded by the gene BCY1, and the catalytic subunits are encoded by three genes: TPK1, TPK2 and TPK3...
  13. Portela P, Howell S, Moreno S, Rossi S. In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A. J Biol Chem. 2002;277:30477-87 pubmed
    ..kinase 1 (Pyk1) was demonstrated to be associated to an immunoprecipitate of yeast protein kinase A holoenzyme (HA-Tpk1.Bcy1) and to be phosphorylated in a cAMP-dependent process...
  14. Rayner T, Gray J, Thorner J. Direct and novel regulation of cAMP-dependent protein kinase by Mck1p, a yeast glycogen synthase kinase-3. J Biol Chem. 2002;277:16814-22 pubmed
  15. Stephan J, Yeh Y, Ramachandran V, Deminoff S, Herman P. The Tor and PKA signaling pathways independently target the Atg1/Atg13 protein kinase complex to control autophagy. Proc Natl Acad Sci U S A. 2009;106:17049-54 pubmed publisher
    ..In all, our data indicate that the PKA and Tor pathways function independently to control autophagy in S. cerevisiae, and that the Atg1/Atg13 kinase complex is a key site of signal integration within this degradative pathway. ..
  16. Peeters T, Louwet W, Geladé R, Nauwelaers D, Thevelein J, Versele M. Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast. Proc Natl Acad Sci U S A. 2006;103:13034-9 pubmed
    ..Importantly, we show that Krh1/2 also enhance the association between mouse R and C subunits, suggesting that Krh control of PKA has been evolutionarily conserved. ..
  17. Kang W, Kim Y, Kang H, Kwon K, Kim J. Sir2 phosphorylation through cAMP-PKA and CK2 signaling inhibits the lifespan extension activity of Sir2 in yeast. elife. 2015;4: pubmed publisher
    ..Our results demonstrate a mechanism by which Sir2 contributes to lifespan extension. ..
  18. Moretto F, van Werven F. Transcription of the mating-type-regulated lncRNA IRT1 is governed by TORC1 and PKA. Curr Genet. 2017;63:325-329 pubmed publisher
    ..Our results indicate that there is a hierarchy between nutrient and mating-type signals in controlling the decision to enter sporulation. ..
  19. Marash M, Gerst J. Phosphorylation of the autoinhibitory domain of the Sso t-SNAREs promotes binding of the Vsm1 SNARE regulator in yeast. Mol Biol Cell. 2003;14:3114-25 pubmed
    ..Thus, one way by which phosphorylation inhibits SNARE assembly could be by regulating the association of inhibitory factors that control the ability of t-SNAREs to form complexes in vivo. ..
  20. Ramachandran V, Shah K, Herman P. The cAMP-dependent protein kinase signaling pathway is a key regulator of P body foci formation. Mol Cell. 2011;43:973-81 pubmed publisher
    ..This work therefore highlights the general relevance of RNP foci in quiescent cells, and provides a framework for the study of the many RNP assemblies that form in eukaryotic cells. ..
  21. Weidberg H, Moretto F, Spedale G, Amon A, van Werven F. Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability. PLoS Genet. 2016;12:e1006075 pubmed publisher
    ..Collectively our data demonstrate that nutrient control of entry into sporulation is mediated by a combination of energy availability, TORC1 and PKA activities that converge on the IME1 promoter. ..
  22. Garrett S, Broach J. Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase. Genes Dev. 1989;3:1336-48 pubmed
    ..in vitro reveal that it is not essential for growth and that its loss confers growth to a strain deleted for tpk1, tpk2, and tpk3, the structural genes for the catalytic subunit of the cAMP-dependent protein kinase...
  23. Gerbeth C, Schmidt O, Rao S, Harbauer A, Mikropoulou D, Opalińska M, et al. Glucose-induced regulation of protein import receptor Tom22 by cytosolic and mitochondria-bound kinases. Cell Metab. 2013;18:578-87 pubmed publisher
    ..Our results reveal that three kinases regulate the import and assembly of Tom22, demonstrating that the central receptor is a major target for the posttranslational regulation of mitochondrial protein import. ..
  24. Powers S, O Neill K, Wigler M. Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae. Mol Cell Biol. 1989;9:390-5 pubmed
    ..These results suggest that these mutant RAS genes interfere with the normal interaction of RAS and CDC25 proteins and suggest that this interaction is direct and has evolutionarily conserved features. ..
  25. Trott A, Shaner L, Morano K. The molecular chaperone Sse1 and the growth control protein kinase Sch9 collaborate to regulate protein kinase A activity in Saccharomyces cerevisiae. Genetics. 2005;170:1009-21 pubmed
    ..Together these results demonstrate that the Sse1 chaperone and the growth control kinase Sch9 independently contribute to regulation of PKA signaling. ..
  26. Legesse Miller A, Zhang S, Santiago Tirado F, Van Pelt C, Bretscher A. Regulated phosphorylation of budding yeast's essential myosin V heavy chain, Myo2p. Mol Biol Cell. 2006;17:1812-21 pubmed
    ..These results suggest that in yeast, Myo2p is subject to phosphoregulation involving a PKA-related signaling pathway. ..
  27. Baccarini L, Martínez Montañés F, Rossi S, Proft M, Portela P. PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae. Biochim Biophys Acta. 2015;1849:1329-39 pubmed publisher
    ..There are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1...
  28. Mösch H, Kübler E, Krappmann S, Fink G, Braus G. Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1325-35 pubmed
  29. McCartney R, Garnar Wortzel L, Chandrashekarappa D, Schmidt M. Activation and inhibition of Snf1 kinase activity by phosphorylation within the activation loop. Biochim Biophys Acta. 2016;1864:1518-28 pubmed publisher
    ..The first is through direct dephosphorylation of the conserved activation loop threonine. The second is through phosphorylation of serine 214. ..
  30. Santhanam A, Hartley A, Düvel K, Broach J, Garrett S. PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p. Eukaryot Cell. 2004;3:1261-71 pubmed
    ..Finally, Msn2p localization is unaffected by conditional loss of 14-3-3 protein function, ruling out the possibility that 14-3-3 proteins act as a scaffold to sequester Msn2p in the cytoplasm. ..
  31. Chen R, Thorner J. Systematic epistasis analysis of the contributions of protein kinase A- and mitogen-activated protein kinase-dependent signaling to nutrient limitation-evoked responses in the yeast Saccharomyces cerevisiae. Genetics. 2010;185:855-70 pubmed publisher
    ..Thus, although there are similarities between haploids and diploids, cell type-specific differences clearly alter the balance of the signaling inputs required to elicit the various nutrient limitation-evoked cellular behaviors. ..
  32. Grandin N, Charbonneau M. Budding yeast 14-3-3 proteins contribute to the robustness of the DNA damage and spindle checkpoints. Cell Cycle. 2008;7:2749-61 pubmed
    ..Therefore, the budding yeast 14-3-3 proteins contribute to the robustness of the two major mitotic checkpoints and, by doing so, may also ensure optimal coordination between the responses to two distinct types of damage. ..
  33. Graef M, Nunnari J. Mitochondria regulate autophagy by conserved signalling pathways. EMBO J. 2011;30:2101-14 pubmed publisher
    ..Our findings indicate an interrelation of two common risk factors-mitochondrial dysfunction and autophagy inhibition-for ageing, cancerogenesis, and neurodegeneration. ..
  34. Ward M, Garrett S. Suppression of a yeast cyclic AMP-dependent protein kinase defect by overexpression of SOK1, a yeast gene exhibiting sequence similarity to a developmentally regulated mouse gene. Mol Cell Biol. 1994;14:5619-27 pubmed
    ..Overexpression of SOK1, like lesions in YAK1, also restores growth to a strain (tpk1 tpk2 tpk3) lacking all A kinase activity...
  35. Rao S, Schmidt O, Harbauer A, Sch nfisch B, Guiard B, Pfanner N, et al. Biogenesis of the preprotein translocase of the outer mitochondrial membrane: protein kinase A phosphorylates the precursor of Tom40 and impairs its import. Mol Biol Cell. 2012;23:1618-27 pubmed publisher
    ..We conclude that PKA plays a dual role in the regulation of the TOM complex. Phosphorylation by PKA not only impairs the receptor activity of Tom70, but it also inhibits the biogenesis of the channel protein Tom40...
  36. Hartley A, Ward M, Garrett S. The Yak1 protein kinase of Saccharomyces cerevisiae moderates thermotolerance and inhibits growth by an Sch9 protein kinase-independent mechanism. Genetics. 1994;136:465-74 pubmed
    ..These results, for the first time, associate a phenotype, other than suppression of the A kinase growth defect, with the loss of Yak1 activity and argue a broader role for the Yak1 kinase in cell growth. ..
  37. Lenssen E, Oberholzer U, Labarre J, De Virgilio C, Collart M. Saccharomyces cerevisiae Ccr4-not complex contributes to the control of Msn2p-dependent transcription by the Ras/cAMP pathway. Mol Microbiol. 2002;43:1023-37 pubmed
    ..Together, our results suggest that the Ccr4-Not complex may function as an effector of the Ras/cAMP pathway that contributes to repress basal, stress- and starvation-induced transcription by Msn2/4p. ..
  38. Hartley A, Bogaerts S, Garrett S. cAMP inhibits bud growth in a yeast strain compromised for Ca2+ influx into the Golgi. Mol Gen Genet. 1996;251:556-64 pubmed
    ..One candidate for such a protein is the cAMP-binding membrane ectoprotein recently described in yeast. ..
  39. Powers S, Gonzales E, Christensen T, Cubert J, Broek D. Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant. Cell. 1991;65:1225-31 pubmed
    ..We propose that BUD5 is a member of a family of CDC25-related genes that encode activators of RAS and RAS-like proteins. ..
  40. Voordeckers K, Kimpe M, Haesendonckx S, Louwet W, Versele M, Thevelein J. Yeast 3-phosphoinositide-dependent protein kinase-1 (PDK1) orthologs Pkh1-3 differentially regulate phosphorylation of protein kinase A (PKA) and the protein kinase B (PKB)/S6K ortholog Sch9. J Biol Chem. 2011;286:22017-27 pubmed publisher
    ..Mutagenesis of the PDK1 site in Tpk1 abolished binding of the regulatory subunit and cAMP dependence...
  41. Gurunathan S, Marash M, Weinberger A, Gerst J. t-SNARE phosphorylation regulates endocytosis in yeast. Mol Biol Cell. 2002;13:1594-607 pubmed
    ..These results suggest that endocytosis is also modulated by t-SNARE phosphorylation in vivo. ..
  42. Deminoff S, Ramachandran V, Herman P. Distal recognition sites in substrates are required for efficient phosphorylation by the cAMP-dependent protein kinase. Genetics. 2009;182:529-39 pubmed publisher
  43. Prusty R, Keil R. SCH9, a putative protein kinase from Saccharomyces cerevisiae, affects HOT1-stimulated recombination. Mol Genet Genomics. 2004;272:264-74 pubmed
    ..Thus, transcription is necessary but not sufficient for HOT1 activity. TPK1, which encodes a catalytic subunit of PKA, is a multicopy suppressor of the recombination and growth defects of ..
  44. Bavli Kertselli I, Melamed D, Bar Ziv L, Volf H, Arava Y. Overexpression of eukaryotic initiation factor 5 rescues the translational defect of tpk1w in a manner that necessitates a novel phosphorylation site. FEBS J. 2015;282:504-20 pubmed publisher
    ..This translation factor is as yet unknown. A cAMP-dependent protein kinase mutant yeast strain (tpk1(w)) that does not respond properly to glucose depletion and maintains translation was described previously...
  45. Labedzka K, Tian C, Nussbaumer U, Timmermann S, Walther P, Müller J, et al. Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. J Cell Sci. 2012;125:4103-13 pubmed publisher
    ..Owing to the overlapping binding specificities of its members the HICS complex is best described as ensembles of isomeric interaction states that precisely coordinate the different functions of the interactors during cytokinesis. ..
  46. Searle J, Schollaert K, Wilkins B, Sanchez Y. The DNA damage checkpoint and PKA pathways converge on APC substrates and Cdc20 to regulate mitotic progression. Nat Cell Biol. 2004;6:138-45 pubmed
    ..sites after DNA damage, and this phosphorylation requires the Atr orthologue Mec1 and the PKA catalytic subunits Tpk1 and Tpk2...
  47. Galello F, Portela P, Moreno S, Rossi S. Characterization of substrates that have a differential effect on Saccharomyces cerevisiae protein kinase A holoenzyme activation. J Biol Chem. 2010;285:29770-9 pubmed publisher
    ..The catalytic turnover numbers of the catalytic subunits isoforms Tpk1 and Tpk2 were determined, and both enzymes are shown to have the same value of 3 s(-1)...
  48. Kimpe M, Voordeckers K, Thevelein J, Van Zeebroeck G. Pkh1 interacts with and phosphorylates components of the yeast Gcn2/eIF2? system. Biochem Biophys Res Commun. 2012;419:89-94 pubmed publisher
    ..Hence, the physiological importance of the close interactions between Pkh1 and Gcn2 or eIF2 could depend on other conditions and/or other targets of the Gcn2/eIF2 system. ..
  49. Park J, Grant C, Dawes I. The high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae is the major determinant of cAMP levels in stationary phase: involvement of different branches of the Ras-cyclic AMP pathway in stress responses. Biochem Biophys Res Commun. 2005;327:311-9 pubmed
  50. Schmelzle T, Beck T, Martin D, Hall M. Activation of the RAS/cyclic AMP pathway suppresses a TOR deficiency in yeast. Mol Cell Biol. 2004;24:338-51 pubmed
    ..Finally, and importantly, TOR controls the subcellular localization of both the protein kinase A catalytic subunit TPK1 and the RAS/cAMP signaling-related kinase YAK1...
  51. Gancedo J, Flores C, Gancedo C. The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae. Biochim Biophys Acta. 2015;1850:1362-7 pubmed publisher
    ..Repression is dependent on PKA activity, but not on any specific Tpk isoenzyme. The results show that previously overlooked regulatory elements, such as Rgt1 and Tpks, participate in the control of SUC2 expression in S.cerevisiae. ..
  52. Zurita Martinez S, Cardenas M. Tor and cyclic AMP-protein kinase A: two parallel pathways regulating expression of genes required for cell growth. Eukaryot Cell. 2005;4:63-71 pubmed
  53. Fujimura K, Tanaka K, Toh e A. A dominant interfering mutation in RAS1 of Saccharomyces cerevisiae. Mol Gen Genet. 1993;241:280-6 pubmed
    ..These results suggest that the RAS1Ser22 gene product interferes with the normal interaction of Ras with Cdc25 by forming a dead-end complex between Ras1Ser22 and Cdc25 proteins. ..
  54. Budovskaya Y, Stephan J, Reggiori F, Klionsky D, Herman P. The Ras/cAMP-dependent protein kinase signaling pathway regulates an early step of the autophagy process in Saccharomyces cerevisiae. J Biol Chem. 2004;279:20663-71 pubmed
    ..Finally, the data suggest that at least a portion of the Ras/PKA effects on stationary phase survival are the result of the regulation of autophagy activity by this signaling pathway. ..
  55. Schneper L, Krauss A, Miyamoto R, Fang S, Broach J. The Ras/protein kinase A pathway acts in parallel with the Mob2/Cbk1 pathway to effect cell cycle progression and proper bud site selection. Eukaryot Cell. 2004;3:108-20 pubmed
    ..the double-deletion strains are Ace2 independent but are suppressed by overexpression of the PKA catalytic subunit, Tpk1. From these observations, we conclude that the PKA pathway and Mob2/Cbk1 act in parallel to determine bud site ..
  56. Zähringer H, Holzer H, Nwaka S. Stability of neutral trehalase during heat stress in Saccharomyces cerevisiae is dependent on the activity of the catalytic subunits of cAMP-dependent protein kinase, Tpk1 and Tpk2. Eur J Biochem. 1998;255:544-51 pubmed
    ..Introduction of a plasmid carrying the TPK1 or TPK2 gene into tpk1tpk2TPK3 cells restores the heat-induced increase of neutral trehalase activity...
  57. Marash M, Gerst J. t-SNARE dephosphorylation promotes SNARE assembly and exocytosis in yeast. EMBO J. 2001;20:411-21 pubmed
    ..Thus, modulation of t-SNARE phosphorylation regulates SNARE complex assembly and membrane fusion in vivo. ..
  58. Roy A, Shin Y, Cho K, Kim J. Mth1 regulates the interaction between the Rgt1 repressor and the Ssn6-Tup1 corepressor complex by modulating PKA-dependent phosphorylation of Rgt1. Mol Biol Cell. 2013;24:1493-503 pubmed publisher
    ..Taken together, these findings show that Mth1 is a transcriptional corepressor that facilitates the recruitment of Ssn6-Tup1 by Rgt1. ..
  59. Demlow C, Fox T. Activity of mitochondrially synthesized reporter proteins is lower than that of imported proteins and is increased by lowering cAMP in glucose-grown Saccharomyces cerevisiae cells. Genetics. 2003;165:961-74 pubmed
    ..The resulting signal was transduced through redundant action of the three cAMP-dependent protein kinases, TPK1, TPK2, and TPK3...
  60. Park J, Collinson E, Grant C, Dawes I. Rom2p, the Rho1 GTP/GDP exchange factor of Saccharomyces cerevisiae, can mediate stress responses via the Ras-cAMP pathway. J Biol Chem. 2005;280:2529-35 pubmed
  61. Sadeh A, Movshovich N, Volokh M, Gheber L, Aharoni A. Fine-tuning of the Msn2/4-mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners. Mol Biol Cell. 2011;22:3127-38 pubmed publisher
    ..Such negative regulation is crucial to minimize the cost of uncontrolled stress response gene expression and ensures a high growth rate in the absence of stress. ..
  62. Schepers W, Van Zeebroeck G, Pinkse M, Verhaert P, Thevelein J. In vivo phosphorylation of Ser21 and Ser83 during nutrient-induced activation of the yeast protein kinase A (PKA) target trehalase. J Biol Chem. 2012;287:44130-42 pubmed publisher
    ..Our results reveal that trehalase activation in vivo is associated with phosphorylation of typical PKA sites and thus establish the enzyme as a reliable read-out for nutrient activation of PKA in vivo...
  63. Mizunuma M, Tsubakiyama R, Ogawa T, Shitamukai A, Kobayashi Y, Inai T, et al. Ras/cAMP-dependent protein kinase (PKA) regulates multiple aspects of cellular events by phosphorylating the Whi3 cell cycle regulator in budding yeast. J Biol Chem. 2013;288:10558-66 pubmed publisher
    ..Thus, PKA modulated the function of Whi3 by phosphorylation, thus implicating PKA-mediated modulation of Whi3 in multiple cellular events. ..
  64. Moir R, Lee J, Willis I. Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation. J Biol Chem. 2012;287:30833-41 pubmed publisher
    ..Together, these data do not support the proposed requirement for CK2 phosphorylation of Maf1 during derepression of pol III transcription...
  65. Fabret C, Cosnier B, Lekomtsev S, Gillet S, Hatin I, Le Marechal P, et al. A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability. BMC Mol Biol. 2008;9:22 pubmed publisher
    ..They also raise interesting questions about the relation between GTPase activity of Sup35 and its essential function in yeast. ..
  66. Haesendonckx S, Tudisca V, Voordeckers K, Moreno S, Thevelein J, Portela P. The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae. Biochem J. 2012;448:307-20 pubmed publisher
    ..Saccharomyces cerevisiae contains three PKA catalytic subunits, TPK1, TPK2 and TPK3...
  67. Casado C, González A, Platara M, Ruiz A, Arino J. The role of the protein kinase A pathway in the response to alkaline pH stress in yeast. Biochem J. 2011;438:523-33 pubmed publisher
    ..However, the relevance of attenuation of PKA in high pH tolerance is probably not restricted to regulation of Msn2 function. ..
  68. Lee P, Paik S, Shin C, Huh W, Hahn J. Regulation of yeast Yak1 kinase by PKA and autophosphorylation-dependent 14-3-3 binding. Mol Microbiol. 2011;79:633-46 pubmed publisher
    ..Since the binding of 14-3-3 proteins to Yak1 coincides with PKA activity, such regulatory mechanisms might allow cytoplasmic retention of an inactive form of Yak1 under high glucose conditions. ..
  69. Reinders A, Bürckert N, Boller T, Wiemken A, De Virgilio C. Saccharomyces cerevisiae cAMP-dependent protein kinase controls entry into stationary phase through the Rim15p protein kinase. Genes Dev. 1998;12:2943-55 pubmed
    ..Taken together, these results place Rim15p immediately downstream and under negative control of cAPK and define a positive regulatory role of Rim15p for entry into both meiosis and stationary phase. ..