TOM22

Summary

Gene Symbol: TOM22
Description: Tom22p
Alias: MAS17, MAS22, MOM22, Tom22p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Qiu J, Wenz L, Zerbes R, Oeljeklaus S, Bohnert M, Stroud D, et al. Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. Cell. 2013;154:596-608 pubmed publisher
    ..barrel formation was inhibited by SAM mutants and, unexpectedly, by mutants of the central import receptor, Tom22. We show that the cytosolic domain of Tom22 links TOM and SAM into a supercomplex, facilitating precursor transfer ..
  2. Chacinska A, van der Laan M, Mehnert C, Guiard B, Mick D, Hutu D, et al. Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting. Mol Cell Biol. 2010;30:307-18 pubmed publisher
    ..Depending on the signals of the preproteins, switches between the different forms of supercomplex and TIM23 are required for the completion of preprotein import. ..
  3. van Wilpe S, Ryan M, Hill K, Maarse A, Meisinger C, Brix J, et al. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature. 1999;401:485-9 pubmed
    ..The central receptor Tom22 binds preproteins through both its cytosolic domain and its intermembrane space domain and is stably associated ..
  4. Hönlinger A, Kubrich M, Moczko M, Gartner F, Mallet L, Bussereau F, et al. The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins. Mol Cell Biol. 1995;15:3382-9 pubmed
    ..This receptor complex contains two import receptors, a general insertion pore and the protein Mom22. It was unknown if Mom22 directly interacts with preproteins, and two views existed about the possible functions ..
  5. Böttinger L, Gornicka A, Czerwik T, Bragoszewski P, Loniewska Lwowska A, Schulze Specking A, et al. In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins. Mol Biol Cell. 2012;23:3957-69 pubmed publisher
    ..Thus Mia40 in cooperation with Erv1 promotes the formation of two disulfide bonds in the substrate protein, ensuring the efficiency of oxidative folding in the intermembrane space of mitochondria. ..
  6. Stroud D, Becker T, Qiu J, Stojanovski D, Pfannschmidt S, Wirth C, et al. Biogenesis of mitochondrial ?-barrel proteins: the POTRA domain is involved in precursor release from the SAM complex. Mol Biol Cell. 2011;22:2823-33 pubmed publisher
    ..These results indicate the POTRA domain of Sam50 is not essential for recognition of ?-barrel precursors but functions in a subsequent step to promote the release of precursor proteins from the SAM complex. ..
  7. Yamano K, Yatsukawa Y, Esaki M, Hobbs A, Jensen R, Endo T. Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. J Biol Chem. 2008;283:3799-807 pubmed
    Precise targeting of mitochondrial precursor proteins to mitochondria requires receptor functions of Tom20, Tom22, and Tom70 on the mitochondrial surface...
  8. Model K, Meisinger C, Prinz T, Wiedemann N, Truscott K, Pfanner N, et al. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001;8:361-70 pubmed
    ..The precursor of the channel-lining Tom40 is first targeted to the membrane via the receptor proteins Tom20 and Tom22; it then assembles with Tom5 to form the 250 kDa intermediate exposed to the intermembrane space...
  9. Dekker P, Ryan M, Brix J, Muller H, Hönlinger A, Pfanner N. Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol Cell Biol. 1998;18:6515-24 pubmed
    ..We have analyzed the molecular architecture of the Tom machinery. The receptor Tom22 stably associates with Tom40, the main component of the GIP, in a complex with a molecular weight of approximately ..

More Information

Publications46

  1. Becker T, Wenz L, Thornton N, Stroud D, Meisinger C, Wiedemann N, et al. Biogenesis of mitochondria: dual role of Tom7 in modulating assembly of the preprotein translocase of the outer membrane. J Mol Biol. 2011;405:113-24 pubmed publisher
    ..Since the SAM-Mdm10 complex is required for the biogenesis of Tom22, Tom7 delays the assembly of Tom22 with Tom40 at a late stage of assembly of the TOM complex...
  2. Wiedemann N, Kozjak V, Prinz T, Ryan M, Meisinger C, Pfanner N, et al. Biogenesis of yeast mitochondrial cytochrome c: a unique relationship to the TOM machinery. J Mol Biol. 2003;327:465-74 pubmed
    ..Mitochondria lacking the central receptor and organizing protein Tom22 contain greatly reduced levels of cytochrome c...
  3. Wrobel L, Trojanowska A, Sztolsztener M, Chacinska A. Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria. Mol Biol Cell. 2013;24:543-54 pubmed publisher
    ..We propose that Mia40 not only is responsible for disulfide bond formation, but also assists the Tim22 protein in its integration into the inner membrane of mitochondria. ..
  4. Becker T, Wenz L, Krüger V, Lehmann W, Müller J, Goroncy L, et al. The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins. J Cell Biol. 2011;194:387-95 pubmed publisher
    ..We conclude that the Mim1 complex plays a central role in the import of ?-helical outer membrane proteins with multiple transmembrane segments. ..
  5. Schulz C, Rehling P. Remodelling of the active presequence translocase drives motor-dependent mitochondrial protein translocation. Nat Commun. 2014;5:4349 pubmed publisher
    ..Thus, a replenishment cycle of co-chaperones at the TIM23 complex is an integral part of Hsp70's ATPase cycle at the channel exit site and essential to maintain motor-driven mitochondrial protein import. ..
  6. Becker T, Pfannschmidt S, Guiard B, Stojanovski D, Milenkovic D, Kutik S, et al. Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors. J Biol Chem. 2008;283:120-7 pubmed
    ..The three receptors Tom20, Tom22, and Tom70 are anchored in the outer membrane by a single transmembrane alpha-helix, located at the N terminus in ..
  7. Yamano K, Tanaka Yamano S, Endo T. Tom7 regulates Mdm10-mediated assembly of the mitochondrial import channel protein Tom40. J Biol Chem. 2010;285:41222-31 pubmed publisher
    ..These results collectively show that Tom7 recruits Mdm10, enhancing its association with the MMM1 complex, to regulate timing of the release of Tom40 from the TOB complex for subsequent assembly into the TOM40 complex. ..
  8. Chacinska A, Rehling P, Guiard B, Frazier A, Schulze Specking A, Pfanner N, et al. Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J. 2003;22:5370-81 pubmed
    ..The IMS domain of the receptor Tom22 is required for stabilization of the translocation contact site supercomplex...
  9. Zerbes R, Bohnert M, Stroud D, von der Malsburg K, Kram A, Oeljeklaus S, et al. Role of MINOS in mitochondrial membrane architecture: cristae morphology and outer membrane interactions differentially depend on mitofilin domains. J Mol Biol. 2012;422:183-91 pubmed publisher
  10. Burri L, Vascotto K, Gentle I, Chan N, Beilharz T, Stapleton D, et al. Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae. FEBS J. 2006;273:1507-15 pubmed
    ..The alpha-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane. ..
  11. Bohnert M, Wenz L, Zerbes R, Horvath S, Stroud D, von der Malsburg K, et al. Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane. Mol Biol Cell. 2012;23:3948-56 pubmed publisher
    ..We conclude that MINOS interacts with TOM and SAM independently and that the core subunit mitofilin is involved in biogenesis of outer membrane ?-barrel proteins. ..
  12. Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
    ..CK2 promotes biogenesis of the TOM complex by phosphorylation of two key components, the receptor Tom22 and the import protein Mim1, which in turn are required for import of further Tom proteins...
  13. Yamamoto H, Itoh N, Kawano S, Yatsukawa Y, Momose T, Makio T, et al. Dual role of the receptor Tom20 in specificity and efficiency of protein import into mitochondria. Proc Natl Acad Sci U S A. 2011;108:91-6 pubmed publisher
    ..Therefore Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency...
  14. Mokranjac D, Popov Celeketic D, Hell K, Neupert W. Role of Tim21 in mitochondrial translocation contact sites. J Biol Chem. 2005;280:23437-40 pubmed
    ..We propose that Tim21 binds to the trans site of the TOM complex thus keeping the two translocases in close contact. ..
  15. Esaki M, Shimizu H, Ono T, Yamamoto H, Kanamori T, Nishikawa S, et al. Mitochondrial protein import. Requirement of presequence elements and tom components for precursor binding to the TOM complex. J Biol Chem. 2004;279:45701-7 pubmed
    ..Tom7 constitutes or is close to the trans site and has overlapping functions with the C-terminal intermembrane space domain of Tom22 in the mitochondrial protein import.
  16. Model K, Prinz T, Ruiz T, Radermacher M, Krimmer T, Kuhlbrandt W, et al. Protein translocase of the outer mitochondrial membrane: role of import receptors in the structural organization of the TOM complex. J Mol Biol. 2002;316:657-66 pubmed
    ..This translocase of the outer membrane (TOM) consists of three receptor proteins, Tom20, Tom22 and Tom70, the channel protein Tom40, and several small Tom proteins...
  17. SCHULKE N, Sepuri N, Gordon D, Saxena S, Dancis A, Pain D. A multisubunit complex of outer and inner mitochondrial membrane protein translocases stabilized in vivo by translocation intermediates. J Biol Chem. 1999;274:22847-54 pubmed
    ..contains all Tom and Tim components known to be essential for import of matrix-targeted proteins, namely Tom40, Tom22, Tim17, Tim23, Tim44, and matrix-localized Hsp70...
  18. Haucke V, Horst M, Schatz G, Lithgow T. The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor. EMBO J. 1996;15:1231-7 pubmed
    ..We conclude that the two receptor subcomplexes of the mitochondrial protein import receptor interact in vivo via their Mas20p and Mas70p subunits and that this interaction is functionally important. ..
  19. Shiota T, Mabuchi H, Tanaka Yamano S, Yamano K, Endo T. In vivo protein-interaction mapping of a mitochondrial translocator protein Tom22 at work. Proc Natl Acad Sci U S A. 2011;108:15179-83 pubmed publisher
    ..Here we analyze the interactions of Tom22, a multifunctional subunit of the outer membrane translocator TOM40 complex, with other translocator subunits such ..
  20. Horvath S, Böttinger L, Vögtle F, Wiedemann N, Meisinger C, Becker T, et al. Processing and topology of the yeast mitochondrial phosphatidylserine decarboxylase 1. J Biol Chem. 2012;287:36744-55 pubmed publisher
    ..In summary, membrane integration of Psd1 is crucial for its functionality and for maintenance of mitochondrial lipid homeostasis. ..
  21. Court D, Nargang F, Steiner H, Hodges R, Neupert W, Lill R. Role of the intermembrane-space domain of the preprotein receptor Tom22 in protein import into mitochondria. Mol Cell Biol. 1996;16:4035-42 pubmed
    b>Tom22 is an essential component of the protein translocation complex (Tom complex) of the mitochondrial outer membrane. The N-terminal domain of Tom22 functions as a preprotein receptor in cooperation with Tom20...
  22. Moczko M, Dietmeier K, Sollner T, Segui B, Steger H, Neupert W, et al. Identification of the mitochondrial receptor complex in Saccharomyces cerevisiae. FEBS Lett. 1992;310:265-8 pubmed
    ..MOM72, were found in a complex with the general insertion protein, GIP (formed by MOM7, MOM8, MOM30 and MOM38) and MOM22. We isolated a complex out of S...
  23. Dietmeier K, Hönlinger A, Bömer U, Dekker P, Eckerskorn C, Lottspeich F, et al. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature. 1997;388:195-200 pubmed
    ..Tom20-Tom22 and Tom70-Tom37 function as import receptors with a preference for preproteins that have amino-terminal ..
  24. Albrecht R, Rehling P, Chacinska A, Brix J, Cadamuro S, Volkmer R, et al. The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes. EMBO Rep. 2006;7:1233-8 pubmed
    ..space, directly connects the TOM and TIM23 complexes by binding to the intermembrane space domain of the Tom22 receptor...
  25. Chacinska A, Lind M, Frazier A, Dudek J, Meisinger C, Geissler A, et al. Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell. 2005;120:817-29 pubmed
    ..Thus, the presequence translocase is not a static complex but switches between TOM tethering and PAM binding in a reaction cycle involving Tim21 and Tim17. ..
  26. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..
  27. Bajaj R, Jaremko Å, Jaremko M, Becker S, Zweckstetter M. Molecular basis of the dynamic structure of the TIM23 complex in the mitochondrial intermembrane space. Structure. 2014;22:1501-11 pubmed publisher
    ..we characterized the dynamic interaction network of the intermembrane space domains of Tim23, Tim21, Tim50, and Tom22 at single-residue level...
  28. Melin J, Schulz C, Wrobel L, Bernhard O, Chacinska A, Jahn O, et al. Presequence recognition by the tom40 channel contributes to precursor translocation into the mitochondrial matrix. Mol Cell Biol. 2014;34:3473-85 pubmed publisher
    ..the relation between the identified presequence-binding region of Tom40 and the intermembrane space domain of Tom22. The identified presequence-interacting region of Tom40 is capable of functioning independently of the established ..
  29. Gornicka A, Bragoszewski P, Chrościcki P, Wenz L, Schulz C, Rehling P, et al. A discrete pathway for the transfer of intermembrane space proteins across the outer membrane of mitochondria. Mol Biol Cell. 2014;25:3999-4009 pubmed publisher
    ..outer membrane translocation did not directly involve other core components of the TOM complex, including Tom22. Thus MIA-dependent proteins take another route across the outer mitochondrial membrane that involves Tom40 in a ..
  30. Gerbeth C, Schmidt O, Rao S, Harbauer A, Mikropoulou D, Opalińska M, et al. Glucose-induced regulation of protein import receptor Tom22 by cytosolic and mitochondria-bound kinases. Cell Metab. 2013;18:578-87 pubmed publisher
    Most mitochondrial proteins are imported by the translocase of the outer mitochondrial membrane (TOM). Tom22 functions as central receptor and transfers preproteins to the import pore...
  31. Wenz L, Ellenrieder L, Qiu J, Bohnert M, Zufall N, van der Laan M, et al. Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis. J Cell Biol. 2015;210:1047-54 pubmed publisher
    ..Sam37 interacts with the receptor domain of Tom22 on the cytosolic side of the mitochondrial outer membrane and links TOM and SAM complexes...
  32. Reinhold R, Krüger V, Meinecke M, Schulz C, Schmidt B, Grunau S, et al. The channel-forming Sym1 protein is transported by the TIM23 complex in a presequence-independent manner. Mol Cell Biol. 2012;32:5009-21 pubmed publisher
  33. Tamura Y, Iijima M, Sesaki H. Mdm35p imports Ups proteins into the mitochondrial intermembrane space by functional complex formation. EMBO J. 2010;29:2875-87 pubmed publisher
    ..These findings provide a novel mechanism in which the formation of functional protein complexes drives mitochondrial protein import. ..
  34. Model K, Meisinger C, Kuhlbrandt W. Cryo-electron microscopy structure of a yeast mitochondrial preprotein translocase. J Mol Biol. 2008;383:1049-57 pubmed publisher
    ..Nitrilotriacetic acid-gold labeling indicates that three Tom22 subunits in the TOM complex are located at the perimeter of the complex near the interface of the globular domains...
  35. Stan T, Brix J, Schneider Mergener J, Pfanner N, Neupert W, Rapaport D. Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex. Mol Cell Biol. 2003;23:2239-50 pubmed
    ..The transmembrane domain is not required for stable binding to the TOM complex. The Tom receptors (Tom70, Tom22 and Tom20), as determined by peptide scan analysis, interact with the presequence-like helix, yet the highest ..
  36. Wittke S, Lewke N, Muller S, Johnsson N. Probing the molecular environment of membrane proteins in vivo. Mol Biol Cell. 1999;10:2519-30 pubmed
    ..The RUra3p reporter should further allow the selection of new binding partners of Sec63p and the selection of molecules or cellular conditions that interfere with the binding between Sec63p and one of its known partners. ..
  37. Hönlinger A, Bömer U, Alconada A, Eckerskorn C, Lottspeich F, Dietmeier K, et al. Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway-related role in protein import. EMBO J. 1996;15:2125-37 pubmed
    ..A lack of Tom7 stabilized the interaction between the receptors Tom20 and Tom22 and the import pore component Tom40...