TOM20

Summary

Gene Symbol: TOM20
Description: Tom20p
Alias: MAS20, MOM19, Tom20p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Popov Celeketić J, Waizenegger T, Rapaport D. Mim1 functions in an oligomeric form to facilitate the integration of Tom20 into the mitochondrial outer membrane. J Mol Biol. 2008;376:671-80 pubmed publisher
    ..Here we show that Mim1 is required for the integration of the import receptor Tom20 into the outer membrane...
  2. Dekker P, Ryan M, Brix J, Muller H, Hönlinger A, Pfanner N. Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol Cell Biol. 1998;18:6515-24 pubmed
    ..in a complex with a molecular weight of approximately 400,000 ( approximately 400K), while the other receptors, Tom20 and Tom70, are more loosely associated with this GIP complex and can be found in distinct subcomplexes...
  3. Hulett J, Lueder F, Chan N, Perry A, Wolynec P, Likic V, et al. The transmembrane segment of Tom20 is recognized by Mim1 for docking to the mitochondrial TOM complex. J Mol Biol. 2008;376:694-704 pubmed publisher
    ..The master receptor subunit of the TOM complex, Tom20, recognizes the targeting sequence on incoming mitochondrial precursor proteins, binds these protein ligands, and ..
  4. Schulz C, Rehling P. Remodelling of the active presequence translocase drives motor-dependent mitochondrial protein translocation. Nat Commun. 2014;5:4349 pubmed publisher
    ..Thus, a replenishment cycle of co-chaperones at the TIM23 complex is an integral part of Hsp70's ATPase cycle at the channel exit site and essential to maintain motor-driven mitochondrial protein import. ..
  5. Court D, Nargang F, Steiner H, Hodges R, Neupert W, Lill R. Role of the intermembrane-space domain of the preprotein receptor Tom22 in protein import into mitochondria. Mol Cell Biol. 1996;16:4035-42 pubmed
    ..The N-terminal domain of Tom22 functions as a preprotein receptor in cooperation with Tom20. The role of the C-terminal domain of Tom22, which is exposed to the intermembrane space (IMS), in its own ..
  6. Becker T, Wenz L, Krüger V, Lehmann W, Müller J, Goroncy L, et al. The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins. J Cell Biol. 2011;194:387-95 pubmed publisher
    ..We conclude that the Mim1 complex plays a central role in the import of ?-helical outer membrane proteins with multiple transmembrane segments. ..
  7. Thornton N, Stroud D, Milenkovic D, Guiard B, Pfanner N, Becker T. Two modular forms of the mitochondrial sorting and assembly machinery are involved in biogenesis of alpha-helical outer membrane proteins. J Mol Biol. 2010;396:540-9 pubmed publisher
    ..We suggest that the modular composition of the SAM complex provides a flexible platform to integrate the sorting pathways of different precursor proteins and to promote their assembly into oligomeric complexes. ..
  8. Chan N, Lithgow T. The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis. Mol Biol Cell. 2008;19:126-36 pubmed
    ..Very different environments surround bacteria and mitochondria, and we discuss the role of Sam35 and Sam37 in terms of the problems peculiar to mitochondrial protein substrates. ..
  9. Burri L, Vascotto K, Gentle I, Chan N, Beilharz T, Stapleton D, et al. Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae. FEBS J. 2006;273:1507-15 pubmed
    ..The alpha-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane. ..

More Information

Publications45

  1. Model K, Meisinger C, Prinz T, Wiedemann N, Truscott K, Pfanner N, et al. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001;8:361-70 pubmed
    ..The precursor of the channel-lining Tom40 is first targeted to the membrane via the receptor proteins Tom20 and Tom22; it then assembles with Tom5 to form the 250 kDa intermediate exposed to the intermembrane space...
  2. van Wilpe S, Ryan M, Hill K, Maarse A, Meisinger C, Brix J, et al. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature. 1999;401:485-9 pubmed
    ..between the core complexes, whereas its cytosolic domain serves as docking point for the peripheral receptors Tom20 and Tom70...
  3. Brix J, Dietmeier K, Pfanner N. Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70. J Biol Chem. 1997;272:20730-5 pubmed
    ..We have expressed and purified the cytosolic domains of three postulated import receptors, Tom20, Tom22, and Tom70. Each receptor domain is able to bind mitochondrial preproteins but with different specificity...
  4. Hönlinger A, Kubrich M, Moczko M, Gartner F, Mallet L, Bussereau F, et al. The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins. Mol Cell Biol. 1995;15:3382-9 pubmed
    ..both receptors to the general insertion pore or a more limited function dependent on the presence of the receptor Mom19. For this report, we identified and cloned Saccharomyces cerevisiae MOM22 and investigated whether it plays a ..
  5. Rapaport D, Neupert W. Biogenesis of Tom40, core component of the TOM complex of mitochondria. J Cell Biol. 1999;146:321-31 pubmed
    ..Initially, Tom40 is bound as a monomer at the mitochondrial surface. The import receptor Tom20 is involved in this initial step; it stimulates both binding and efficient insertion of the Tom40 precursor...
  6. Krimmer T, Rapaport D, Ryan M, Meisinger C, Kassenbrock C, Blachly Dyson E, et al. Biogenesis of porin of the outer mitochondrial membrane involves an import pathway via receptors and the general import pore of the TOM complex. J Cell Biol. 2001;152:289-300 pubmed
    ..The process of import and assembly of the protein is known to be dependent on the surface receptor Tom20, but the requirement for other mitochondrial proteins remains controversial...
  7. Becker T, Wenz L, Thornton N, Stroud D, Meisinger C, Wiedemann N, et al. Biogenesis of mitochondria: dual role of Tom7 in modulating assembly of the preprotein translocase of the outer membrane. J Mol Biol. 2011;405:113-24 pubmed publisher
    ..Thus, Tom7 modulates the biogenesis of topologically different proteins, the ?-barrel forming protein Tom40 and Tom22 that contains a transmembrane ?-helix. ..
  8. Young J, Hoogenraad N, Hartl F. Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70. Cell. 2003;112:41-50 pubmed
    ..We outline a novel mechanism in which chaperones are recruited for a specific targeting event by a membrane-bound receptor. ..
  9. Moczko M, Ehmann B, Gartner F, Hönlinger A, Schafer E, Pfanner N. Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria. J Biol Chem. 1994;269:9045-51 pubmed
    The mitochondrial outer membrane proteins MOM19 and MOM72 are thought to function as import receptors for nuclear encoded preproteins...
  10. Yamano K, Yatsukawa Y, Esaki M, Hobbs A, Jensen R, Endo T. Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. J Biol Chem. 2008;283:3799-807 pubmed
    Precise targeting of mitochondrial precursor proteins to mitochondria requires receptor functions of Tom20, Tom22, and Tom70 on the mitochondrial surface...
  11. Dietmeier K, Hönlinger A, Bömer U, Dekker P, Eckerskorn C, Lottspeich F, et al. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature. 1997;388:195-200 pubmed
    ..b>Tom20-Tom22 and Tom70-Tom37 function as import receptors with a preference for preproteins that have amino-terminal ..
  12. Becker T, Pfannschmidt S, Guiard B, Stojanovski D, Milenkovic D, Kutik S, et al. Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors. J Biol Chem. 2008;283:120-7 pubmed
    ..The three receptors Tom20, Tom22, and Tom70 are anchored in the outer membrane by a single transmembrane alpha-helix, located at the N ..
  13. Haucke V, Horst M, Schatz G, Lithgow T. The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor. EMBO J. 1996;15:1231-7 pubmed
    ..We conclude that the two receptor subcomplexes of the mitochondrial protein import receptor interact in vivo via their Mas20p and Mas70p subunits and that this interaction is functionally important. ..
  14. Papic D, Elbaz Alon Y, Koerdt S, Leopold K, Worm D, Jung M, et al. The role of Djp1 in import of the mitochondrial protein Mim1 demonstrates specificity between a cochaperone and its substrate protein. Mol Cell Biol. 2013;33:4083-94 pubmed publisher
    ..Taking our results together, we identified Tom70 and Djp1 as crucial players in the biogenesis of Mim1. Moreover, the involvement of Djp1 provides a unique case of specificity between a cochaperone and its substrate protein. ..
  15. Gornicka A, Bragoszewski P, Chrościcki P, Wenz L, Schulz C, Rehling P, et al. A discrete pathway for the transfer of intermembrane space proteins across the outer membrane of mitochondria. Mol Biol Cell. 2014;25:3999-4009 pubmed publisher
    ..Thus MIA-dependent proteins take another route across the outer mitochondrial membrane that involves Tom40 in a form that is different from the canonical TOM complex. ..
  16. Tamura Y, Iijima M, Sesaki H. Mdm35p imports Ups proteins into the mitochondrial intermembrane space by functional complex formation. EMBO J. 2010;29:2875-87 pubmed publisher
    ..These findings provide a novel mechanism in which the formation of functional protein complexes drives mitochondrial protein import. ..
  17. Merklinger E, Gofman Y, Kedrov A, Driessen A, Ben Tal N, Shai Y, et al. Membrane integration of a mitochondrial signal-anchored protein does not require additional proteinaceous factors. Biochem J. 2012;442:381-9 pubmed publisher
    ..Collectively, our results suggest that SA proteins are specifically inserted into the MOM by a process that is not dependent on additional proteins, but is rather facilitated by the distinct lipid composition of this membrane. ..
  18. Hoseini H, Pandey S, Jores T, Schmitt A, Franz Wachtel M, Macek B, et al. The cytosolic cochaperone Sti1 is relevant for mitochondrial biogenesis and morphology. FEBS J. 2016;283:3338-52 pubmed publisher
    ..In addition, double deletion of STI1 with the mitochondrial import factors, MIM1 or TOM20, showed a synthetic growth phenotype indicating a genetic interaction of STI1 with these genes...
  19. Eliyahu E, Pnueli L, Melamed D, Scherrer T, Gerber A, Pines O, et al. Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner. Mol Cell Biol. 2010;30:284-94 pubmed publisher
    ..Taken together, our data reveal a large-scale mRNA association mode that involves interaction of Tom20p with the translated mitochondrial targeting sequence and may be assisted by Puf3p.
  20. Wittke S, Lewke N, Muller S, Johnsson N. Probing the molecular environment of membrane proteins in vivo. Mol Biol Cell. 1999;10:2519-30 pubmed
    ..The RUra3p reporter should further allow the selection of new binding partners of Sec63p and the selection of molecules or cellular conditions that interfere with the binding between Sec63p and one of its known partners. ..
  21. Wenz L, Ellenrieder L, Qiu J, Bohnert M, Zufall N, van der Laan M, et al. Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis. J Cell Biol. 2015;210:1047-54 pubmed publisher
    ..Sam37 thus promotes efficient transfer of β-barrel precursors to the SAM complex. We conclude that Sam37 functions as a coupling factor of the translocase supercomplex of the mitochondrial outer membrane. ..
  22. Reinhold R, Krüger V, Meinecke M, Schulz C, Schmidt B, Grunau S, et al. The channel-forming Sym1 protein is transported by the TIM23 complex in a presequence-independent manner. Mol Cell Biol. 2012;32:5009-21 pubmed publisher
  23. Model K, Meisinger C, Kuhlbrandt W. Cryo-electron microscopy structure of a yeast mitochondrial preprotein translocase. J Mol Biol. 2008;383:1049-57 pubmed publisher
    ..We determined the structure of the native, unstained approximately 550-kDa core-Tom20 complex from Saccharomycescerevisiae by cryo-electron microscopy at 18-A resolution...
  24. Model K, Prinz T, Ruiz T, Radermacher M, Krimmer T, Kuhlbrandt W, et al. Protein translocase of the outer mitochondrial membrane: role of import receptors in the structural organization of the TOM complex. J Mol Biol. 2002;316:657-66 pubmed
    ..This translocase of the outer membrane (TOM) consists of three receptor proteins, Tom20, Tom22 and Tom70, the channel protein Tom40, and several small Tom proteins...
  25. Yamamoto H, Itoh N, Kawano S, Yatsukawa Y, Momose T, Makio T, et al. Dual role of the receptor Tom20 in specificity and efficiency of protein import into mitochondria. Proc Natl Acad Sci U S A. 2011;108:91-6 pubmed publisher
    Mitochondria import most of their resident proteins from the cytosol, and the import receptor Tom20 of the outer-membrane translocator TOM40 complex plays an essential role in specificity of mitochondrial protein import...
  26. Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
    ..We conclude that cytosolic kinases exert stimulatory and inhibitory effects on biogenesis and function of the TOM complex and thus regulate protein import into mitochondria. ..
  27. Tarassov I, Entelis N, Martin R. An intact protein translocating machinery is required for mitochondrial import of a yeast cytoplasmic tRNA. J Mol Biol. 1995;245:315-23 pubmed
    ..and inner mitochondrial membrane receptors shown to participate in translocation of mitochondrial pre-proteins (MOM19 and MPI1/MIM44, respectively) are also important for import of the tRNA, in vitro as well as in vivo...
  28. Moczko M, Dietmeier K, Sollner T, Segui B, Steger H, Neupert W, et al. Identification of the mitochondrial receptor complex in Saccharomyces cerevisiae. FEBS Lett. 1992;310:265-8 pubmed
    ..In Neurospora crassa, the two import receptors, MOM19 and MOM72, were found in a complex with the general insertion protein, GIP (formed by MOM7, MOM8, MOM30 and MOM38) ..
  29. Gerbeth C, Schmidt O, Rao S, Harbauer A, Mikropoulou D, Opalińska M, et al. Glucose-induced regulation of protein import receptor Tom22 by cytosolic and mitochondria-bound kinases. Cell Metab. 2013;18:578-87 pubmed publisher
    ..CK1 phosphorylates Tom22 at Thr57 and stimulates the assembly of Tom22 and Tom20. In contrast, protein kinase A (PKA), which is also activated by the addition of glucose, phosphorylates the ..
  30. Ramage L, Junne T, Hahne K, Lithgow T, Schatz G. Functional cooperation of mitochondrial protein import receptors in yeast. EMBO J. 1993;12:4115-23 pubmed
    We have identified a 20 kDa yeast mitochondrial outer membrane protein (termed MAS20) which appears to function as a protein import receptor...
  31. Hönlinger A, Bömer U, Alconada A, Eckerskorn C, Lottspeich F, Dietmeier K, et al. Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway-related role in protein import. EMBO J. 1996;15:2125-37 pubmed
    ..A lack of Tom7 stabilized the interaction between the receptors Tom20 and Tom22 and the import pore component Tom40...
  32. Shiota T, Mabuchi H, Tanaka Yamano S, Yamano K, Endo T. In vivo protein-interaction mapping of a mitochondrial translocator protein Tom22 at work. Proc Natl Acad Sci U S A. 2011;108:15179-83 pubmed publisher
    ..multifunctional subunit of the outer membrane translocator TOM40 complex, with other translocator subunits such as Tom20, Tom40, and Tim50 and with substrate precursor proteins at a spatial resolution of the amino acid residue by in ..
  33. Bohnert M, Wenz L, Zerbes R, Horvath S, Stroud D, von der Malsburg K, et al. Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane. Mol Biol Cell. 2012;23:3948-56 pubmed publisher
    ..We conclude that MINOS interacts with TOM and SAM independently and that the core subunit mitofilin is involved in biogenesis of outer membrane ?-barrel proteins. ..
  34. Gratzer S, Lithgow T, Bauer R, Lamping E, Paltauf F, Kohlwein S, et al. Mas37p, a novel receptor subunit for protein import into mitochondria. J Cell Biol. 1995;129:25-34 pubmed
  35. Motley A, Galvin P, Ekal L, Nuttall J, Hettema E. Reevaluation of the role of Pex1 and dynamin-related proteins in peroxisome membrane biogenesis. J Cell Biol. 2015;211:1041-56 pubmed publisher
    ..Our data are compatible with the established primary role of the Pex1/Pex6 complex in matrix protein import and show that peroxisomes in Saccharomyces cerevisiae multiply mainly by growth and division. ..
  36. Rucktäschel R, Halbach A, Girzalsky W, Rottensteiner H, Erdmann R. De novo synthesis of peroxisomes upon mitochondrial targeting of Pex3p. Eur J Cell Biol. 2010;89:947-54 pubmed publisher
    ..Pex3p was targeted to mitochondria by fusion with the mitochondrial targeting signal of Tom20p. The Tom20p-Pex3p-fusion protein was expressed in Pex3p-deficient cells, which are characterized by the lack of ..