Gene Symbol: TIR1
Description: GPI-anchored mannoprotein
Alias: SRP1, GPI-anchored mannoprotein
Species: Saccharomyces cerevisiae S288c
Kitagaki H, Shimoi H, Itoh K. Identification and analysis of a static culture-specific cell wall protein, Tir1p/Srp1p in Saccharomyces cerevisiae. Eur J Biochem. 1997;249:343-9 pubmed
..The amino acid sequence of this protein was identical to the sequence of Tir1p/Srp1p. TIR1/SRP1 has previously been identified as a gene induced by glucose, cold shock or anaerobiosis and was believed to be ..
De Sampaïo G, Bourdineaud J, Lauquin G. A constitutive role for GPI anchors in Saccharomyces cerevisiae: cell wall targeting. Mol Microbiol. 1999;34:247-56 pubmed
..In this condition, this human signal is able to target a protein to the cell wall. Moreover, TIR1 proved to be a multicopy suppressor of Deltagas1 mutation...
Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 2009;6:917-22 pubmed publisher
Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Seraphin B. A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol. 1999;17:1030-2 pubmed
..Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms. ..
Donzeau M, Bourdineaud J, Lauquin G. Regulation by low temperatures and anaerobiosis of a yeast gene specifying a putative GPI-anchored plasma membrane protein [corrected]. Mol Microbiol. 1996;20:449-59 pubmed
Expression of the yeast Saccharomyces cerevisiae SRP1 (Serine-rich Protein) gene is shown here to be induced both by low temperature and anaerobic growth conditions...
Yin Q, De Groot P, Dekker H, De Jong L, Klis F, de Koster C. Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages. J Biol Chem. 2005;280:20894-901 pubmed
..Therefore, it will probably be equally effective in many other fungi. ..
Snoek I, Tai S, Pronk J, Yde Steensma H, Daran J. Involvement of Snf7p and Rim101p in the transcriptional regulation of TIR1 and other anaerobically upregulated genes in Saccharomyces cerevisiae. FEMS Yeast Res. 2010;10:367-84 pubmed publisher
..Experiments with a reporter construct in which the promoter of the anaerobically upregulated TIR1 gene was fused to lacZ revealed a loss of anaerobic upregulation in an snf7Delta mutant...