Gene Symbol: TIM54
Description: Tim54p
Alias: Tim54p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Rehling P, Model K, Brandner K, Kovermann P, Sickmann A, Meyer H, et al. Protein insertion into the mitochondrial inner membrane by a twin-pore translocase. Science. 2003;299:1747-51 pubmed
    ..Thus, protein insertion was driven by the coordinated action of a twin-pore complex in two voltage-dependent steps. ..
  2. Kerscher O, Holder J, Srinivasan M, Leung R, Jensen R. The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane. J Cell Biol. 1997;139:1663-75 pubmed
    We have identified a new protein, Tim54p, located in the yeast mitochondrial inner membrane...
  3. Kerscher O, Sepuri N, Jensen R. Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane. Mol Biol Cell. 2000;11:103-16 pubmed
    ..Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex)...
  4. Gebert N, Gebert M, Oeljeklaus S, von der Malsburg K, Stroud D, Kulawiak B, et al. Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane. Mol Cell. 2011;44:811-8 pubmed publisher
    ..We conclude that the assembly of Sdh3 with different partner proteins, Sdh4 and Tim18, recruits it to two different mitochondrial membrane complexes with functions in bioenergetics and protein biogenesis, respectively. ..
  5. Koehler C, Murphy M, Bally N, Leuenberger D, Oppliger W, Dolfini L, et al. Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane. Mol Cell Biol. 2000;20:1187-93 pubmed
    ..complex contains the peripheral subunits Tim9p, Tim10p, and Tim12p and the integral membrane subunits Tim22p and Tim54p. We identify here an additional subunit, an 18-kDa integral membrane protein termed Tim18p...
  6. Murphy M, Leuenberger D, Curran S, Oppliger W, Koehler C. The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex. Mol Cell Biol. 2001;21:6132-8 pubmed
    ..Like membrane-associated Tim12p, they are members of the Tim18p-Tim22p-Tim54p membrane complex that mediates precursor insertion into the membrane...
  7. Wagner K, Gebert N, Guiard B, Brandner K, Truscott K, Wiedemann N, et al. The assembly pathway of the mitochondrial carrier translocase involves four preprotein translocases. Mol Cell Biol. 2008;28:4251-60 pubmed publisher
    ..We have analyzed the assembly pathway of the three membrane-integral subunits Tim18, Tim22, and Tim54 of the twin-pore carrier translocase...
  8. Tamura Y, Endo T, Iijima M, Sesaki H. Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. J Cell Biol. 2009;185:1029-45 pubmed publisher
    ..Furthermore, we observed synthetic growth defects in ups mutants in combination with loss of Pam17p, which controls the integrity of PAM. Our findings provide a novel molecular mechanism for the regulation of cardiolipin metabolism. ..
  9. Wrobel L, Trojanowska A, Sztolsztener M, Chacinska A. Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria. Mol Biol Cell. 2013;24:543-54 pubmed publisher
    ..We propose that Mia40 not only is responsible for disulfide bond formation, but also assists the Tim22 protein in its integration into the inner membrane of mitochondria. ..

More Information


  1. Okamoto H, Miyagawa A, Shiota T, Tamura Y, Endo T. Intramolecular disulfide bond of Tim22 protein maintains integrity of the TIM22 complex in the mitochondrial inner membrane. J Biol Chem. 2014;289:4827-38 pubmed publisher
    ..Our findings provide a new insight into the mechanism of the maintenance of the structural and functional integrity of the TIM22 complex. ..
  2. Davis A, Sepuri N, Holder J, Johnson A, Jensen R. Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria. J Cell Biol. 2000;150:1271-82 pubmed
    ..The positive charges are not required for interaction with the Tim9p-Tim10p complex, but are essential for cross-linking of Tim23p to components of the inner membrane insertion machinery, including Tim54p, Tim22p, and Tim12p.
  3. Hwang D, Claypool S, Leuenberger D, Tienson H, Koehler C. Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion. J Cell Biol. 2007;178:1161-75 pubmed
    b>Tim54p, a component of the inner membrane TIM22 complex, does not directly mediate the import of inner membrane substrates but is required for assembly/stability of the 300-kD TIM22 complex...
  4. van der Laan M, Wiedemann N, Mick D, Guiard B, Rehling P, Pfanner N. A role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondria. Curr Biol. 2006;16:2271-6 pubmed
    ..These findings suggest a new coupling mechanism in an energy-transducing membrane. ..
  5. Schuler M, Di Bartolomeo F, MÃ¥rtensson C, Daum G, Becker T. Phosphatidylcholine Affects Inner Membrane Protein Translocases of Mitochondria. J Biol Chem. 2016;291:18718-29 pubmed publisher
    ..We conclude that reduced PC levels differentially affect the TIM22 and TIM23 complexes in mitochondrial protein transport. ..