TIM23

Summary

Gene Symbol: TIM23
Description: protein transporter TIM23
Alias: MAS6, MIM23, MPI3, protein transporter TIM23
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Davis A, Sepuri N, Holder J, Johnson A, Jensen R. Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria. J Cell Biol. 2000;150:1271-82 pubmed
    ..To determine how the Tim23 protein itself is transported into mitochondria, we used chemical cross-linking to identify proteins adjacent to ..
  2. Chan N, Lithgow T. The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis. Mol Biol Cell. 2008;19:126-36 pubmed
    ..Very different environments surround bacteria and mitochondria, and we discuss the role of Sam35 and Sam37 in terms of the problems peculiar to mitochondrial protein substrates. ..
  3. Kerscher O, Sepuri N, Jensen R. Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane. Mol Biol Cell. 2000;11:103-16 pubmed
    ..We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane. ..
  4. Stojanovski D, Milenkovic D, Müller J, Gabriel K, Schulze Specking A, Baker M, et al. Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase. J Cell Biol. 2008;183:195-202 pubmed publisher
  5. Kerscher O, Holder J, Srinivasan M, Leung R, Jensen R. The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane. J Cell Biol. 1997;139:1663-75 pubmed
    ..First, multiple copies of the TIM22 gene, but not TIM23 or TIM17, suppress the growth defect of a tim54-1 temperature-sensitive mutant...
  6. Mokranjac D, Sichting M, Popov Celeketic D, Mapa K, Gevorkyan Airapetov L, Zohary K, et al. Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria. Mol Biol Cell. 2009;20:1400-7 pubmed publisher
    ..and requires a coordinated action of the translocases of outer and inner mitochondrial membranes (TOM and TIM23 complexes)...
  7. Dienhart M, Stuart R. The yeast Aac2 protein exists in physical association with the cytochrome bc1-COX supercomplex and the TIM23 machinery. Mol Biol Cell. 2008;19:3934-43 pubmed publisher
    ..with the cytochrome c reductase (cytochrome bc(1))-cytochrome c oxidase (COX) supercomplex and its associated TIM23 machinery...
  8. Leuenberger D, Bally N, Schatz G, Koehler C. Different import pathways through the mitochondrial intermembrane space for inner membrane proteins. EMBO J. 1999;18:4816-22 pubmed
    ..that they can transfer these proteins to one of three different membrane insertion sites: the TIM22 complex, the TIM23 complex or an as yet uncharacterized insertion site...
  9. Alder N, Jensen R, Johnson A. Fluorescence mapping of mitochondrial TIM23 complex reveals a water-facing, substrate-interacting helix surface. Cell. 2008;134:439-50 pubmed publisher
    Protein translocation across the mitochondrial inner membrane is mediated by the TIM23 complex...

More Information

Publications72

  1. Geissler A, Chacinska A, Truscott K, Wiedemann N, Brandner K, Sickmann A, et al. The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell. 2002;111:507-18 pubmed
    ..Tim50 interacts with preproteins in transit and directs them to the channel protein Tim23. Inactivation of Tim50 strongly inhibits the import of preproteins with a classical matrix-targeting signal, while ..
  2. Chacinska A, van der Laan M, Mehnert C, Guiard B, Mick D, Hutu D, et al. Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting. Mol Cell Biol. 2010;30:307-18 pubmed publisher
    ..the translocase of the outer membrane (TOM) associates with the presequence translocase of the inner membrane (TIM23) in a supercomplex...
  3. Hutu D, Guiard B, Chacinska A, Becker D, Pfanner N, Rehling P, et al. Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocase. Mol Biol Cell. 2008;19:2642-9 pubmed publisher
    The presequence translocase of the mitochondrial inner membrane (TIM23 complex) mediates the import of preproteins with amino-terminal presequences...
  4. Bömer U, Meijer M, Maarse A, Hönlinger A, Dekker P, Pfanner N, et al. Multiple interactions of components mediating preprotein translocation across the inner mitochondrial membrane. EMBO J. 1997;16:2205-16 pubmed
    ..The protein transport machinery of the inner mitochondrial membrane contains three essential Tim proteins. Tim17 and Tim23 are thought to build a preprotein translocation channel, while Tim44 transiently interacts with the matrix heat ..
  5. Berthold J, Bauer M, Schneider H, Klaus C, Dietmeier K, Neupert W, et al. The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell. 1995;81:1085-93 pubmed
    ..Two known components, MIM23 and MIM17, and two novel components, MIM33 and MIM14, were found as constituents of this complex...
  6. D Silva P, Schilke B, Hayashi M, Craig E. Interaction of the J-protein heterodimer Pam18/Pam16 of the mitochondrial import motor with the translocon of the inner membrane. Mol Biol Cell. 2008;19:424-32 pubmed
    Import of proteins across the inner mitochondrial membrane through the Tim23:Tim17 translocase requires the function of an essential import motor having mitochondrial 70-kDa heat-shock protein (mtHsp70) at its core...
  7. Popov Celeketic D, Mapa K, Neupert W, Mokranjac D. Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria. EMBO J. 2008;27:1469-80 pubmed publisher
    The TIM23 (translocase of the mitochondrial inner membrane) complex mediates translocation of preproteins across and their insertion into the mitochondrial inner membrane...
  8. van der Laan M, Wiedemann N, Mick D, Guiard B, Rehling P, Pfanner N. A role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondria. Curr Biol. 2006;16:2271-6 pubmed
    ..These findings suggest a new coupling mechanism in an energy-transducing membrane. ..
  9. Sanjuán Szklarz L, Guiard B, Rissler M, Wiedemann N, Kozjak V, van der Laan M, et al. Inactivation of the mitochondrial heat shock protein zim17 leads to aggregation of matrix hsp70s followed by pleiotropic effects on morphology and protein biogenesis. J Mol Biol. 2005;351:206-18 pubmed
  10. Dekker P, Martin F, Maarse A, Bömer U, Muller H, Guiard B, et al. The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44. EMBO J. 1997;16:5408-19 pubmed
    ..By blue native electrophoresis, we identify an approximately 90K complex with assembled Tim23 and Tim17 as the core of the inner membrane import site for presequence-containing preproteins...
  11. Popov Celeketic D, Waegemann K, Mapa K, Neupert W, Mokranjac D. Role of the import motor in insertion of transmembrane segments by the mitochondrial TIM23 complex. EMBO Rep. 2011;12:542-8 pubmed publisher
    The TIM23 complex mediates translocation of proteins across, and their lateral insertion into, the mitochondrial inner membrane...
  12. Schiller D, Cheng Y, Liu Q, Walter W, Craig E. Residues of Tim44 involved in both association with the translocon of the inner mitochondrial membrane and regulation of mitochondrial Hsp70 tethering. Mol Cell Biol. 2008;28:4424-33 pubmed publisher
  13. Donzeau M, Kaldi K, Adam A, Paschen S, Wanner G, Guiard B, et al. Tim23 links the inner and outer mitochondrial membranes. Cell. 2000;101:401-12 pubmed
    b>Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor...
  14. Sirrenberg C, Endres M, FOLSCH H, Stuart R, Neupert W, Brunner M. Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5. Nature. 1998;391:912-5 pubmed
    ..carrying amino-terminal targeting signals are translocated into the matrix by the integral inner membrane proteins Tim23 and Tim17 in cooperation with Tim44 and mitochondrial Hsp70...
  15. Mokranjac D, Sichting M, Neupert W, Hell K. Tim14, a novel key component of the import motor of the TIM23 protein translocase of mitochondria. EMBO J. 2003;22:4945-56 pubmed
    The TIM23 translocase mediates the deltaPsi- and ATP-dependent import of proteins into mitochondria. We identified Tim14 as a novel component of the TIM23 translocase...
  16. de la Cruz L, Bajaj R, Becker S, Zweckstetter M. The intermembrane space domain of Tim23 is intrinsically disordered with a distinct binding region for presequences. Protein Sci. 2010;19:2045-54 pubmed publisher
    ..protein complexes, the translocase of the outer membrane (TOM) and one of the translocases of the inner membrane (TIM23)...
  17. van der Laan M, Meinecke M, Dudek J, Hutu D, Lind M, Perschil I, et al. Motor-free mitochondrial presequence translocase drives membrane integration of preproteins. Nat Cell Biol. 2007;9:1152-9 pubmed
    ..Thus, the minimal system for preprotein integration into the mitochondrial inner membrane is the presequence translocase, a cardiolipin-rich membrane and a membrane potential. ..
  18. Blom J, Dekker P, Meijer M. Functional and physical interactions of components of the yeast mitochondrial inner-membrane import machinery (MIM). Eur J Biochem. 1995;232:309-14 pubmed
    ..Two other putative components of this protein-translocation system are the integral inner-membrane proteins, Mim23 and Mim17. Here, we present genetic evidence for functional co-operation of all three proteins...
  19. Alder N, Sutherland J, Buhring A, Jensen R, Johnson A. Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits. Mol Biol Cell. 2008;19:159-70 pubmed
    Tim23p is an essential channel-forming component of the multisubunit TIM23 complex of the mitochondrial inner membrane that mediates protein import...
  20. Qian X, Gebert M, Höpker J, Yan M, Li J, Wiedemann N, et al. Structural basis for the function of Tim50 in the mitochondrial presequence translocase. J Mol Biol. 2011;411:513-9 pubmed publisher
    ..Tim50 and Tim23 transfer preproteins through the intermembrane space to the inner membrane...
  21. Mokranjac D, Berg A, Adam A, Neupert W, Hell K. Association of the Tim14.Tim16 subcomplex with the TIM23 translocase is crucial for function of the mitochondrial protein import motor. J Biol Chem. 2007;282:18037-45 pubmed
    Tim14 and Tim16 are essential components of the import motor of the mitochondrial TIM23 preprotein translocase. Tim14 contains a J domain in the matrix space that is anchored in the inner membrane by a transmembrane segment...
  22. Ryan K, Menold M, Garrett S, Jensen R. SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import. Mol Biol Cell. 1994;5:529-38 pubmed
    b>MAS6 encodes an essential inner membrane protein required for mitochondrial protein import in the yeast Saccharomyces cerevisiae (Emtage and Jensen, 1993)...
  23. Chacinska A, Lind M, Frazier A, Dudek J, Meisinger C, Geissler A, et al. Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell. 2005;120:817-29 pubmed
    The presequence translocase of the inner mitochondrial membrane (TIM23 complex) operates at a central junction of protein import...
  24. Kozany C, Mokranjac D, Sichting M, Neupert W, Hell K. The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase. Nat Struct Mol Biol. 2004;11:234-41 pubmed
    Mitochondria import the vast majority of their proteins from the cytosol. The mitochondrial import motor of the TIM23 translocase drives the translocation of precursor proteins across the outer and inner membrane in an ATP-dependent ..
  25. Wong E, Wagner J, Gorsich S, McCaffery J, Shaw J, Nunnari J. The dynamin-related GTPase, Mgm1p, is an intermembrane space protein required for maintenance of fusion competent mitochondria. J Cell Biol. 2000;151:341-52 pubmed
  26. Truscott K, Kovermann P, Geissler A, Merlin A, Meijer M, Driessen A, et al. A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23. Nat Struct Biol. 2001;8:1074-82 pubmed
    ..Here we report that purified Tim23 forms a hydrophilic, approximately 13-24 A wide channel characteristic of the mitochondrial presequence ..
  27. van der Laan M, Chacinska A, Lind M, Perschil I, Sickmann A, Meyer H, et al. Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol Cell Biol. 2005;25:7449-58 pubmed
    ..Our findings suggest that Pam17 is required for the correct organization of the Pam16-Pam18 complex and thus contributes to regulation of mtHsp70 activity at the inner membrane translocation site. ..
  28. Wiedemann N, van der Laan M, Hutu D, Rehling P, Pfanner N. Sorting switch of mitochondrial presequence translocase involves coupling of motor module to respiratory chain. J Cell Biol. 2007;179:1115-22 pubmed
    ..The motor does not assemble en bloc with the translocase but apparently in a step-wise manner with the Pam16/18 module before the Hsp70 core. ..
  29. Tamura Y, Harada Y, Yamano K, Watanabe K, Ishikawa D, Ohshima C, et al. Identification of Tam41 maintaining integrity of the TIM23 protein translocator complex in mitochondria. J Cell Biol. 2006;174:631-7 pubmed
    ..TAM41 gene led to temperature-sensitive growth of yeast cells and resulted in defects in protein import via the TIM23 translocator complex at elevated temperature both in vivo and in vitro...
  30. Martinez Caballero S, Grigoriev S, Herrmann J, Campo M, Kinnally K. Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23. J Biol Chem. 2007;282:3584-93 pubmed
    The TIM23 complex mediates import of preproteins into mitochondria, but little is known of the mechanistic properties of this translocase...
  31. Mokranjac D, Popov Celeketic D, Hell K, Neupert W. Role of Tim21 in mitochondrial translocation contact sites. J Biol Chem. 2005;280:23437-40 pubmed
    ..of the translocase of the outer membrane (TOM complex) and the presequence translocase of the inner membrane (TIM23 complex). However, the molecular nature of the translocation contact sites is poorly understood...
  32. Chacinska A, Rehling P, Guiard B, Frazier A, Schulze Specking A, Pfanner N, et al. Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J. 2003;22:5370-81 pubmed
    ..include the translocase of the outer membrane (TOM complex) and the presequence translocase of the inner membrane (TIM23 complex). Little is known about the functional cooperation of these translocases...
  33. Bauer M, Sirrenberg C, Neupert W, Brunner M. Role of Tim23 as voltage sensor and presequence receptor in protein import into mitochondria. Cell. 1996;87:33-41 pubmed
    b>Tim23, an essential component of the protein import machinery of the inner membrane of mitochondria (TIM complex), forms dimers that display a dynamic behavior. Dimer formation is promoted by the membrane potential delta psi...
  34. Ryan K, Leung R, Jensen R. Characterization of the mitochondrial inner membrane translocase complex: the Tim23p hydrophobic domain interacts with Tim17p but not with other Tim23p molecules. Mol Cell Biol. 1998;18:178-87 pubmed
    ..We found that overexpression of Tim23Cp is toxic to yeast cells that carry the tim23-1 mutation...
  35. Marom M, Dayan D, Demishtein Zohary K, Mokranjac D, Neupert W, Azem A. Direct interaction of mitochondrial targeting presequences with purified components of the TIM23 protein complex. J Biol Chem. 2011;286:43809-15 pubmed publisher
    ..positively charged amphipathic presequences and cross the inner membrane with the help of vital components of the TIM23 complex. It is currently unclear which subunits of the TIM23 complex recognize and directly bind to presequences...
  36. Yamamoto H, Esaki M, Kanamori T, Tamura Y, Nishikawa S, Endo T. Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes. Cell. 2002;111:519-28 pubmed
    ..site-specific photocrosslinking of translocation intermediates, we have identified Tim50, a component of the yeast TIM23 import machinery, which mediates translocation of presequence-containing proteins across the mitochondrial inner ..
  37. Frazier A, Dudek J, Guiard B, Voos W, Li Y, Lind M, et al. Pam16 has an essential role in the mitochondrial protein import motor. Nat Struct Mol Biol. 2004;11:226-33 pubmed
    ..Thus, Pam16 is a newly identified type of motor subunit and is required to promote a functional PAM reaction cycle, thereby driving preprotein import into the matrix. ..
  38. Saddar S, Dienhart M, Stuart R. The F1F0-ATP synthase complex influences the assembly state of the cytochrome bc1-cytochrome oxidase supercomplex and its association with the TIM23 machinery. J Biol Chem. 2008;283:6677-86 pubmed publisher
    ..In addition, an increase in the level of the TIM23 translocase associated with the cytochrome bc1-COX supercomplex is observed in the absence of Su e and Su g...
  39. Gevorkyan Airapetov L, Zohary K, Popov Celeketic D, Mapa K, Hell K, Neupert W, et al. Interaction of Tim23 with Tim50 Is essential for protein translocation by the mitochondrial TIM23 complex. J Biol Chem. 2009;284:4865-72 pubmed publisher
    The TIM23 complex is the major translocase of the mitochondrial inner membrane responsible for the import of essentially all matrix proteins and a number of inner membrane proteins...
  40. SCHULKE N, Sepuri N, Gordon D, Saxena S, Dancis A, Pain D. A multisubunit complex of outer and inner mitochondrial membrane protein translocases stabilized in vivo by translocation intermediates. J Biol Chem. 1999;274:22847-54 pubmed
    ..Tom and Tim components known to be essential for import of matrix-targeted proteins, namely Tom40, Tom22, Tim17, Tim23, Tim44, and matrix-localized Hsp70...
  41. Schilke B, Hayashi M, Craig E. Genetic analysis of complex interactions among components of the mitochondrial import motor and translocon in Saccharomyces cerevisiae. Genetics. 2012;190:1341-53 pubmed publisher
  42. Malhotra K, Sathappa M, Landin J, Johnson A, Alder N. Structural changes in the mitochondrial Tim23 channel are coupled to the proton-motive force. Nat Struct Mol Biol. 2013;20:965-72 pubmed publisher
    b>Tim23, the central subunit of the TIM23 protein-translocation complex, forms a voltage-gated channel in the mitochondrial inner membrane (MIM), an energy-conserving membrane that generates a proton-motive force to drive vital processes...
  43. Mehnert C, Rampelt H, Gebert M, Oeljeklaus S, Schrempp S, Kochbeck L, et al. The mitochondrial ADP/ATP carrier associates with the inner membrane presequence translocase in a stoichiometric manner. J Biol Chem. 2014;289:27352-62 pubmed publisher
    ..The presequence translocase of the inner membrane (TIM23 complex) mediates the import of these preproteins...
  44. Banerjee R, Gladkova C, Mapa K, Witte G, Mokranjac D. Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein. elife. 2015;4:e11897 pubmed publisher
    The majority of mitochondrial proteins are targeted to mitochondria by N-terminal presequences and use the TIM23 complex for their translocation across the mitochondrial inner membrane...
  45. Stuart R. Chapter 11 Supercomplex organization of the yeast respiratory chain complexes and the ADP/ATP carrier proteins. Methods Enzymol. 2009;456:191-208 pubmed publisher
    ..g., the TIM23 machinery and also the Shy1/Cox14 proteins)...
  46. Reif S, Randelj O, Domanska G, Dian E, Krimmer T, Motz C, et al. Conserved mechanism of Oxa1 insertion into the mitochondrial inner membrane. J Mol Biol. 2005;354:520-8 pubmed
    ..We found that immediately after import into mitochondria, Oxa1 initially accumulates at Tim23 that forms the inner membrane protein translocase...
  47. Sichting M, Mokranjac D, Azem A, Neupert W, Hell K. Maintenance of structure and function of mitochondrial Hsp70 chaperones requires the chaperone Hep1. EMBO J. 2005;24:1046-56 pubmed
    ..This process is efficiently counteracted by Hep1. We conclude that Hep1 acts as a chaperone that is necessary and sufficient to prevent self-aggregation and to thereby maintain the function of the mitochondrial Hsp70 chaperones. ..
  48. Aiyar R, Bohnert M, Duvezin Caubet S, Voisset C, Gagneur J, Fritsch E, et al. Mitochondrial protein sorting as a therapeutic target for ATP synthase disorders. Nat Commun. 2014;5:5585 pubmed publisher
    ..Here we demonstrate that modulating the sorting of nuclear-encoded proteins into mitochondria, mediated by the TIM23 complex, proves therapeutic in both yeast and patient-derived cells exhibiting ATP synthase deficiency...
  49. Vergnolle M, Sawney H, Junne T, Dolfini L, Tokatlidis K. A cryptic matrix targeting signal of the yeast ADP/ATP carrier normally inserted by the TIM22 complex is recognized by the TIM23 machinery. Biochem J. 2005;385:173-80 pubmed
    ..This event is coordinated by the TIM23 translocase and displays all the features of the matrix-targeting pathway...
  50. Ting S, Yan N, Schilke B, Craig E. Dual interaction of scaffold protein Tim44 of mitochondrial import motor with channel-forming translocase subunit Tim23. elife. 2017;6: pubmed publisher
    ..cerevisiae, we found that both domains of Tim44 interact with the major matrix-exposed loop of Tim23, with the C-terminal domain (CTD) binding Tim17 as well...
  51. Böttinger L, Gornicka A, Czerwik T, Bragoszewski P, Loniewska Lwowska A, Schulze Specking A, et al. In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins. Mol Biol Cell. 2012;23:3957-69 pubmed publisher
    ..Thus Mia40 in cooperation with Erv1 promotes the formation of two disulfide bonds in the substrate protein, ensuring the efficiency of oxidative folding in the intermembrane space of mitochondria. ..
  52. Lytovchenko O, Melin J, Schulz C, Kilisch M, Hutu D, Rehling P. Signal recognition initiates reorganization of the presequence translocase during protein import. EMBO J. 2013;32:886-98 pubmed publisher
    ..receptor Tim50 interacts with Tim21 in a signal-sensitive manner in a process that involves the IMS-domain of the Tim23 channel...
  53. Demishtein Zohary K, Gunsel U, Marom M, Banerjee R, Neupert W, Azem A, et al. Role of Tim17 in coupling the import motor to the translocation channel of the mitochondrial presequence translocase. elife. 2017;6: pubmed publisher
    ..Mutations in TM1 and TM2 impaired the interaction of Tim17 with Tim23, component of the translocation channel, whereas mutations in TM3 compromised binding of the import motor...
  54. Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
    ..We conclude that cytosolic kinases exert stimulatory and inhibitory effects on biogenesis and function of the TOM complex and thus regulate protein import into mitochondria. ..
  55. Reinhold R, Krüger V, Meinecke M, Schulz C, Schmidt B, Grunau S, et al. The channel-forming Sym1 protein is transported by the TIM23 complex in a presequence-independent manner. Mol Cell Biol. 2012;32:5009-21 pubmed publisher
    ..have revealed a novel transport mechanism for a polytopic membrane protein in which internal signals direct the precursor into the inner membrane via the TIM23 complex, indicating a presequence-independent function of this translocase.
  56. Moro F, Sirrenberg C, Schneider H, Neupert W, Brunner M. The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix. EMBO J. 1999;18:3667-75 pubmed
    ..The components Tim17, Tim23 and Tim44 are present in this complex in equimolar amounts...
  57. Haucke V, Schatz G. Reconstitution of the protein insertion machinery of the mitochondrial inner membrane. EMBO J. 1997;16:4560-7 pubmed
    ..The protein insertion machinery can thus operate independently of the energy-transducing Tim44p-mhsp70 complex. ..
  58. Botelho S, Osterberg M, Reichert A, Yamano K, Björkholm P, Endo T, et al. TIM23-mediated insertion of transmembrane ?-helices into the mitochondrial inner membrane. EMBO J. 2011;30:1003-11 pubmed publisher
    ..Here, we provide comparable data for TIM23-mediated membrane protein insertion into the inner mitochondrial membrane of yeast cells...
  59. Gebert M, Schrempp S, Mehnert C, Heißwolf A, Oeljeklaus S, Ieva R, et al. Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J Cell Biol. 2012;197:595-604 pubmed publisher
    ..The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor...
  60. Curran S, Leuenberger D, Schmidt E, Koehler C. The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins. J Cell Biol. 2002;158:1017-27 pubmed
    ..Thus, polytopic inner membrane proteins, which lack an NH2-terminal targeting sequence, pass through the TOM complex as a loop followed by binding of the small Tim proteins to the hydrophobic membrane spanning domains. ..
  61. Demishtein Zohary K, Marom M, Neupert W, Mokranjac D, Azem A. GxxxG motifs hold the TIM23 complex together. FEBS J. 2015;282:2178-86 pubmed publisher
    ..The translocase of the inner mitochondrial membrane 23 (TIM23) complex is the major protein translocase of the inner membrane, and is responsible for translocation of proteins ..
  62. Bömer U, Meijer M, Guiard B, Dietmeier K, Pfanner N, Rassow J. The sorting route of cytochrome b2 branches from the general mitochondrial import pathway at the preprotein translocase of the inner membrane. J Biol Chem. 1997;272:30439-46 pubmed
    ..By co-immunoprecipitations we find that translocation intermediates of cytochrome b2 are associated with Tim23, a component of the inner membrane protein import machinery...
  63. Turakhiya U, von der Malsburg K, Gold V, Guiard B, Chacinska A, van der Laan M, et al. Protein Import by the Mitochondrial Presequence Translocase in the Absence of a Membrane Potential. J Mol Biol. 2016;428:1041-1052 pubmed publisher
    ..Precursors with a cleavable presequence are imported by the presequence translocase (TIM23 complex), while other precursors containing internal targeting signals are imported by the carrier translocase (..