Gene Symbol: TIM22
Description: translocation channel protein TIM22
Alias: translocation channel protein TIM22
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Koehler C, Jarosch E, Tokatlidis K, Schmid K, Schweyen R, Schatz G. Import of mitochondrial carriers mediated by essential proteins of the intermembrane space. Science. 1998;279:369-73 pubmed
    ..Neither protein was required for protein import into the other mitochondrial compartments. Both proteins may function as intermembrane space chaperones for the highly insoluble carrier proteins. ..
  2. Gebert N, Gebert M, Oeljeklaus S, von der Malsburg K, Stroud D, Kulawiak B, et al. Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane. Mol Cell. 2011;44:811-8 pubmed publisher
    ..We have identified a further subunit of the carrier translocase (TIM22 complex) that surprisingly is identical to subunit 3 of respiratory complex II, succinate dehydrogenase (Sdh3)...
  3. Sirrenberg C, Endres M, FOLSCH H, Stuart R, Neupert W, Brunner M. Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5. Nature. 1998;391:912-5 pubmed
    ..Tim10 and Tim12 are found in a complex with Tim22, which takes over the precursor and mediates its membrane-potential-dependent insertion into the inner membrane...
  4. Gebert N, Chacinska A, Wagner K, Guiard B, Koehler C, Rehling P, et al. Assembly of the three small Tim proteins precedes docking to the mitochondrial carrier translocase. EMBO Rep. 2008;9:548-54 pubmed publisher
    ..precursor proteins through the aqueous intermembrane space to the carrier translocase of the inner membrane (TIM22 complex)...
  5. Sirrenberg C, Bauer M, Guiard B, Neupert W, Brunner M. Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22. Nature. 1996;384:582-5 pubmed
    ..b>Tim22 is a new component of the import machinery of mitochondria, which shares sequence similarity with both Tim23 and ..
  6. Kerscher O, Sepuri N, Jensen R. Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane. Mol Biol Cell. 2000;11:103-16 pubmed
    ..We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane. ..
  7. Wagner K, Gebert N, Guiard B, Brandner K, Truscott K, Wiedemann N, et al. The assembly pathway of the mitochondrial carrier translocase involves four preprotein translocases. Mol Cell Biol. 2008;28:4251-60 pubmed publisher
    ..We have analyzed the assembly pathway of the three membrane-integral subunits Tim18, Tim22, and Tim54 of the twin-pore carrier translocase...
  8. Murphy M, Leuenberger D, Curran S, Oppliger W, Koehler C. The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex. Mol Cell Biol. 2001;21:6132-8 pubmed
    The TIM22 protein import pathway of the yeast mitochondrion contains several components, including a family of five proteins (Tim8p, -9p, -10p, -12p, and -13p [Tim, for translocase of inner membrane]) that are located in the ..
  9. Kovermann P, Truscott K, Guiard B, Rehling P, Sepuri N, Muller H, et al. Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel. Mol Cell. 2002;9:363-73 pubmed
    ..We report the unexpected observation that Tim22 is the only essential membrane-integrated subunit of the complex...

More Information


  1. Kerscher O, Holder J, Srinivasan M, Leung R, Jensen R. The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane. J Cell Biol. 1997;139:1663-75 pubmed
    ..First, multiple copies of the TIM22 gene, but not TIM23 or TIM17, suppress the growth defect of a tim54-1 temperature-sensitive mutant...
  2. Koehler C, Murphy M, Bally N, Leuenberger D, Oppliger W, Dolfini L, et al. Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane. Mol Cell Biol. 2000;20:1187-93 pubmed
    ..two soluble 70-kDa protein complexes in the intermembrane space and a 300-kDa complex in the inner membrane, the TIM22 complex...
  3. Kawano S, Yamano K, Naoé M, Momose T, Terao K, Nishikawa S, et al. Structural basis of yeast Tim40/Mia40 as an oxidative translocator in the mitochondrial intermembrane space. Proc Natl Acad Sci U S A. 2009;106:14403-7 pubmed publisher
    ..These results are consistent with the growth phenotypes of Mia40 mutant cells containing Ser replacement of the conserved cysteine residues. ..
  4. Hwang D, Claypool S, Leuenberger D, Tienson H, Koehler C. Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion. J Cell Biol. 2007;178:1161-75 pubmed
    Tim54p, a component of the inner membrane TIM22 complex, does not directly mediate the import of inner membrane substrates but is required for assembly/stability of the 300-kD TIM22 complex...
  5. Yamano K, Ishikawa D, Esaki M, Endo T. The phosphate carrier has an ability to be sorted to either the TIM22 pathway or the TIM23 pathway for its import into yeast mitochondria. J Biol Chem. 2005;280:10011-7 pubmed
    ..segments (TM1-TM6) and requires, after translocation across the outer membrane, the Tim9-Tim10 complex and the TIM22 complex to be inserted into the inner membrane...
  6. Okamoto H, Miyagawa A, Shiota T, Tamura Y, Endo T. Intramolecular disulfide bond of Tim22 protein maintains integrity of the TIM22 complex in the mitochondrial inner membrane. J Biol Chem. 2014;289:4827-38 pubmed publisher
    ..Among them, the TIM22 complex mediates insertion of polytopic membrane proteins into the inner membrane, and Tim22 constitutes its ..
  7. Peixoto P, Graña F, Roy T, Dunn C, Flores M, Jensen R, et al. Awaking TIM22, a dynamic ligand-gated channel for protein insertion in the mitochondrial inner membrane. J Biol Chem. 2007;282:18694-701 pubmed
    ..of a channel associated to the translocase of the inner membrane for the insertion of multitopic protein (TIM22) complex has been arguable, as its function relates to the insertion of multispanning proteins into the inner ..
  8. Davis A, Sepuri N, Holder J, Johnson A, Jensen R. Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria. J Cell Biol. 2000;150:1271-82 pubmed
    ..The positive charges are not required for interaction with the Tim9p-Tim10p complex, but are essential for cross-linking of Tim23p to components of the inner membrane insertion machinery, including Tim54p, Tim22p, and Tim12p. ..
  9. Brandner K, Rehling P, Truscott K. The carboxyl-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocase. J Biol Chem. 2005;280:6215-21 pubmed
    ..We concluded that, in this case, a single structural repeat can drive inner membrane insertion, whereas all three related units contribute targeting information for outer membrane translocation. ..
  10. Curran S, Leuenberger D, Leverich E, Hwang D, Beverly K, Koehler C. The role of Hot13p and redox chemistry in the mitochondrial TIM22 import pathway. J Biol Chem. 2004;279:43744-51 pubmed
    The small Tim proteins in the mitochondrial intermembrane space participate in the TIM22 import pathway for assembly of the inner membrane...
  11. Wrobel L, Trojanowska A, Sztolsztener M, Chacinska A. Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria. Mol Biol Cell. 2013;24:543-54 pubmed publisher
    ..We identify Tim22, a multispanning membrane protein and core component of the TIM22 translocase of inner membrane, as a protein with ..
  12. Schuler M, Di Bartolomeo F, Mårtensson C, Daum G, Becker T. Phosphatidylcholine Affects Inner Membrane Protein Translocases of Mitochondria. J Biol Chem. 2016;291:18718-29 pubmed publisher
    ..The carrier translocase (TIM22 complex) inserts multispanning proteins into the inner membrane...
  13. Tamura Y, Endo T, Iijima M, Sesaki H. Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. J Cell Biol. 2009;185:1029-45 pubmed publisher
    ..Furthermore, we observed synthetic growth defects in ups mutants in combination with loss of Pam17p, which controls the integrity of PAM. Our findings provide a novel molecular mechanism for the regulation of cardiolipin metabolism. ..
  14. Albrecht R, Rehling P, Chacinska A, Brix J, Cadamuro S, Volkmer R, et al. The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes. EMBO Rep. 2006;7:1233-8 pubmed
    ..We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction. ..
  15. van der Laan M, Wiedemann N, Mick D, Guiard B, Rehling P, Pfanner N. A role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondria. Curr Biol. 2006;16:2271-6 pubmed
    ..These findings suggest a new coupling mechanism in an energy-transducing membrane. ..