Gene Symbol: TIM18
Description: Tim18p
Alias: Tim18p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kerscher O, Sepuri N, Jensen R. Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane. Mol Biol Cell. 2000;11:103-16 pubmed
    ..is synthetically lethal with tim54-1; Tim54p and Tim22p can be coimmune precipitated with the Tim18 protein; and Tim18p, along with Tim54p and Tim22p, is detected in an approximately 300-kDa complex after blue native electrophoresis...
  2. Dunn C, Jensen R. Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA. Genetics. 2003;165:35-45 pubmed
    ..for the insertion of imported polytopic proteins into the mitochondrial inner membrane, contains the nonessential Tim18p subunit...
  3. Koehler C, Murphy M, Bally N, Leuenberger D, Oppliger W, Dolfini L, et al. Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane. Mol Cell Biol. 2000;20:1187-93 pubmed
    ..We identify here an additional subunit, an 18-kDa integral membrane protein termed Tim18p. This protein is made as a 21.9-kDa precursor which is imported into mitochondria and processed to its mature form...
  4. Rehling P, Model K, Brandner K, Kovermann P, Sickmann A, Meyer H, et al. Protein insertion into the mitochondrial inner membrane by a twin-pore translocase. Science. 2003;299:1747-51 pubmed
    ..Thus, protein insertion was driven by the coordinated action of a twin-pore complex in two voltage-dependent steps. ..
  5. Gebert N, Chacinska A, Wagner K, Guiard B, Koehler C, Rehling P, et al. Assembly of the three small Tim proteins precedes docking to the mitochondrial carrier translocase. EMBO Rep. 2008;9:548-54 pubmed publisher
  6. Gebert N, Gebert M, Oeljeklaus S, von der Malsburg K, Stroud D, Kulawiak B, et al. Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane. Mol Cell. 2011;44:811-8 pubmed publisher
    ..Sdh3 forms a subcomplex with Tim18 and is involved in biogenesis and assembly of the membrane-integral subunits of the TIM22 complex...
  7. Wagner K, Gebert N, Guiard B, Brandner K, Truscott K, Wiedemann N, et al. The assembly pathway of the mitochondrial carrier translocase involves four preprotein translocases. Mol Cell Biol. 2008;28:4251-60 pubmed publisher
    ..We have analyzed the assembly pathway of the three membrane-integral subunits Tim18, Tim22, and Tim54 of the twin-pore carrier translocase...
  8. Brandner K, Rehling P, Truscott K. The carboxyl-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocase. J Biol Chem. 2005;280:6215-21 pubmed
    ..We concluded that, in this case, a single structural repeat can drive inner membrane insertion, whereas all three related units contribute targeting information for outer membrane translocation. ..
  9. van der Laan M, Wiedemann N, Mick D, Guiard B, Rehling P, Pfanner N. A role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondria. Curr Biol. 2006;16:2271-6 pubmed
    ..These findings suggest a new coupling mechanism in an energy-transducing membrane. ..

More Information


  1. Mick D, Wagner K, van der Laan M, Frazier A, Perschil I, Pawlas M, et al. Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly. EMBO J. 2007;26:4347-58 pubmed
    ..We suggest that Shy1 links Cox1 translational regulation to complex IV assembly and supercomplex formation. ..
  2. Hwang D, Claypool S, Leuenberger D, Tienson H, Koehler C. Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion. J Cell Biol. 2007;178:1161-75 pubmed
    ..As such, Tim54p links protein import, assembly, and turnover pathways in the mitochondrion. ..
  3. Wrobel L, Trojanowska A, Sztolsztener M, Chacinska A. Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria. Mol Biol Cell. 2013;24:543-54 pubmed publisher
    ..We propose that Mia40 not only is responsible for disulfide bond formation, but also assists the Tim22 protein in its integration into the inner membrane of mitochondria. ..
  4. Du L, Yu Y, Li Z, Chen J, Liu Y, Xia Y, et al. Tim18, a component of the mitochondrial translocator, mediates yeast cell death induced by arsenic. Biochemistry (Mosc). 2007;72:843-7 pubmed
    Evidence is presented that Tim18, a mitochondria translocase, plays a role in the previously described apoptosis induced by arsenite in Saccharomyces cerevisiae. Tim18 deletion mutant exhibited resistance to arsenite...
  5. Tamura Y, Endo T, Iijima M, Sesaki H. Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. J Cell Biol. 2009;185:1029-45 pubmed publisher
    ..Furthermore, we observed synthetic growth defects in ups mutants in combination with loss of Pam17p, which controls the integrity of PAM. Our findings provide a novel molecular mechanism for the regulation of cardiolipin metabolism. ..
  6. Okamoto H, Miyagawa A, Shiota T, Tamura Y, Endo T. Intramolecular disulfide bond of Tim22 protein maintains integrity of the TIM22 complex in the mitochondrial inner membrane. J Biol Chem. 2014;289:4827-38 pubmed publisher
    ..that the disulfide bond of Tim22 stabilizes Tim22 especially at elevated temperature through interactions with Tim18, which are also important for the stability of the TIM22 complex...