Gene Symbol: TIF4631
Description: translation initiation factor eIF4G
Alias: translation initiation factor eIF4G
Species: Saccharomyces cerevisiae S288c
Products:     TIF4631

Top Publications

  1. Clarkson B, Gilbert W, Doudna J. Functional overlap between eIF4G isoforms in Saccharomyces cerevisiae. PLoS ONE. 2010;5:e9114 pubmed publisher
    ..These results suggest that total eIF4G levels, but not isoform-specific functions, determine mRNA-specific translational efficiency...
  2. Kessler S, Sachs A. RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G. Mol Cell Biol. 1998;18:51-7 pubmed
    ..cerevisiae is in translation initiation through an interaction with a 115-amino-acid region of the translation initiation factor eIF4G. The eIF4G-interacting domain of Pab1p was mapped to its second RNA recognition motif (RRM2) in an ..
  3. Dominguez D, Altmann M, Benz J, Baumann U, Trachsel H. Interaction of translation initiation factor eIF4G with eIF4A in the yeast Saccharomyces cerevisiae. J Biol Chem. 1999;274:26720-6 pubmed
    ..These data demonstrate that yeast eIF4A and eIF4G interact and suggest that this interaction is required for translation and cell growth. ..
  4. Tarun S, Sachs A. Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G. EMBO J. 1996;15:7168-77 pubmed
    ..Pab1p only bound to this region when complexed to poly(A). These data support the model that the Pablp-poly(A) tail complex on mRNA can interact with the cap structure via eIF-4G. ..
  5. Hilliker A, Gao Z, Jankowsky E, Parker R. The DEAD-box protein Ded1 modulates translation by the formation and resolution of an eIF4F-mRNA complex. Mol Cell. 2011;43:962-72 pubmed publisher
    ..These results identify Ded1 as a translation initiation factor that assembles and remodels an intermediate complex in translation initiation...
  6. Li Z, Lee I, Moradi E, Hung N, Johnson A, Marcotte E. Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol. 2009;7:e1000213 pubmed publisher
  7. Buchan J, Muhlrad D, Parker R. P bodies promote stress granule assembly in Saccharomyces cerevisiae. J Cell Biol. 2008;183:441-55 pubmed publisher
    ..These observations argue that P bodies are important sites for decisions of mRNA fate and that stress granules, at least in yeast, primarily represent pools of mRNAs stalled in the process of reentry into translation from P bodies. ..
  8. He H, von der Haar T, Singh C, Ii M, Li B, Hinnebusch A, et al. The yeast eukaryotic initiation factor 4G (eIF4G) HEAT domain interacts with eIF1 and eIF5 and is involved in stringent AUG selection. Mol Cell Biol. 2003;23:5431-45 pubmed
    ..These results strongly suggest that the binding of the eIF4G HEAT domain to eIF1 and eIF5 is important for maintaining the integrity of the scanning ribosomal preinitiation complex. ..
  9. Berset C, Zurbriggen A, Djafarzadeh S, Altmann M, Trachsel H. RNA-binding activity of translation initiation factor eIF4G1 from Saccharomyces cerevisiae. RNA. 2003;9:871-80 pubmed
    ..These data suggest that RNA-binding activity is required for eIF4G1 function. ..

More Information


  1. Schäfer T, Strauss D, Petfalski E, Tollervey D, Hurt E. The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes. EMBO J. 2003;22:1370-80 pubmed
    ..Our data provide an initial biochemical map of the pre-40S ribosomal subunit on its path from the nucleolus to the cytoplasm. This pathway involves fewer changes in composition than seen during 60S biogenesis. ..
  2. Neff C, Sachs A. Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally. Mol Cell Biol. 1999;19:5557-64 pubmed
    ..In total, these data indicate that yeast eIF4G and eIF4A physically associate and that this association performs an essential function. ..
  3. Otero L, Ashe M, Sachs A. The yeast poly(A)-binding protein Pab1p stimulates in vitro poly(A)-dependent and cap-dependent translation by distinct mechanisms. EMBO J. 1999;18:3153-63 pubmed
    ..These two proteins bind to translation initiation factor eIF4G and are needed for the translation of capped or polyadenylated mRNA, respectively...
  4. Ptushkina M, von der Haar T, Vasilescu S, Frank R, Birkenhäger R, McCarthy J. Cooperative modulation by eIF4G of eIF4E-binding to the mRNA 5' cap in yeast involves a site partially shared by p20. EMBO J. 1998;17:4798-808 pubmed
    ..The results presented provide a basis for understanding how cycling of eIF4E and eIF4G occurs in yeast translation and explains how p20 can act as a fine, but not as a coarse, regulator of protein synthesis. ..
  5. Tarun S, Wells S, Deardorff J, Sachs A. Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation. Proc Natl Acad Sci U S A. 1997;94:9046-51 pubmed
    ..These data suggest that eIF4G mediates poly(A) tail stimulated translation in vitro, and that Pab1p and the domain encompassing the Pab1p-binding site on eIF4G can compensate for partial loss of eIF4E function in vivo. ..
  6. Goyer C, Altmann M, Lee H, Blanc A, Deshmukh M, Woolford J, et al. TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function. Mol Cell Biol. 1993;13:4860-74 pubmed
    ..In this report, we describe the isolation of yeast gene TIF4631, which encodes p150, and a closely related gene, TIF4632...
  7. Wells S, Hillner P, Vale R, Sachs A. Circularization of mRNA by eukaryotic translation initiation factors. Mol Cell. 1998;2:135-40 pubmed
    ..Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell. ..
  8. Hoyle N, Castelli L, Campbell S, Holmes L, Ashe M. Stress-dependent relocalization of translationally primed mRNPs to cytoplasmic granules that are kinetically and spatially distinct from P-bodies. J Cell Biol. 2007;179:65-74 pubmed
    ..Overall, this study highlights new potential control points in both the regulation of mRNA fate and the global control of translation initiation. ..
  9. Park E, Walker S, Lee J, Rothenburg S, Lorsch J, Hinnebusch A. Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1•PABP mRNPs in vivo. EMBO J. 2011;30:302-16 pubmed publisher
    ..Interestingly, two other RNA-binding regions in eIF4G1 have critical functions downstream of eIF4F•mRNA assembly. ..
  10. Schütz P, Bumann M, Oberholzer A, Bieniossek C, Trachsel H, Altmann M, et al. Crystal structure of the yeast eIF4A-eIF4G complex: an RNA-helicase controlled by protein-protein interactions. Proc Natl Acad Sci U S A. 2008;105:9564-9 pubmed publisher
    ..Conformational changes between eIF4A's closed and open state provide a model for its RNA-helicase activity. ..
  11. De la Cruz J, Iost I, Kressler D, Linder P. The p20 and Ded1 proteins have antagonistic roles in eIF4E-dependent translation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:5201-6 pubmed
    ..cerevisiae. ..
  12. Fortes P, Inada T, Preiss T, Hentze M, Mattaj I, Sachs A. The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation. Mol Cell. 2000;6:191-6 pubmed
    ..Here we report genetic and biochemical evidence that the yeast translation initiation factor eIF4G associates with CBC, and that eIF4E, the eIF4F component that binds both the cap and eIF4G, ..
  13. Das S, Saha U, Das B. Cbc2p, Upf3p and eIF4G are components of the DRN (Degradation of mRNA in the Nucleus) in Saccharomyces cerevisiae. FEMS Yeast Res. 2014;14:922-32 pubmed publisher
    ..These findings clearly establish that CBC2, UPF3, and TIF4631/32 gene products participate in DRN function.
  14. Armakola M, Higgins M, Figley M, Barmada S, Scarborough E, Diaz Z, et al. Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models. Nat Genet. 2012;44:1302-9 pubmed publisher
    ..Our findings provide insight into TDP-43-mediated cytotoxicity and suggest that decreasing Dbr1 activity could be a potential therapeutic approach for ALS. ..
  15. Rajyaguru P, She M, Parker R. Scd6 targets eIF4G to repress translation: RGG motif proteins as a class of eIF4G-binding proteins. Mol Cell. 2012;45:244-54 pubmed publisher
    ..These observations identify the mechanism of Scd6 function through its RGG motif and indicate that eIF4G plays an important role as a scaffolding protein for the recruitment of translation repressors. ..
  16. Rojas M, Farr G, Fernandez C, Lauden L, McCormack J, Wolin S. Yeast Gis2 and its human ortholog CNBP are novel components of stress-induced RNP granules. PLoS ONE. 2012;7:e52824 pubmed publisher
    ..interactions with two proteins involved in mRNA recognition, the poly(A) binding protein and the translation initiation factor eIF4G. We show that Gis2 is a component of two large RNA-protein granules, processing bodies and stress ..
  17. Sobti M, Cubeddu L, Haynes P, Mabbutt B. Engineered rings of mixed yeast Lsm proteins show differential interactions with translation factors and U-rich RNA. Biochemistry. 2010;49:2335-45 pubmed publisher
    ..Our findings suggest Lsm1 and/or Lsm4 can interact with translationally active mRNA. ..
  18. Kafasla P, Barrass J, Thompson E, Fromont Racine M, Jacquier A, Beggs J, et al. Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events. RNA Biol. 2009;6:563-74 pubmed
    As evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae...
  19. Hershey P, McWhirter S, Gross J, Wagner G, Alber T, Sachs A. The Cap-binding protein eIF4E promotes folding of a functional domain of yeast translation initiation factor eIF4G1. J Biol Chem. 1999;274:21297-304 pubmed
    The association of eucaryotic translation initiation factor eIF4G with the cap-binding protein eIF4E establishes a critical link between the mRNA and the ribosome during translation initiation...
  20. Winstall E, Sadowski M, Kuhn U, Wahle E, Sachs A. The Saccharomyces cerevisiae RNA-binding protein Rbp29 functions in cytoplasmic mRNA metabolism. J Biol Chem. 2000;275:21817-26 pubmed
    ..its deletion exacerbates the slow growth phenotype of yeast strains harboring mutations in the eIF4G genes TIF4631 and TIF4632...
  21. Senissar M, Le Saux A, Belgareh Touzé N, Adam C, Banroques J, Tanner N. The DEAD-box helicase Ded1 from yeast is an mRNP cap-associated protein that shuttles between the cytoplasm and nucleus. Nucleic Acids Res. 2014;42:10005-22 pubmed publisher
    ..We conclude that Ded1 is an mRNP cofactor of the cap complex that may function to remodel the different mRNPs and thereby regulate the expression of the mRNAs. ..
  22. Ross D, Saxena M, Altmann M. eIF4E is an important determinant of adhesion and pseudohyphal growth of the yeast S. cerevisiae. PLoS ONE. 2012;7:e50773 pubmed publisher
  23. Park E, Walker S, Zhou F, Lee J, Rajagopal V, Lorsch J, et al. Yeast eukaryotic initiation factor 4B (eIF4B) enhances complex assembly between eIF4A and eIF4G in vivo. J Biol Chem. 2013;288:2340-54 pubmed publisher
    ..These and other findings lead us to propose that yeIF4B acts in vivo to promote eIF4F assembly by enhancing a conformation of the HEAT domain of yeast eIF4G conducive for stable binding to eIF4A. ..
  24. Brandariz Nuñez A, Zeng F, Lam Q, Jin H. Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner. RNA. 2018;24:43-55 pubmed publisher
    ..The functional differences of Sbp1 and RGG repeats alone on transcript-specific translation were observed, and a comparison of the results suggests the importance of remodeling the 5'UTR by RNA-binding proteins in mRNA translation. ..
  25. Singh C, Watanabe R, Chowdhury W, Hiraishi H, Murai M, Yamamoto Y, et al. Sequential eukaryotic translation initiation factor 5 (eIF5) binding to the charged disordered segments of eIF4G and eIF2? stabilizes the 48S preinitiation complex and promotes its shift to the initiation mode. Mol Cell Biol. 2012;32:3978-89 pubmed publisher
  26. Santiveri C, Mirassou Y, Rico Lastres P, Martínez Lumbreras S, Perez Canadillas J. Pub1p C-terminal RRM domain interacts with Tif4631p through a conserved region neighbouring the Pab1p binding site. PLoS ONE. 2011;6:e24481 pubmed publisher
  27. Castelli L, Lui J, Campbell S, Rowe W, Zeef L, Holmes L, et al. Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated. Mol Biol Cell. 2011;22:3379-93 pubmed publisher
  28. Cuenca Bono B, García Molinero V, Pascual García P, García Oliver E, Llopis A, Rodriguez Navarro S. A novel link between Sus1 and the cytoplasmic mRNA decay machinery suggests a broad role in mRNA metabolism. BMC Cell Biol. 2010;11:19 pubmed publisher
    ..These interactions suggest a role for Sus1 in gene expression during cytoplasmic mRNA metabolism in addition to its nuclear function. ..
  29. Tarun S, Sachs A. Binding of eukaryotic translation initiation factor 4E (eIF4E) to eIF4G represses translation of uncapped mRNA. Mol Cell Biol. 1997;17:6876-86 pubmed
    ..These proteins also bind to the translation initiation factor eIF4G and thereby link the mRNA to the general translational apparatus...
  30. McCarthy J. Posttranscriptional control of gene expression in yeast. Microbiol Mol Biol Rev. 1998;62:1492-553 pubmed
  31. Gomar Alba M, Jimenez Marti E, del Olmo M. The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress. BMC Mol Biol. 2012;13:19 pubmed publisher
  32. Kershaw C, Costello J, Castelli L, Talavera D, Rowe W, Sims P, et al. The yeast La related protein Slf1p is a key activator of translation during the oxidative stress response. PLoS Genet. 2015;11:e1004903 pubmed publisher
    ..Together, our results indicate that Slf1p mediates a translational response to oxidative stress via mRNA-specific translational control. ..
  33. Melamed D, YOUNG D, Miller C, Fields S. Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites. PLoS Genet. 2015;11:e1004918 pubmed publisher
    ..Thus, the combined approach of large-scale mutational data and evolutionary conservation can be used to characterize interaction sites at single amino acid resolution. ..
  34. Archer S, Shirokikh N, Hallwirth C, Beilharz T, Preiss T. Probing the closed-loop model of mRNA translation in living cells. RNA Biol. 2015;12:248-54 pubmed publisher
    ..We observed mRNA-specific variation in the extent of closed-loop formation, consistent with a role for polysome topology in the control of gene expression. ..