Gene Symbol: TIF11
Description: Tif11p
Alias: Tif11p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Zheng A, Yamamoto R, Sokabe M, Tanaka I, Yao M. Crystallization and preliminary X-ray crystallographic analysis of eIF5B?N and the eIF5B?N-eIF1A?N complex. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:730-3 pubmed publisher
    ..3?Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 101.9, b = 120.9, c = 132.8?Å. The asymmetric unit was estimated to contain two complex molecules. ..
  2. Fekete C, Applefield D, Blakely S, Shirokikh N, Pestova T, Lorsch J, et al. The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo. EMBO J. 2005;24:3588-601 pubmed
    ..Thus, the OB-fold is crucial for ribosome-binding and the C-terminal domain of eIF1A has eukaryotic-specific functions in TC recruitment and scanning. ..
  3. Passmore L, Schmeing T, Maag D, Applefield D, Acker M, Algire M, et al. The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome. Mol Cell. 2007;26:41-50 pubmed
    ..Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA. ..
  4. Acker M, Shin B, Dever T, Lorsch J. Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining. J Biol Chem. 2006;281:8469-75 pubmed
  5. Martin Marcos P, Nanda J, Luna R, Wagner G, Lorsch J, Hinnebusch A. ?-Hairpin loop of eukaryotic initiation factor 1 (eIF1) mediates 40 S ribosome binding to regulate initiator tRNA(Met) recruitment and accuracy of AUG selection in vivo. J Biol Chem. 2013;288:27546-62 pubmed publisher
    ..Finally, we implicate the unstructured N-terminal tail of eIF1 in blocking rearrangement to the closed conformation in the scanning preinitiation complex. ..
  6. Fekete C, Mitchell S, Cherkasova V, Applefield D, Algire M, Maag D, et al. N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection. EMBO J. 2007;26:1602-14 pubmed
    ..We conclude that tight binding of eIF1A to the PIC is an important determinant of AUG selection and is modulated in opposite directions by residues in the NTT and CTT of eIF1A. ..
  7. Maag D, Fekete C, Gryczynski Z, Lorsch J. A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon. Mol Cell. 2005;17:265-75 pubmed
    ..These rearrangements probably play a role in triggering GTP hydrolysis and committing the complex to downstream events. ..
  8. Gross S, Kinzy T. Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology. Nat Struct Mol Biol. 2005;12:772-8 pubmed
    ..This demonstrates for the first time a direct consequence of eEF1A on cytoskeletal organization in vivo and the physiological significance of this interaction. ..
  9. Möckli N, Deplazes A, Hassa P, Zhang Z, Peter M, Hottiger M, et al. Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques. 2007;42:725-30 pubmed
    ..The cytoY2H system extends existing methods for the detection of protein interactions by providing a convenient solution for screening a wide range of transcriptionally active proteins. ..

More Information


  1. Martin Marcos P, Nanda J, Luna R, Zhang F, Saini A, Cherkasova V, et al. Enhanced eIF1 binding to the 40S ribosome impedes conformational rearrangements of the preinitiation complex and elevates initiation accuracy. RNA. 2014;20:150-67 pubmed publisher
  2. Martin Marcos P, Cheung Y, Hinnebusch A. Functional elements in initiation factors 1, 1A, and 2? discriminate against poor AUG context and non-AUG start codons. Mol Cell Biol. 2011;31:4814-31 pubmed publisher
    ..Although eIF1 autoregulates by discriminating against poor context in yeast and mammals, this mechanism does not prevent eIF1 overproduction in yeast, accounting for the hyperaccuracy phenotype afforded by SUI1 overexpression. ..
  3. Maag D, Algire M, Lorsch J. Communication between eukaryotic translation initiation factors 5 and 1A within the ribosomal pre-initiation complex plays a role in start site selection. J Mol Biol. 2006;356:724-37 pubmed
    ..These data suggest strongly that the interaction between eIF1A and eIF5 is involved in maintaining the fidelity of start codon recognition in vivo. ..
  4. Ozturk S, Vishnu M, Olarewaju O, Starita L, Masison D, Kinzy T. Unique classes of mutations in the Saccharomyces cerevisiae G-protein translation elongation factor 1A suppress the requirement for guanine nucleotide exchange. Genetics. 2006;174:651-63 pubmed
    ..The location of the mutations on the eEF1A-eEF1Balpha structure suggests that their mechanism of suppression may depend on effects on the conserved G-protein elements: the P-loop and NKXD nucleotide-binding element. ..
  5. Luna R, Arthanari H, Hiraishi H, Akabayov B, Tang L, Cox C, et al. The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes. Biochemistry. 2013;52:9510-8 pubmed publisher
    ..These results suggest that the eIF1A-eIF5-CTD interaction during scanning PICs contributes to the maintenance of eIF1 within the open PIC...
  6. Cuchalová L, Kouba T, Herrmannová A, Dányi I, Chiu W, Valásek L. The RNA recognition motif of eukaryotic translation initiation factor 3g (eIF3g) is required for resumption of scanning of posttermination ribosomes for reinitiation on GCN4 and together with eIF3i stimulates linear scanning. Mol Cell Biol. 2010;30:4671-86 pubmed publisher
    ..Together these results implicate g/Tif35 and i/Tif34 in stimulation of linear scanning and, specifically in the case of g/Tif35, also in proper regulation of the GCN4 reinitiation mechanism. ..
  7. McCarthy J. Posttranscriptional control of gene expression in yeast. Microbiol Mol Biol Rev. 1998;62:1492-553 pubmed
  8. Kwon S, Lee I, Kim N, Choi D, Oh Y, Bae S. Translation initiation factor eIF1A possesses RNA annealing activity in its oligonucleotide-binding fold. Biochem Biophys Res Commun. 2007;361:681-6 pubmed
    ..Our results indicate that eIF1A may function as an RNA chaperone, inducing conformational changes in rRNA in the 43S preinitiation complex. ..
  9. Choi S, Olsen D, Roll Mecak A, Martung A, Remo K, Burley S, et al. Physical and functional interaction between the eukaryotic orthologs of prokaryotic translation initiation factors IF1 and IF2. Mol Cell Biol. 2000;20:7183-91 pubmed
  10. ElAntak L, Wagner S, Herrmannová A, Karásková M, Rutkai E, Lukavsky P, et al. The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection. J Mol Biol. 2010;396:1097-116 pubmed publisher
    ..Taken together, we propose that eIF3j/HCR1 closely cooperates with the eIF3b/PRT1 RRM and eIF1A on the ribosome to ensure proper formation of the scanning-arrested conformation required for stringent AUG recognition. ..
  11. Saini A, Nanda J, Lorsch J, Hinnebusch A. Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome. Genes Dev. 2010;24:97-110 pubmed publisher
  12. Olsen D, Savner E, Mathew A, Zhang F, Krishnamoorthy T, Phan L, et al. Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo. EMBO J. 2003;22:193-204 pubmed
    ..We propose a modular organization for eIF1A wherein a core ribosome-binding domain is flanked by flexible segments that mediate interactions with other factors involved in recruitment of TC and release of eIF1A at subunit joining. ..
  13. Algire M, Maag D, Savio P, Acker M, Tarun S, Sachs A, et al. Development and characterization of a reconstituted yeast translation initiation system. RNA. 2002;8:382-97 pubmed
  14. De la Cruz J, Iost I, Kressler D, Linder P. The p20 and Ded1 proteins have antagonistic roles in eIF4E-dependent translation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:5201-6 pubmed
    ..cerevisiae. ..
  15. Fringer J, Acker M, Fekete C, Lorsch J, Dever T. Coupled release of eukaryotic translation initiation factors 5B and 1A from 80S ribosomes following subunit joining. Mol Cell Biol. 2007;27:2384-97 pubmed
    ..Following 80S complex formation, GTP hydrolysis by eIF5B enables the release of both eIF5B and eIF1A, and the ribosome enters the elongation phase of protein synthesis. ..
  16. Hinnebusch A, Asano K, Olsen D, Phan L, Nielsen K, VALASEK L. Study of translational control of eukaryotic gene expression using yeast. Ann N Y Acad Sci. 2004;1038:60-74 pubmed
    ..Thus, apart from its critical role in the starvation response, GCN4 regulation is a valuable tool for dissecting the contributions of multiple translation factors in the eukaryotic initiation pathway. ..