THP1

Summary

Gene Symbol: THP1
Description: Thp1p
Alias: BUD29, Thp1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Chekanova J, Abruzzi K, Rosbash M, Belostotsky D. Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP. RNA. 2008;14:66-77 pubmed
    ..Here we identify Sus1, Thp1, and Sac3 as factors required for the persistent tethering of such foci (dots) to their cognate genes...
  2. Jani D, Lutz S, Marshall N, Fischer T, Köhler A, Ellisdon A, et al. Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export. Mol Cell. 2009;33:727-37 pubmed publisher
    The yeast Sac3:Cdc31:Sus1:Thp1 (TREX-2) complex facilitates the repositioning and association of actively transcribing genes with nuclear pores (NPCs)-"gene gating"-that is central to integrating transcription, processing, and ..
  3. Pascual García P, Govind C, Queralt E, Cuenca Bono B, Llopis A, Chávez S, et al. Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2. Genes Dev. 2008;22:2811-22 pubmed publisher
    ..Our results reveal that Sus1 plays a key role in coordinating gene transcription and mRNA export by working at the interface between the SAGA and TREX2 complexes during transcription elongation. ..
  4. Gallardo M, Aguilera A. A new hyperrecombination mutation identifies a novel yeast gene, THP1, connecting transcription elongation with mitotic recombination. Genetics. 2001;157:79-89 pubmed
    ..We show that Thp1p and two other proteins previously shown to control transcription-associated recombination, Hpr1p and Tho2p, act in ..
  5. González Aguilera C, Tous C, Gómez González B, Huertas P, Luna R, Aguilera A. The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability. Mol Biol Cell. 2008;19:4310-8 pubmed publisher
    ..mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX...
  6. Kilmartin J. Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. J Cell Biol. 2003;162:1211-21 pubmed
    ..The centrosomal human protein containing Sfi1 repeats also binds centrin in the repeat region, showing that this centrin-binding motif is conserved. ..
  7. Ellisdon A, Dimitrova L, Hurt E, Stewart M. Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex. Nat Struct Mol Biol. 2012;19:328-36 pubmed publisher
    ..We report the crystal structure of the Sac3-Thp1-Sem1 segment of Saccharomyces cerevisiae TREX-2 that interfaces with the gene expression machinery...
  8. Faza M, Kemmler S, Jimeno S, González Aguilera C, Aguilera A, Hurt E, et al. Sem1 is a functional component of the nuclear pore complex-associated messenger RNA export machinery. J Cell Biol. 2009;184:833-46 pubmed publisher
    ..Notably, targeting of Thp1, a TREX-2 component, to the NPC is perturbed in a sem1 mutant...
  9. Grund S, Fischer T, Cabal G, Antúnez O, Pérez Ortín J, Hurt E. The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression. J Cell Biol. 2008;182:897-910 pubmed publisher
    ..Our data show that the inner nuclear membrane protein Src1 functions at the interface between subtelomeric gene expression and TREX-dependent messenger RNA export through the nuclear pore complexes. ..

More Information

Publications28

  1. Fischer T, Strasser K, Rácz A, Rodriguez Navarro S, Oppizzi M, Ihrig P, et al. The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. EMBO J. 2002;21:5843-52 pubmed
    ..In vivo, Sac3p forms a stable complex with Thp1p, which was reported to function in transcription elongation...
  2. Köhler A, Schneider M, Cabal G, Nehrbass U, Hurt E. Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export. Nat Cell Biol. 2008;10:707-15 pubmed publisher
    ..Through a separate domain, Sgf73 mediates recruitment of the TREX-2 mRNA export factors Sac3 and Thp1 to SAGA and their stable interaction with Sus1-Cdc31. This latter step is crucial to target TREX-2 to the NPC...
  3. Köhler A, Pascual García P, Llopis A, Zapater M, Posas F, Hurt E, et al. The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11. Mol Biol Cell. 2006;17:4228-36 pubmed
    Sus1 acts in nuclear mRNA export via its association with the nuclear pore-associated Sac3-Thp1-Cdc31 complex. In addition, Sus1 plays a role in transcription through its interaction with the Spt/Ada/Gcn5 acetyltransferase (SAGA) complex...
  4. Strasser K, Masuda S, Mason P, Pfannstiel J, Oppizzi M, Rodriguez Navarro S, et al. TREX is a conserved complex coupling transcription with messenger RNA export. Nature. 2002;417:304-8 pubmed
    ..Our data indicate that the TREX complex has a conserved role in coupling transcription to mRNA export. ..
  5. Fischer T, Rodriguez Navarro S, Pereira G, Rácz A, Schiebel E, Hurt E. Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. Nat Cell Biol. 2004;6:840-8 pubmed
    ..normal conditions, the CID motif recruits Cdc31 and Sus1 (a subunit of the SAGA transcription complex) to the Sac3-Thp1 complex, which functions in mRNA export together with specific nucleoporins at the nuclear basket...
  6. Gallardo M, Luna R, Erdjument Bromage H, Tempst P, Aguilera A. Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism. J Biol Chem. 2003;278:24225-32 pubmed
    ..This result suggests a functional relationship between Thp1p and Nab2p...
  7. Gaillard H, Wellinger R, Aguilera A. A new connection of mRNP biogenesis and export with transcription-coupled repair. Nucleic Acids Res. 2007;35:3893-906 pubmed
    ..In this study we show by molecular DNA repair analysis, that Sub2-Yra1 and Thp1-Sac3, two main mRNA export complexes, are required for efficient TCR in yeast...
  8. Bohn S, Sakata E, Beck F, Pathare G, Schnitger J, Nagy I, et al. Localization of the regulatory particle subunit Sem1 in the 26S proteasome. Biochem Biophys Res Commun. 2013;435:250-4 pubmed publisher
    ..Our structural studies indicate that Sem1 can assume different conformations in different contexts, which supports the idea that Sem1 functions as a molecular glue stabilizing the Rpn3/Rpn7 heterodimer. ..
  9. Aibara S, Bai X, Stewart M. The Sac3 TPR-like region in the Saccharomyces cerevisiae TREX-2 complex is more extensive but independent of the CID region. J Struct Biol. 2016;195:316-324 pubmed publisher
    ..The TREX-2 complex is based on a Sac3 scaffold to which Thp1, Sem1, Cdc31, and Sus1 bind...
  10. Klöckner C, Schneider M, Lutz S, Jani D, Kressler D, Stewart M, et al. Mutational uncoupling of the role of Sus1 in nuclear pore complex targeting of an mRNA export complex and histone H2B deubiquitination. J Biol Chem. 2009;284:12049-56 pubmed publisher
    ..SAGA (Spt-Ada-Gcn5 acetyltransferase) transcriptional co-activator and the mRNA export complex, TREX-2 (Sus1-Sac3-Thp1-Cdc31)...
  11. Gómez Navarro N, Peiró Chova L, Estruch F. Iwr1 facilitates RNA polymerase II dynamics during transcription elongation. Biochim Biophys Acta Gene Regul Mech. 2017;1860:803-811 pubmed publisher
    ..Moreover, absence of Iwr1 causes genome instability that is enhanced by defects in the DNA repair machinery. ..
  12. Jimeno S, Garcia Rubio M, Luna R, Aguilera A. A reduction in RNA polymerase II initiation rate suppresses hyper-recombination and transcription-elongation impairment of THO mutants. Mol Genet Genomics. 2008;280:327-36 pubmed publisher
    ..This study furthers the understanding of the importance of THO in transcription and the maintenance of genome stability. ..
  13. García Oliver E, Pascual García P, García Molinero V, Lenstra T, Holstege F, Rodriguez Navarro S. A novel role for Sem1 and TREX-2 in transcription involves their impact on recruitment and H2B deubiquitylation activity of SAGA. Nucleic Acids Res. 2013;41:5655-68 pubmed publisher
    ..Most of these phenotypes are also found to depend on another TREX-2 subunit, Thp1. These results unveil a new role for Sem1 in the activation of the SAGA-dependent gene GAL1 and influencing H2B ..
  14. Bermejo R, Capra T, Jossen R, Colosio A, Frattini C, Carotenuto W, et al. The replication checkpoint protects fork stability by releasing transcribed genes from nuclear pores. Cell. 2011;146:233-46 pubmed publisher
  15. Holbein S, Wengi A, Decourty L, Freimoser F, Jacquier A, Dichtl B. Cordycepin interferes with 3' end formation in yeast independently of its potential to terminate RNA chain elongation. RNA. 2009;15:837-49 pubmed publisher
    ..Finally, chemical-genetic profiling revealed genome-wide pathways linked to cordycepin activity and identified novel genes involved in poly(A) homeostasis. ..
  16. Skruzny M, Schneider C, Rácz A, Weng J, Tollervey D, Hurt E. An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes. PLoS Biol. 2009;7:e8 pubmed publisher
    ..The data suggest that Swt1 endoribonuclease might be transiently recruited to NPCs to initiate the degradation of defective pre-mRNPs or mRNPs trapped at nuclear periphery in order to avoid their cytoplasmic export and translation...
  17. Proft M, Mas G, de Nadal E, Vendrell A, Noriega N, Struhl K, et al. The stress-activated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress. Mol Cell. 2006;23:241-50 pubmed
    ..Thus, in addition to its various functions during transcriptional initiation, Hog1 behaves as a transcriptional elongation factor that is selective for genes induced upon osmotic stress. ..
  18. Teixeira M, Dujon B, Fabre E. Genome-wide nuclear morphology screen identifies novel genes involved in nuclear architecture and gene-silencing in Saccharomyces cerevisiae. J Mol Biol. 2002;321:551-61 pubmed
    ..Interestingly, these nuclear morphology alterations were associated with chromatin-silencing defects. These genes provide a molecular context to explore the functional link between nuclear architecture and gene silencing. ..
  19. Ni L, Snyder M. A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell. 2001;12:2147-70 pubmed
    ..Genome-wide screens of defined collections of mutants hold significant promise for dissecting many biological processes in yeast. ..