Gene Symbol: TFC1
Description: transcription factor TFIIIC subunit TFC1
Alias: transcription factor TFIIIC subunit TFC1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Leveillard T, Kassavetis G, Geiduschek E. Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem. 1991;266:5162-8 pubmed
    ..Both transcription factors bend DNA, TFIIIB more than TFIIIC. The effective centers of the transcription factor IIIB- and IIIC-induced bends are not in helical phase. ..
  2. Kassavetis G, Braun B, Nguyen L, Geiduschek E. S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors. Cell. 1990;60:235-45 pubmed
    ..Pol III unwinds at least 14 bp of DNA at the SUP4 transcription start in a temperature-dependent process. The unwound DNA segment moves downstream with nascent RNA as a transcription bubble of approximately the same size. ..
  3. Burnol A, Margottin F, Huet J, Almouzni G, Prioleau M, Mechali M, et al. TFIIIC relieves repression of U6 snRNA transcription by chromatin. Nature. 1993;362:475-7 pubmed
    ..This observation resolves an apparent paradox between in vitro and in vivo results concerning the necessity of the downstream B-block element and sheds light on a new role of TFIIIC in gene activation. ..
  4. Swanson R, Conesa C, Lefebvre O, Carles C, Ruet A, Quemeneur E, et al. Isolation of TFC1, a gene encoding one of two DNA-binding subunits of yeast transcription factor tau (TFIIIC). Proc Natl Acad Sci U S A. 1991;88:4887-91 pubmed
    ..obtained peptide sequences from the 95-kDa DNA-binding subunit (tau 95) and cloned the corresponding gene, called TFC1. The gene encodes a polypeptide of calculated Mr 73,500...
  5. Jourdain S, Acker J, Ducrot C, Sentenac A, Lefebvre O. The tau95 subunit of yeast TFIIIC influences upstream and downstream functions of TFIIIC.DNA complexes. J Biol Chem. 2003;278:10450-7 pubmed
    ..The results suggest that tau95 serves as a scaffold critical for tauA.DNA spatial configuration and tauB.DNA stability. ..
  6. Manaud N, Arrebola R, Buffin Meyer B, Lefebvre O, Voss H, Riva M, et al. A chimeric subunit of yeast transcription factor IIIC forms a subcomplex with tau95. Mol Cell Biol. 1998;18:3191-200 pubmed
    ..Interestingly, tau55 was found, together with TFIIIC subunit tau95, in a protein complex which was distinct from TFIIIC and which may play a role in the regulation of PolIII transcription, possibly in relation to cell metabolism. ..
  7. Conesa C, Swanson R, Schultz P, Oudet P, Sentenac A. On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau. J Biol Chem. 1993;268:18047-52 pubmed
    ..of TFIIIC/tau factor were investigated using factor prepared from strains carrying modified forms of TFC1, the gene encoding the 95-kDa TFIIIC/tau subunit (tau 95)...
  8. Pratt Hyatt M, Pai D, Haeusler R, Wozniak G, Good P, Miller E, et al. Mod5 protein binds to tRNA gene complexes and affects local transcriptional silencing. Proc Natl Acad Sci U S A. 2013;110:E3081-9 pubmed publisher
    ..These results are discussed in light of recent results showing that silencing near tRNA genes also requires chromatin modification...
  9. Aye M, Dildine S, Claypool J, Jourdain S, Sandmeyer S. A truncation mutant of the 95-kilodalton subunit of transcription factor IIIC reveals asymmetry in Ty3 integration. Mol Cell Biol. 2001;21:7839-51 pubmed
    ..The orientation bias observed here suggests that even for wild-type Ty3, the protein complexes associated with the long terminal repeats are not equivalent in vivo. ..

More Information


  1. Deprez E, Arrebola R, Conesa C, Sentenac A. A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein. Mol Cell Biol. 1999;19:8042-51 pubmed
    ..Coimmunoprecipitation experiments support a direct interaction between TBP and tau60. It is suggested that tau60 links tauA and tauB domains and participates in TFIIIB assembly via its interaction with TBP. ..
  2. Baker R, Gabrielsen O, Hall B. Effects of tRNATyr point mutations on the binding of yeast RNA polymerase III transcription factor C. J Biol Chem. 1986;261:5275-82 pubmed
    ..Since A block sequences are absolutely required for active tRNA gene transcription, the binding results suggest that the role of A block sequences in transcription is not confined solely to TFIIIC binding. ..
  3. Felts S, Weil P, Chalkley R. Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. Mol Cell Biol. 1990;10:2390-401 pubmed
    ..The patterns of protein-DNA interactions formed on these genes are consistent with these DNAs being assembled into extensive nucleosomal arrays. ..
  4. Chaussivert N, Conesa C, Shaaban S, Sentenac A. Complex interactions between yeast TFIIIB and TFIIIC. J Biol Chem. 1995;270:15353-8 pubmed
    ..We provide evidence that intramolecular interactions mask functional domains in both polypeptides. ..
  5. Lefebvre O, Ruth J, Sentenac A. A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors. J Biol Chem. 1994;269:23374-81 pubmed
    ..A second class of suppressors is not involved in transcription but alleviates the main physiological defects of mutant cells. It includes RPR1 and NOP1, required for the maturation of pre-tRNA and pre-rRNA, respectively. ..
  6. Braun B, Riggs D, Kassavetis G, Geiduschek E. Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genes. Proc Natl Acad Sci U S A. 1989;86:2530-4 pubmed
    ..Complexes assembled from the complete set of purified transcription factors or from a complete transcription system extend over the entire transcription unit together with almost 50 base pairs of 5' flanking sequence. ..
  7. Parsons M, Weil P. Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies. J Biol Chem. 1990;265:5095-103 pubmed
    ..These results suggested that these three polypeptides were tightly associated with one another in solution. ..
  8. Haeusler R, Pratt Hyatt M, Good P, Gipson T, Engelke D. Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes. Genes Dev. 2008;22:2204-14 pubmed publisher
    ..These observations suggest a widespread role for condensin in gene organization and packaging of the interphase yeast nucleus. ..
  9. Tavenet A, Suleau A, Dubreuil G, Ferrari R, Ducrot C, Michaut M, et al. Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription. Proc Natl Acad Sci U S A. 2009;106:14265-70 pubmed publisher
    ..Sub1 was required for optimal Pol III gene transcription in exponentially growing cells. ..
  10. Braun B, Bartholomew B, Kassavetis G, Geiduschek E. Topography of transcription factor complexes on the Saccharomyces cerevisiae 5 S RNA gene. J Mol Biol. 1992;228:1063-77 pubmed
    ..A 90 kDa component that was not previously recognized as an integral part of TFIIIC has been specifically located at the 3' end of the 5 S rRNA gene. ..
  11. Kassavetis G, Riggs D, Negri R, Nguyen L, Geiduschek E. Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes. Mol Cell Biol. 1989;9:2551-66 pubmed
  12. Chedin S, Ferri M, Peyroche G, Andrau J, Jourdain S, Lefebvre O, et al. The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation. Cold Spring Harb Symp Quant Biol. 1998;63:381-9 pubmed
  13. Dumay Odelot H, Marck C, Durrieu Gaillard S, Lefebvre O, Jourdain S, Prochazkova M, et al. Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC. J Biol Chem. 2007;282:17179-89 pubmed