Gene Symbol: TFB3
Description: TFIIH/NER complex subunit TFB3
Alias: RIG2, TFIIH/NER complex subunit TFB3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Pflieger D, Jünger M, Muller M, Rinner O, Lee H, Gehrig P, et al. Quantitative proteomic analysis of protein complexes: concurrent identification of interactors and their state of phosphorylation. Mol Cell Proteomics. 2008;7:326-46 pubmed
    ..It was evaluated on model peptides and proteins and on the complex Ccl1-Kin28-Tfb3 isolated by tandem affinity purification from yeast cells...
  2. Feaver W, Huang W, Gileadi O, Myers L, Gustafsson C, Kornberg R, et al. Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair. J Biol Chem. 2000;275:5941-6 pubmed
    A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and ..
  3. Takagi Y, Komori H, Chang W, Hudmon A, Erdjument Bromage H, Tempst P, et al. Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH. J Biol Chem. 2003;278:43897-900 pubmed
    ..b>Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin ..
  4. Akhtar M, Heidemann M, Tietjen J, Zhang D, Chapman R, Eick D, et al. TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II. Mol Cell. 2009;34:387-93 pubmed publisher
    ..These bivalent CTD marks, in concert with cues within nascent transcripts, specify the cotranscriptional engagement of the relevant RNA processing machinery. ..
  5. Kim M, Suh H, Cho E, Buratowski S. Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7. J Biol Chem. 2009;284:26421-6 pubmed publisher
    ..The basal factor TFIIH can phosphorylate Ser-7 in vitro and is necessary for Ser-7(P) in vivo. Interestingly, deletion of the CTD Ser-5(P) phosphatase Rtr1 leads to an increase in Ser-5(P) but not Ser-7(P). ..
  6. Feaver W, Henry N, Wang Z, Wu X, Svejstrup J, Bushnell D, et al. Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH. Homology to human cyclin-dependent kinase activating kinase and IIH subunits. J Biol Chem. 1997;272:19319-27 pubmed
    Genes for the Tfb2, Tfb3, and Tfb4 subunits of yeast RNA polymerase transcription factor IIH (TFIIH) are described. All three genes are essential for cell viability, and antibodies against Tfb3 specifically inhibit transcription in vitro...
  7. Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus D, Le Dez G, et al. Protein quality control at the inner nuclear membrane. Nature. 2014;516:410-3 pubmed publisher
    ..We show that the Asi ubiquitin ligase is involved in degradation of mislocalized integral membrane proteins, thus acting to maintain and safeguard the identity of the INM. ..
  8. Bieganowski P, Garrison P, Hodawadekar S, Faye G, Barnes L, Brenner C. Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. J Biol Chem. 2002;277:10852-60 pubmed publisher
    ..Loss of Hnt1 enzyme activity also leads to hypersensitivity to mutations in Ccl1, Tfb3, and Kin28, which constitute the TFIIK kinase subcomplex of general transcription factor TFIIH and to mutations in ..
  9. Keogh M, Cho E, Podolny V, Buratowski S. Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation. Mol Cell Biol. 2002;22:1288-97 pubmed
    ..Here we show that a trimeric complex of Kin28-Ccl1-Tfb3 exists in yeast extracts. Several Kin28 point mutants that are defective in CTD phosphorylation were created...

More Information


  1. Yang C, Khaperskyy D, Hou M, Ponticelli A. Improved methods for expression and purification of Saccharomyces cerevisiae TFIIF and TFIIH; identification of a functional Escherichia coli promoter and internal translation initiation within the N-terminal coding region of the TFIIF TFG1 subunit. Protein Expr Purif. 2010;70:172-8 pubmed publisher
    ..We also report conditions for the efficient two-step tandem affinity purification (TAP) of holo-TFIIH, core TFIIH and TFIIK complexes from yeast whole cell extracts. ..
  2. Ohtsuki K, Kasahara K, Shirahige K, Kokubo T. Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters. Nucleic Acids Res. 2010;38:1805-20 pubmed publisher
    ..Here, we examine the localization profiles of a complete set of Tafs, Gcn5, Bur6/Ncb2, Sua7, Tfa2, Tfg1, Tfb3 and Rpb1, on chromosomes III, IV and V by chromatin immunoprecipitation (ChIP)-chip analysis in wild-type and taf1-..
  3. Rabut G, Le Dez G, Verma R, Makhnevych T, Knebel A, Kurz T, et al. The TFIIH subunit Tfb3 regulates cullin neddylation. Mol Cell. 2011;43:488-95 pubmed publisher
    ..dependent on the ubiquitin-conjugating enzyme Ubc4, while efficient neddylation involves the RING domain protein Tfb3, a subunit of the transcription factor TFIIH...
  4. Gibbons B, Brignole E, Azubel M, Murakami K, Voss N, Bushnell D, et al. Subunit architecture of general transcription factor TFIIH. Proc Natl Acad Sci U S A. 2012;109:1949-54 pubmed publisher
    ..In this picture, the key catalytic components of TFIIH, the Ssl2 ATPase/helicase and the Kin28 protein kinase are in proximity to their targets, downstream promoter DNA and the RNA polymerase C-terminal domain. ..
  5. Warfield L, Luo J, Ranish J, Hahn S. Function of Conserved Topological Regions within the Saccharomyces cerevisiae Basal Transcription Factor TFIIH. Mol Cell Biol. 2016;36:2464-75 pubmed publisher
    ..e., Ssl1, Tfb4, and Tfb2, in the DNA translocase subunit Ssl2, and in the kinase module subunit Tfb3. Our results are consistent with previously predicted roles for the Tfb2 Hub, Ssl2 Lock, and Tfb3 Latch regions, ..
  6. Svejstrup J, Feaver W, Kornberg R. Subunits of yeast RNA polymerase II transcription factor TFIIH encoded by the CCL1 gene. J Biol Chem. 1996;271:643-5 pubmed
    ..In all likelihood, these two subunits individually form cyclin-dependent kinase/cyclin dimers with Kin28 protein, a key enzyme in phosphorylation of the C-terminal domain of RNA polymerase II concomitant with transcription. ..
  7. Luo J, Cimermancic P, Viswanath S, Ebmeier C, Kim B, Dehecq M, et al. Architecture of the Human and Yeast General Transcription and DNA Repair Factor TFIIH. Mol Cell. 2015;59:794-806 pubmed publisher
    ..We also reveal the structural basis for defects in patients with xeroderma pigmentosum and trichothiodystrophy, with mutations found at the interface between the p62 Anchor region and the XPD subunit. ..
  8. Murakami K, Gibbons B, Davis R, Nagai S, Liu X, Robinson P, et al. Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation. Proc Natl Acad Sci U S A. 2012;109:4816-21 pubmed publisher
    ..Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes. ..
  9. Chang W, Kornberg R. Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH. Cell. 2000;102:609-13 pubmed
    ..The structure is based on a ring of three subunits, Tfb1, Tfb2, and Tfb3, to which are appended several functional moieties: Rad3, bridged to Tfb1 by SsI1; SsI2, known to interact with ..
  10. Faye G, Simon M, Valay J, Fesquet D, Facca C. Rig2, a RING finger protein that interacts with the Kin28/Ccl1 CTD kinase in yeast. Mol Gen Genet. 1997;255:460-6 pubmed
    ..The corresponding gene, RIG2, encodes a predicted RING finger protein...
  11. Wade P, Jaehning J. Transcriptional corepression in vitro: a Mot1p-associated form of TATA-binding protein is required for repression by Leu3p. Mol Cell Biol. 1996;16:1641-8 pubmed
    ..In addition, a mutation in the Mot1 gene leads to partial derepression of the Leu3p-dependent LEU2 promoter. These in vivo and in vitro observations define a role for Mot1p as a transcriptional corepressor. ..