TFB2

Summary

Gene Symbol: TFB2
Description: TFIIH/NER complex subunit TFB2
Alias: TFIIH/NER complex subunit TFB2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Feaver W, Henry N, Wang Z, Wu X, Svejstrup J, Bushnell D, et al. Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH. Homology to human cyclin-dependent kinase activating kinase and IIH subunits. J Biol Chem. 1997;272:19319-27 pubmed
    Genes for the Tfb2, Tfb3, and Tfb4 subunits of yeast RNA polymerase transcription factor IIH (TFIIH) are described. All three genes are essential for cell viability, and antibodies against Tfb3 specifically inhibit transcription in vitro...
  2. Feaver W, Huang W, Gileadi O, Myers L, Gustafsson C, Kornberg R, et al. Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair. J Biol Chem. 2000;275:5941-6 pubmed
    ..yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision ..
  3. Takagi Y, Komori H, Chang W, Hudmon A, Erdjument Bromage H, Tempst P, et al. Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH. J Biol Chem. 2003;278:43897-900 pubmed
    ..This reassignment of subunits resolves a longstanding discrepancy between yeast and human TFIIH complexes. ..
  4. Chang W, Kornberg R. Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH. Cell. 2000;102:609-13 pubmed
    ..The structure is based on a ring of three subunits, Tfb1, Tfb2, and Tfb3, to which are appended several functional moieties: Rad3, bridged to Tfb1 by SsI1; SsI2, known to ..
  5. Ranish J, Hahn S, Lu Y, Yi E, Li X, Eng J, et al. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Nat Genet. 2004;36:707-13 pubmed
    ..The identification of a new, evolutionarily conserved, core TFIIH subunit is essential for our understanding of TFIIH function in transcription, DNA repair and human disease. ..
  6. Wade P, Jaehning J. Transcriptional corepression in vitro: a Mot1p-associated form of TATA-binding protein is required for repression by Leu3p. Mol Cell Biol. 1996;16:1641-8 pubmed
    ..In addition, a mutation in the Mot1 gene leads to partial derepression of the Leu3p-dependent LEU2 promoter. These in vivo and in vitro observations define a role for Mot1p as a transcriptional corepressor. ..
  7. Sun L, Johnston S, Kodadek T. Physical association of the APIS complex and general transcription factors. Biochem Biophys Res Commun. 2002;296:991-9 pubmed
    ..These data add to the growing body of evidence that the APIS complex has a role in transcription, independent of its role in proteolysis and, furthermore, argues that it functions in association with the general transcription complex. ..
  8. Duan R, Rhie B, Ryu H, Ahn S. The RNA polymerase II Rpb4/7 subcomplex regulates cellular lifespan through an mRNA decay process. Biochem Biophys Res Commun. 2013;441:266-70 pubmed
  9. Takagi Y, Masuda C, Chang W, Komori H, Wang D, Hunter T, et al. Ubiquitin ligase activity of TFIIH and the transcriptional response to DNA damage. Mol Cell. 2005;18:237-43 pubmed
    ..Rather, it correlates with reduced transcriptional induction of genes involved in DNA repair, suggesting that the E3 Ub ligase activity of TFIIH mediates the transcriptional response to DNA damage. ..

More Information

Publications24

  1. Akhtar M, Heidemann M, Tietjen J, Zhang D, Chapman R, Eick D, et al. TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II. Mol Cell. 2009;34:387-93 pubmed publisher
    ..These bivalent CTD marks, in concert with cues within nascent transcripts, specify the cotranscriptional engagement of the relevant RNA processing machinery. ..
  2. Gibbons B, Brignole E, Azubel M, Murakami K, Voss N, Bushnell D, et al. Subunit architecture of general transcription factor TFIIH. Proc Natl Acad Sci U S A. 2012;109:1949-54 pubmed publisher
    ..Consistency among all the structures establishes the location of the "minimal core" subunits (Ssl1, Tfb1, Tfb2, Tfb4, and Tfb5), and additional densities can be specifically attributed to Rad3, Ssl2, and the TFIIK trimer...
  3. Estruch F, Cole C. An early function during transcription for the yeast mRNA export factor Dbp5p/Rat8p suggested by its genetic and physical interactions with transcription factor IIH components. Mol Biol Cell. 2003;14:1664-76 pubmed
    ..Together, our results suggest a nuclear role for Dbp5 during the early steps of transcription. ..
  4. Kim M, Suh H, Cho E, Buratowski S. Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7. J Biol Chem. 2009;284:26421-6 pubmed publisher
    ..The basal factor TFIIH can phosphorylate Ser-7 in vitro and is necessary for Ser-7(P) in vivo. Interestingly, deletion of the CTD Ser-5(P) phosphatase Rtr1 leads to an increase in Ser-5(P) but not Ser-7(P). ..
  5. Yang C, Khaperskyy D, Hou M, Ponticelli A. Improved methods for expression and purification of Saccharomyces cerevisiae TFIIF and TFIIH; identification of a functional Escherichia coli promoter and internal translation initiation within the N-terminal coding region of the TFIIF TFG1 subunit. Protein Expr Purif. 2010;70:172-8 pubmed publisher
    ..We also report conditions for the efficient two-step tandem affinity purification (TAP) of holo-TFIIH, core TFIIH and TFIIK complexes from yeast whole cell extracts. ..
  6. Murakami K, Gibbons B, Davis R, Nagai S, Liu X, Robinson P, et al. Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation. Proc Natl Acad Sci U S A. 2012;109:4816-21 pubmed publisher
    ..Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes. ..
  7. Duan R, Rhie B, Ryu H, Ahn S. The RNA polymerase II Rpb4/7 subcomplex regulates cellular lifespan through an mRNA decay process. Biochem Biophys Res Commun. 2013;: pubmed publisher
  8. Kainov D, Selth L, Svejstrup J, Egly J, Poterzsman A. Interacting partners of the Tfb2 subunit from yeast TFIIH. DNA Repair (Amst). 2010;9:33-9 pubmed publisher
    ..Here we focused on interplay between the Ssl2, Tfb2 and Tfb5 subunits of TFIIH from Saccharomyces cerevisiae...
  9. Kainov D, Cura V, Vitorino M, Nierengarten H, Poussin P, Kieffer B, et al. Structure determination of the minimal complex between Tfb5 and Tfb2, two subunits of the yeast transcription/DNA-repair factor TFIIH: a retrospective study. Acta Crystallogr D Biol Crystallogr. 2010;66:745-55 pubmed publisher
    Tfb5 interacts with the Tfb2 subunit of the general transcription factor TFIIH to ensure efficient nucleotide-excision repair in eukaryotes...
  10. Luo J, Cimermancic P, Viswanath S, Ebmeier C, Kim B, Dehecq M, et al. Architecture of the Human and Yeast General Transcription and DNA Repair Factor TFIIH. Mol Cell. 2015;59:794-806 pubmed publisher
    ..We also reveal the structural basis for defects in patients with xeroderma pigmentosum and trichothiodystrophy, with mutations found at the interface between the p62 Anchor region and the XPD subunit. ..
  11. Warfield L, Luo J, Ranish J, Hahn S. Function of Conserved Topological Regions within the Saccharomyces cerevisiae Basal Transcription Factor TFIIH. Mol Cell Biol. 2016;36:2464-75 pubmed publisher
    ..We systematically tested the function of these regions in three TFIIH core module subunits, i.e., Ssl1, Tfb4, and Tfb2, in the DNA translocase subunit Ssl2, and in the kinase module subunit Tfb3...
  12. Kainov D, Vitorino M, Cavarelli J, Poterszman A, Egly J. Structural basis for group A trichothiodystrophy. Nat Struct Mol Biol. 2008;15:980-4 pubmed
    ..the crystal structure of a minimal complex between Tfb5, the yeast ortholog of p8, and the C-terminal domain of Tfb2, the yeast p52 subunit of TFIIH...
  13. Svejstrup J, Feaver W, Kornberg R. Subunits of yeast RNA polymerase II transcription factor TFIIH encoded by the CCL1 gene. J Biol Chem. 1996;271:643-5 pubmed
    ..In all likelihood, these two subunits individually form cyclin-dependent kinase/cyclin dimers with Kin28 protein, a key enzyme in phosphorylation of the C-terminal domain of RNA polymerase II concomitant with transcription. ..
  14. Zhou Y, Kou H, Wang Z. Tfb5 interacts with Tfb2 and facilitates nucleotide excision repair in yeast. Nucleic Acids Res. 2007;35:861-71 pubmed
    ..Lacking a DNA binding activity, Tfb5 was found to interact with the core TFIIH subunit Tfb2, but not with other NER proteins...
  15. Moriel Carretero M, Tous C, Aguilera A. Control of the function of the transcription and repair factor TFIIH by the action of the cochaperone Ydj1. Proc Natl Acad Sci U S A. 2011;108:15300-5 pubmed publisher
    ..Our results provide evidence for a role of chaperones in NER and transcription, with implications in cancer and TFIIH-associated syndromes. ..