TEF2

Summary

Gene Symbol: TEF2
Description: translation elongation factor EF-1 alpha
Alias: translation elongation factor EF-1 alpha
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. McCarthy J. Posttranscriptional control of gene expression in yeast. Microbiol Mol Biol Rev. 1998;62:1492-553 pubmed
  2. Grosshans H, Hurt E, Simos G. An aminoacylation-dependent nuclear tRNA export pathway in yeast. Genes Dev. 2000;14:830-40 pubmed
    ..Our data show that tRNA aminoacylation and eEF-1A are required for efficient nuclear tRNA export in yeast and suggest coordination between the protein translation and the nuclear tRNA processing and transport machineries. ..
  3. McGuire A, Mangroo D. Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery. EMBO J. 2007;26:288-300 pubmed
    ..cerevisiae. They also suggest that Cex1p collects aminoacyl-tRNAs from the nuclear export receptors at the cytoplasmic side of the nuclear pore complex, and transfers them to eEF-1A using a channelling mechanism. ..
  4. Dewe J, Whipple J, Chernyakov I, Jaramillo L, Phizicky E. The yeast rapid tRNA decay pathway competes with elongation factor 1A for substrate tRNAs and acts on tRNAs lacking one or more of several modifications. RNA. 2012;18:1886-96 pubmed publisher
    ..These results demonstrate that RTD interacts with the translation machinery and acts widely on hypomodified tRNAs. ..
  5. Sobti M, Cubeddu L, Haynes P, Mabbutt B. Engineered rings of mixed yeast Lsm proteins show differential interactions with translation factors and U-rich RNA. Biochemistry. 2010;49:2335-45 pubmed publisher
    ..Our findings suggest Lsm1 and/or Lsm4 can interact with translationally active mRNA. ..
  6. Murthi A, Shaheen H, Huang H, Preston M, Lai T, Phizicky E, et al. Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:639-49 pubmed publisher
    ..Finally, we implicate Tef1, the yeast orthologue of translation elongation factor eEF1A, in the tRNA reexport process and show that its subcellular distribution between the nucleus and cytoplasm is dependent upon Mtr10 and Msn5. ..
  7. Pedersen L, Andersen G, Knudsen C, Kinzy T, Nyborg J. Crystallization of the yeast elongation factor complex eEF1A-eEF1B alpha. Acta Crystallogr D Biol Crystallogr. 2001;57:159-61 pubmed
  8. Roberts T, Kobor M, Bastin Shanower S, Ii M, Horte S, Gin J, et al. Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Mol Biol Cell. 2006;17:539-48 pubmed
  9. Laxman S, Tu B. Multiple TORC1-associated proteins regulate nitrogen starvation-dependent cellular differentiation in Saccharomyces cerevisiae. PLoS ONE. 2011;6:e26081 pubmed publisher
    ..Our studies also suggest the CEN.PK strain background of S. cerevisiae may be particularly useful for investigations of nitrogen starvation-induced diploid pseudohyphal growth. ..

More Information

Publications39

  1. Visweswaraiah J, Lageix S, Castilho B, Izotova L, Kinzy T, Hinnebusch A, et al. Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 protein C terminus and inhibits Gcn2 activity. J Biol Chem. 2011;286:36568-79 pubmed publisher
    ..These findings implicate eEF1A in the intricate regulation of Gcn2 and amino acid homeostasis. ..
  2. Kovalchuke O, Kambampati R, Pladies E, Chakraburtty K. Competition and cooperation amongst yeast elongation factors. Eur J Biochem. 1998;258:986-93 pubmed
    ..These results strongly suggest that the stimulatory effect of EF-3 on the ternary complex binding to yeast ribosomes involves a direct interaction between EF-1 alpha and EF-3. ..
  3. Honey S, Schneider B, Schieltz D, Yates J, Futcher B. A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex. Nucleic Acids Res. 2001;29:E24 pubmed
    ..Associated proteins were identified using mass spectrometry. These included the known associated proteins Cdc28, Sic1 and Cks1. Several other proteins were found including the 70 kDa chaperone, Ssa1. ..
  4. Nekrasov V, Smith M, Peak Chew S, Kilmartin J. Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:4931-46 pubmed
    ..These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe. ..
  5. Jao D, Chen K. Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex. J Cell Biochem. 2006;97:583-98 pubmed
  6. Cottrelle P, Cool M, Thuriaux P, Price V, Thiele D, Buhler J, et al. Either one of the two yeast EF-1 alpha genes is required for cell viability. Curr Genet. 1985;9:693-7 pubmed
    Two genes, TEF1 and TEF2, encode the protein elongation factor EF-1 alpha in the yeast Saccharomyces cerevisiae...
  7. Gromadski K, Schümmer T, Strømgaard A, Knudsen C, Kinzy T, Rodnina M. Kinetics of the interactions between yeast elongation factors 1A and 1Balpha, guanine nucleotides, and aminoacyl-tRNA. J Biol Chem. 2007;282:35629-37 pubmed
    ..eEF1A.GTP binds Phe-tRNA(Phe) with a K(d) of 3 nm, whereas eEF1A.GDP shows no significant binding, indicating that eEF1A has similar tRNA binding properties as its prokaryotic homolog, EF-Tu. ..
  8. Gross S, Kinzy T. Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology. Nat Struct Mol Biol. 2005;12:772-8 pubmed
    ..This demonstrates for the first time a direct consequence of eEF1A on cytoskeletal organization in vivo and the physiological significance of this interaction. ..
  9. Kinzy T, Woolford J. Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta. Genetics. 1995;141:481-9 pubmed
    ..a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha...
  10. Umikawa M, Tanaka K, Kamei T, Shimizu K, Imamura H, Sasaki T, et al. Interaction of Rho1p target Bni1p with F-actin-binding elongation factor 1alpha: implication in Rho1p-regulated reorganization of the actin cytoskeleton in Saccharomyces cerevisiae. Oncogene. 1998;16:2011-6 pubmed
    ..These results suggest that the Rho1p-Bni1p system regulates reorganization of the actin cytoskeleton through the interaction with both EF1alpha and profilin. ..
  11. Ranish J, Yi E, Leslie D, Purvine S, Goodlett D, Eng J, et al. The study of macromolecular complexes by quantitative proteomics. Nat Genet. 2003;33:349-55 pubmed
  12. Möckli N, Deplazes A, Hassa P, Zhang Z, Peter M, Hottiger M, et al. Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques. 2007;42:725-30 pubmed
    ..The cytoY2H system extends existing methods for the detection of protein interactions by providing a convenient solution for screening a wide range of transcriptionally active proteins. ..
  13. Chakraburtty K, Triana Alonso F. Yeast elongation factor 3: structure and function. Biol Chem. 1998;379:831-40 pubmed
    ..EF-3 function is dependent on ATP hydrolysis. The existence of functional homologs of EF-3 in higher eukaryotes is still an open question. Further investigations are needed to settle this issue. ..
  14. Nagashima K, Kasai M, Nagata S, Kaziro Y. Structure of the two genes coding for polypeptide chain elongation factor 1 alpha (EF-1 alpha) from Saccharomyces cerevisiae. Gene. 1986;45:265-73 pubmed
    ..The sequence which commonly exists in the 5'-flanking regions of ribosomal protein genes of S. cerevisiae was also present in the two EF1 alpha genes. ..
  15. Drummond S, Hildyard J, Firczuk H, Reamtong O, Li N, Kannambath S, et al. Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes. Nucleic Acids Res. 2011;39:7764-74 pubmed publisher
    ..This reveals a new dimension to the relationship between translation activity and interactions between mRNA, the translation machinery and decapping activator proteins. ..
  16. Daugeron M, Prouteau M, Lacroute F, Seraphin B. The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1. Nucleic Acids Res. 2011;39:2221-33 pubmed publisher
  17. Pittman Y, Kandl K, Lewis M, Valente L, Kinzy T. Coordination of eukaryotic translation elongation factor 1A (eEF1A) function in actin organization and translation elongation by the guanine nucleotide exchange factor eEF1Balpha. J Biol Chem. 2009;284:4739-47 pubmed publisher
    ..The consequences of the overlapping functions in this eEF1A domain and its unique differences from the bacterial homologs provide a novel function for eEF1Balpha to balance the dual roles in actin bundling and protein synthesis. ..
  18. Mishra A, Gangwani L, Davis R, Lambright D. Structural insights into the interaction of the evolutionarily conserved ZPR1 domain tandem with eukaryotic EF1A, receptors, and SMN complexes. Proc Natl Acad Sci U S A. 2007;104:13930-5 pubmed
    ..Structural differences between the ZPR1 domains contribute to the observed functional divergence and provide evidence for distinct modalities of interaction with eEF1A and survival motor neuron complexes. ..
  19. Aström S, Nordlund M, Erickson F, Hannig E, Byström A. Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiae. Mol Gen Genet. 1999;261:967-76 pubmed
    ..On the other hand, introduction of a high-copy-number plasmid carrying the TEF2 gene, which encodes the eukaryotic elongation factor 1alpha (eEF-1alpha), into rit1 null strains with two intact ..
  20. Lipson R, Webb K, Clarke S. Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae. Arch Biochem Biophys. 2010;500:137-43 pubmed publisher
    ..We suggest that YHL039W (now designated EFM1 for elongation factor methyltransferase 1) and YIL064W/SEE1 encode distinct eEF1A methyltransferases that respectively monomethylate and dimethylate this protein at lysine residues. ..
  21. Calvert M, Keck K, Ptak C, Shabanowitz J, Hunt D, Pemberton L. Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol. 2008;28:1313-25 pubmed
    ..In conclusion, our data show that Nap1 phosphorylation by CK2 appears to regulate Nap1 localization and is required for normal progression through S phase. ..
  22. Naticchia M, Brown H, Garcia F, Lamade A, Justice S, Herrin R, et al. Bifunctional electrophiles cross-link thioredoxins with redox relay partners in cells. Chem Res Toxicol. 2013;26:490-7 pubmed publisher
    ..Taken together, our results indicate that bifunctional electrophiles potentially disrupt redox homeostasis in yeast and human cells by forming cross-linked complexes between thioredoxins and their redox partners. ..
  23. Bodman J, Yang Y, Logan M, Eitzen G. Yeast translation elongation factor-1A binds vacuole-localized Rho1p to facilitate membrane integrity through F-actin remodeling. J Biol Chem. 2015;290:4705-16 pubmed publisher
    ..Here, we identified eEF1A as a vacuolar Rho1p-interacting protein. eEF1A (encoded by the TEF1 and TEF2 genes in yeast) is an aminoacyl-tRNA transferase needed during protein translation...
  24. Kwapisz M, Cholbinski P, Hopper A, Rousset J, Zoladek T. Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae. RNA. 2005;11:1710-8 pubmed
    ..This defect, also reversed by overproduction of the elongation factor eEF1A, may be the primary reason for altered translational decoding accuracy. ..
  25. Carr Schmid A, Durko N, Cavallius J, Merrick W, Kinzy T. Mutations in a GTP-binding motif of eukaryotic elongation factor 1A reduce both translational fidelity and the requirement for nucleotide exchange. J Biol Chem. 1999;274:30297-302 pubmed
    ..Additionally, eEF1A mutations that suppress the requirement for guanine nucleotide exchange may not effectively perform all the functions of eEF1A in vivo. ..
  26. Doyle A, Crosby S, Burton D, Lilley F, Murphy M. Actin bundling and polymerisation properties of eukaryotic elongation factor 1 alpha (eEF1A), histone H2A-H2B and lysozyme in vitro. J Struct Biol. 2011;176:370-8 pubmed publisher
  27. Krokowski D, Tchorzewski M, Boguszewska A, McKay A, Maslen S, Robinson C, et al. Elevated copy number of L-A virus in yeast mutant strains defective in ribosomal stalk. Biochem Biophys Res Commun. 2007;355:575-80 pubmed
  28. Waller T, Lee S, Sattlegger E. Evidence that Yih1 resides in a complex with ribosomes. FEBS J. 2012;279:1761-76 pubmed publisher
    ..Close physical proximity of Yih1 to the Gcn1-Gcn2-ribosome complex would allow cells to quickly inhibit Gcn2 whenever or wherever necessary. ..
  29. Munshi R, Kandl K, Carr Schmid A, Whitacre J, Adams A, Kinzy T. Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast. Genetics. 2001;157:1425-36 pubmed
    ..As the ability of eEF1A to bind and bundle actin is conserved in yeast, these results link the established ability of eEF1A to bind and bundle actin in vitro with nontranslational roles for the protein in vivo. ..
  30. Huang H, Hopper A. In vivo biochemical analyses reveal distinct roles of β-importins and eEF1A in tRNA subcellular traffic. Genes Dev. 2015;29:772-83 pubmed publisher
    ..Assembly and/or stability of this quaternary complex requires Tef1/2, thereby facilitating efficient re-export of aminoacylated tRNAs to the cytoplasm. ..