Genomes and Genes
Gene Symbol: TEF2
Description: translation elongation factor EF-1 alpha
Alias: translation elongation factor EF-1 alpha
Species: Saccharomyces cerevisiae S288c
- Visweswaraiah J, Lageix S, Castilho B, Izotova L, Kinzy T, Hinnebusch A, et al. Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 protein C terminus and inhibits Gcn2 activity. J Biol Chem. 2011;286:36568-79 pubmed publisher..These findings implicate eEF1A in the intricate regulation of Gcn2 and amino acid homeostasis. ..
- Kovalchuke O, Kambampati R, Pladies E, Chakraburtty K. Competition and cooperation amongst yeast elongation factors. Eur J Biochem. 1998;258:986-93 pubmed..These results strongly suggest that the stimulatory effect of EF-3 on the ternary complex binding to yeast ribosomes involves a direct interaction between EF-1 alpha and EF-3. ..
- Honey S, Schneider B, Schieltz D, Yates J, Futcher B. A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex. Nucleic Acids Res. 2001;29:E24 pubmed..Associated proteins were identified using mass spectrometry. These included the known associated proteins Cdc28, Sic1 and Cks1. Several other proteins were found including the 70 kDa chaperone, Ssa1. ..
- Nekrasov V, Smith M, Peak Chew S, Kilmartin J. Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:4931-46 pubmed..These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe. ..
- Jao D, Chen K. Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex. J Cell Biochem. 2006;97:583-98 pubmed
- Cottrelle P, Cool M, Thuriaux P, Price V, Thiele D, Buhler J, et al. Either one of the two yeast EF-1 alpha genes is required for cell viability. Curr Genet. 1985;9:693-7 pubmedTwo genes, TEF1 and TEF2, encode the protein elongation factor EF-1 alpha in the yeast Saccharomyces cerevisiae...
- Gromadski K, Schümmer T, Strømgaard A, Knudsen C, Kinzy T, Rodnina M. Kinetics of the interactions between yeast elongation factors 1A and 1Balpha, guanine nucleotides, and aminoacyl-tRNA. J Biol Chem. 2007;282:35629-37 pubmed..eEF1A.GTP binds Phe-tRNA(Phe) with a K(d) of 3 nm, whereas eEF1A.GDP shows no significant binding, indicating that eEF1A has similar tRNA binding properties as its prokaryotic homolog, EF-Tu. ..
- Gross S, Kinzy T. Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology. Nat Struct Mol Biol. 2005;12:772-8 pubmed..This demonstrates for the first time a direct consequence of eEF1A on cytoskeletal organization in vivo and the physiological significance of this interaction. ..
- Kinzy T, Woolford J. Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta. Genetics. 1995;141:481-9 pubmed..a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha...
- Umikawa M, Tanaka K, Kamei T, Shimizu K, Imamura H, Sasaki T, et al. Interaction of Rho1p target Bni1p with F-actin-binding elongation factor 1alpha: implication in Rho1p-regulated reorganization of the actin cytoskeleton in Saccharomyces cerevisiae. Oncogene. 1998;16:2011-6 pubmed..These results suggest that the Rho1p-Bni1p system regulates reorganization of the actin cytoskeleton through the interaction with both EF1alpha and profilin. ..
- Ranish J, Yi E, Leslie D, Purvine S, Goodlett D, Eng J, et al. The study of macromolecular complexes by quantitative proteomics. Nat Genet. 2003;33:349-55 pubmed
- Möckli N, Deplazes A, Hassa P, Zhang Z, Peter M, Hottiger M, et al. Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques. 2007;42:725-30 pubmed..The cytoY2H system extends existing methods for the detection of protein interactions by providing a convenient solution for screening a wide range of transcriptionally active proteins. ..
- Chakraburtty K, Triana Alonso F. Yeast elongation factor 3: structure and function. Biol Chem. 1998;379:831-40 pubmed..EF-3 function is dependent on ATP hydrolysis. The existence of functional homologs of EF-3 in higher eukaryotes is still an open question. Further investigations are needed to settle this issue. ..
- Nagashima K, Kasai M, Nagata S, Kaziro Y. Structure of the two genes coding for polypeptide chain elongation factor 1 alpha (EF-1 alpha) from Saccharomyces cerevisiae. Gene. 1986;45:265-73 pubmed..The sequence which commonly exists in the 5'-flanking regions of ribosomal protein genes of S. cerevisiae was also present in the two EF1 alpha genes. ..
- Drummond S, Hildyard J, Firczuk H, Reamtong O, Li N, Kannambath S, et al. Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes. Nucleic Acids Res. 2011;39:7764-74 pubmed publisher..This reveals a new dimension to the relationship between translation activity and interactions between mRNA, the translation machinery and decapping activator proteins. ..
- Pittman Y, Kandl K, Lewis M, Valente L, Kinzy T. Coordination of eukaryotic translation elongation factor 1A (eEF1A) function in actin organization and translation elongation by the guanine nucleotide exchange factor eEF1Balpha. J Biol Chem. 2009;284:4739-47 pubmed publisher..The consequences of the overlapping functions in this eEF1A domain and its unique differences from the bacterial homologs provide a novel function for eEF1Balpha to balance the dual roles in actin bundling and protein synthesis. ..
- Mishra A, Gangwani L, Davis R, Lambright D. Structural insights into the interaction of the evolutionarily conserved ZPR1 domain tandem with eukaryotic EF1A, receptors, and SMN complexes. Proc Natl Acad Sci U S A. 2007;104:13930-5 pubmed..Structural differences between the ZPR1 domains contribute to the observed functional divergence and provide evidence for distinct modalities of interaction with eEF1A and survival motor neuron complexes. ..
- Aström S, Nordlund M, Erickson F, Hannig E, Byström A. Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiae. Mol Gen Genet. 1999;261:967-76 pubmed..On the other hand, introduction of a high-copy-number plasmid carrying the TEF2 gene, which encodes the eukaryotic elongation factor 1alpha (eEF-1alpha), into rit1 null strains with two intact ..
- Lipson R, Webb K, Clarke S. Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae. Arch Biochem Biophys. 2010;500:137-43 pubmed publisher..We suggest that YHL039W (now designated EFM1 for elongation factor methyltransferase 1) and YIL064W/SEE1 encode distinct eEF1A methyltransferases that respectively monomethylate and dimethylate this protein at lysine residues. ..
- Calvert M, Keck K, Ptak C, Shabanowitz J, Hunt D, Pemberton L. Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol. 2008;28:1313-25 pubmed..In conclusion, our data show that Nap1 phosphorylation by CK2 appears to regulate Nap1 localization and is required for normal progression through S phase. ..
- Naticchia M, Brown H, Garcia F, Lamade A, Justice S, Herrin R, et al. Bifunctional electrophiles cross-link thioredoxins with redox relay partners in cells. Chem Res Toxicol. 2013;26:490-7 pubmed publisher..Taken together, our results indicate that bifunctional electrophiles potentially disrupt redox homeostasis in yeast and human cells by forming cross-linked complexes between thioredoxins and their redox partners. ..
- Bodman J, Yang Y, Logan M, Eitzen G. Yeast translation elongation factor-1A binds vacuole-localized Rho1p to facilitate membrane integrity through F-actin remodeling. J Biol Chem. 2015;290:4705-16 pubmed publisher..Here, we identified eEF1A as a vacuolar Rho1p-interacting protein. eEF1A (encoded by the TEF1 and TEF2 genes in yeast) is an aminoacyl-tRNA transferase needed during protein translation...
- Kwapisz M, Cholbinski P, Hopper A, Rousset J, Zoladek T. Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae. RNA. 2005;11:1710-8 pubmed..This defect, also reversed by overproduction of the elongation factor eEF1A, may be the primary reason for altered translational decoding accuracy. ..
- Carr Schmid A, Durko N, Cavallius J, Merrick W, Kinzy T. Mutations in a GTP-binding motif of eukaryotic elongation factor 1A reduce both translational fidelity and the requirement for nucleotide exchange. J Biol Chem. 1999;274:30297-302 pubmed..Additionally, eEF1A mutations that suppress the requirement for guanine nucleotide exchange may not effectively perform all the functions of eEF1A in vivo. ..
- Krokowski D, Tchorzewski M, Boguszewska A, McKay A, Maslen S, Robinson C, et al. Elevated copy number of L-A virus in yeast mutant strains defective in ribosomal stalk. Biochem Biophys Res Commun. 2007;355:575-80 pubmed
- Waller T, Lee S, Sattlegger E. Evidence that Yih1 resides in a complex with ribosomes. FEBS J. 2012;279:1761-76 pubmed publisher..Close physical proximity of Yih1 to the Gcn1-Gcn2-ribosome complex would allow cells to quickly inhibit Gcn2 whenever or wherever necessary. ..
- Munshi R, Kandl K, Carr Schmid A, Whitacre J, Adams A, Kinzy T. Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast. Genetics. 2001;157:1425-36 pubmed..As the ability of eEF1A to bind and bundle actin is conserved in yeast, these results link the established ability of eEF1A to bind and bundle actin in vitro with nontranslational roles for the protein in vivo. ..
- Huang H, Hopper A. In vivo biochemical analyses reveal distinct roles of Î²-importins and eEF1A in tRNA subcellular traffic. Genes Dev. 2015;29:772-83 pubmed publisher..Assembly and/or stability of this quaternary complex requires Tef1/2, thereby facilitating efficient re-export of aminoacylated tRNAs to the cytoplasm. ..