Gene Symbol: TAT2
Description: aromatic amino acid transmembrane transporter TAT2
Alias: LTG3, SAB2, SCM2, TAP2, aromatic amino acid transmembrane transporter TAT2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Schmidt A, Hall M, Koller A. Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake. Mol Cell Biol. 1994;14:6597-606 pubmed
    ..cerevisiae is required for amino acid import and that overexpression of two new genes, TAT1 and TAT2 (formerly called TAP1 and TAP2), confers resistance to the drug...
  2. Umebayashi K, Nakano A. Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J Cell Biol. 2003;161:1117-31 pubmed
    ..The sorting mechanisms of Tat2p that depend on both sterol and ubiquitin will be discussed. ..
  3. Yoko o T, Ichikawa D, Miyagishi Y, Kato A, Umemura M, Takase K, et al. Determination and physiological roles of the glycosylphosphatidylinositol lipid remodelling pathway in yeast. Mol Microbiol. 2013;88:140-55 pubmed publisher
    ..In addition, mass spectrometric analysis of lipid species of Flag-tagged Gas1p revealed that Gas1p contains ceramide moieties in its GPI anchor. ..
  4. Miura T, Abe F. Multiple ubiquitin-specific protease genes are involved in degradation of yeast tryptophan permease Tat2 at high pressure. FEMS Microbiol Lett. 2004;239:171-9 pubmed
    When Saccharomyces cerevisiae cells are exposed to high hydrostatic pressure, tryptophan permease Tat2 is degraded in a manner dependent on Rsp5 ubiquitin ligase. Consequently, cell growth is arrested in tryptophan auxotrophic strains...
  5. Hachiro T, Yamamoto T, Nakano K, Tanaka K. Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast. J Biol Chem. 2013;288:3594-608 pubmed publisher
  6. Johzuka K, Horiuchi T. The cis element and factors required for condensin recruitment to chromosomes. Mol Cell. 2009;34:26-35 pubmed publisher
    ..Our study also suggests that recruitment of condensin onto chromosomes requires cis elements and recruiters that physically interact with condensin. ..
  7. Shin Y, Goo D, So I, Rhode P, Campbell J, Kim J. Isolation and characterization of Saccharomyces cerevisiae SAB2, a suppressor gene for temperature-sensitive phenotype of ARS-binding factor 1 mutant. Biochem Mol Biol Int. 1996;40:915-21 pubmed
    A high-copy number suppressor of yeast abf1-5 mutant, a temperature-sensitive lethal mutant, was isolated and named SAB2 (suppressor of ABF1)...
  8. Nakamura H, Miura K, Fukuda Y, Shibuya I, Ohta A, Takagi M. Phosphatidylserine synthesis required for the maximal tryptophan transport activity in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2000;64:167-72 pubmed
    ..was restored either by expression of CHO1/PSS or by introduction of a gene encoding tryptophan transporter, TAT1 or TAT2. These results indicate that PS synthesis is required for the maximal tryptophan-transporting activity of S...
  9. Khozoie C, Pleass R, Avery S. The antimalarial drug quinine disrupts Tat2p-mediated tryptophan transport and causes tryptophan starvation. J Biol Chem. 2009;284:17968-74 pubmed publisher
    ..quinine sensitivity of a trp1Delta mutant could be rescued by overexpression of Trp permeases, encoded by TAT1 and TAT2. The site of quinine action was identified specifically as the high affinity Trp/Tyr permease, Tat2p, with which ..

More Information


  1. Johnson S, Hanson P, Manoharlal R, Brice S, Cowart L, Moye Rowley W. Regulation of yeast nutrient permease endocytosis by ATP-binding cassette transporters and a seven-transmembrane protein, RSB1. J Biol Chem. 2010;285:35792-802 pubmed publisher
    ..of yeast because this bioactive ceramide precursor causes diversion of the high affinity tryptophan permease Tat2 to the vacuole rather than the plasma membrane...
  2. Popov Celeketic D, Bianchi F, Ruiz S, Meutiawati F, Poolman B. A Plasma Membrane Association Module in Yeast Amino Acid Transporters. J Biol Chem. 2016;291:16024-37 pubmed publisher
    ..Using in silico analyses and mutational studies we found that the C-terminal sequences of Gap1, Bap2, Hip1, Tat1, Tat2, Mmp1, Sam3, Agp1, and Gnp1 are about 50 residues long, associate with the PM, and have features that discriminate ..
  3. Tanaka N, Mukai Y. Yeast Cyc8p and Tup1p proteins function as coactivators for transcription of Stp1/2p-dependent amino acid transporter genes. Biochem Biophys Res Commun. 2015;468:32-8 pubmed publisher
    ..Herein, we found tryptophan transporter genes, TAT1 and TAT2, that, when overexpressed, suppressed the slow growth of Δcyc8...
  4. Kanda N, Abe F. Structural and functional implications of the yeast high-affinity tryptophan permease Tat2. Biochemistry. 2013;52:4296-307 pubmed publisher
    ..study in Saccharomyces cerevisiae demonstrated that tryptophan import by the high-affinity tryptophan permease Tat2 is accompanied by a large volume increase during substrate import...
  5. Okamoto M, Yoko o T, Umemura M, Nakayama K, Jigami Y. Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins Tat2p and Fur4p. J Biol Chem. 2006;281:4013-23 pubmed
    ..We identified TAT2, which encodes a high affinity tryptophan permease, as a multicopy suppressor of cold sensitivity in gwt1-10 cells...
  6. López Mirabal H, Winther J, Kielland Brandt M. Oxidant resistance in a yeast mutant deficient in the Sit4 phosphatase. Curr Genet. 2008;53:275-86 pubmed publisher
    ..A relation between actin homeostasis and DPS resistance of sit4-110 cannot be ruled out, but our results suggest that unknown pathways might be involved in DPS resistance through mechanisms involving the Sit4p and/or Tap42p function(s). ..
  7. Ballester Tomás L, Prieto J, Alepuz P, González A, Garre E, Randez Gil F. Inappropriate translation inhibition and P-body formation cause cold-sensitivity in tryptophan-auxotroph yeast mutants. Biochim Biophys Acta Mol Cell Res. 2017;1864:314-323 pubmed publisher
    ..Finally, we demonstrated that P-body formation responds to a downshift in temperature in a TRP1-dependent manner and is required for cold tolerance. ..
  8. Hiraki T, Abe F. Overexpression of Sna3 stabilizes tryptophan permease Tat2, potentially competing for the WW domain of Rsp5 ubiquitin ligase with its binding protein Bul1. FEBS Lett. 2010;584:55-60 pubmed publisher
    Tryptophan permease Tat2 in Saccharomyces cerevisiae undergoes Rsp5-dependent degradation upon exposure to high hydrostatic pressure and it limits the growth of tryptophan auxotrophs...
  9. Daicho K, Makino N, Hiraki T, Ueno M, Uritani M, Abe F, et al. Sorting defects of the tryptophan permease Tat2 in an erg2 yeast mutant. FEMS Microbiol Lett. 2009;298:218-27 pubmed publisher
    ..but not that of Erg6 involved in a late step, is compromised, vacuolar degradation of the tryptophan permease Tat2 is promoted...
  10. Liu M, Brusilow W, Needleman R. Activity of the yeast Tat2p tryptophan permease is sensitive to the anti-tumor agent 4-phenylbutyrate. Curr Genet. 2004;46:256-68 pubmed
  11. Nagayama A, Kato C, Abe F. The N- and C-terminal mutations in tryptophan permease Tat2 confer cell growth in Saccharomyces cerevisiae under high-pressure and low-temperature conditions. Extremophiles. 2004;8:143-9 pubmed
    ..When the cells are subjected to a pressure of 25 MPa, tryptophan permease Tat2 is degraded in a manner dependent on ubiquitination by Rsp5...
  12. Regenberg B, Düring Olsen L, Kielland Brandt M, Holmberg S. Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae. Curr Genet. 1999;36:317-28 pubmed
    ..These findings modify the prevalent view that S. cerevisiae only contains one general amino-acid permease, Gap1p, and a number of permeases that are specific for a single or a few amino acids. ..
  13. Chen X, Xiao Z, Fitzgerald Hayes M. SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth. Mol Gen Genet. 1994;244:260-8 pubmed
    b>SCM2, a novel gene encoding a yeast tryptophan permease, was cloned as a high-copy-number suppressor of cse2-1. The cse2-1 mutation causes cold sensitivity, temperature sensitivity and chromosome missegregation...
  14. Abe F, Iida H. Pressure-induced differential regulation of the two tryptophan permeases Tat1 and Tat2 by ubiquitin ligase Rsp5 and its binding proteins, Bul1 and Bul2. Mol Cell Biol. 2003;23:7566-84 pubmed
    ..Here we present evidence that the two tryptophan permeases Tat1 and Tat2 are differentially regulated by Rsp5 ubiquitin ligase in response to high hydrostatic pressure...
  15. Bauer B, Rossington D, Mollapour M, Mamnun Y, Kuchler K, Piper P. Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong influence of amino acid auxotrophies on the phenotypes of membrane transporter mutants. Eur J Biochem. 2003;270:3189-95 pubmed
    ..These transporters do not confer resistance to high acetate levels and, in prototrophs, their presence is actually detrimental for this resistance...