Gene Symbol: TAP42
Description: Tap42p
Alias: Tap42p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Yan G, Shen X, Jiang Y. Rapamycin activates Tap42-associated phosphatases by abrogating their association with Tor complex 1. EMBO J. 2006;25:3546-55 pubmed
    In Saccharomyces cerevisiae, the Tap42-phosphatase complexes are major targets of the Tor kinases in the rapamycin-sensitive signaling pathway...
  2. Wang H, Wang X, Jiang Y. Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases. Mol Biol Cell. 2003;14:4342-51 pubmed
    ..for Pph21/22 and the Sap proteins for Sit4, these phosphatases have been found to associate with a protein termed Tap42. In this study, we demonstrated that Sit4 and PP2Ac interact with Tap42 via an N-terminal domain that is conserved ..
  3. Di Como C, Arndt K. Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases. Genes Dev. 1996;10:1904-16 pubmed
    We identified an essential Saccharomyces cerevisiae protein, Tap42, that associates with Sit4, a type 2A-related protein phosphatase, and with the type 2A phosphatase catalytic subunits...
  4. Wu J, Tolstykh T, Lee J, Boyd K, Stock J, Broach J. Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo. EMBO J. 2000;19:5672-81 pubmed
    ..These phenotypes can be reversed by overexpression of the B regulatory subunit. These results demonstrate that Ppm1 is the sole PP2A methyltransferase in yeast and that its activity is required for the integrity of the PP2A holoenzyme. ..
  5. Van Hoof C, Martens E, Longin S, Jordens J, Stevens I, Janssens V, et al. Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2. Biochem J. 2005;386:93-102 pubmed
    ..The Ypa1 and Ypa2 proteins do not compete with Tap42 (PP2A associating protein) for binding to PP2A family members...
  6. Santhanam A, Hartley A, Düvel K, Broach J, Garrett S. PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p. Eukaryot Cell. 2004;3:1261-71 pubmed
    ..Inactivation of the Tor pathway component Tap42p using tap42(Ts) alleles causes a sustained nuclear localization similar to that after the addition of the Tor ..
  7. Jiang Y, Broach J. Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast. EMBO J. 1999;18:2782-92 pubmed
    ..This leads to diminished association of a protein, Tap42, with two different protein phosphatase catalytic subunits; one encoded redundantly by PPH21 and PPH22, and one ..
  8. Hombauer H, Weismann D, Mudrak I, Stanzel C, Fellner T, Lackner D, et al. Generation of active protein phosphatase 2A is coupled to holoenzyme assembly. PLoS Biol. 2007;5:e155 pubmed
    ..We propose a novel model of PP2A biogenesis in which a tightly controlled activation cascade protects cells from untargeted activity of the free catalytic PP2A subunit. ..
  9. Zheng Y, Jiang Y. The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes. Mol Biol Cell. 2005;16:2119-27 pubmed
    ..We show that the yeast PTPA is an integral part of the Tap42-phosphatase complexes that act downstream of the Tor proteins, the target of rapamycin...

More Information


  1. Wang H, Jiang Y. The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeast. Mol Cell Biol. 2003;23:3116-25 pubmed
    ..Tor-mediated signaling activity promotes the interaction of phosphatase-interacting protein Tap42 with PP2A and 2A-like protein phosphatases...
  2. Jacinto E, Guo B, Arndt K, Schmelzle T, Hall M. TIP41 interacts with TAP42 and negatively regulates the TOR signaling pathway. Mol Cell. 2001;8:1017-26 pubmed
    ..regulate the type 2A-related phosphatase SIT4 by promoting the association of this phosphatase with the inhibitor TAP42. Here, we describe TIP41, a conserved TAP42-interacting protein involved in the regulation of SIT4...
  3. Kuepfer L, Peter M, Sauer U, Stelling J. Ensemble modeling for analysis of cell signaling dynamics. Nat Biotechnol. 2007;25:1001-6 pubmed
    ..PP2As), our integrated computational and experimental analysis proposes a specificity factor, based on Tap42p-Tip41p, for PP2As as the key signaling mechanism that is quantitatively consistent with all available experimental ..
  4. López Mirabal H, Winther J, Kielland Brandt M. Oxidant resistance in a yeast mutant deficient in the Sit4 phosphatase. Curr Genet. 2008;53:275-86 pubmed publisher
    ..only Tip41p is involved in DPS-resistance; both Delta tip41 deletion and overexpression of the Tip41p target Tap42p resulted in increased DPS-resistance...
  5. Bruckner S, Kern S, Birke R, Saugar I, Ulrich H, Mösch H. The TEA transcription factor Tec1 links TOR and MAPK pathways to coordinate yeast development. Genetics. 2011;189:479-94 pubmed publisher
    ..is under control of the nutrient-sensitive target of rapamycin complex 1 (TORC1) signaling pathway via the Tip41-Tap42-Sit4 branch...
  6. Yorimitsu T, He C, Wang K, Klionsky D. Tap42-associated protein phosphatase type 2A negatively regulates induction of autophagy. Autophagy. 2009;5:616-24 pubmed
    ..Here, we find that Tap42 and protein phosphatase type 2A (PP2A) are involved in the regulation of autophagy in yeast...
  7. Huber A, Bodenmiller B, Uotila A, Stahl M, Wanka S, Gerrits B, et al. Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis. Genes Dev. 2009;23:1929-43 pubmed publisher
    ..Although two direct effectors of yeast TORC1 have been reported (Tap42, a regulator of PP2A phosphatases and Sch9, an AGC family kinase), the signaling pathways that couple TORC1 to its ..
  8. Garipler G, Mutlu N, Lack N, Dunn C. Deletion of conserved protein phosphatases reverses defects associated with mitochondrial DNA damage in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2014;111:1473-8 pubmed publisher
    ..We have examined the role of phosphatases under the control of the conserved ?4/Tap42 protein in cells lacking a mitochondrial genome...
  9. González A, Ruiz A, Casamayor A, Arino J. Normal function of the yeast TOR pathway requires the type 2C protein phosphatase Ptc1. Mol Cell Biol. 2009;29:2876-88 pubmed publisher
    ..Epitasis analysis indicates that the mutation of SIT4 or TIP41, encoding a Tap42-interacting protein, abolishes the sensitivity of the ptc1 strain to rapamycin and caffeine...
  10. Jiang Y. An essential role of Tap42-associated PP2A and 2A-like phosphatases in Ty1 transcriptional silencing of S. cerevisiae. Yeast. 2008;25:755-64 pubmed publisher
    ..The role of Tap42 (a key downstream component of the TORC1 pathway) was tested...
  11. Jablonowski D, Täubert J, Bär C, Stark M, Schaffrath R. Distinct subsets of Sit4 holophosphatases are required for inhibition of Saccharomyces cerevisiae growth by rapamycin and zymocin. Eukaryot Cell. 2009;8:1637-47 pubmed publisher
    ..Here, we show that the rapamycin effector Tap42, which interacts with Sit4, is dispensable for zymocin action...
  12. Yan G, Lai Y, Jiang Y. The TOR complex 1 is a direct target of Rho1 GTPase. Mol Cell. 2012;45:743-53 pubmed publisher
    ..The binding also triggers the release and activation of the Tap42-2A phosphatase, a major effector of TORC1 that resides on the complex...
  13. Juanes M, Khoueiry R, Kupka T, Castro A, Mudrak I, Ogris E, et al. Budding yeast greatwall and endosulfines control activity and spatial regulation of PP2A(Cdc55) for timely mitotic progression. PLoS Genet. 2013;9:e1003575 pubmed publisher
    ..However, this conserved module is adapted to account for differences in the regulation of mitotic entry in different organisms. ..
  14. Yang J, Roe S, Prickett T, Brautigan D, Barford D. The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation. Biochemistry. 2007;46:8807-15 pubmed
    ..A distinct population of PP2Ac associates with the Tap42/alpha4 subunit, an interaction mutually exclusive with that of PR65/A...