Gene Symbol: TAF12
Description: Taf12p
Alias: TAF61, TAF68, Taf12p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Köhler A, Schneider M, Cabal G, Nehrbass U, Hurt E. Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export. Nat Cell Biol. 2008;10:707-15 pubmed publisher
    ..Thus, Sgf73 provides a molecular scaffold to integrate the regulation of H2B ubiquitin levels, tethering of a gene to the NPC and export of mRNA. ..
  2. Lee K, Florens L, Swanson S, Washburn M, Workman J. The deubiquitylation activity of Ubp8 is dependent upon Sgf11 and its association with the SAGA complex. Mol Cell Biol. 2005;25:1173-82 pubmed
    ..Taken together, these data indicate that the expression of some genes, including ARG1, is regulated by a balance of histone H2B ubiquitylation in the cell. ..
  3. Daniel J, Torok M, Sun Z, Schieltz D, Allis C, Yates J, et al. Deubiquitination of histone H2B by a yeast acetyltransferase complex regulates transcription. J Biol Chem. 2004;279:1867-71 pubmed
    ..Collectively, these data suggest that the SAGA and SLIK HAT complexes can regulate an integrated set of multiple histone modifications, counteracting repressive effects that alter chromatin and regulate gene expression. ..
  4. Pascual García P, Govind C, Queralt E, Cuenca Bono B, Llopis A, Chávez S, et al. Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2. Genes Dev. 2008;22:2811-22 pubmed publisher
    ..Our results reveal that Sus1 plays a key role in coordinating gene transcription and mRNA export by working at the interface between the SAGA and TREX2 complexes during transcription elongation. ..
  5. Wu P, Ruhlmann C, Winston F, Schultz P. Molecular architecture of the S. cerevisiae SAGA complex. Mol Cell. 2004;15:199-208 pubmed
    ..Our data provide insights into the molecular architecture of SAGA and imply a functional organization to the complex. ..
  6. Wu P, Winston F. Analysis of Spt7 function in the Saccharomyces cerevisiae SAGA coactivator complex. Mol Cell Biol. 2002;22:5367-79 pubmed
    ..Analysis of an spt7 mutant with greatly reduced levels of SLIK/SAGA(alt)/SALSA suggests a subtle role for this complex in transcription that may be redundant with a subset of SAGA functions. ..
  7. Lee K, Swanson S, Florens L, Washburn M, Workman J. Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes. Epigenetics Chromatin. 2009;2:2 pubmed publisher
  8. Bhaumik S, Green M. SAGA is an essential in vivo target of the yeast acidic activator Gal4p. Genes Dev. 2001;15:1935-45 pubmed
    ..Based on these and other results, we conclude that SAGA is an essential target of Gal4p that, following recruitment to the UAS, facilitates PIC assembly and transcription. ..
  9. Köhler A, Pascual García P, Llopis A, Zapater M, Posas F, Hurt E, et al. The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11. Mol Biol Cell. 2006;17:4228-36 pubmed
    ..Interestingly, sgf11 deletion enhances the mRNA export defect observed in sus1delta cells. Thus, the Sus1-Sgf11-Ubp8 module could work at the junction between SAGA-dependent transcription and nuclear mRNA export. ..

More Information


  1. Powell D, Weaver C, Jennings J, McAfee K, He Y, Weil P, et al. Cluster analysis of mass spectrometry data reveals a novel component of SAGA. Mol Cell Biol. 2004;24:7249-59 pubmed
    ..Our data suggest that the role of SGF11 in transcription is independent of SAGA's histone acetyltransferase activity but may involve Ubp8p recruitment to or stabilization in SAGA. ..
  2. Reese J, Zhang Z, Kurpad H. Identification of a yeast transcription factor IID subunit, TSG2/TAF48. J Biol Chem. 2000;275:17391-8 pubmed
    ..We have identified a high copy suppressor of the TAF68/61 mutation, TSG2, that has sequence similarity to a region of the SAGA subunit Ada1...
  3. García Oliver E, Pascual García P, García Molinero V, Lenstra T, Holstege F, Rodriguez Navarro S. A novel role for Sem1 and TREX-2 in transcription involves their impact on recruitment and H2B deubiquitylation activity of SAGA. Nucleic Acids Res. 2013;41:5655-68 pubmed publisher
    ..These results unveil a new role for Sem1 in the activation of the SAGA-dependent gene GAL1 and influencing H2B deubiquitylation. Our work provides insights into a novel functional relationship between Sem1 and the SAGA complex. ..
  4. Larschan E, Winston F. The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4. Genes Dev. 2001;15:1946-56 pubmed
    ..These results, taken together with previous studies, demonstrate a dependent pathway for the recruitment of TBP to GAL gene promoters consisting of the recruitment of SAGA by Gal4 and the subsequent recruitment of TBP by SAGA. ..
  5. Boyer L, Langer M, Crowley K, Tan S, Denu J, Peterson C. Essential role for the SANT domain in the functioning of multiple chromatin remodeling enzymes. Mol Cell. 2002;10:935-42 pubmed
    ..Our results are consistent with a general role for SANT domains in functional interactions with histone N-terminal tails. ..
  6. Sterner D, Wang X, Bloom M, Simon G, Berger S. The SANT domain of Ada2 is required for normal acetylation of histones by the yeast SAGA complex. J Biol Chem. 2002;277:8178-86 pubmed
    ..These results suggest that histone and nucleosomal substrate recognition by SAGA involves multiple conserved domains and proteins, beyond those that have been previously identified. ..
  7. Pray Grant M, Daniel J, Schieltz D, Yates J, Grant P. Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation. Nature. 2005;433:434-8 pubmed
    ..Our study identifies the first chromodomain that recognizes methylated histone H3 (Lys 4) and possibly identifies a larger subfamily of chromodomain proteins with similar recognition properties. ..
  8. Kirchner J, Sanders S, Klebanow E, Weil P. Molecular genetic dissection of TAF25, an essential yeast gene encoding a subunit shared by TFIID and SAGA multiprotein transcription factors. Mol Cell Biol. 2001;21:6668-80 pubmed
    ..The results of these analyses show that TAF25p is comprised of multiple mutable elements which contribute importantly to RNA polymerase II-mediated mRNA gene transcription. ..
  9. Romier C, James N, Birck C, Cavarelli J, Vivares C, Collart M, et al. Crystal structure, biochemical and genetic characterization of yeast and E. cuniculi TAF(II)5 N-terminal domain: implications for TFIID assembly. J Mol Biol. 2007;368:1292-306 pubmed
  10. Shen W, Bhaumik S, Causton H, Simon I, Zhu X, Jennings E, et al. Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly. EMBO J. 2003;22:3395-402 pubmed
    ..Collectively, our results confirm and extend the proposal that individual TAFs have selective transcriptional roles and distinct functions. ..
  11. Reese J, Green M. Genetic analysis of TAF68/61 reveals links to cell cycle regulators. Yeast. 2001;18:1197-205 pubmed
    ..In this report, a temperature-sensitive mutant of TAF68/61 was used to screen for high-copy dosage suppressors of its growth defect...
  12. Setiaputra D, Ross J, Lu S, Cheng D, Dong M, Yip C. Conformational flexibility and subunit arrangement of the modular yeast Spt-Ada-Gcn5 acetyltransferase complex. J Biol Chem. 2015;290:10057-70 pubmed publisher
    ..Our results relate information of overall SAGA structure with detailed subunit level interactions, improving our understanding of its architecture and flexibility. ..
  13. Matangkasombut O, Buratowski R, Swilling N, Buratowski S. Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev. 2000;14:951-62 pubmed
    ..The structural and functional similarities suggest that Bdf1 corresponds to the carboxy-terminal region of higher eukaryotic TAF(II)250 and that the interaction between TFIID and Bdf1 is important for proper gene expression. ..
  14. Sharma V, Li B, Reese J. SWI/SNF-dependent chromatin remodeling of RNR3 requires TAF(II)s and the general transcription machinery. Genes Dev. 2003;17:502-15 pubmed
    ..Thus, the general transcription machinery can contribute to nucleosome remodeling by mediating the association of SWI/SNF with promoters, thereby revealing a novel pathway for the recruitment of chromatin remodeling activities. ..
  15. Tomar R, Zheng S, Brunke Reese D, Wolcott H, Reese J. Yeast Rap1 contributes to genomic integrity by activating DNA damage repair genes. EMBO J. 2008;27:1575-84 pubmed publisher
    ..We propose that Rap1 acts as a rheostat controlling nucleotide pools in response to shortened telomeres and DNA damage, providing a mechanism for fine-tuning the RNR genes during checkpoint activation. ..
  16. Li S, Shogren Knaak M. The Gcn5 bromodomain of the SAGA complex facilitates cooperative and cross-tail acetylation of nucleosomes. J Biol Chem. 2009;284:9411-7 pubmed publisher
  17. Leurent C, Sanders S, Ruhlmann C, Mallouh V, Weil P, Kirschner D, et al. Mapping histone fold TAFs within yeast TFIID. EMBO J. 2002;21:3424-33 pubmed
    ..Most of the HFD-containing TAFs have been found in two distinct lobes, thus revealing an unexpected and novel molecular organization of TFIID. ..
  18. Han Y, Luo J, Ranish J, Hahn S. Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex. EMBO J. 2014;33:2534-46 pubmed publisher
    ..Our results provide new insight into SAGA function in gene regulation, its structural similarity with TFIID, and functional interactions between the SAGA modules. ..
  19. Michel B, Komarnitsky P, Buratowski S. Histone-like TAFs are essential for transcription in vivo. Mol Cell. 1998;2:663-73 pubmed
    In yeast, the TBP-associated factors (TAFs) Taf17, Taf60, and Taf61(68) resemble histones H3, H4, and H2B, respectively. To analyze their roles in vivo, conditional alleles were isolated by mutagenizing their histone homology domains...
  20. Klein J, Nolden M, Sanders S, Kirchner J, Weil P, Melcher K. Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA. J Biol Chem. 2003;278:6779-86 pubmed
    ..Both ADs directly cross-linked to TBP and to a subset of TFIID and SAGA subunits that carry histone-fold motifs. ..
  21. Hall D, Struhl K. The VP16 activation domain interacts with multiple transcriptional components as determined by protein-protein cross-linking in vivo. J Biol Chem. 2002;277:46043-50 pubmed
    ..We show that the VP16 activation domain directly interacts with TATA-binding protein (TBP), TFIIB, and the SAGA histone acetylase complex in vivo. ..
  22. Stepanchick A, Zhi H, Cavanaugh A, Rothblum K, Schneider D, Rothblum L. DNA binding by the ribosomal DNA transcription factor rrn3 is essential for ribosomal DNA transcription. J Biol Chem. 2013;288:9135-44 pubmed publisher
    ..Moreover, although wild-type human Rrn3 complemented a yeast rrn3-ts mutant, the DNA-binding site mutant did not. These results demonstrate that DNA binding by Rrn3 is essential for transcription by RNA polymerase I. ..
  23. Spedale G, Mischerikow N, Heck A, Timmers H, Pijnappel W. Identification of Pep4p as the protease responsible for formation of the SAGA-related SLIK protein complex. J Biol Chem. 2010;285:22793-9 pubmed publisher
    ..Strains mimicking constitutive SLIK formation showed increased resistance to rapamycin treatment, suggesting a role for SLIK in regulating cellular responses to nutrient stress. ..
  24. Knutson B, Hahn S. Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexes. Mol Cell Biol. 2011;31:818-31 pubmed publisher
    ..Our results show that Tra1 recruitment at Gcn4-dependent and Rap1-dependent promoters requires the same regions of Tra1 and that separate regions of Tra1 contribute to the HAT activity and stability of the SAGA and NuA4 HAT modules. ..
  25. Auty R, Steen H, Myers L, Persinger J, Bartholomew B, Gygi S, et al. Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem. 2004;279:49973-81 pubmed
    ..TFIID supports basal transcription and activated transcription, both of which are enhanced by TFIIA. ..
  26. Brzovic P, Heikaus C, Kisselev L, Vernon R, Herbig E, Pacheco D, et al. The acidic transcription activator Gcn4 binds the mediator subunit Gal11/Med15 using a simple protein interface forming a fuzzy complex. Mol Cell. 2011;44:942-53 pubmed publisher
    ..Functional studies in yeast show the importance of residues at the protein interface, define the minimal requirements for a functional activator, and suggest a mechanism by which activators bind to multiple unrelated targets. ..
  27. Garbett K, Tripathi M, Cencki B, Layer J, Weil P. Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction. Mol Cell Biol. 2007;27:297-311 pubmed
    ..We conclude that Rap1p and TFIID directly interact and that this interaction contributes importantly to RP gene transcription. ..
  28. Li X, Bhaumik S, Green M. Distinct classes of yeast promoters revealed by differential TAF recruitment. Science. 2000;288:1242-4 pubmed
  29. Moqtaderi Z, Yale J, Struhl K, Buratowski S. Yeast homologues of higher eukaryotic TFIID subunits. Proc Natl Acad Sci U S A. 1996;93:14654-8 pubmed
    ..Based on their predicted molecular weights, these genes have been designated TAF67, TAF61(68), TAF40, TAF23(25), TAF19(FUN81), and TAF17...
  30. Belotserkovskaya R, Sterner D, Deng M, Sayre M, Lieberman P, Berger S. Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promoters. Mol Cell Biol. 2000;20:634-47 pubmed
    ..These results suggest that the composition of SAGA may be dynamic in vivo and may be regulated through dissociable inhibitory subunits. ..
  31. Sanders S, Weil P. Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex. J Biol Chem. 2000;275:13895-900 pubmed
    ..TAF complexes. The significance of these results in terms of TFIID structure, function, and organization is discussed. ..
  32. Koehler C, Bonnet J, Stierlé M, Romier C, Devys D, Kieffer B. DNA binding by Sgf11 protein affects histone H2B deubiquitination by Spt-Ada-Gcn5-acetyltransferase (SAGA). J Biol Chem. 2014;289:8989-99 pubmed publisher
    ..The combined interpretation of our results leads to an uncommon ZnF-DNA interaction between the SAGA DUBm and nucleosomes, thus providing further functional insights into SAGA's epigenetic modulation of the chromatin structure. ..
  33. Durand A, Bonnet J, Fournier M, Chavant V, Schultz P. Mapping the deubiquitination module within the SAGA complex. Structure. 2014;22:1553-9 pubmed
    ..A flexible protein arm brings both subunits close enough to interact simultaneously with TBP. ..
  34. Yatherajam G, Zhang L, Kraemer S, Stargell L. Protein-protein interaction map for yeast TFIID. Nucleic Acids Res. 2003;31:1252-60 pubmed
    ..Thus, these results serve as a foundation for understanding the overall architecture of yeast TFIID. ..
  35. Thuault S, Gangloff Y, Kirchner J, Sanders S, Werten S, Romier C, et al. Functional analysis of the TFIID-specific yeast TAF4 (yTAF(II)48) reveals an unexpected organization of its histone-fold domain. J Biol Chem. 2002;277:45510-7 pubmed
    ..These results reveal an unexpected and novel HFD organization in which the alpha3 helix is separated from the alpha2 helix by an extended loop containing a conserved functional domain. ..
  36. Papai G, Tripathi M, Ruhlmann C, Werten S, Crucifix C, Weil P, et al. Mapping the initiator binding Taf2 subunit in the structure of hydrated yeast TFIID. Structure. 2009;17:363-73 pubmed publisher
    ..Mapping the position of this crucial subunit in the vicinity of Taf1p and of TBP sheds new light on its role in promoter recognition. ..
  37. Mencia M, Struhl K. Region of yeast TAF 130 required for TFIID to associate with promoters. Mol Cell Biol. 2001;21:1145-54 pubmed
    ..These results suggest that the C-terminal region of TAF130 is required for TFIID to associate with promoters. ..
  38. Qiu H, Hu C, Zhang F, Hwang G, Swanson M, Boonchird C, et al. Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4p. Mol Cell Biol. 2005;25:3461-74 pubmed
    ..Thus, while Tra1p can bind directly to Gcn4p in vitro, it requires other SAGA subunits for efficient recruitment in vivo. ..
  39. Grant P, Schieltz D, Pray Grant M, Steger D, Reese J, Yates J, et al. A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation. Cell. 1998;94:45-53 pubmed
    ..These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145. ..
  40. Natarajan K, Jackson B, Rhee E, Hinnebusch A. yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex. Mol Cell. 1998;2:683-92 pubmed
    ..The taf61-1 mutation impairs binding of Gcn4p to SAGA/yTAFII subunits but not to components of holoenzyme mediator...
  41. Pray Grant M, Schieltz D, McMahon S, Wood J, Kennedy E, Cook R, et al. The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway. Mol Cell Biol. 2002;22:8774-86 pubmed
  42. Sanders S, Garbett K, Weil P. Molecular characterization of Saccharomyces cerevisiae TFIID. Mol Cell Biol. 2002;22:6000-13 pubmed
    ..The results of direct biochemical exchange experiments confirmed this hypothesis. Together, our results represent a concise molecular characterization of the general transcription factor TFIID from S. cerevisiae. ..
  43. Durso R, Fisher A, Albright Frey T, Reese J. Analysis of TAF90 mutants displaying allele-specific and broad defects in transcription. Mol Cell Biol. 2001;21:7331-44 pubmed
  44. Kirschner D, vom Baur E, Thibault C, Sanders S, Gangloff Y, Davidson I, et al. Distinct mutations in yeast TAF(II)25 differentially affect the composition of TFIID and SAGA complexes as well as global gene expression patterns. Mol Cell Biol. 2002;22:3178-93 pubmed
    ..Thus, different yTAF(II)25 mutations induce distinct phenotypes and affect the regulation of different subsets of genes, demonstrating that no individual TAF(II) mutant allele reflects the full range of its normal functions. ..
  45. Badarinarayana V, Chiang Y, Denis C. Functional interaction of CCR4-NOT proteins with TATAA-binding protein (TBP) and its associated factors in yeast. Genetics. 2000;155:1045-54 pubmed
    ..These genetic and physical interactions indicate that one role of the CCR4-NOT proteins is to inhibit functional TBP-DNA interactions, perhaps by interacting with and modulating the function of TFIID. ..
  46. Kamata K, Hatanaka A, Goswami G, Shinmyozu K, Nakayama J, Urano T, et al. C-terminus of the Sgf73 subunit of SAGA and SLIK is important for retention in the larger complex and for heterochromatin boundary function. Genes Cells. 2013;18:823-37 pubmed publisher
    ..Western blot analysis detected both the full-length and truncated forms of Spt7, suggesting that SAGA and SLIK complex formation is important for the boundary function of Sgf73. ..
  47. Kobayashi A, Miyake T, Kawaichi M, Kokubo T. Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP). Nucleic Acids Res. 2003;31:1261-74 pubmed
    ..another nsl mutation in the same screen and identified it to be a mutation in the histone fold domain (HFD) of the TAF12 gene...
  48. Herbig E, Warfield L, Fish L, Fishburn J, Knutson B, Moorefield B, et al. Mechanism of Mediator recruitment by tandem Gcn4 activation domains and three Gal11 activator-binding domains. Mol Cell Biol. 2010;30:2376-90 pubmed publisher
    ..The individual Gcn4 activation domains cross-link to three common targets, Gal11/Med15, Taf12, and Tra1, which are subunits of four conserved coactivator complexes, Mediator, SAGA, TFIID, and NuA4...
  49. Sterner D, Grant P, Roberts S, Duggan L, Belotserkovskaya R, Pacella L, et al. Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol. 1999;19:86-98 pubmed
    ..Loss of either of these causes slight impairment in vivo, but loss of both is highly detrimental to growth and transcription. ..
  50. Selleck W, Howley R, Fang Q, Podolny V, Fried M, Buratowski S, et al. A histone fold TAF octamer within the yeast TFIID transcriptional coactivator. Nat Struct Biol. 2001;8:695-700 pubmed
    ..Our results indicate that the TAF octamer is similar both in stoichiometry and histone fold interactions to the histone octamer component of chromatin. ..
  51. Gangloff Y, Werten S, Romier C, Carre L, Poch O, Moras D, et al. The human TFIID components TAF(II)135 and TAF(II)20 and the yeast SAGA components ADA1 and TAF(II)68 heterodimerize to form histone-like pairs. Mol Cell Biol. 2000;20:340-51 pubmed
    ..These results are indicative of a histone fold type of interaction between hTAF(II)20-hTAF(II)135 and yTAF(II)68-yADA1, which therefore constitute novel histone-like pairs in the TFIID and SAGA complexes. ..
  52. Hintze S, Engelhardt M, van Diepen L, Witt E, Schüller H. Multiple Taf subunits of TFIID interact with Ino2 activation domains and contribute to expression of genes required for yeast phospholipid biosynthesis. Mol Microbiol. 2017;: pubmed publisher
    ..that multiple subunits of basal transcription factor TFIID (TBP-associated factors Taf1, Taf4, Taf6, Taf10 and Taf12) are able to interact in vitro with activation domains of Ino2...
  53. Saint M, Sawhney S, Sinha I, Singh R, Dahiya R, Thakur A, et al. The TAF9 C-terminal conserved region domain is required for SAGA and TFIID promoter occupancy to promote transcriptional activation. Mol Cell Biol. 2014;34:1547-63 pubmed publisher
    ..These results suggest a crucial role for the Taf9 CRD in genome-wide transcription and highlight the importance of conserved domains, other than histone fold domains, as a common determinant for TFIID and SAGA functions. ..
  54. Bian C, Xu C, Ruan J, Lee K, Burke T, Tempel W, et al. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation. EMBO J. 2011;30:2829-42 pubmed publisher
    ..Our in vitro and in vivo functional assays show that Sgf29 recognizes methylated H3K4 to recruit the SAGA complex to its targets sites and mediates histone H3 acetylation, underscoring the importance of Sgf29 in gene regulation. ..
  55. Grant P, Schieltz D, Pray Grant M, Yates J, Workman J. The ATM-related cofactor Tra1 is a component of the purified SAGA complex. Mol Cell. 1998;2:863-7 pubmed
    ..These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter. ..
  56. Cavanaugh A, Hirschler Laszkiewicz I, Hu Q, Dundr M, Smink T, Misteli T, et al. Rrn3 phosphorylation is a regulatory checkpoint for ribosome biogenesis. J Biol Chem. 2002;277:27423-32 pubmed
    ..These results suggest that the phosphorylation state of Rrn3 regulates rDNA transcription by determining the steady-state concentration of the Rrn3.RNA polymerase I complex within the nucleolus. ..
  57. Takahata S, Kasahara K, Kawaichi M, Kokubo T. Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulation. Mol Cell Biol. 2004;24:3089-99 pubmed
    ..These results indicate that there is no or minimal geometric constraint on the ability of the TAND to function normally in transcriptional regulation as long as TFIID assembly is secured. ..
  58. Sermwittayawong D, Tan S. SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment. EMBO J. 2006;25:3791-800 pubmed
    ..This simple model can explain SAGA's observed ability to both activate and repress transcription. ..