Gene Symbol: SWI5
Description: DNA-binding transcription factor SWI5
Alias: DNA-binding transcription factor SWI5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cosma M, Tanaka T, Nasmyth K. Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter. Cell. 1999;97:299-311 pubmed
    ..Swi5p remains at HO for only 5 min. Swi/Snf's and SAGA's subsequent persistence at HO is self sustaining and constitutes an "epigenetic memory" of HO's transient interaction with Swi5p. ..
  2. Toyn J, Johnson A, Donovan J, Toone W, Johnston L. The Swi5 transcription factor of Saccharomyces cerevisiae has a role in exit from mitosis through induction of the cdk-inhibitor Sic1 in telophase. Genetics. 1997;145:85-96 pubmed
    ..We show that the cell cycle timing of SIC1 expression depends on the transcription factor Swi5, and that Swi5-dependent SIC1 expression begins during telophase...
  3. Knapp D, Bhoite L, Stillman D, Nasmyth K. The transcription factor Swi5 regulates expression of the cyclin kinase inhibitor p40SIC1. Mol Cell Biol. 1996;16:5701-7 pubmed
    ..Mutations in three loci (SIC1, SWI5, and RIC3) were identified. We have shown that high SIC1 transcript levels at late M phase depend on Swi5...
  4. Visintin R, Craig K, Hwang E, Prinz S, Tyers M, Amon A. The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation. Mol Cell. 1998;2:709-18 pubmed
    ..Cdc14 dephosphorylates Sic1, a Cdk inhibitor, and Swi5, a transcription factor for SIC1, and induces degradation of mitotic cyclins, likely by dephosphorylating the ..
  5. McBride H, Yu Y, Stillman D. Distinct regions of the Swi5 and Ace2 transcription factors are required for specific gene activation. J Biol Chem. 1999;274:21029-36 pubmed
    b>Swi5 and Ace2 are cell cycle-regulated transcription factors that activate expression of early G(1)-specific genes in Saccharomyces cerevisiae...
  6. Voth W, Yu Y, Takahata S, Kretschmann K, Lieb J, Parker R, et al. Forkhead proteins control the outcome of transcription factor binding by antiactivation. EMBO J. 2007;26:4324-34 pubmed
    Transcription factors with identical DNA-binding specificity often activate different genes in vivo. Yeast Ace2 and Swi5 are such activators, with targets we classify as Swi5-only, Ace2-only, or both. We define two unique regulatory modes...
  7. Krebs J, Fry C, Samuels M, Peterson C. Global role for chromatin remodeling enzymes in mitotic gene expression. Cell. 2000;102:587-98 pubmed
    ..We propose that SWI/SNF and Gcn5p are globally required for mitotic gene expression due to the condensed state of mitotic chromatin. ..
  8. Brazas R, Stillman D. Identification and purification of a protein that binds DNA cooperatively with the yeast SWI5 protein. Mol Cell Biol. 1993;13:5524-37 pubmed
    The Saccharomyces cerevisiae SWI5 gene encodes a zinc finger protein required for the expression of the HO gene. A protein fusion between glutathione S-transferase and SWI5 was expressed in Escherichia coli and purified...
  9. Batenchuk C, Tepliakova L, Kaern M. Identification of response-modulated genetic interactions by sensitivity-based epistatic analysis. BMC Genomics. 2010;11:493 pubmed publisher
    ..While the method was developed for growth phenotype, it should apply equally well for other phenotypes, including the expression of fluorescent reporters. ..

More Information


  1. Brazas R, Bhoite L, Murphy M, Yu Y, Chen Y, Neklason D, et al. Determining the requirements for cooperative DNA binding by Swi5p and Pho2p (Grf10p/Bas2p) at the HO promoter. J Biol Chem. 1995;270:29151-61 pubmed
    ..The mechanism of the cooperative DNA binding by Swi5p and Pho2p is discussed. ..
  2. Stern M, Jensen R, Herskowitz I. Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol. 1984;178:853-68 pubmed
    ..These results indicate that the SWI genes function in some way as positive regulators of HO expression and have additional cellular roles. ..
  3. Archambault V, Li C, Tackett A, Wasch R, Chait B, Rout M, et al. Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit. Mol Biol Cell. 2003;14:4592-604 pubmed
    ..We conclude, therefore, that although both Cdc6 and Sic1 have the potential to facilitate mitotic exit by inhibiting Clb2-Cdk, mitotic exit nevertheless does not require any identified stoichiometric inhibitor of Cdk activity. ..
  4. Di Talia S, Wang H, Skotheim J, Rosebrock A, Futcher B, Cross F. Daughter-specific transcription factors regulate cell size control in budding yeast. PLoS Biol. 2009;7:e1000221 pubmed publisher
    ..This work demonstrates mechanistically how asymmetric localization of cell fate determinants results in cell-type-specific regulation of the cell cycle. ..
  5. Breeden L, Mikesell G. Cell cycle-specific expression of the SWI4 transcription factor is required for the cell cycle regulation of HO transcription. Genes Dev. 1991;5:1183-90 pubmed
    ..High-level constitutive synthesis of SWI4 also suppresses swi5 and swi6 mutations, suggesting that SWI4 is the predominant activator of HO transcription and that mutations in ..
  6. Nasmyth K, Stillman D, Kipling D. Both positive and negative regulators of HO transcription are required for mother-cell-specific mating-type switching in yeast. Cell. 1987;48:579-87 pubmed
    ..characterization of mutations in two new genes called SDI1 and SDI2, which partially suppress the requirement for SWI5 and which cause daughter cells to express HO...
  7. Zhang S, Skalsky Y, Garfinkel D. MGA2 or SPT23 is required for transcription of the delta9 fatty acid desaturase gene, OLE1, and nuclear membrane integrity in Saccharomyces cerevisiae. Genetics. 1999;151:473-83 pubmed
    ..We have isolated a gene-specific activator, SWI5, and the Delta9 fatty acid desaturase of yeast, OLE1, as multicopy suppressors of an mga2Delta spt23 temperature-..
  8. Meitinger F, Richter H, Heisel S, Hub B, Seufert W, Pereira G. A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity. PLoS Biol. 2013;11:e1001495 pubmed publisher
    ..Our findings provide unexpected insights into the temporal control of cytokinesis and describe the importance of a Gps1-dependent mechanism for highly accurate polarity switching between two closely connected locations. ..
  9. Perez Martin J, Johnson A. The C-terminal domain of Sin1 interacts with the SWI-SNF complex in yeast. Mol Cell Biol. 1998;18:4157-64 pubmed
    ..Based on these and additional results, we propose that Sin1 acts as a regulatable bridge between the SWI-SNF complex and the nucleosome. ..
  10. Aerne B, Johnson A, Toyn J, Johnston L. Swi5 controls a novel wave of cyclin synthesis in late mitosis. Mol Biol Cell. 1998;9:945-56 pubmed
    We have shown previously that the Swi5 transcription factor regulates the expression of the SIC1 Cdk inhibitor in late mitosis. This suggests that Swi5 might control other genes with roles in ending mitosis...
  11. Kõivomägi M, Ord M, Iofik A, Valk E, Venta R, Faustova I, et al. Multisite phosphorylation networks as signal processors for Cdk1. Nat Struct Mol Biol. 2013;20:1415-24 pubmed publisher
  12. Voth W, Olsen A, Sbia M, Freedman K, Stillman D. ACE2, CBK1, and BUD4 in budding and cell separation. Eukaryot Cell. 2005;4:1018-28 pubmed
    ..b>SWI5 encodes a transcription factor paralogous to ACE2...
  13. Cosma M, Panizza S, Nasmyth K. Cdk1 triggers association of RNA polymerase to cell cycle promoters only after recruitment of the mediator by SBF. Mol Cell. 2001;7:1213-20 pubmed
    ..The first is triggered by inactivation of Cdk1 at the end of mitosis, which promotes import into the nucleus of the Swi5 transcription factor...
  14. Takahata S, Yu Y, Stillman D. FACT and Asf1 regulate nucleosome dynamics and coactivator binding at the HO promoter. Mol Cell. 2009;34:405-15 pubmed publisher
    ..The Swi/Snf, SAGA, and Mediator coactivators bind first to the far upstream promoter region and subsequently to a promoter proximal region, and FACT and Asf1 are both required for this coactivator re-recruitment. ..
  15. Neely K, Hassan A, Wallberg A, Steger D, Cairns B, Wright A, et al. Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays. Mol Cell. 1999;4:649-55 pubmed
    ..The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts...
  16. Dresser M, Ewing D, Conrad M, Dominguez A, Barstead R, Jiang H, et al. DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics. 1997;147:533-44 pubmed
    ..Interestingly, the pattern on CHEF gels of chromosome fragments that result from meiotic DNA double-strand break formation is different in DMC1 mutant strains from that seen in rad50S strains. ..
  17. Bhoite L, Stillman D. Residues in the Swi5 zinc finger protein that mediate cooperative DNA binding with the Pho2 homeodomain protein. Mol Cell Biol. 1998;18:6436-46 pubmed
    The Swi5 zinc finger and the Pho2 homeodomain DNA-binding proteins bind cooperatively to the HO promoter. Pho2 (also known as Bas2 or Grf10) activates transcription of diverse genes, acting with multiple distinct DNA-binding proteins...
  18. Kishi T, Ikeda A, Koyama N, Fukada J, Nagao R. A refined two-hybrid system reveals that SCF(Cdc4)-dependent degradation of Swi5 contributes to the regulatory mechanism of S-phase entry. Proc Natl Acad Sci U S A. 2008;105:14497-502 pubmed publisher
    ..system to allow screening Cdc4-substrate interactions under conditions of substrate stabilization, and identified Swi5 as a substrate of the SCF(Cdc4) complex...
  19. Parnell E, Yu Y, Lucena R, Yoon Y, Bai L, Kellogg D, et al. The Rts1 regulatory subunit of PP2A phosphatase controls expression of the HO endonuclease via localization of the Ace2 transcription factor. J Biol Chem. 2014;289:35431-7 pubmed publisher
    The RTS1 gene encodes a subunit of the PP2A phosphatase that regulates cell cycle progression. Ace2 and Swi5 are cell cycle-regulated transcription factors, and we recently showed that phosphorylation of Ace2 and Swi5 is altered in an ..
  20. Stillman D, Bankier A, Seddon A, Groenhout E, Nasmyth K. Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene. EMBO J. 1988;7:485-94 pubmed
    ..It has been demonstrated that the SWI5 gene, which is an activator of HO expression, plays a critical role in this differential mother/daughter expression ..
  21. Taberner F, Quilis I, Sendra J, Bañó M, Igual J. Regulation of cell cycle transcription factor Swi5 by karyopherin Msn5. Biochim Biophys Acta. 2012;1823:959-70 pubmed publisher
    ..We determined that Msn5 controlled subcellular localization of the mitotic exit transcription factor Swi5, since it was required for Swi5 nuclear export. Msn5 physically interacted with the N-terminal end of Swi5...
  22. Neely K, Hassan A, Brown C, Howe L, Workman J. Transcription activator interactions with multiple SWI/SNF subunits. Mol Cell Biol. 2002;22:1615-25 pubmed
    ..Thus, three distinct subunits of the SWI/SNF complex contribute to its interactions with activation domains. ..
  23. Stillman D, Dorland S, Yu Y. Epistasis analysis of suppressor mutations that allow HO expression in the absence of the yeast SW15 transcriptional activator. Genetics. 1994;136:781-8 pubmed
    ..We have also identified a new swi5 suppressor, RGR1. The RGR1 gene was identified originally as a negative regulator of SUC2...
  24. Measday V, McBride H, Moffat J, Stillman D, Andrews B. Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast. Mol Microbiol. 2000;35:825-34 pubmed
    ..Pcl2-Pho85, we performed a two-hybrid screen using the Pcl2 cyclin as bait and recovered the transcription factor Swi5 as a Pcl2-interacting protein...
  25. McBride H, Brazas R, Yu Y, Nasmyth K, Stillman D. Long-range interactions at the HO promoter. Mol Cell Biol. 1997;17:2669-78 pubmed
    The SWI5 gene encodes a zinc finger DNA-binding protein required for the transcriptional activation of the yeast HO gene. There are two Swi5p binding sites in the HO promoter, site A at -1800 and site B at -1300...
  26. Wu Y, Du J, Xu G, Jiang L. The transcription factor Ace2 and its paralog Swi5 regulate ethanol production during static fermentation through their targets Cts1 and Rps4a in Saccharomyces cerevisiae. FEMS Yeast Res. 2016;16: pubmed publisher
    ..for 77 yeast genes encoding nonessential transcription factors, and found that deletion mutants for ACE2 and SWI5 showed dramatically increased ethanol yields...
  27. Cross F, Schroeder L, Bean J. Phosphorylation of the Sic1 inhibitor of B-type cyclins in Saccharomyces cerevisiae is not essential but contributes to cell cycle robustness. Genetics. 2007;176:1541-55 pubmed
  28. Bhoite L, Yu Y, Stillman D. The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II. Genes Dev. 2001;15:2457-69 pubmed
    ..Binding of the early G1 transcription factor Swi5 to the distal URS1 element of the HO promoter initiates a cascade of events through recruitment of the Swi/Snf and ..
  29. Yu Y, Eriksson P, Stillman D. Architectural transcription factors and the SAGA complex function in parallel pathways to activate transcription. Mol Cell Biol. 2000;20:2350-7 pubmed
    ..These studies underscore the critical role of chromatin structure in regulating HO gene expression. ..
  30. Hahn S, Maurer P, Caesar S, Schlenstedt G. Classical NLS proteins from Saccharomyces cerevisiae. J Mol Biol. 2008;379:678-94 pubmed publisher
    ..We show for the first time that endogenous S. cerevisiae proteins Prp20, Cdc6, Swi5, Cdc45, and Clb2 fulfill all of these criteria identifying them as authentic yeast cNLS cargos...
  31. Brazas R, Stillman D. The Swi5 zinc-finger and Grf10 homeodomain proteins bind DNA cooperatively at the yeast HO promoter. Proc Natl Acad Sci U S A. 1993;90:11237-41 pubmed
    b>SWI5 encodes a zinc-finger protein required for expression of the yeast HO gene...