Gene Symbol: SUP45
Description: translation termination factor eRF1
Alias: SAL4, SUP1, SUP47, translation termination factor eRF1
Species: Saccharomyces cerevisiae S288c
Products:     SUP45

Top Publications

  1. Stansfield I, Kushnirov V, Jones K, Tuite M. A conditional-lethal translation termination defect in a sup45 mutant of the yeast Saccharomyces cerevisiae. Eur J Biochem. 1997;245:557-63 pubmed
    Genetic studies have indicated that the product of the yeast SUP45 gene encodes a component of the translational-termination machinery...
  2. Zhuravleva G, Moskalenko S, Murina O, Inge Vechtomov S. [Viable nonsense mutants for the SUP45 gene in the yeast Saccharomyces cerevisiae are lethal at increased temperature]. Genetika. 2007;43:1363-71 pubmed
    Nonlethal nonsense mutations obtained earlier in the essential gene SUP45 encoding the translation termination eRFI factor in the yeast Saccharomyces cerevisiae were further characterized...
  3. Shoemaker C, Eyler D, Green R. Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. Science. 2010;330:369-72 pubmed publisher
  4. Stansfield I, Grant G, Akhmaloka -, Tuite M. Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination. Mol Microbiol. 1992;6:3469-78 pubmed
    The SAL4 gene of the yeast Saccharomyces cerevisiae encodes a novel translation factor (Sal4p) involved in maintaining translational fidelity...
  5. Paushkin S, Kushnirov V, Smirnov V, Ter Avanesyan M. Interaction between yeast Sup45p (eRF1) and Sup35p (eRF3) polypeptide chain release factors: implications for prion-dependent regulation. Mol Cell Biol. 1997;17:2798-805 pubmed
    The SUP45 and SUP35 genes of Saccharomyces cerevisiae encode polypeptide chain release factors eRF1 and eRF3, respectively...
  6. Eurwilaichitr L, Graves F, Stansfield I, Tuite M. The C-terminus of eRF1 defines a functionally important domain for translation termination in Saccharomyces cerevisiae. Mol Microbiol. 1999;32:485-96 pubmed
  7. Merritt G, Naemi W, Mugnier P, Webb H, Tuite M, von der Haar T. Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast. Nucleic Acids Res. 2010;38:5479-92 pubmed publisher
    ..We reassess current models of stop-codon recognition by eRF1 in the light of these new data. ..
  8. Hatin I, Fabret C, Rousset J, Namy O. Molecular dissection of translation termination mechanism identifies two new critical regions in eRF1. Nucleic Acids Res. 2009;37:1789-98 pubmed publisher
    ..In Saccharomyces cerevisiae, these proteins are encoded by the genes SUP45 and SUP35, respectively...
  9. Gross T, Siepmann A, Sturm D, Windgassen M, Scarcelli J, Seedorf M, et al. The DEAD-box RNA helicase Dbp5 functions in translation termination. Science. 2007;315:646-9 pubmed
    ..Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events. ..

More Information


  1. Shoemaker C, Green R. Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast. Proc Natl Acad Sci U S A. 2011;108:E1392-8 pubmed publisher
    ..These results establish a model in which eukaryotic termination and recycling are not clearly demarcated events, as they are in bacteria, but coupled stages of the same release-factor mediated process...
  2. Czaplinski K, Ruiz Echevarria M, Paushkin S, Han X, Weng Y, Perlick H, et al. The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs. Genes Dev. 1998;12:1665-77 pubmed
  3. Vishveshwara N, Bradley M, Liebman S. Sequestration of essential proteins causes prion associated toxicity in yeast. Mol Microbiol. 2009;73:1101-14 pubmed publisher
    ..Overexpression of Sup45p, but not other tested essential Sup35p binding partners, caused rescue. Sup45-GFPp formed puncta that colocalized with large [PSI(+)] Sup35-RFPp aggregates in cells overexpressing Sup35p, and ..
  4. Kobayashi T, Funakoshi Y, Hoshino S, Katada T. The GTP-binding release factor eRF3 as a key mediator coupling translation termination to mRNA decay. J Biol Chem. 2004;279:45693-700 pubmed
    ..The termination reaction itself is not sufficient, but eRF3 is essential for triggering mRNA decay. Thus, eRF3 is a key mediator that transduces termination signal to mRNA decay. ..
  5. Bolger T, Folkmann A, Tran E, Wente S. The mRNA export factor Gle1 and inositol hexakisphosphate regulate distinct stages of translation. Cell. 2008;134:624-33 pubmed publisher
    ..However, Gle1 also independently mediates initiation. Thus, Gle1 is uniquely positioned to coordinate the mRNA export and translation mechanisms. These results directly impact models for perturbation of Gle1 function in pathophysiology. ..
  6. Chabelskaya S, Gryzina V, Moskalenko S, Le Goff C, Zhouravleva G. Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae. BMC Mol Biol. 2007;8:71 pubmed
    ..Translation termination factors eRF1 (Sup45) and eRF3 (Sup35) participate not only in the last step of protein synthesis but also in mRNA degradation and ..
  7. Stansfield I, Jones K, Kushnirov V, Dagkesamanskaya A, Poznyakovski A, Paushkin S, et al. The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J. 1995;14:4365-73 pubmed
    The product of the yeast SUP45 gene (Sup45p) is highly homologous to the Xenopus eukaryote release factor 1 (eRF1), which has release factor activity in vitro...
  8. Khoshnevis S, Gross T, Rotte C, Baierlein C, Ficner R, Krebber H. The iron-sulphur protein RNase L inhibitor functions in translation termination. EMBO Rep. 2010;11:214-9 pubmed publisher
    ..we show that Rli1 interacts physically with the translation termination factors eukaryotic release factor 1 (eRF1)/Sup45 and eRF3/Sup35 in Saccharomyces cerevisiae...
  9. Akhmaloka -, Susilowati P, Subandi -, Madayanti F. Mutation at tyrosine in AMLRY (GILRY like) motif of yeast eRF1 on nonsense codons suppression and binding affinity to eRF3. Int J Biol Sci. 2008;4:87-95 pubmed
    ..The data suggested that increasing stop codon suppression and decreasing of the binding affinity of eRF1(Y410S) were probably due to the slight modification on the structure of the C-terminal domain. ..
  10. Bailleul P, Newnam G, Steenbergen J, Chernoff Y. Genetic study of interactions between the cytoskeletal assembly protein sla1 and prion-forming domain of the release factor Sup35 (eRF3) in Saccharomyces cerevisiae. Genetics. 1999;153:81-94 pubmed
    ..We hypothesize that Sup35N is involved in regulatory interactions with intracellular structural networks, and [PSI] prion may be formed as a by-product of this process. ..
  11. Urakov V, Valouev I, Lewitin E, Paushkin S, Kosorukov V, Kushnirov V, et al. Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiae. BMC Mol Biol. 2001;2:9 pubmed
    ..This protein possesses a zinc finger domain characteristic of the TRIAD proteins of higher eukaryotes, and this is a first observation of such protein being involved in translation. ..
  12. Ebihara K, Nakamura Y. C-terminal interaction of translational release factors eRF1 and eRF3 of fission yeast: G-domain uncoupled binding and the role of conserved amino acids. RNA. 1999;5:739-50 pubmed
    ..e., probably uncoupled with GTP hydrolysis), whereas aminoacyl-tRNA binding depends on that of EF-Tu/EF-1alpha(i.e., coupled with GTP hydrolysis), which sheds some light on the mechanism of eRF3 function. ..
  13. Valouev I, Urakov V, Kochneva Pervukhova N, Smirnov V, Ter Avanesyan M. Translation termination factors function outside of translation: yeast eRF1 interacts with myosin light chain, Mlc1p, to effect cytokinesis. Mol Microbiol. 2004;53:687-96 pubmed
    The translation termination factor eRF1 recognizes stop codons at the A site of the ribosome and induces peptidyl-tRNA hydrolysis at the peptidyl transferase centre...
  14. Zhuravleva G, Gryzina V. [The influence of UPF genes on the severity of SUP45 mutations]. Mol Biol (Mosk). 2012;46:285-97 pubmed
    ..Here we show that deletion of either UPF1 or UPF2 increased viability of sup45 mutants, while effect of UPF3 deletion is allele-specific...
  15. Betney R, de Silva E, Mertens C, Knox Y, Krishnan J, Stansfield I. Regulation of release factor expression using a translational negative feedback loop: a systems analysis. RNA. 2012;18:2320-34 pubmed publisher
    The essential eukaryote release factor eRF1, encoded by the yeast SUP45 gene, recognizes stop codons during ribosomal translation...
  16. Eyler D, Wehner K, Green R. Eukaryotic release factor 3 is required for multiple turnovers of peptide release catalysis by eukaryotic release factor 1. J Biol Chem. 2013;288:29530-8 pubmed publisher
    ..A role for eRF3 as an escort for eRF1 into its fully accommodated state is easily reconciled with its close sequence similarity to the translational GTPase EFTu. ..
  17. Polevoda B, Span L, Sherman F. The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p. J Biol Chem. 2006;281:2562-71 pubmed
    ..Most interestingly, the mtq2-Delta was significantly more resistant to the anti-microtubule drugs thiabendazole and benomyl, suggesting that Mtq2p may also methylate certain microtubule-related proteins. ..
  18. Pezza J, Villali J, Sindi S, Serio T. Amyloid-associated activity contributes to the severity and toxicity of a prion phenotype. Nat Commun. 2014;5:4384 pubmed publisher
    ..Thus, amyloid heterogeneity is a crucial contributor to protein-only phenotypes. ..
  19. Fang N, Chan G, Zhu M, Comyn S, Persaud A, Deshaies R, et al. Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat Cell Biol. 2014;16:1227-37 pubmed publisher
    ..Our results support a bipartite recognition mechanism combining direct and chaperone-dependent ubiquitylation of misfolded cytosolic proteins by Rsp5. ..
  20. Roque S, Cerciat M, Gaugué I, Mora L, Floch A, de Zamaroczy M, et al. Interaction between the poly(A)-binding protein Pab1 and the eukaryotic release factor eRF3 regulates translation termination but not mRNA decay in Saccharomyces cerevisiae. RNA. 2015;21:124-34 pubmed publisher
    ..Our results show that in Saccharomyces cerevisiae the role of the Pab1 C-terminal domain in mRNA stability is independent of eRF3 and the association of these two factors negatively regulates translation termination. ..
  21. Fabret C, Cosnier B, Lekomtsev S, Gillet S, Hatin I, Le Marechal P, et al. A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability. BMC Mol Biol. 2008;9:22 pubmed publisher
    ..They also raise interesting questions about the relation between GTPase activity of Sup35 and its essential function in yeast. ..
  22. Valouev I, Fominov G, Sokolova E, Smirnov V, Ter Avanesyan M. Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast. BMC Mol Biol. 2009;10:60 pubmed publisher
    ..These genes act as dosage suppressors of a synthetic growth defect caused by some mutations in the SUP45 and SUP35 genes encoding eRF1 and eRF3, respectively...
  23. Matveenko A, Zemlianko O, Zhuravleva G. [The identification of Saccharomyces cerevisiae genes leading to synthetic lethality of prion [PSI+] with Sup45 mutations]. Mol Biol (Mosk). 2013;47:609-17 pubmed
    ..we proposed a test system allowing to perform search for genes that influence the properties of the Sup35 and Sup45 protein...
  24. Nizhnikov A, Magomedova Z, Rubel A, Kondrashkina A, Inge Vechtomov S, Galkin A. [NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes. Curr Genet. 2012;58:35-47 pubmed publisher
    ..screen to identify genes that impact the phenotypic effects of [NSI(+)], we found that the SUP35 and SUP45 genes encoding the translation termination factors eRF3 and eRF1, respectively, modulate nonsense suppression in [..
  25. Petrova A, Kiktev D, Askinazi O, Chabelskaya S, Moskalenko S, Zemlyanko O, et al. The translation termination factor eRF1 (Sup45p) of Saccharomyces cerevisiae is required for pseudohyphal growth and invasion. FEMS Yeast Res. 2015;15:fov033 pubmed publisher
    Mutations in the essential genes SUP45 and SUP35, encoding yeast translation termination factors eRF1 and eRF3, respectively, lead to a wide range of phenotypes and affect various cell processes...
  26. Grousl T, Ivanov P, Malcová I, Pompach P, Frýdlová I, Slaba R, et al. Heat shock-induced accumulation of translation elongation and termination factors precedes assembly of stress granules in S. cerevisiae. PLoS ONE. 2013;8:e57083 pubmed publisher
    ..We suggest that identification of translation elongation and termination factors in SGs might help to understand the mechanism of the eIF2? factor phosphorylation-independent repression of translation and SGs assembly. ..
  27. Conard S, Buckley J, Dang M, Bedwell G, Carter R, Khass M, et al. Identification of eRF1 residues that play critical and complementary roles in stop codon recognition. RNA. 2012;18:1210-21 pubmed publisher
    ..In particular, changes in the YCF motif, rather than the TASNIKS motif, correlated most consistently with variant code stop codon selectivity. ..
  28. Derkatch I, Bradley M, Liebman S. Overexpression of the SUP45 gene encoding a Sup35p-binding protein inhibits the induction of the de novo appearance of the [PSI+] prion. Proc Natl Acad Sci U S A. 1998;95:2400-5 pubmed
    ..However, another non-Mendelian determinant, [PIN+], is required for this induction. We now show that SUP45 overexpression inhibits the induction of [PSI+] by Sup35p overproduction in [PIN+] strains, but has no effect on ..
  29. Murina O, Moskalenko S, Zhuravleva G. [Overexpression of genes encoding tRNA(Tyr) AND tRNA(Gln) improves viability of nonsense mutants in SUP45 gene in yeast Saccharomyces cerevisiae]. Mol Biol (Mosk). 2010;44:301-10 pubmed
    ..In yeast Saccharomyces cerevisiae nonsense-mutants containing premature stop-codon in mRNA of the essential SUP45 gene were obtained...
  30. Kiktev D, Moskalenko S, Murina O, Baudin Baillieu A, Rousset J, Zhouravleva G. The paradox of viable sup45 STOP mutations: a necessary equilibrium between translational readthrough, activity and stability of the protein. Mol Genet Genomics. 2009;282:83-96 pubmed publisher
    ..of yeast cells bearing premature termination codons (PTCs) in the essential gene SUP45 encoding translation termination factor eRF1. Using a dual reporter system we compared readthrough efficiency of the natural termination codon ..
  31. Blanchet S, Rowe M, von der Haar T, Fabret C, Demais S, Howard M, et al. New insights into stop codon recognition by eRF1. Nucleic Acids Res. 2015;43:3298-308 pubmed publisher
    ..By combining our genetic data with a structural analysis of eRF1 mutants, we were able to formulate a new model in which the stop codon interacts with eRF1 through the P1 pocket. ..
  32. Saito K, Ito K. Genetic analysis of L123 of the tRNA-mimicking eukaryote release factor eRF1, an amino acid residue critical for discrimination of stop codons. Nucleic Acids Res. 2015;43:4591-601 pubmed publisher
    ..Our results provide insights into the molecular mechanisms underlying stop codon discrimination by a tRNA-mimicking protein on the ribosome. ..
  33. Beznosková P, Cuchalová L, Wagner S, Shoemaker C, Gunisova S, von der Haar T, et al. Translation initiation factors eIF3 and HCR1 control translation termination and stop codon read-through in yeast cells. PLoS Genet. 2013;9:e1003962 pubmed publisher
    ..Together our work characterizes novel roles of eIF3 and HCR1 in stop codon recognition, defining a communication bridge between the initiation and termination/recycling phases of translation. ..
  34. Heurgué Hamard V, Champ S, Mora L, Merkulova Rainon T, Merkoulova Rainon T, Kisselev L, et al. The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 is methylated by the product of the YDR140w gene. J Biol Chem. 2005;280:2439-45 pubmed
    ..erf3.gtp. ..
  35. Nizhnikov A, Ryzhova T, Volkov K, Zadorsky S, Sopova J, Inge Vechtomov S, et al. Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae. PLoS Genet. 2016;12:e1006504 pubmed publisher
    ..We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression...
  36. Tomson B, Crisucci E, Heisler L, Gebbia M, Nislow C, Arndt K. Effects of the Paf1 complex and histone modifications on snoRNA 3'-end formation reveal broad and locus-specific regulation. Mol Cell Biol. 2013;33:170-82 pubmed publisher
    ..cerevisiae, implicates the participation of transcriptional proteins and histone modifications in this process, and suggests that the Paf1C contributes to the fine tuning of nuanced levels of regulation that exist at individual loci. ..
  37. Zhouravleva G, Petrova A. The role of translation termination factor eRF1 in the regulation of pseudohyphal growth in Saccharomyces cerevisiae cells. Dokl Biochem Biophys. 2010;433:209-11 pubmed publisher
  38. Zhang C, Wang X, Park S, Chiang Y, Xi W, Laue T, et al. Only a subset of the PAB1-mRNP proteome is present in mRNA translation complexes. Protein Sci. 2014;23:1036-49 pubmed publisher
    ..These data inform that AU-FDS can clarify protein complex identification that remains undetermined after typical immunoprecipitation and mass spectrometric analyses. ..
  39. Kiktev D, Vechtomov S, Zhouravleva G. Prion-dependent lethality of sup45 mutants in Saccharomyces cerevisiae. Prion. 2007;1:136-43 pubmed
    In yeast Saccharomyces cerevisiae translation termination factors eRF1 (Sup45) and eRF3 (Sup35) are encoded by the essential genes SUP45 and SUP35 respectively...
  40. Kondrashkina A, Antonets K, Galkin A, Nizhnikov A. [Prion-like determinant [NSI+] decreases expression of the SUP45 gene in Saccharomyces cerevisiae]. Mol Biol (Mosk). 2014;48:790-6 pubmed
    ..As a result of geneticscreen, we demonstrated that an increase in the expression of SUP45 encoding the eRF1 release factor (Sup45), masks, but does not eliminate nonsense suppression in the [NSI+] strains...
  41. Figaro S, Scrima N, Buckingham R, Heurgué Hamard V. HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1. FEBS Lett. 2008;582:2352-6 pubmed publisher
    ..Here we show that the human proteins methylate human and yeast eRF1.eRF3.GTP in vitro, and that the MTase catalytic subunit can complement the growth defect of yeast strains deleted for mtq2. ..