STU2

Summary

Gene Symbol: STU2
Description: Stu2p
Alias: Stu2p
Species: Saccharomyces cerevisiae S288c
Products:     STU2

Top Publications

  1. Usui T, Maekawa H, Pereira G, Schiebel E. The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. EMBO J. 2003;22:4779-93 pubmed
    The yeast protein Stu2 belongs to the XMAP215 family of conserved microtubule-binding proteins which regulate microtubule plus end dynamics...
  2. Pearson C, Maddox P, Zarzar T, Salmon E, Bloom K. Yeast kinetochores do not stabilize Stu2p-dependent spindle microtubule dynamics. Mol Biol Cell. 2003;14:4181-95 pubmed
    ..proteins and kinetochore function, we identify a regulatory mechanism for spindle microtubule dynamics involving Stu2p and the core kinetochore component, Ndc10p...
  3. Severin F, Habermann B, Huffaker T, Hyman T. Stu2 promotes mitotic spindle elongation in anaphase. J Cell Biol. 2001;153:435-42 pubmed
    ..We show that microtubule stabilization during anaphase requires the microtubule-associated protein Stu2. We further show that the activity of Stu2 is opposed by the activity of the kinesin-related protein Kip3...
  4. Wang P, Huffaker T. Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body. J Cell Biol. 1997;139:1271-80 pubmed
    ..b>Stu2p localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules...
  5. Miller R, Cheng S, Rose M. Bim1p/Yeb1p mediates the Kar9p-dependent cortical attachment of cytoplasmic microtubules. Mol Biol Cell. 2000;11:2949-59 pubmed
    ..a two-hybrid screen was conducted with the use of full-length Kar9p as bait, and three genes were identified: BIM1, STU2, and KAR9 itself. STU2 encodes a component of the spindle pole body...
  6. Slep K, Vale R. Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1. Mol Cell. 2007;27:976-91 pubmed
    ..Here, we report crystal structures of the tubulin binding domains of XMAP215 (yeast Stu2p and Drosophila Msps), EB1 (yeast Bim1p and human EB1), and CLIP-170 (human), which reveal diverse tubulin binding ..
  7. Chen X, Yin H, Huffaker T. The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly. J Cell Biol. 1998;141:1169-79 pubmed
    We have previously shown that Stu2p is a microtubule-binding protein and a component of the Saccharomyces cerevisiae spindle pole body (SPB). Here we report the identification of Spc72p, a protein that interacts with Stu2p...
  8. He X, Rines D, Espelin C, Sorger P. Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell. 2001;106:195-206 pubmed
    ..The proteins include both molecular motors and microtubule associated proteins (MAPs), implying that motors and MAPs function together in binding chromosomes to spindle microtubules. ..
  9. Kosco K, Pearson C, Maddox P, Wang P, Adams I, Salmon E, et al. Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast. Mol Biol Cell. 2001;12:2870-80 pubmed
    b>Stu2p is a member of a conserved family of microtubule-binding proteins and an essential protein in yeast. Here, we report the first in vivo analysis of microtubule dynamics in cells lacking a member of this protein family...

More Information

Publications30

  1. Wolyniak M, Blake Hodek K, Kosco K, Hwang E, You L, Huffaker T. The regulation of microtubule dynamics in Saccharomyces cerevisiae by three interacting plus-end tracking proteins. Mol Biol Cell. 2006;17:2789-98 pubmed
    ..Here we investigate the physical and functional interactions among three +TIPs in S. cerevisiae, Stu2, Bik1, and Bim1...
  2. Liakopoulos D, Kusch J, Grava S, Vogel J, Barral Y. Asymmetric loading of Kar9 onto spindle poles and microtubules ensures proper spindle alignment. Cell. 2003;112:561-74 pubmed
    ..In turn, asymmetric distribution of Kar9 ensures that only one pole orients toward the bud. Our results indicate that Cdk1-dependent spindle asymmetry ensures proper alignment of the mitotic spindle with the cell division axis. ..
  3. Al Bassam J, van Breugel M, Harrison S, Hyman A. Stu2p binds tubulin and undergoes an open-to-closed conformational change. J Cell Biol. 2006;172:1009-22 pubmed
    b>Stu2p from budding yeast belongs to the conserved Dis1/XMAP215 family of microtubule-associated proteins (MAPs). The common feature of proteins in this family is the presence of HEAT repeat-containing TOG domains near the NH2 terminus...
  4. Ayaz P, Ye X, Huddleston P, Brautigam C, Rice L. A TOG:??-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase. Science. 2012;337:857-60 pubmed publisher
    b>Stu2p/XMAP215/Dis1 family proteins are evolutionarily conserved regulatory factors that use ??-tubulin-interacting tumor overexpressed gene (TOG) domains to catalyze fast microtubule growth...
  5. Haase K, Fox J, Byrnes A, Adikes R, Speed S, Haase J, et al. Stu2 uses a 15-nm parallel coiled coil for kinetochore localization and concomitant regulation of the mitotic spindle. Mol Biol Cell. 2018;29:285-294 pubmed publisher
    ..We present the crystal structure of the Saccharomyces cerevisiae Stu2 C-terminal domain, revealing a 15-nm parallel homodimeric coiled coil...
  6. van der Vaart B, Fischböck J, Mieck C, Pichler P, Mechtler K, Medema R, et al. TORC1 signaling exerts spatial control over microtubule dynamics by promoting nuclear export of Stu2. J Cell Biol. 2017;216:3471-3484 pubmed publisher
    ..TORC1 subunit Kog1/Raptor, which in turn allow the TORC1 proximal kinase Sch9/S6K1 to regulate the MT polymerase Stu2/XMAP215...
  7. Titos I, Ivanova T, Mendoza M. Chromosome length and perinuclear attachment constrain resolution of DNA intertwines. J Cell Biol. 2014;206:719-33 pubmed publisher
    ..Furthermore, anaphase Stu2-dependent microtubule dynamics are critical for separation of long chromosomes...
  8. Park C, Park J, Karpova T, Soung N, Yu L, Song S, et al. Requirement for the budding yeast polo kinase Cdc5 in proper microtubule growth and dynamics. Eukaryot Cell. 2008;7:444-53 pubmed publisher
    ..both Cdc28 and Cdc5 were required for the proper modification of the spindle pole body components Nud1, Slk19, and Stu2 in vivo. They also phosphorylated these three proteins in vitro...
  9. You L, Gillilan R, Huffaker T. Model for the yeast cofactor A-beta-tubulin complex based on computational docking and mutagensis. J Mol Biol. 2004;341:1343-54 pubmed
    ..Rbl2 and alpha-tubulin share overlapping binding sites on the beta-tubulin molecule providing a structural explanation for the mutually exclusive binding of Rbl2 and alpha-tubulin to beta-tubulin. ..
  10. Cheeseman I, Brew C, Wolyniak M, Desai A, Anderson S, Muster N, et al. Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J Cell Biol. 2001;155:1137-45 pubmed
    ..These and other analyses implicate the four newly identified subunits and the Dam1p complex as a whole in outer kinetochore function where they are well positioned to facilitate the association of chromosomes with spindle microtubules. ..
  11. Liang F, Richmond D, Wang Y. Coordination of chromatid separation and spindle elongation by antagonistic activities of mitotic and S-phase CDKs. PLoS Genet. 2013;9:e1003319 pubmed publisher
    ..We also show that hyperactive S-phase CDK compromises the microtubule localization of Stu2, a processive microtubule polymerase essential for spindle elongation...
  12. Podolski M, Mahamdeh M, Howard J. Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency. J Biol Chem. 2014;289:28087-93 pubmed publisher
    b>Stu2 is the budding yeast member of the XMAP215/Dis1 family of microtubule polymerases...
  13. Miller M, Asbury C, Biggins S. A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments. Cell. 2016;165:1428-1439 pubmed publisher
    ..Here, we show that the XMAP215 family member, Stu2, makes a major contribution to kinetochore-microtubule coupling...
  14. Winsor B, Schiebel E. Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast. 1997;13:399-434 pubmed
  15. Ayaz P, Munyoki S, Geyer E, Piedra F, Vu E, Bromberg R, et al. A tethered delivery mechanism explains the catalytic action of a microtubule polymerase. elife. 2014;3:e03069 pubmed publisher
    b>Stu2p/XMAP215 proteins are essential microtubule polymerases that use multiple ??-tubulin-interacting TOG domains to bind microtubule plus ends and catalyze fast microtubule growth...
  16. Ma L, McQueen J, Cuschieri L, Vogel J, Measday V. Spc24 and Stu2 promote spindle integrity when DNA replication is stalled. Mol Biol Cell. 2007;18:2805-16 pubmed
    ..Overexpression of Stu1, a CLASP-related MT-associated protein or a truncated form of the XMAP215 orthologue Stu2 rescues spc24-9 HU lethality and prevents spindle expansion...
  17. Moore J, Miller R. The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast. Mol Biol Cell. 2007;18:1187-202 pubmed
    ..analysis suggests that this phosphorylation may attenuate the interaction between Kar9p and the XMAP215-homologue Stu2p. We propose that phosphorylation at serine 197 regulates the release of Kar9p from Stu2p at the SPB, either to ..
  18. Krishnan V, Nirantar S, Crasta K, Cheng A, Surana U. DNA replication checkpoint prevents precocious chromosome segregation by regulating spindle behavior. Mol Cell. 2004;16:687-700 pubmed
    ..Instead, the checkpoint deficiency leads to deregulation of microtubule-associated proteins Cin8 and Stu2, which, in the absence of both chromosome cohesion and bipolar attachment of kinetochores to microtubules, induce ..
  19. Gandhi S, Gierlinski M, Mino A, Tanaka K, Kitamura E, Clayton L, et al. Kinetochore-dependent microtubule rescue ensures their efficient and sustained interactions in early mitosis. Dev Cell. 2011;21:920-33 pubmed publisher
    ..This rescue is promoted by Stu2 transfer (ortholog of vertebrate XMAP215/ch-TOG) from the kinetochore to the microtubule end...
  20. Aravamudhan P, Felzer Kim I, Gurunathan K, Joglekar A. Assembling the protein architecture of the budding yeast kinetochore-microtubule attachment using FRET. Curr Biol. 2014;24:1437-46 pubmed publisher
    ..colocalization measurements involving the essential MT-binding kinetochore components: Ndc80, Dam1, Spc105, and Stu2. These measurements reveal that neighboring Ndc80 complexes within the kinetochore are narrowly distributed along ..
  21. Lin H, de Carvalho P, Kho D, Tai C, Pierre P, Fink G, et al. Polyploids require Bik1 for kinetochore-microtubule attachment. J Cell Biol. 2001;155:1173-84 pubmed
    ..The finding that a protein involved in kinetochore-MT attachment is required for the viability of polyploids has potential implications for cancer therapeutics. ..