Gene Symbol: STP22
Description: ubiquitin-binding ESCRT-I subunit protein STP22
Alias: AGS1, VPL15, VPS23, ubiquitin-binding ESCRT-I subunit protein STP22
Species: Saccharomyces cerevisiae S288c
Products:     STP22

Top Publications

  1. Bilodeau P, Winistorfer S, Kearney W, Robertson A, Piper R. Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome. J Cell Biol. 2003;163:237-43 pubmed
    ..We used NMR spectroscopy and mutagenesis studies to map the Ub-binding surface for Vps27 and Vps23. Mutations in Ub that ablate only Vps27 binding or Vps23 binding blocked the ability of Ub to serve as an MVB ..
  2. Bowers K, Lottridge J, Helliwell S, Goldthwaite L, Luzio J, Stevens T. Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic. 2004;5:194-210 pubmed
    ..We propose the formation of a large multimeric complex on the endosome membrane consisting of ESCRTI, ESCRTII, ESCRTIII and other associated proteins. ..
  3. Teo H, Veprintsev D, Williams R. Structural insights into endosomal sorting complex required for transport (ESCRT-I) recognition of ubiquitinated proteins. J Biol Chem. 2004;279:28689-96 pubmed
    The endosomal sorting complex required for transport (ESCRT-I) is a 350-kDa complex of three proteins, Vps23, Vps28, and Vps37...
  4. Kostelansky M, Sun J, Lee S, Kim J, Ghirlando R, Hierro A, et al. Structural and functional organization of the ESCRT-I trafficking complex. Cell. 2006;125:113-26 pubmed
    ..The yeast ESCRT-I complex contains the Vps23, Vps28, and Vps37 proteins, and its assembly is directed by the C-terminal steadiness box of Vps23, the N-terminal ..
  5. Kostelansky M, Schluter C, Tam Y, Lee S, Ghirlando R, Beach B, et al. Molecular architecture and functional model of the complete yeast ESCRT-I heterotetramer. Cell. 2007;129:485-98 pubmed
    ..Yeast ESCRT-I contains four subunits, Vps23, Vps28, Vps37, and Mvb12...
  6. Chu T, Sun J, Saksena S, Emr S. New component of ESCRT-I regulates endosomal sorting complex assembly. J Cell Biol. 2006;175:815-23 pubmed
    ..and demonstrate that Mvb12 binds to the coiled-coil domain of the ESCRT-I subunit vacuolar protein sorting 23 (Vps23)...
  7. Katzmann D, Babst M, Emr S. Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell. 2001;106:145-55 pubmed
    ..In addition, we characterize a 350 kDa complex, ESCRT-I (composed of Vps23, Vps28, and Vps37), that recognizes ubiquitinated MVB cargo and whose function is required for sorting into MVB ..
  8. Babst M, Odorizzi G, Estepa E, Emr S. Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking. Traffic. 2000;1:248-58 pubmed
    ..This may contribute to the tumorigenic phenotype exhibited by the tsg101 mutant fibroblasts. ..
  9. Ren X, Hurley J. Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast. EMBO J. 2011;30:2130-9 pubmed publisher
    ..the Vps27 ESCRT-0 subunit bind to a novel electropositive N-terminal site on the UEV domain of the ESCRT-I subunit Vps23 centred on Trp16...

More Information


  1. Curtiss M, Jones C, Babst M. Efficient cargo sorting by ESCRT-I and the subsequent release of ESCRT-I from multivesicular bodies requires the subunit Mvb12. Mol Biol Cell. 2007;18:636-45 pubmed
    ..It has been shown that ESCRT-I contains the vacuolar protein sorting (Vps) proteins Vps23, Vps28, and Vps37...
  2. Katzmann D, Stefan C, Babst M, Emr S. Vps27 recruits ESCRT machinery to endosomes during MVB sorting. J Cell Biol. 2003;162:413-23 pubmed
    ..Of the class E Vps proteins, both the ESCRT-I complex (composed of the class E proteins Vps23, 28, and 37) and Vps27 (mammalian hepatocyte receptor tyrosine kinase substrate, Hrs) have been shown to interact ..
  3. Shi Y, Stefan C, Rue S, Teis D, Emr S. Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway. Mol Biol Cell. 2011;22:4093-107 pubmed publisher
    ..Moreover, a complex containing the two novel proteins Ere1 and Ere2 mediates cargo-specific recognition by the retromer pathway. ..
  4. Bruns C, McCaffery J, Curwin A, Duran J, Malhotra V. Biogenesis of a novel compartment for autophagosome-mediated unconventional protein secretion. J Cell Biol. 2011;195:979-92 pubmed publisher
    ..Phosphatidylinositol 3 phosphate, the ESCRT (endosomal sorting complex required for transport) protein Vps23, and the autophagy-related proteins Atg8 and Atg9 are recruited to these Grh1-containing membranes, which lack ..
  5. Shields S, Oestreich A, Winistorfer S, Nguyen D, Payne J, Katzmann D, et al. ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting. J Cell Biol. 2009;185:213-24 pubmed publisher
    ..These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps. ..
  6. Svilar D, Dyavaiah M, Brown A, Tang J, Li J, McDonald P, et al. Alkylation sensitivity screens reveal a conserved cross-species functionome. Mol Cancer Res. 2012;10:1580-96 pubmed publisher
    ..cerevisiae) and protein modification systems, including UBE3B and ICMT in human cells or pby1, lip22, stp22 and aim22 in S. cerevisiae...
  7. Zhang F, Gaur N, Hasek J, Kim S, Qiu H, Swanson M, et al. Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4. Mol Cell Biol. 2008;28:6796-818 pubmed publisher
  8. McMurray M, Stefan C, Wemmer M, Odorizzi G, Emr S, Thorner J. Genetic interactions with mutations affecting septin assembly reveal ESCRT functions in budding yeast cytokinesis. Biol Chem. 2011;392:699-712 pubmed publisher
  9. Zhao Y, Du J, Xiong B, Xu H, Jiang L. ESCRT components regulate the expression of the ER/Golgi calcium pump gene PMR1 through the Rim101/Nrg1 pathway in budding yeast. J Mol Cell Biol. 2013;5:336-44 pubmed publisher
    ..Here we show that deletion mutation of ESCRT components Snf7, Snf8, Stp22, Vps20, Vps25, Vps28, or Vps36 activates the calcium/calcineurin signaling in yeast cells, but surprisingly leads ..
  10. Jensen L, Carroll M, Hall M, Harvey C, Beese S, Culotta V. Down-regulation of a manganese transporter in the face of metal toxicity. Mol Biol Cell. 2009;20:2810-9 pubmed publisher
    ..We provide evidence that manganese starvation is sensed within the lumen of the secretory pathway, whereas manganese toxicity is sensed within an extra-Golgi/cytosolic compartment of the cell. ..
  11. Herrador A, Herranz S, Lara D, Vincent O. Recruitment of the ESCRT machinery to a putative seven-transmembrane-domain receptor is mediated by an arrestin-related protein. Mol Cell Biol. 2010;30:897-907 pubmed publisher
    ..that in Saccharomyces cerevisiae, Rim8 binds to both the putative 7TM pH sensor Rim21 and the ESCRT-I subunit Vps23. We show that an SXP motif in Rim8 mediates binding to the Vps23 ubiquitin E2 variant (UEV) domain and that a ..
  12. Tang S, Buchkovich N, Henne W, Banjade S, Kim Y, Emr S. ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis. elife. 2016;5: pubmed publisher
    ..We therefore provide an enhanced understanding for the activation of the spatially unique ESCRT-III-mediated membrane remodeling. ..
  13. Rothfels K, Tanny J, Molnár E, Friesen H, Commisso C, Segall J. Components of the ESCRT pathway, DFG16, and YGR122w are required for Rim101 to act as a corepressor with Nrg1 at the negative regulatory element of the DIT1 gene of Saccharomyces cerevisiae. Mol Cell Biol. 2005;25:6772-88 pubmed
    ..Interestingly, Rim101 was processed in bro1 and doa4 strains but was unable to mediate efficient repression. ..
  14. Mageswaran S, Johnson N, Odorizzi G, Babst M. Constitutively active ESCRT-II suppresses the MVB-sorting phenotype of ESCRT-0 and ESCRT-I mutants. Mol Biol Cell. 2015;26:554-68 pubmed publisher
    ..However, the ESCRT-III/Vps4 system alone is not sufficient for ILV formation but requires cargo sorting mediated by one of the early ESCRTs. ..
  15. Herrador A, Livas D, Soletto L, Becuwe M, Léon S, Vincent O. Casein kinase 1 controls the activation threshold of an α-arrestin by multisite phosphorylation of the interdomain hinge. Mol Biol Cell. 2015;26:2128-38 pubmed publisher
    ..These findings support a model in which CK1-mediated phosphorylation of Rim8 contributes to setting a signaling threshold required to inhibit the RIM pathway at acidic pH. ..
  16. Li Y, Kane T, Tipper C, Spatrick P, Jenness D. Yeast mutants affecting possible quality control of plasma membrane proteins. Mol Cell Biol. 1999;19:3588-99 pubmed
    ..The stp22 mutation (allelic to vps23 [M. Babst and S...
  17. Scott P, Bilodeau P, Zhdankina O, Winistorfer S, Hauglund M, Allaman M, et al. GGA proteins bind ubiquitin to facilitate sorting at the trans-Golgi network. Nat Cell Biol. 2004;6:252-9 pubmed
    ..Thus, GGA proteins fulfill the role of ubiquitin sorting receptors at the TGN...
  18. Nikko E, Andre B. Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase. Eukaryot Cell. 2007;6:1266-77 pubmed
    ..We show that Bro1 interacts with ESCRT-I and -III components, including Vps23, the Saccharomyces cerevisiae homologue of human Tsg101...
  19. Tong Z, Kim M, Pandey A, Espenshade P. Identification of candidate substrates for the Golgi Tul1 E3 ligase using quantitative diGly proteomics in yeast. Mol Cell Proteomics. 2014;13:2871-82 pubmed publisher
    ..This quantitative diGly proteomics methodology will serve as a robust platform for screening for stress conditions that require Tul1 E3 ligase activity. ..
  20. Hayashi M, Fukuzawa T, Sorimachi H, Maeda T. Constitutive activation of the pH-responsive Rim101 pathway in yeast mutants defective in late steps of the MVB/ESCRT pathway. Mol Cell Biol. 2005;25:9478-90 pubmed
    ..Based on extensive epistasis analysis between pathway-activating and -inactivating mutations, a model for architecture and regulation of the Rim101 pathway is proposed. ..
  21. Herrador A, Leon S, Haguenauer Tsapis R, Vincent O. A mechanism for protein monoubiquitination dependent on a trans-acting ubiquitin-binding domain. J Biol Chem. 2013;288:16206-11 pubmed publisher
    ..This also requires Vps23, a protein that displays an ubiquitin-E2 variant (UEV) domain...
  22. Morvan J, Rinaldi B, Friant S. Pkh1/2-dependent phosphorylation of Vps27 regulates ESCRT-I recruitment to endosomes. Mol Biol Cell. 2012;23:4054-64 pubmed publisher
    ..We propose that Vps27 phosphorylation by Pkh1/2 kinases regulates the coordinated cascade of ESCRT complex recruitment at the endosomal membrane. ..
  23. Hayden J, Williams M, Granich A, Ahn H, Tenay B, Lukehart J, et al. Vps1 in the late endosome-to-vacuole traffic. J Biosci. 2013;38:73-83 pubmed
    ..Together, we propose that Vps1 is required for correct and efficient trafficking from the late endosomal compartments to the vacuole. ..
  24. Peters T, Miller A, Tourette C, Agren H, Hubbard A, Hughes R. Genomic Analysis of ATP Efflux in Saccharomyces cerevisiae. G3 (Bethesda). 2015;6:161-70 pubmed publisher
    ..These results will facilitate analysis of ATP efflux mechanisms in higher eukaryotes. ..