Gene Symbol: STE50
Description: Ste50p
Alias: Ste50p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Xu G, Jansen G, Thomas D, Hollenberg C, Ramezani Rad M. Ste50p sustains mating pheromone-induced signal transduction in the yeast Saccharomyces cerevisiae. Mol Microbiol. 1996;20:773-83 pubmed
    ..In this study, we present a functional characterization of Ste50p, a protein that is required to sustain the pheromone-induced signal which leads cells to hormone-induced ..
  2. Grimshaw S, Mott H, Stott K, Nielsen P, Evetts K, Hopkins L, et al. Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM. J Biol Chem. 2004;279:2192-201 pubmed
    ..Here, we present the solution structure of the SAM domain of the Saccharomyces cerevisiae protein, Ste50p. Ste50p functions as a modulator of the mitogen-activated protein kinase (MAPK) cascades in S...
  3. Hao N, Zeng Y, Elston T, Dohlman H. Control of MAPK specificity by feedback phosphorylation of shared adaptor protein Ste50. J Biol Chem. 2008;283:33798-802 pubmed publisher
    ..In yeast, the adaptor protein Ste50 functions in multiple mitogen-activated protein (MAP) kinase pathways, each with unique dynamical and ..
  4. O Rourke S, Herskowitz I. The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev. 1998;12:2874-86 pubmed
    ..This cross talk required the osmosensor Sho1p, as well as Ste20p, Ste50p, the pheromone response MAPK cascade (Ste11p, Ste7p, and Fus3p or Kss1p), and Ste12p but not Ste4p or the MAPK ..
  5. Yamamoto K, Tatebayashi K, Tanaka K, Saito H. Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor. Mol Cell. 2010;40:87-98 pubmed publisher
    ..MAPK) pathway and the SHO1 branch of the osmoregulatory HOG MAPK pathway, and is mediated by binding of the Ste50 scaffold protein to the Opy2 membrane anchor...
  6. Ramezani Rad M, Jansen G, Buhring F, Hollenberg C. Ste50p is involved in regulating filamentous growth in the yeast Saccharomyces cerevisiae and associates with Ste11p. Mol Gen Genet. 1998;259:29-38 pubmed
    STE50 is required to sustain pheromone-induced signal transduction in S. cerevisiae. Here we report that Ste50p is involved in regulating pseudohyphal development. Both of these processes are also dependent on Ste11p...
  7. Bhattacharjya S, Xu P, Gingras R, Shaykhutdinov R, Wu C, Whiteway M, et al. Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex. J Mol Biol. 2004;344:1071-87 pubmed
    Ste11, a homologue of mammalian MAPKKKs, together with its binding partner Ste50 works in a number of MAPK signaling pathways of Saccharomyces cerevisiae...
  8. Bhattacharjya S, Xu P, Chakrapani M, Johnston L, Ni F. Polymerization of the SAM domain of MAPKKK Ste11 from the budding yeast: implications for efficient signaling through the MAPK cascades. Protein Sci. 2005;14:828-35 pubmed
    ..Very interestingly, the interactions of the Ste11 and Ste50 SAM domains also lead to the formation of non-homogeneous hetero-complexes with significant populations of high ..
  9. Truckses D, Bloomekatz J, Thorner J. The RA domain of Ste50 adaptor protein is required for delivery of Ste11 to the plasma membrane in the filamentous growth signaling pathway of the yeast Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:912-28 pubmed
    ..b>Ste50 protein associates constitutively via an N-terminal sterile-alpha motif domain with Ste11, and this interaction is ..

More Information


  1. Kwan J, Warner N, Pawson T, Donaldson L. The solution structure of the S.cerevisiae Ste11 MAPKKK SAM domain and its partnership with Ste50. J Mol Biol. 2004;342:681-93 pubmed
    ..facilitated by a direct interaction between the SAM domain of Ste11 with the SAM domain of its regulatory partner, Ste50. We have solved the NMR structure of the Ste11 SAM domain (PDB 1OW5), which reveals a compact, five alpha-helix ..
  2. Kwan J, Warner N, Maini J, Chan Tung K, Zakaria H, Pawson T, et al. Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction. J Mol Biol. 2006;356:142-54 pubmed
    ..alpha motif (SAM) domains of the Ste11 mitogen-activated protein kinase kinase kinase (MAPKKK) and its regulator Ste50. Previously, we solved the NMR structure of the SAM domain from Ste11 and identified two mutants that diminished ..
  3. Tatebayashi K, Yamamoto K, Tanaka K, Tomida T, Maruoka T, Kasukawa E, et al. Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway. EMBO J. 2006;25:3033-44 pubmed
    ..gain-of-function and loss-of-function alleles in four key genes involved in the SHO1 branch, namely SHO1, CDC42, STE50, and STE11. These mutants were characterized using an HOG-dependent reporter gene, 8xCRE-lacZ...
  4. Posas F, Witten E, Saito H. Requirement of STE50 for osmostress-induced activation of the STE11 mitogen-activated protein kinase kinase kinase in the high-osmolarity glycerol response pathway. Mol Cell Biol. 1998;18:5788-96 pubmed
    ..We found that strains with mutations in the STE50 gene, in combination with ssk2Delta ssk22Delta mutations, were unable to induce HOG1 phosphorylation after osmotic ..
  5. Wu C, Jansen G, Zhang J, Thomas D, Whiteway M. Adaptor protein Ste50p links the Ste11p MEKK to the HOG pathway through plasma membrane association. Genes Dev. 2006;20:734-46 pubmed
    In a variety of yeast cellular pathways, the Ste50p protein regulates the kinase function of the mitogen extracellular signal-regulated kinase kinase (MEKK) Ste11p...
  6. Barr M, Tu H, Van Aelst L, Wigler M. Identification of Ste4 as a potential regulator of Byr2 in the sexual response pathway of Schizosaccharomyces pombe. Mol Cell Biol. 1996;16:5597-603 pubmed
    ..Ste4 contains a leucine zipper and is capable of homotypic interaction. Ste4 has regions of homology with STE50, an S. cerevisiae protein required for sexual differentiation that we show can bind to STE11.
  7. Reiser V, Salah S, Ammerer G. Polarized localization of yeast Pbs2 depends on osmostress, the membrane protein Sho1 and Cdc42. Nat Cell Biol. 2000;2:620-7 pubmed
    ..Sho1 itself accumulates at sites of polar growth, but independently of stress conditions and Cdc42. These observations allow us to define the sequence of events that occurs during propogation of osmostress signals. ..
  8. Wu C, Leberer E, Thomas D, Whiteway M. Functional characterization of the interaction of Ste50p with Ste11p MAPKKK in Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:2425-40 pubmed
    ..One of the Ste11p regulators is Ste50p, and Ste11p and Ste50p associate through their respective N-terminal domains...
  9. Shock T, Thompson J, Yates J, Madhani H. Hog1 mitogen-activated protein kinase (MAPK) interrupts signal transduction between the Kss1 MAPK and the Tec1 transcription factor to maintain pathway specificity. Eukaryot Cell. 2009;8:606-16 pubmed publisher
    ..It also does not require the consensus MAPK sites of the Ste11 activator Ste50, in contrast to a recent proposal for a role for negative feedback in specificity...
  10. Jansen G, Buhring F, Hollenberg C, Ramezani Rad M. Mutations in the SAM domain of STE50 differentially influence the MAPK-mediated pathways for mating, filamentous growth and osmotolerance in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;265:102-17 pubmed
    ..All three pathways are also dependent on Ste50p. Ste50p and Ste11p interact constitutively via their N-terminal regions, which include putative SAM domains...
  11. O Rourke S, Herskowitz I. A third osmosensing branch in Saccharomyces cerevisiae requires the Msb2 protein and functions in parallel with the Sho1 branch. Mol Cell Biol. 2002;22:4739-49 pubmed
    ..These observations indicate that Msb2 is partially redundant with the Sho1 osmosensing branch for the activation of Ste11. ..
  12. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
  13. Winkler A, Arkind C, Mattison C, Burkholder A, Knoche K, Ota I. Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway, and protein tyrosine phosphatases are essential under heat stress. Eukaryot Cell. 2002;1:163-73 pubmed
    ..Second, they block inappropriate cross talk between the HOG and the cell wall integrity MAPK pathways, suggesting that PTPs are important for maintaining specificity in MAPK signaling pathways. ..
  14. Ekiel I, Sulea T, Jansen G, Kowalik M, Minailiuc O, Cheng J, et al. Binding the atypical RA domain of Ste50p to the unfolded Opy2p cytoplasmic tail is essential for the high-osmolarity glycerol pathway. Mol Biol Cell. 2009;20:5117-26 pubmed publisher
    ..HOG) pathway for osmoregulation in the yeast Saccharomyces cerevisiae involves interaction of the adaptor Ste50p with the cytoplasmic tail of single-transmembrane protein Opy2p...
  15. Slaughter B, Unruh J, Li R. Fluorescence fluctuation spectroscopy and imaging methods for examination of dynamic protein interactions in yeast. Methods Mol Biol. 2011;759:283-306 pubmed publisher
    ..In contrast, live cell fluorescence studies such as those outlined below are able to provide quantitative information on the strength, nature, timing, and location of homotypic and heterotypic protein interactions. ..
  16. Drogen F, O Rourke S, Stucke V, Jaquenoud M, Neiman A, Peter M. Phosphorylation of the MEKK Ste11p by the PAK-like kinase Ste20p is required for MAP kinase signaling in vivo. Curr Biol. 2000;10:630-9 pubmed
    ..This mechanism may serve as a paradigm for the activation of mammalian MEKKs. ..
  17. Rad M, Xu G, Hollenberg C. STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae. Mol Gen Genet. 1992;236:145-54 pubmed
    A new gene, STE50, which plays an essential role in cell differentiation in Saccharomyces cerevisiae was detected and analysed...
  18. Gallego O, Specht T, Brach T, Kumar A, Gavin A, Kaksonen M. Detection and characterization of protein interactions in vivo by a simple live-cell imaging method. PLoS ONE. 2013;8:e62195 pubmed publisher
    ..PICT to analyze protein-protein interactions from three biological pathways in the yeast Saccharomyces cerevisiae: Mitogen-activated protein kinase cascade (Ste5-Ste11-Ste50), exocytosis (exocyst complex) and endocytosis (Ede1-Syp1).
  19. Slaughter B, Huff J, Wiegraebe W, Schwartz J, Li R. SAM domain-based protein oligomerization observed by live-cell fluorescence fluctuation spectroscopy. PLoS ONE. 2008;3:e1931 pubmed publisher
    ..and binding stoichiometry of high-order, multi-component complexes of (SAM) domain proteins Ste11 and Ste50 in live yeast cells using fluorescence fluctuation methods...
  20. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..Thus, the reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions. ..
  21. Geyer C, Colman Lerner A, Brent R. "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections. Proc Natl Acad Sci U S A. 1999;96:8567-72 pubmed
    ..Forward genetic analysis with peptide aptamer "mutagens" should be particularly useful in elucidating genetic networks in organisms and processes for which classical genetics is not feasible. ..
  22. Tatebayashi K, Yamamoto K, Nagoya M, Takayama T, Nishimura A, Sakurai M, et al. Osmosensing and scaffolding functions of the oligomeric four-transmembrane domain osmosensor Sho1. Nat Commun. 2015;6:6975 pubmed publisher
    ..osmolarity induces structural changes in the Sho1 TM domains and Sho1 binding to the cytoplasmic adaptor protein Ste50, which leads to Hog1 activation. Besides its osmosensing function, the Sho1 oligomer serves as a scaffold...
  23. Shen Z, Li Y, Gasparski A, Abeliovich H, Greenberg M. Cardiolipin Regulates Mitophagy through the Protein Kinase C Pathway. J Biol Chem. 2017;292:2916-2923 pubmed publisher
    ..Deletion of HOG pathway genes SHO1, SSK1, STE50, and HOG1 exacerbated crd1Δ growth. 1 m sorbitol and 0...
  24. Wassmann K, Ammerer G. Overexpression of the G1-cyclin gene CLN2 represses the mating pathway in Saccharomyces cerevisiae at the level of the MEKK Ste11. J Biol Chem. 1997;272:13180-8 pubmed
    ..G protein-independent activation of Ste11 caused by an rga1 pbs2 mutation is resistant to high levels of Cln2 kinase. Therefore our results suggest that Cln2-dependent repression of the mating pathway occurs at the level of Ste11. ..
  25. Zhou J, Zhong Q, Li G, Greenberg M. Loss of cardiolipin leads to longevity defects that are alleviated by alterations in stress response signaling. J Biol Chem. 2009;284:18106-14 pubmed publisher
    ..These findings show for the first time that perturbation of CL synthesis leads to decreased longevity in yeast, which is restored by altering signaling through stress response pathways. ..
  26. Bhunia A, Domadia P, Mohanram H, Bhattacharjya S. NMR structural studies of the Ste11 SAM domain in the dodecyl phosphocholine micelle. Proteins. 2009;74:328-43 pubmed publisher
    ..well-folded dimeric structure that is involved in interaction with the cognate SAM domain from an adaptor protein Ste50. In this work, we show that the Ste11 SAM domain has an intrinsic affinity towards the lipid membranes...
  27. Poplinski A, Hopp C, Ramezani Rad M. Ste50 adaptor protein influences Ras/cAMP-driven stress-response and cell survival in Saccharomyces cerevisiae. Curr Genet. 2007;51:257-68 pubmed
    The Ste50 adaptor protein is involved in a variety of cellular pathways that yeast cells use to adapt rapidly to environmental changes...
  28. Annan R, Wu C, Waller D, Whiteway M, Thomas D. Rho5p is involved in mediating the osmotic stress response in Saccharomyces cerevisiae, and its activity is regulated via Msi1p and Npr1p by phosphorylation and ubiquitination. Eukaryot Cell. 2008;7:1441-9 pubmed publisher
    ..Rho5p binds to Ste50p, and the expression of the activated RHO5(Q91H) allele in an Deltaste50 strain is lethal under conditions of ..
  29. Caponigro G, Abedi M, Hurlburt A, Maxfield A, Judd W, Kamb A. Transdominant genetic analysis of a growth control pathway. Proc Natl Acad Sci U S A. 1998;95:7508-13 pubmed
    ..V., Kazarov, A. R., Thimmapaya, R., Axenovich, S. A., Mazo, I. A. & Roninson, I. B. (1994) Proc. Natl. Acad. Sci. USA 91, 3744-3748], suggest that transdominant genetic analysis of the type described here will be broadly applicable. ..
  30. Wu C, Arcand M, Jansen G, Zhong M, Iouk T, Thomas D, et al. Phosphorylation of the MAPKKK regulator Ste50p in Saccharomyces cerevisiae: a casein kinase I phosphorylation site is required for proper mating function. Eukaryot Cell. 2003;2:949-61 pubmed
    ..Ste11p is a member of the MAP3K (or MEKK) family, which is conserved from yeast to mammals. Ste50p is involved in all the signaling pathways that require Ste11p function, yet little is known about the regulation ..
  31. Karunanithi S, Cullen P. The filamentous growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 transcriptional repressors in Saccharomyces cerevisiae. Genetics. 2012;192:869-87 pubmed publisher
    ..Taken together, our findings provide the first regulatory link in yeast between components of the AMPK pathway and a MAPK pathway that controls cellular differentiation. ..
  32. Nagiec M, Dohlman H. Checkpoints in a yeast differentiation pathway coordinate signaling during hyperosmotic stress. PLoS Genet. 2012;8:e1002437 pubmed publisher
    ..Third, stress promotes the phosphorylation of a shared pathway component, Ste50, and thereby dampens pheromone-induced MAPK activation...