Gene Symbol: STE5
Description: Ste5p
Alias: HMD3, NUL3, Ste5p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Wang Y, Chen W, Simpson D, Elion E. Cdc24 regulates nuclear shuttling and recruitment of the Ste5 scaffold to a heterotrimeric G protein in Saccharomyces cerevisiae. J Biol Chem. 2005;280:13084-96 pubmed
    ..Here, we show that Cdc24 promotes MAPK signaling during mating through interactions with Ste5, a scaffold that must shuttle through the nucleus and bind to the beta subunit (Ste4) of a G protein for Ste20 to ..
  2. Winters M, Lamson R, Nakanishi H, Neiman A, Pryciak P. A membrane binding domain in the ste5 scaffold synergizes with gbetagamma binding to control localization and signaling in pheromone response. Mol Cell. 2005;20:21-32 pubmed
    ..of mitogen-activated protein (MAP) kinase cascade signaling by yeast mating pheromones involves recruitment of the Ste5 scaffold protein to the plasma membrane by the receptor-activated Gbetagamma dimer...
  3. Whiteway M, Wu C, Leeuw T, Clark K, Fourest Lieuvin A, Thomas D, et al. Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p. Science. 1995;269:1572-5 pubmed
    ..An amino-terminal fragment of the MAP kinase scaffold protein Ste5p that interfered with pheromone-induced cell cycle arrest was identified...
  4. Inouye C, Dhillon N, Durfee T, Zambryski P, Thorner J. Mutational analysis of STE5 in the yeast Saccharomyces cerevisiae: application of a differential interaction trap assay for examining protein-protein interactions. Genetics. 1997;147:479-92 pubmed
    b>Ste5 is essential for the yeast mating pheromone response pathway and is thought to function as a scaffold that organizes the components of the mitogen-activated protein kinase (MAPK) cascade...
  5. Leeuw T, Wu C, Schrag J, Whiteway M, Thomas D, Leberer E. Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases. Nature. 1998;391:191-5 pubmed
  6. Wang Y, Elion E. Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components. Mol Biol Cell. 2003;14:2543-58 pubmed
    The Ste5 scaffold activates an associated mitogen-activated protein kinase cascade by binding through its RING-H2 domain to a Gbetagamma dimer (Ste4/Ste18) at the plasma membrane in a recruitment event that requires prior nuclear ..
  7. Printen J, Sprague G. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics. 1994;138:609-19 pubmed
    ..Pathway components Ste4p, Ste5p, Ste7p, Ste11p, Ste12p, Ste20p, Fus3p and Kss1p were tested in all pairwise combinations...
  8. Marcus S, Polverino A, Barr M, Wigler M. Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc Natl Acad Sci U S A. 1994;91:7762-6 pubmed
    We present genetic evidence for complex formation of STE5 and the STE11, STE7, and FUS3 protein kinases, the pheromone-responsive mitogen-activated protein kinase module of Saccharomyces cerevisiae...
  9. Garrenton L, Young S, Thorner J. Function of the MAPK scaffold protein, Ste5, requires a cryptic PH domain. Genes Dev. 2006;20:1946-58 pubmed
    b>Ste5, the prototypic mitogen-activated protein kinase (MAPK) scaffold protein, associates with plasma membrane-tethered Gbetagamma freed upon pheromone receptor occupancy, thereby initiating downstream signaling...

More Information


  1. Choi K, Satterberg B, Lyons D, Elion E. Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell. 1994;78:499-512 pubmed
    b>Ste5 is a Zn2+ finger-like protein thought to function before three kinases, Ste11 (a MEKK), Ste7 (a MEK), and Fus3 (a MAPK), in a conserved MAP kinase cascade required for mating in S. cerevisiae...
  2. Pryciak P, Huntress F. Membrane recruitment of the kinase cascade scaffold protein Ste5 by the Gbetagamma complex underlies activation of the yeast pheromone response pathway. Genes Dev. 1998;12:2684-97 pubmed
    ..We also show that pheromone stimulates translocation of the kinase cascade scaffold protein Ste5 to the cell surface...
  3. Maleri S, Ge Q, Hackett E, Wang Y, Dohlman H, Errede B. Persistent activation by constitutive Ste7 promotes Kss1-mediated invasive growth but fails to support Fus3-dependent mating in yeast. Mol Cell Biol. 2004;24:9221-38 pubmed
    ..The mating pathway also requires the scaffold-Ste5 and the additional MAPK-Fus3...
  4. Malleshaiah M, Shahrezaei V, Swain P, Michnick S. The scaffold protein Ste5 directly controls a switch-like mating decision in yeast. Nature. 2010;465:101-5 pubmed publisher
    ..the MAPK Fus3 and a phosphatase Ptc1 for control of the phosphorylation state of four sites on the scaffold protein Ste5. This competition results in a switch-like dissociation of Fus3 from Ste5 that is necessary to generate the switch-..
  5. Choi Y, Kim S, Park K, Choi K. Differential transmission of G1 cell cycle arrest and mating signals by Saccharomyces cerevisiae Ste5 mutants in the pheromone pathway. Biochem Cell Biol. 1999;77:459-68 pubmed
    Saccharomyces cerevisiae Ste5 is a scaffold protein that recruits many pheromone signaling molecules to sequester the pheromone pathway from other homologous mitogen-activated protein kinase pathways...
  6. Jenness D, Goldman B, Hartwell L. Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression. Mol Cell Biol. 1987;7:1311-9 pubmed
    ..Sterility of the ste5-3 mutant was suppressed by mutation ros1-1 but not by sst2-1...
  7. Pope P, Bhaduri S, Pryciak P. Regulation of cyclin-substrate docking by a G1 arrest signaling pathway and the Cdk inhibitor Far1. Curr Biol. 2014;24:1390-1396 pubmed publisher
    ..Our findings uncover a novel mechanism of Cdk regulation by external signals and shed new light on Far1 function to provide a revised view of cell-cycle arrest in this model system. ..
  8. van Drogen F, Stucke V, Jorritsma G, Peter M. MAP kinase dynamics in response to pheromones in budding yeast. Nat Cell Biol. 2001;3:1051-9 pubmed
    ..This MAPK module interacts with the scaffold molecule Ste5p. Here we show that Ste11p and Ste7p were predominantly cytoplasmic proteins, while Ste5p and Fus3p were found in ..
  9. Sette C, Inouye C, Stroschein S, Iaquinta P, Thorner J. Mutational analysis suggests that activation of the yeast pheromone response mitogen-activated protein kinase pathway involves conformational changes in the Ste5 scaffold protein. Mol Biol Cell. 2000;11:4033-49 pubmed
    b>Ste5 is essential for pheromone response and binds components of a mitogen-activated protein kinase (MAPK) cascade: Ste11 (MEKK), Ste7 (MEK), and Fus3 (MAPK)...
  10. Park S, Zarrinpar A, Lim W. Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms. Science. 2003;299:1061-4 pubmed they precisely orient and activate them? We found that the yeast mitogen-activated protein (MAP) kinase scaffold Ste5 is tolerant to major stereochemical perturbations; heterologous protein interactions could functionally replace ..
  11. Garrenton L, Braunwarth A, Irniger S, Hurt E, Kunzler M, Thorner J. Nucleus-specific and cell cycle-regulated degradation of mitogen-activated protein kinase scaffold protein Ste5 contributes to the control of signaling competence. Mol Cell Biol. 2009;29:582-601 pubmed publisher
    ..b>Ste5 scaffold protein is essential for pheromone response because it couples pheromone receptor stimulation to ..
  12. Lyons D, Mahanty S, Choi K, Manandhar M, Elion E. The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:4095-106 pubmed
    ..Bem1 protein stimulates Fus3 (MAPK) activity and associates with Ste5, the tethering protein essential for activation of the MAPK kinase kinase Ste11...
  13. Villasmil M, Ansbach A, Nickels J. The putative lipid transporter, Arv1, is required for activating pheromone-induced MAP kinase signaling in Saccharomyces cerevisiae. Genetics. 2011;187:455-65 pubmed publisher
    ..lack the ability to initiate pheromone-induced G1 cell cycle arrest, due to failure to polarize PI(4,5)P(2) and the Ste5 scaffold, which results in weakened MAP kinase signaling activity...
  14. Conte D, Barber E, Banerjee M, Garfinkel D, Curcio M. Posttranslational regulation of Ty1 retrotransposition by mitogen-activated protein kinase Fus3. Mol Cell Biol. 1998;18:2502-13 pubmed
    ..analyses revealed that components of the pheromone response pathway that act upstream of Fus3, including Ste4, Ste5, Ste7, and Ste11, are required for the posttranslational suppression of Ty1 transposition by Fus3...
  15. Yablonski D, Marbach I, Levitzki A. Dimerization of Ste5, a mitogen-activated protein kinase cascade scaffold protein, is required for signal transduction. Proc Natl Acad Sci U S A. 1996;93:13864-9 pubmed
    ..protein kinase cascade of the Saccharomyces cerevisiae pheromone response pathway is organized on the Ste5 protein, which binds each of the kinases of the cascade prior to signaling...
  16. Slaughter B, Unruh J, Li R. Fluorescence fluctuation spectroscopy and imaging methods for examination of dynamic protein interactions in yeast. Methods Mol Biol. 2011;759:283-306 pubmed publisher
    ..In contrast, live cell fluorescence studies such as those outlined below are able to provide quantitative information on the strength, nature, timing, and location of homotypic and heterotypic protein interactions. ..
  17. Kranz J, Satterberg B, Elion E. The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5. Genes Dev. 1994;8:313-27 pubmed
    ..MAP kinase Fus3 is thought to occur via a linear pathway involving the sequential action of three proteins: Ste5, a protein of unknown function, Ste11, a MAPKK kinase homolog, and Ste7, a MAPK kinase homolog which phosphorylates ..
  18. Caponigro G, Abedi M, Hurlburt A, Maxfield A, Judd W, Kamb A. Transdominant genetic analysis of a growth control pathway. Proc Natl Acad Sci U S A. 1998;95:7508-13 pubmed
    ..V., Kazarov, A. R., Thimmapaya, R., Axenovich, S. A., Mazo, I. A. & Roninson, I. B. (1994) Proc. Natl. Acad. Sci. USA 91, 3744-3748], suggest that transdominant genetic analysis of the type described here will be broadly applicable. ..
  19. Flotho A, Simpson D, Qi M, Elion E. Localized feedback phosphorylation of Ste5p scaffold by associated MAPK cascade. J Biol Chem. 2004;279:47391-401 pubmed
    Scaffold proteins play pivotal roles during signal transduction. In Saccharomyces cerevisiae, the Ste5p scaffold protein is required for activation of the mating MAPK cascade in response to mating pheromone and assembles a G protein-MAPK ..
  20. Inouye C, Dhillon N, Thorner J. Ste5 RING-H2 domain: role in Ste4-promoted oligomerization for yeast pheromone signaling. Science. 1997;278:103-6 pubmed
    b>Ste5 is a scaffold for the mitogen-activated protein kinase (MAPK) cascade components in a yeast pheromone response pathway...
  21. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..Thus, the reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions. ..
  22. Pryciak P, Hartwell L. AKR1 encodes a candidate effector of the G beta gamma complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction. Mol Cell Biol. 1996;16:2614-26 pubmed
  23. Sugimoto K, Matsumoto K, Kornberg R, Reed S, Wittenberg C. Dosage suppressors of the dominant G1 cyclin mutant CLN3-2: identification of a yeast gene encoding a putative RNA/ssDNA binding protein. Mol Gen Genet. 1995;248:712-8 pubmed
    ..genes (designated HMD genes) of the mating defect caused by CLN3-2, a dominant mutation in CLN3, HMD2 and HMD3 are identical to STE4 and STE5, respectively, HMD1 is an essential gene that encodes a protein containing a ..
  24. Barr M, Tu H, Van Aelst L, Wigler M. Identification of Ste4 as a potential regulator of Byr2 in the sexual response pathway of Schizosaccharomyces pombe. Mol Cell Biol. 1996;16:5597-603 pubmed
    ..Ste4 contains a leucine zipper and is capable of homotypic interaction. Ste4 has regions of homology with STE50, an S. cerevisiae protein required for sexual differentiation that we show can bind to STE11. ..
  25. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
  26. O Rourke S, Herskowitz I. The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev. 1998;12:2874-86 pubmed
    ..response MAPK cascade (Ste11p, Ste7p, and Fus3p or Kss1p), and Ste12p but not Ste4p or the MAPK scaffold protein, Ste5p. The cross talk in hog1 mutants induced multiple responses of the pheromone response pathway: induction of a FUS1::..
  27. Zalatan J, Coyle S, Rajan S, Sidhu S, Lim W. Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation. Science. 2012;337:1218-22 pubmed publisher
    ..We show that the scaffold protein Ste5, which organizes the yeast mating mitogen-activated protein kinase (MAPK) pathway, does not use sequestration to ..
  28. Bhunia A, Mohanram H, Bhattacharjya S. Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway. Biochim Biophys Acta. 2012;1818:1250-60 pubmed publisher
    In the mitogen activated protein kinase (MAPK) cascades of budding yeast, the scaffold protein Ste5 is recruited to the plasma membrane to transmit pheromone induced signal. A region or domain of Ste5 i.e...
  29. Breitkreutz A, Boucher L, Tyers M. MAPK specificity in the yeast pheromone response independent of transcriptional activation. Curr Biol. 2001;11:1266-71 pubmed
    ..physically occludes Kss1 from pheromone-activated signaling complexes, which are formed on the scaffold protein Ste5. However, we find that genome-wide expression profiles of pheromone-treated wild-type, fus3, and kss1 deletion ..
  30. Hu Z, Wang Y, Yu L, Mahanty S, Mendoza N, Elion E. Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export. Biochem Cell Biol. 2016;94:109-28 pubmed publisher
    Careful control of the available pool of the MAPK scaffold Ste5 is important for mating-pathway activation and the prevention of inappropriate mating differentiation in haploid Saccharomyces cerevisiae...
  31. Hasson M, Blinder D, Thorner J, Jenness D. Mutational activation of the STE5 gene product bypasses the requirement for G protein beta and gamma subunits in the yeast pheromone response pathway. Mol Cell Biol. 1994;14:1054-65 pubmed
    The STE5 gene encodes an essential element of the pheromone response pathway which is known to act either after the G subunit encoded by the STE4 gene or at the same step...
  32. Nelson B, Parsons A, Evangelista M, Schaefer K, Kennedy K, Ritchie S, et al. Fus1p interacts with components of the Hog1p mitogen-activated protein kinase and Cdc42p morphogenesis signaling pathways to control cell fusion during yeast mating. Genetics. 2004;166:67-77 pubmed
    ..Taken together, our results suggest that Fus1p acts as a scaffold for the assembly of a cell surface complex involved in polarized secretion of septum-degrading enzymes and inhibition of HOG pathway signaling to promote cell fusion. ..
  33. Elion E, Brill J, Fink G. FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc Natl Acad Sci U S A. 1991;88:9392-6 pubmed
    ..The diverse roles for FUS3 suggest that the FUS3 protein kinase has multiple substrates, some of which may be shared with KSS1. ..
  34. Yashar B, Irie K, Printen J, Stevenson B, Sprague G, Matsumoto K, et al. Yeast MEK-dependent signal transduction: response thresholds and parameters affecting fidelity. Mol Cell Biol. 1995;15:6545-53 pubmed to compensate for defects in the cell integrity pathway, but it does so only when it is overproduced or when Ste5p is missing...
  35. Strickfaden S, Winters M, Ben Ari G, Lamson R, Tyers M, Pryciak P. A mechanism for cell-cycle regulation of MAP kinase signaling in a yeast differentiation pathway. Cell. 2007;128:519-31 pubmed
    ..Here we show that the target of this inhibition is Ste5, the MAPK cascade scaffold protein...
  36. Louvion J, Abbas Terki T, Picard D. Hsp90 is required for pheromone signaling in yeast. Mol Biol Cell. 1998;9:3071-83 pubmed
    ..These findings further corroborate the view that molecular chaperones must also be considered as transient or stable components of signal transduction pathways. ..
  37. Bhaduri S, Valk E, Winters M, Gruessner B, Loog M, Pryciak P. A docking interface in the cyclin Cln2 promotes multi-site phosphorylation of substrates and timely cell-cycle entry. Curr Biol. 2015;25:316-25 pubmed publisher
    ..Furthermore, this docking function helps ensure full phosphorylation of substrates with multiple phosphorylation sites, and this contributes to punctual cell-cycle entry. ..
  38. Perlman R, Yablonski D, Simchen G, Levitzki A. Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants. Proc Natl Acad Sci U S A. 1993;90:5474-8 pubmed
    The STE5 gene of Saccharomyces cerevisiae was cloned using a screening procedure designed to isolate genes of the S. cerevisiae pheromone response pathway...
  39. Jansen G, Buhring F, Hollenberg C, Ramezani Rad M. Mutations in the SAM domain of STE50 differentially influence the MAPK-mediated pathways for mating, filamentous growth and osmotolerance in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;265:102-17 pubmed
    ..Thus the Ste50p-Ste11p interaction may differentially modulate the flow of information through the various MAPK-mediated pathways. ..
  40. Kusari A, Molina D, Sabbagh W, Lau C, Bardwell L. A conserved protein interaction network involving the yeast MAP kinases Fus3 and Kss1. J Cell Biol. 2004;164:267-77 pubmed
    ..CD/7m region) disrupt binding to an important subset of their binding partners, including the Ste7 MAPK kinase, the Ste5 adaptor/scaffold protein, and the Dig1 and Dig2 transcriptional repressors...
  41. Mahanty S, Wang Y, Farley F, Elion E. Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade. Cell. 1999;98:501-12 pubmed
    Localization of Ste5 to GP at the plasma membrane is essential for transmission of the pheromone signal to associated MAP kinase cascade enzymes...
  42. Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher
    ..Unlike classical pseudo-hyphal growth, sadF signaling requires Ste5, Ste4 and Ste18, the scaffold protein and G-protein β and γ subunits from the pheromone response pathway, ..
  43. Flatauer L, Zadeh S, Bardwell L. Mitogen-activated protein kinases with distinct requirements for Ste5 scaffolding influence signaling specificity in Saccharomyces cerevisiae. Mol Cell Biol. 2005;25:1793-803 pubmed
    ..The prototype scaffold Ste5 binds to multiple components of the Saccharomyces cerevisiae mating pheromone response pathway, thereby conducting ..
  44. Andersson J, Simpson D, Qi M, Wang Y, Elion E. Differential input by Ste5 scaffold and Msg5 phosphatase route a MAPK cascade to multiple outcomes. EMBO J. 2004;23:2564-76 pubmed has suggested that the Kss1 MAPK cascade is activated independently of the mating G protein (Ste4)-scaffold (Ste5) system during IG...
  45. Yerko V, Sulea T, Ekiel I, Harcus D, Baardsnes J, Cygler M, et al. Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling. Mol Biol Cell. 2013;24:409-20 pubmed publisher
    The Ste5 protein forms a scaffold that associates and regulates the components of the mitogen-activated protein (MAP) kinase cascade that controls mating-pheromone-mediated signaling in the yeast Saccharomyces cerevisiae...
  46. Shuster J. Mating-defective ste mutations are suppressed by cell division cycle start mutations in Saccharomyces cerevisiae. Mol Cell Biol. 1982;2:1052-63 pubmed
    ..A class of mutants (carrying ste4, ste5, ste7, ste11, or ste12) which is insensitive to mating pheromone and sterile has also been described...
  47. Nagiec M, McCarter P, Kelley J, Dixit G, Elston T, Dohlman H. Signal inhibition by a dynamically regulated pool of monophosphorylated MAPK. Mol Biol Cell. 2015;26:3359-71 pubmed publisher
    ..cells lacking the dual-specificity phosphatase (msg5Δ) or that are deficient in docking to the MAPK-scaffold (Ste5(ND)) accumulate a greater proportion of dual-phosphorylated Fus3...
  48. Nakayama N, Kaziro Y, Arai K, Matsumoto K. Role of STE genes in the mating factor signaling pathway mediated by GPA1 in Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:3777-83 pubmed
    The ste mutants (ste2, ste4, ste5, ste7, ste11, and ste12) are insensitive to mating factors and are, therefore, sterile...
  49. Coyle S, Flores J, Lim W. Exploitation of latent allostery enables the evolution of new modes of MAP kinase regulation. Cell. 2013;154:875-87 pubmed publisher
    ..Here, we trace the evolution of two allosteric activator motifs within the yeast scaffold protein Ste5 that specifically target the mating MAP kinase Fus3...
  50. Niu W, Li Z, Zhan W, Iyer V, Marcotte E. Mechanisms of cell cycle control revealed by a systematic and quantitative overexpression screen in S. cerevisiae. PLoS Genet. 2008;4:e1000120 pubmed publisher
    ..This work thus implicates new genes in cell cycle progression, complements previous screens, and lays the foundation for future experiments to define more precisely roles for these genes in cell cycle progression. ..
  51. Geyer C, Colman Lerner A, Brent R. "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections. Proc Natl Acad Sci U S A. 1999;96:8567-72 pubmed
    ..Forward genetic analysis with peptide aptamer "mutagens" should be particularly useful in elucidating genetic networks in organisms and processes for which classical genetics is not feasible. ..
  52. Feng Y, Song L, Kincaid E, Mahanty S, Elion E. Functional binding between Gbeta and the LIM domain of Ste5 is required to activate the MEKK Ste11. Curr Biol. 1998;8:267-78 pubmed
    ..The pathway requires Ste5, a scaffold protein that tethers the MAP kinase cascade enzymes into a high molecular weight complex...
  53. Schrick K, Garvik B, Hartwell L. Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone. Genetics. 1997;147:19-32 pubmed
    ..Cells mutant for components of the mitogen-activated protein (MAP) kinase cascade (ste5, ste20, ste11, ste7 or fus3 kss1) formed diploids at a frequency 1% that of the wild-type control, but formed ..
  54. Maeder C, Hink M, Kinkhabwala A, Mayr R, Bastiaens P, Knop M. Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol. 2007;9:1319-26 pubmed
    ..quantified the abundance of complexes in the cytoplasm among the MAPKs Ste11, Ste7, Fus3 and the scaffold protein Ste5 in yeast pheromone signalling using fluorescence cross-correlation spectroscopy (FCCS)...
  55. Hao N, Nayak S, Behar M, Shanks R, Nagiec M, Errede B, et al. Regulation of cell signaling dynamics by the protein kinase-scaffold Ste5. Mol Cell. 2008;30:649-56 pubmed publisher
    ..Fus3 activity requires the scaffold protein Ste5; when binding to Ste5 is abrogated, Fus3 behaves like Kss1, and the cells no longer respond to a gradient or mate ..
  56. Errede B, Vered L, Ford E, Peña M, Elston T. Pheromone-induced morphogenesis and gradient tracking are dependent on the MAPK Fus3 binding to Gα. Mol Biol Cell. 2015;26:3343-58 pubmed publisher
  57. Akada R, Kallal L, Johnson D, Kurjan J. Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics. 1996;143:103-17 pubmed
    ..Overexpression of Ste5p under galactose control activated the pheromone response pathway...
  58. Good M, Tang G, Singleton J, Remenyi A, Lim W. The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation. Cell. 2009;136:1085-97 pubmed publisher
    The scaffold protein Ste5 is required to properly direct signaling through the yeast mating pathway to the mitogen-activated protein kinase (MAPK), Fus3. Scaffolds are thought to function by tethering kinase and substrate in proximity...
  59. Bhaduri S, Pryciak P. Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes. Curr Biol. 2011;21:1615-23 pubmed publisher
    ..These early Cdk substrates include signaling proteins in the pheromone response pathway. Two such proteins, Ste5 and Ste20, are phosphorylated only when Cdk is associated with the G1/S cyclins Cln1 and Cln2 and not G1, S, or M ..
  60. Qi M, Elion E. Formin-induced actin cables are required for polarized recruitment of the Ste5 scaffold and high level activation of MAPK Fus3. J Cell Sci. 2005;118:2837-48 pubmed
    ..In budding yeast, the Ste5 scaffold is recruited to a receptor-coupled G protein during mating pheromone stimulation, allowing the tethered ..
  61. Stevenson B, Rhodes N, Errede B, Sprague G. Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein. Genes Dev. 1992;6:1293-304 pubmed
    ..Each of these suppressor mutations suppresses ste4 and ste5 deletions but not deletions in STE7, STE11, or STE12...
  62. Won A, Garbarino J, Lim W. Recruitment interactions can override catalytic interactions in determining the functional identity of a protein kinase. Proc Natl Acad Sci U S A. 2011;108:9809-14 pubmed publisher
  63. Kim J, Lee C, Rath A, Davidson A. Recognition of non-canonical peptides by the yeast Fus1p SH3 domain: elucidation of a common mechanism for diverse SH3 domain specificities. J Mol Biol. 2008;377:889-901 pubmed publisher
    ..peptides derived from two putative in vivo binding partners from yeast proteins, Bnr1p and Ste5p, with K(d) values in the low micromolar range...
  64. Strickfaden S, Pryciak P. Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein. Mol Biol Cell. 2008;19:181-97 pubmed
    ..These findings raise the possibility that the Galphabetagamma heterotrimer can function in a partially dissociated state, tethered by the N-terminal interface. ..
  65. Slaughter B, Schwartz J, Li R. Mapping dynamic protein interactions in MAP kinase signaling using live-cell fluorescence fluctuation spectroscopy and imaging. Proc Natl Acad Sci U S A. 2007;104:20320-5 pubmed
    ..pairs observed the interactions of Ste7 (MAPK kinase) with the MAPKs, Fus3 or Kss1, and of the scaffold protein, Ste5, with Ste7 and Ste11 (MAPK kinase kinase) in the cytosol, providing in vivo constants of their binding equilibrium...
  66. Leberer E, Dignard D, Harcus D, Hougan L, Whiteway M, Thomas D. Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1. Mol Gen Genet. 1993;241:241-54 pubmed
    ..This screen identified the STE5 gene encoding an essential component of the pheromone signal transduction pathway...
  67. Yu Y, Hirsch J. An essential gene pair in Saccharomyces cerevisiae with a potential role in mating. DNA Cell Biol. 1995;14:411-8 pubmed
    ..Overexpression of STE5, STE18 (which encodes the G gamma subunit), and a previously unidentified gene, termed SSF1, partially suppressed ..