STE20

Summary

Gene Symbol: STE20
Description: mitogen-activated protein kinase kinase kinase kinase STE20
Alias: mitogen-activated protein kinase kinase kinase kinase STE20
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Holly S, Blumer K. PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae. J Cell Biol. 1999;147:845-56 pubmed
    ..Inactivation of the PAK homologues Ste20 and Cla4 at various points in the cell cycle resulted in loss of cell and actin cytoskeletal polarity, but not in ..
  2. Roberts R, Fink G. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev. 1994;8:2974-85 pubmed
    ..We find that the same components of the MAP kinase cascade necessary for diploid pseudohyphal development (STE20, STE11, STE7, and STE12) are also required for both filament formation and agar penetration in haploids...
  3. Höfken T, Schiebel E. A role for cell polarity proteins in mitotic exit. EMBO J. 2002;21:4851-62 pubmed
    ..Moreover, Cdc24, Cdc42 and Ste20, another PAK, probably function parallel to Lte1 in facilitating mitotic exit...
  4. Wu C, Whiteway M, Thomas D, Leberer E. Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. J Biol Chem. 1995;270:15984-92 pubmed
  5. Lee B, Elion E. The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components. Proc Natl Acad Sci U S A. 1999;96:12679-84 pubmed
    ..and invasive growth (IG) pathways use the same mitogen-activated protein kinase kinase kinase kinase (MAPKKKK, Ste20), MAPKKK (Ste11), MAPKK (Ste7), and transcription factor (Ste12) to promote either G(1) arrest and fusion or ..
  6. Ramer S, Davis R. A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993;90:452-6 pubmed
    This work reports the identification, characterization, and nucleotide sequence of STE20, a newly discovered gene involved in the Saccharomyces cerevisiae mating response pathway, to date one of the best understood signal transduction ..
  7. Eby J, Holly S, van Drogen F, Grishin A, Peter M, Drubin D, et al. Actin cytoskeleton organization regulated by the PAK family of protein kinases. Curr Biol. 1998;8:967-70 pubmed
    ..Here, we show that mutants of the budding yeast Saccharomyces cerevisiae lacking the PAK homologs Ste20 and Cla4 exhibit actin cytoskeletal defects, in vivo and in vitro, that resemble those of cdc42-1 mutants...
  8. Leeuw T, Fourest Lieuvin A, Wu C, Chenevert J, Clark K, Whiteway M, et al. Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin. Science. 1995;270:1210-3 pubmed
    ..Thus, the association of Bem1p with Ste20p and Ste5p may contribute to the conveyance of spatial information that regulates polarized rearrangement of the actin cytoskeleton during yeast mating. ..
  9. Leberer E, Dignard D, Harcus D, Thomas D, Whiteway M. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. EMBO J. 1992;11:4815-24 pubmed
    ..We have identified a new gene, designated STE20, which encodes a protein kinase homologue with sequence similarity to protein kinase C, which is required to ..

More Information

Publications81

  1. Kozubowski L, Saito K, Johnson J, Howell A, Zyla T, Lew D. Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex. Curr Biol. 2008;18:1719-26 pubmed publisher
    ..Our findings provide mechanistic insight into an evolutionarily conserved pattern-forming positive-feedback pathway. ..
  2. Höfken T, Schiebel E. Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2. J Cell Biol. 2004;164:219-31 pubmed
    The guanine nucleotide exchange factor Cdc24, the GTPase Cdc42, and the Cdc42 effectors Cla4 and Ste20, two p21-activated kinases, form a signal transduction cascade that promotes mitotic exit in yeast...
  3. Ahn S, Cheung W, Hsu J, Diaz R, Smith M, Allis C. Sterile 20 kinase phosphorylates histone H2B at serine 10 during hydrogen peroxide-induced apoptosis in S. cerevisiae. Cell. 2005;120:25-36 pubmed
    ..b>Ste20 kinase, a yeast homolog of mammalian Mst1 kinase, translocates into the nucleus in a caspase-independent fashion ..
  4. Raitt D, Posas F, Saito H. Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway. EMBO J. 2000;19:4623-31 pubmed
    ..The yeast PAK homolog Ste20 is essential for the Sho1-dependent activation of the Hog1 MAP kinase in response to severe osmotic stress...
  5. Ni L, Snyder M. A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell. 2001;12:2147-70 pubmed
    ..Genome-wide screens of defined collections of mutants hold significant promise for dissecting many biological processes in yeast. ..
  6. Wu C, Lytvyn V, Thomas D, Leberer E. The phosphorylation site for Ste20p-like protein kinases is essential for the function of myosin-I in yeast. J Biol Chem. 1997;272:30623-6 pubmed
    ..Our results suggest that phosphorylation of the head domain by Ste20p-like protein kinases plays an essential role in the function of myosin-I in yeast cells. ..
  7. Meitinger F, Richter H, Heisel S, Hub B, Seufert W, Pereira G. A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity. PLoS Biol. 2013;11:e1001495 pubmed publisher
    ..Our findings provide unexpected insights into the temporal control of cytokinesis and describe the importance of a Gps1-dependent mechanism for highly accurate polarity switching between two closely connected locations. ..
  8. Cvrckova F, De Virgilio C, Manser E, Pringle J, Nasmyth K. Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast. Genes Dev. 1995;9:1817-30 pubmed
    The yeast Ste20 protein kinase is involved in pheromone response. Mammalian homologs of Ste20 exist, but their function remains unknown...
  9. Harkins A, London S, Dolan J. An upstream regulator and downstream target of phospholipase D1 activity during pheromone response in Saccharomyces cerevisiae. FEMS Yeast Res. 2008;8:237-44 pubmed
    ..Finally, kinase activity assays provided evidence for the stimulation of Ste20p by PA. These findings highlight the important interactions among PLD1, Cdc42p and Ste20p during PCG in S. cerevisiae. ..
  10. Ash J, Wu C, Larocque R, Jamal M, Stevens W, Osborne M, et al. Genetic analysis of the interface between Cdc42p and the CRIB domain of Ste20p in Saccharomyces cerevisiae. Genetics. 2003;163:9-20 pubmed
    ..These results suggest that the Ste20p-Cdc42p interaction plays a direct role in Ste20p kinase function and that this interaction is required for efficient activity of the pheromone response pathway. ..
  11. Gorelik M, Stanger K, Davidson A. A Conserved residue in the yeast Bem1p SH3 domain maintains the high level of binding specificity required for function. J Biol Chem. 2011;286:19470-7 pubmed publisher
  12. Annan R, Wu C, Waller D, Whiteway M, Thomas D. Rho5p is involved in mediating the osmotic stress response in Saccharomyces cerevisiae, and its activity is regulated via Msi1p and Npr1p by phosphorylation and ubiquitination. Eukaryot Cell. 2008;7:1441-9 pubmed publisher
    ..These data identify Rho5p as being a target of Msi1p/Npr1p regulation and describe a regulatory circuit involving phosphorylation and ubiquitination. ..
  13. Mösch H, Kohler T, Braus G. Different domains of the essential GTPase Cdc42p required for growth and development of Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:235-48 pubmed
  14. Kim J, Jang S, Song K. Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations. Mol Biol Cell. 2008;19:4328-40 pubmed publisher
    ..These findings demonstrate that there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles. ..
  15. Drogen F, O Rourke S, Stucke V, Jaquenoud M, Neiman A, Peter M. Phosphorylation of the MEKK Ste11p by the PAK-like kinase Ste20p is required for MAP kinase signaling in vivo. Curr Biol. 2000;10:630-9 pubmed
    ..This mechanism may serve as a paradigm for the activation of mammalian MEKKs. ..
  16. Zarzov P, Mazzoni C, Mann C. The SLT2(MPK1) MAP kinase is activated during periods of polarized cell growth in yeast. EMBO J. 1996;15:83-91 pubmed
    ..Upstream of BCK1(SLK1), we found that the STE20 kinase was required for SLT2 activation by mating pheromone, but was unnecessary for its activation during the ..
  17. Pryciak P, Hartwell L. AKR1 encodes a candidate effector of the G beta gamma complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction. Mol Cell Biol. 1996;16:2614-26 pubmed
  18. Wang X, Sheff M, Simpson D, Elion E. Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene. BMC Mol Biol. 2011;12:51 pubmed publisher
    ..The patterns of control by Ste11p targets revealed novel functional linkages, cross-regulation, redundancy and compensation. ..
  19. Akada R, Kallal L, Johnson D, Kurjan J. Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics. 1996;143:103-17 pubmed
    ..The other mutations were in STE5 and the STE20 kinase gene, which act near Ste4p in the pathway, and a new gene called STE21...
  20. Bhaduri S, Valk E, Winters M, Gruessner B, Loog M, Pryciak P. A docking interface in the cyclin Cln2 promotes multi-site phosphorylation of substrates and timely cell-cycle entry. Curr Biol. 2015;25:316-25 pubmed publisher
    ..Furthermore, this docking function helps ensure full phosphorylation of substrates with multiple phosphorylation sites, and this contributes to punctual cell-cycle entry. ..
  21. Martin H, Mendoza A, Rodríguez Pachón J, Molina M, Nombela C. Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK-like protein kinase. Mol Microbiol. 1997;23:431-44 pubmed
    b>Ste20/PAK serine/threonine protein kinases have been suggested as playing essential roles in cell signalling and morphogenesis as potential targets of Cdc42 and Rac GTPases...
  22. Jacoby J, Nilius S, Heinisch J. A screen for upstream components of the yeast protein kinase C signal transduction pathway identifies the product of the SLG1 gene. Mol Gen Genet. 1998;258:148-55 pubmed
    ..suppressed by overexpression of genes for other components of the Pkc pathway, such as PKC1, SLT2, ROM2, and STE20. In addition, a SLG1-lacZ reporter construct shows higher expression in the presence of caffeine or magnesium ..
  23. Gorelik M, Davidson A. Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface. J Biol Chem. 2012;287:9168-77 pubmed publisher
    ..To investigate this unusual behavior, we have solved the structure of the Nbp2p SH3-Ste20 peptide complex and compared it with the previously determined structure of the Bem1p SH3b bound to the same ..
  24. Reiser V, Salah S, Ammerer G. Polarized localization of yeast Pbs2 depends on osmostress, the membrane protein Sho1 and Cdc42. Nat Cell Biol. 2000;2:620-7 pubmed
    ..Sho1 itself accumulates at sites of polar growth, but independently of stress conditions and Cdc42. These observations allow us to define the sequence of events that occurs during propogation of osmostress signals. ..
  25. Tiedje C, Holland D, Just U, Höfken T. Proteins involved in sterol synthesis interact with Ste20 and regulate cell polarity. J Cell Sci. 2007;120:3613-24 pubmed
    The Saccharomyces cerevisiae p21-activated kinase (PAK) Ste20 regulates various aspects of cell polarity during vegetative growth, mating and filamentous growth...
  26. Zeller C, Parnell S, Dohlman H. The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem. 2007;282:25168-76 pubmed
    ..Our findings reveal the existence of an unusual Gbeta subunit, one having multiple functions within the cell in addition to serving as a signal transducer for cell surface receptors and intracellular effectors. ..
  27. Gandhi M, Goode B, Chan C. Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Genetics. 2006;174:665-78 pubmed
    ..Our analysis of the second pair of gic1 gic2 suppressors, VHS2 and MLF3, suggests that they regulate polarization of the actin cytoskeleton and cell growth and function in a pathway distinct from and parallel to GIC1 and GIC2. ..
  28. Cullen P, Sabbagh W, Graham E, Irick M, van Olden E, Neal C, et al. A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev. 2004;18:1695-708 pubmed
    ..Taken together, our data suggest that Msb2 is a signaling mucin that interacts with general components, such as Cdc42 and Sho1, to promote their function in the FG pathway. ..
  29. Winters M, Pryciak P. Interaction with the SH3 domain protein Bem1 regulates signaling by the Saccharomyces cerevisiae p21-activated kinase Ste20. Mol Cell Biol. 2005;25:2177-90 pubmed
    The Saccharomyces cerevisiae PAK (p21-activated kinase) family kinase Ste20 functions in several signal transduction pathways, including pheromone response, filamentous growth, and hyperosmotic resistance...
  30. Leberer E, Dignard D, Harcus D, Hougan L, Whiteway M, Thomas D. Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1. Mol Gen Genet. 1993;241:241-54 pubmed
    ..A protein kinase homologue encoded by the STE20 gene has recently been identified as a potential G beta gamma target...
  31. Yamaguchi Y, Ota K, Ito T. A novel Cdc42-interacting domain of the yeast polarity establishment protein Bem1. Implications for modulation of mating pheromone signaling. J Biol Chem. 2007;282:29-38 pubmed
    ..interacts not only with Cdc42 but also with Cdc24 and the effectors of Cdc42, including the p21-activated kinase Ste20, to function as a scaffold for cell polarity establishment...
  32. O Rourke S, Herskowitz I. A third osmosensing branch in Saccharomyces cerevisiae requires the Msb2 protein and functions in parallel with the Sho1 branch. Mol Cell Biol. 2002;22:4739-49 pubmed
    ..These observations indicate that Msb2 is partially redundant with the Sho1 osmosensing branch for the activation of Ste11. ..
  33. Lin M, Li S, Kane P, Höfken T. Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae. Eukaryot Cell. 2012;11:442-51 pubmed publisher
    In the budding yeast Saccharomyces cerevisiae, the Cdc42 effector Ste20 plays a crucial role in the regulation of filamentous growth, a response to nutrient limitation...
  34. Oda Y, Huang K, Cross F, Cowburn D, Chait B. Accurate quantitation of protein expression and site-specific phosphorylation. Proc Natl Acad Sci U S A. 1999;96:6591-6 pubmed
    ..versus mutant cell populations and to the identification of in vivo phosphorylation sites in the PAK-related yeast Ste20 protein kinase that depend specifically on the G1 cyclin Cln2...
  35. Pultz D, Bennetzen M, Rødkær S, Zimmermann C, Enserink J, Andersen J, et al. Global mapping of protein phosphorylation events identifies Ste20, Sch9 and the cell-cycle regulatory kinases Cdc28/Pho85 as mediators of fatty acid starvation responses in Saccharomyces cerevisiae. Mol Biosyst. 2012;8:796-803 pubmed publisher
    ..of these phosphorylation events, we have identified the cell cycle kinases Cdc28 and Pho85, the PAK kinase Ste20 as well as the protein kinase Sch9 as central mediators of the cellular response to inhibition of fatty acid ..
  36. Couve A, Hirsch J. Loss of sustained Fus3p kinase activity and the G1 arrest response in cells expressing an inappropriate pheromone receptor. Mol Cell Biol. 1996;16:4478-85 pubmed
  37. Fujita A, Tonouchi A, Hiroko T, Inose F, Nagashima T, Satoh R, et al. Hsl7p, a negative regulator of Ste20p protein kinase in the Saccharomyces cerevisiae filamentous growth-signaling pathway. Proc Natl Acad Sci U S A. 1999;96:8522-7 pubmed
    ..However, deletions of STE20 in haploid and diploid greatly diminished these hsl7-associated phenotypes...
  38. Richman T, Johnson D. Saccharomyces cerevisiae cdc42p GTPase is involved in preventing the recurrence of bud emergence during the cell cycle. Mol Cell Biol. 2000;20:8548-59 pubmed
  39. Geyer C, Colman Lerner A, Brent R. "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections. Proc Natl Acad Sci U S A. 1999;96:8567-72 pubmed
    ..Forward genetic analysis with peptide aptamer "mutagens" should be particularly useful in elucidating genetic networks in organisms and processes for which classical genetics is not feasible. ..
  40. Lamson R, Winters M, Pryciak P. Cdc42 regulation of kinase activity and signaling by the yeast p21-activated kinase Ste20. Mol Cell Biol. 2002;22:2939-51 pubmed
    The Saccharomyces cerevisiae kinase Ste20 is a member of the p21-activated kinase (PAK) family with several functions, including pheromone-responsive signal transduction...
  41. Lin M, Grillitsch K, Daum G, Just U, Höfken T. Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae. FEBS J. 2009;276:7253-64 pubmed
    ..Here, we demonstrate that the deletion of either STE20 or CLA4 results in increased levels of sterols...
  42. Pereira G, Schiebel E. Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol Cell. 2005;19:209-21 pubmed
    ..In response to spindle misalignment, Kin4 and Bub2-Bfa1 are brought together at both SPBs. Kin4 now maintains Bub2-Bfa1 activity by counteracting Cdc5, thereby inhibiting mitotic exit. ..
  43. Lusk C, Waller D, Makhnevych T, Dienemann A, Whiteway M, Thomas D, et al. Nup53p is a target of two mitotic kinases, Cdk1p and Hrr25p. Traffic. 2007;8:647-60 pubmed
    ..The ability of nup53 alleles containing Cdk1p site mutations to complement synthetic defects of nup53 Delta nup170 Delta strains is linked to a function for Nup53p in the spindle assembly checkpoint. ..
  44. Bhaduri S, Pryciak P. Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes. Curr Biol. 2011;21:1615-23 pubmed publisher
    ..Two such proteins, Ste5 and Ste20, are phosphorylated only when Cdk is associated with the G1/S cyclins Cln1 and Cln2 and not G1, S, or M cyclins...
  45. Takaku T, Ogura K, Kumeta H, Yoshida N, Inagaki F. Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42. J Biol Chem. 2010;285:19346-53 pubmed publisher
    ..Next, the solution structure of Bem1 SH3b-CI in complex with the proline-rich region of p21-activated kinase Ste20 (Ste20 PRR) was determined...
  46. Atkins B, Yoshida S, Saito K, Wu C, Lew D, Pellman D. Inhibition of Cdc42 during mitotic exit is required for cytokinesis. J Cell Biol. 2013;202:231-40 pubmed publisher
    ..The effects of Cdc42 hyperactivation are largely mediated by the Cdc42 effector p21-activated kinase Ste20. Inhibition of Cdc42 and related Rho guanosine triphosphatases may be a general feature of cytokinesis in ..
  47. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..Thus, the reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions. ..
  48. Richman T, Sawyer M, Johnson D. The Cdc42p GTPase is involved in a G2/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast. J Biol Chem. 1999;274:16861-70 pubmed
  49. Gladfelter A, Moskow J, Zyla T, Lew D. Isolation and characterization of effector-loop mutants of CDC42 in yeast. Mol Biol Cell. 2001;12:1239-55 pubmed
    ..The availability of partial function alleles of CDC42 in a genetically tractable system serves as a useful starting point for genetic approaches to identify such novel effectors. ..
  50. Leberer E, Wu C, Leeuw T, Fourest Lieuvin A, Segall J, Thomas D. Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO J. 1997;16:83-97 pubmed
    ..These results suggest that Ste20p is regulated in different developmental pathways by different mechanisms which involve heterotrimeric and small GTP binding proteins. ..
  51. Radcliffe P, Binley K, Trevethick J, Hall M, Sudbery P. Filamentous growth of the budding yeast Saccharomyces cerevisiae induced by overexpression of the WHi2 gene. Microbiology. 1997;143 ( Pt 6):1867-76 pubmed
    ..However, Whi2-induced filament formation is reduced, but not blocked, by mutations in STE7, STE12 or STE20 which do block pseudohypha formation...
  52. Yoon J, Choi E, Parker R. Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiae. J Cell Biol. 2010;189:813-27 pubmed publisher
    ..We show that during stress in Saccharomyces cerevisiae, Dcp2 is phosphorylated on serine 137 by the Ste20 kinase...
  53. Howe A, Fairn G, Macdonald K, Bankaitis V, McMaster C. Regulation of phosphoinositide levels by the phospholipid transfer protein Sec14p controls Cdc42p/p21-activated kinase-mediated cell cycle progression at cytokinesis. Eukaryot Cell. 2007;6:1814-23 pubmed
    ..4-kinases and phosphatidylinositol 4-phosphate 5-kinase prevented growth arrest by CDC42, CLA4, or STE20 upon inactivation of Sec14p function...
  54. Schrick K, Garvik B, Hartwell L. Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone. Genetics. 1997;147:19-32 pubmed
    ..Cells mutant for components of the mitogen-activated protein (MAP) kinase cascade (ste5, ste20, ste11, ste7 or fus3 kss1) formed diploids at a frequency 1% that of the wild-type control, but formed prezygotes ..
  55. Leza M, Elion E. POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2. Genetics. 1999;151:531-43 pubmed
    ..upregulation of the CLN2 gene and that the resulting Cln2 protein promotes recovery primarily through an effect on Ste20, an activator of the mating MAPK cascade...
  56. Tanaka K, Tatebayashi K, Nishimura A, Yamamoto K, Yang H, Saito H. Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms. Sci Signal. 2014;7:ra21 pubmed publisher
    ..Bem1 bound to the cytoplasmic domain of Msb2 and thus recruited the kinases Ste20 and Cla4 to the membrane, where either of them can activate the kinase Ste11...
  57. Takahashi S, Pryciak P. Identification of novel membrane-binding domains in multiple yeast Cdc42 effectors. Mol Biol Cell. 2007;18:4945-56 pubmed
    ..regulates polarity and mitogen-activated protein (MAP) kinase signaling in part through the PAK-family kinase Ste20. Activation of Ste20 requires a Cdc42/Rac interactive binding (CRIB) domain, which mediates its recruitment to ..
  58. Keniry M, Sprague G. Identification of p21-activated kinase specificity determinants in budding yeast: a single amino acid substitution imparts Ste20 specificity to Cla4. Mol Cell Biol. 2003;23:1569-80 pubmed
    Two closely related p21-activated kinases from Saccharomyces cerevisiae, Ste20 and Cla4, interact with and are regulated by Cdc42, a small Rho-like GTPase...
  59. Yamamoto K, Tatebayashi K, Tanaka K, Saito H. Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor. Mol Cell. 2010;40:87-98 pubmed publisher
    ..Thus, dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network. ..
  60. Leberer E, Chenevert J, Leeuw T, Harcus D, Herskowitz I, Thomas D. Genetic interactions indicate a role for Mdg1p and the SH3 domain protein Bem1p in linking the G-protein mediated yeast pheromone signalling pathway to regulators of cell polarity. Mol Gen Genet. 1996;252:608-21 pubmed
    ..The STE20 gene, encoding a protein kinase required for pheromone signal transduction, has recently been identified in a ..
  61. Lin M, Unden H, Jacquier N, Schneiter R, Just U, Höfken T. The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell. 2009;20:4826-37 pubmed publisher
    ..Rho-type GTPase Cdc42 regulates polarized growth through its effectors, including the p21-activated kinases (PAKs) Ste20, Cla4, and Skm1...
  62. Richman T, Toenjes K, Morales S, Cole K, Wasserman B, Taylor C, et al. Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth. Curr Genet. 2004;45:339-49 pubmed
    ..Taken together, these results provide insight into the complicated nature of the relationships between Cdc42p localization, nucleotide binding, and protein-protein interactions. ..
  63. Wu H, Turner C, Gardner J, Temple B, Brennwald P. The Exo70 subunit of the exocyst is an effector for both Cdc42 and Rho3 function in polarized exocytosis. Mol Biol Cell. 2010;21:430-42 pubmed publisher
    ..These data suggest that interaction with the Exo70 component of the exocyst is a key event in spatial regulation of exocytosis by Rho GTPases. ..
  64. Leeuw T, Wu C, Schrag J, Whiteway M, Thomas D, Leberer E. Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases. Nature. 1998;391:191-5 pubmed
    Serine/threonine protein kinases of the Ste20/PAK family have been implicated in the signalling from heterotrimeric G proteins to mitogen-activated protein (MAP) kinase cascades...
  65. Cullen P, Schultz J, Horecka J, Stevenson B, Jigami Y, Sprague G. Defects in protein glycosylation cause SHO1-dependent activation of a STE12 signaling pathway in yeast. Genetics. 2000;155:1005-18 pubmed
    ..We specifically suggest that a Sho1 --> Ste20/Ste50 --> Ste11 --> Ste7 --> Kss1 --> Ste12 pathway is responsible for activation of FUS1 ..
  66. Tatebayashi K, Yamamoto K, Tanaka K, Tomida T, Maruoka T, Kasukawa E, et al. Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway. EMBO J. 2006;25:3033-44 pubmed
    ..We found that Cdc42, in addition to binding and activating the PAK-like kinases Ste20 and Cla4, binds to the Ste11-Ste50 complex to bring activated Ste20/Cla4 to their substrate Ste11...
  67. Mösch H, Kübler E, Krappmann S, Fink G, Braus G. Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1325-35 pubmed
  68. Wu C, Leeuw T, Leberer E, Thomas D, Whiteway M. Cell cycle- and Cln2p-Cdc28p-dependent phosphorylation of the yeast Ste20p protein kinase. J Biol Chem. 1998;273:28107-15 pubmed
    Ste20p from Saccharomyces cerevisiae is a member of the Ste20/p21-activated protein kinase family of protein kinases...
  69. Bhattacharjya S, Gingras R, Xu P. An NMR-based identification of a peptide fragment from the beta-subunit of a G-protein showing specific interactions with the GBB domain of the Ste20 kinase in budding yeast. Biochem Biophys Res Commun. 2006;347:1145-50 pubmed
    ..by interactions between the beta-subunit of a G-protein (G-beta) and the G-beta binding (GBB) domain of Ste20 kinase...
  70. Niu W, Li Z, Zhan W, Iyer V, Marcotte E. Mechanisms of cell cycle control revealed by a systematic and quantitative overexpression screen in S. cerevisiae. PLoS Genet. 2008;4:e1000120 pubmed publisher
    ..This work thus implicates new genes in cell cycle progression, complements previous screens, and lays the foundation for future experiments to define more precisely roles for these genes in cell cycle progression. ..
  71. Simon M, De Virgilio C, Souza B, Pringle J, Abo A, Reed S. Role for the Rho-family GTPase Cdc42 in yeast mating-pheromone signal pathway. Nature. 1995;376:702-5 pubmed
    ..But here we show that Cdc42 has a direct signalling role in the mating-pheromone response between the G protein and the downstream protein kinase cascade. ..
  72. Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher
    ..Taken together, our findings show that budding yeast can access a stable constitutive pseudohyphal growth state with very few genetic and regulatory changes. ..