Gene Symbol: STE18
Description: Ste18p
Alias: Ste18p
Species: Saccharomyces cerevisiae S288c
Products:     STE18

Top Publications

  1. Pryciak P, Hartwell L. AKR1 encodes a candidate effector of the G beta gamma complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction. Mol Cell Biol. 1996;16:2614-26 pubmed
  2. Schrick K, Garvik B, Hartwell L. Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone. Genetics. 1997;147:19-32 pubmed
    ..In contrast, cells mutant for the receptor (ste2) or the beta or gamma subunit (ste4 and ste18) of the G protein were extremely defective in both diploid and prezygote formation and discriminated poorly ..
  3. Cismowski M, Metodiev M, Draper E, Stone D. Biochemical analysis of yeast G(alpha) mutants that enhance adaptation to pheromone. Biochem Biophys Res Commun. 2001;284:247-54 pubmed
    ..A combination of G(alpha) affinity chromatography, GTP binding/hydrolysis studies, and genetic analysis allowed us to assign a distinct mechanism of action to each of these mutant proteins. ..
  4. Hasson M, Blinder D, Thorner J, Jenness D. Mutational activation of the STE5 gene product bypasses the requirement for G protein beta and gamma subunits in the yeast pheromone response pathway. Mol Cell Biol. 1994;14:1054-65 pubmed pheromone-inducible transcription and the sterility phenotype caused by null alleles of the STE2, STE4, or STE18 gene, indicating that the STE5 product acts after the receptor (STE2 product) and after the G protein beta and ..
  5. Clark K, Dignard D, Thomas D, Whiteway M. Interactions among the subunits of the G protein involved in Saccharomyces cerevisiae mating. Mol Cell Biol. 1993;13:1-8 pubmed
    The SCG1 (GPA1), STE4, and STE18 genes of Saccharomyces cerevisiae encode mating-pathway components whose amino acid sequences are similar to those of the alpha, beta, and gamma subunits, respectively, of mammalian G proteins...
  6. Dowell S, Bishop A, Dyos S, Brown A, Whiteway M. Mapping of a yeast G protein betagamma signaling interaction. Genetics. 1998;150:1407-17 pubmed
    ..Ste4p mutants defective in the Ste5p interaction interact efficiently with Gpa1p (Galpha) and Ste18p (Ggamma) but cannot function in signal transduction because cells expressing these mutants are sterile...
  7. Yu R, Pesce C, Colman Lerner A, Lok L, Pincus D, Serra E, et al. Negative feedback that improves information transmission in yeast signalling. Nature. 2008;456:755-61 pubmed publisher
    ..Our work suggests that negative feedback is a general mechanism used in signalling systems to align dose responses and thereby increase the fidelity of information transmission. ..
  8. Oishi K, Kurahashi H, Pack C, Sako Y, Nakamura Y. A bipolar functionality of Q/N-rich proteins: Lsm4 amyloid causes clearance of yeast prions. Microbiologyopen. 2013;2:415-30 pubmed publisher
    ..We also found that the antiprion activity is a general property of [PSI(+) ]-inducible factors. These data provoked a novel "unified" model that explains both prion induction and elimination by a single scheme. ..
  9. Hemsley P, Grierson C. The ankyrin repeats and DHHC S-acyl transferase domain of AKR1 act independently to regulate switching from vegetative to mating states in yeast. PLoS ONE. 2011;6:e28799 pubmed publisher
    ..Proteins similar to AKR1 are found in all eukaryotes and our results have broad implications for future work on these proteins and the control of switching between G?? regulated pathways. ..

More Information


  1. Butty A, Pryciak P, Huang L, Herskowitz I, Peter M. The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating. Science. 1998;282:1511-6 pubmed
    ..Thus, Far1p functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling marked by Gbetagamma to polarize assembly of the cytoskeleton in a morphogenetic gradient. ..
  2. Song J, Hirschman J, Gunn K, Dohlman H. Regulation of membrane and subunit interactions by N-myristoylation of a G protein alpha subunit in yeast. J Biol Chem. 1996;271:20273-83 pubmed
    ..These data suggest that myristoylation is required for specific targeting of Gpa1p to the plasma membrane, where it is needed to interact with the receptor and to regulate the release of Gbetagamma. ..
  3. Dues G, Muller S, Johnsson N. Detection of a conformational change in G gamma upon binding G beta in living cells. FEBS Lett. 2001;505:75-80 pubmed
    ..Ste4p (G beta) and Ste18p (G gamma) are the subunits of a heterotrimeric G-protein in the yeast Saccharomyces cerevisiae...
  4. Li J, Lu N, Huang B. [Roles of G-protein beta and gamma subunits in the interaction of G beta gamma with adenylyl cyclases II]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001;23:115-8 pubmed
  5. Whiteway M, Hougan L, Dignard D, Bell L, Saari G, Grant F, et al. Function of the STE4 and STE18 genes in mating pheromone signal transduction in Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol. 1988;53 Pt 2:585-90 pubmed
  6. Strickfaden S, Pryciak P. Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein. Mol Biol Cell. 2008;19:181-97 pubmed
    ..These findings raise the possibility that the Galphabetagamma heterotrimer can function in a partially dissociated state, tethered by the N-terminal interface. ..
  7. Pryciak P, Huntress F. Membrane recruitment of the kinase cascade scaffold protein Ste5 by the Gbetagamma complex underlies activation of the yeast pheromone response pathway. Genes Dev. 1998;12:2684-97 pubmed
    ..Moreover, our results suggest that this event promotes kinase cascade activation by delivering the Ste5-associated kinases to the cell surface kinase Ste20, whose function may depend on Cdc42 and Cdc24. ..
  8. Ismael A, Tian W, Waszczak N, Wang X, Cao Y, Suchkov D, et al. G? promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation. Sci Signal. 2016;9:ra38 pubmed publisher
  9. Bar E, Ellicott A, Stone D. Gbetagamma recruits Rho1 to the site of polarized growth during mating in budding yeast. J Biol Chem. 2003;278:21798-804 pubmed
    ..Together, these results suggest that Gbetagamma recruits Rho1 to the site of polarized growth during mating. ..
  10. Whiteway M, Dignard D, Thomas D. Mutagenesis of Ste18, a putative G gamma subunit in the Saccharomyces cerevisiae pheromone response pathway. Biochem Cell Biol. 1992;70:1230-7 pubmed
    The yeast STE18 gene product has sequence and functional similarity to the gamma subunits of G proteins. The cloned STE18 gene was subjected to a saturation mutagenesis using doped oligonucleotides...
  11. Hirschman J, De Zutter G, Simonds W, Jenness D. The G beta gamma complex of the yeast pheromone response pathway. Subcellular fractionation and protein-protein interactions. J Biol Chem. 1997;272:240-8 pubmed
    ..The Ste4p and Ste18p proteins that had been extracted from plasma membranes with detergent were found to co-sediment as an 8 S particle ..
  12. Nomoto S, Nakayama N, Arai K, Matsumoto K. Regulation of the yeast pheromone response pathway by G protein subunits. EMBO J. 1990;9:691-6 pubmed
    The yeast GPA1, STE4, and STE18 genes encode proteins homologous to the respective alpha, beta and gamma subunits of the mammalian G protein complex which appears to mediate the response to mating pheromones...
  13. Cole G, Stone D, Reed S. Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway. Mol Cell Biol. 1990;10:510-7 pubmed
    The Saccharomyces cerevisiae GPA1, STE4, and STE18 genes encode products homologous to mammalian G-protein alpha, beta, and gamma subunits, respectively...
  14. Akada R, Kallal L, Johnson D, Kurjan J. Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics. 1996;143:103-17 pubmed
    The Saccharomyces cerevisiae G protein beta gamma dimer, Ste4p/Ste18p, acts downstream of the alpha subunit, Gpa1p, to activate the pheromone response pathway and therefore must interact with a downstream effector...
  15. Yu Y, Hirsch J. An essential gene pair in Saccharomyces cerevisiae with a potential role in mating. DNA Cell Biol. 1995;14:411-8 pubmed
    ..Overexpression of STE5, STE18 (which encodes the G gamma subunit), and a previously unidentified gene, termed SSF1, partially suppressed the ..
  16. Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher
    ..Unlike classical pseudo-hyphal growth, sadF signaling requires Ste5, Ste4 and Ste18, the scaffold protein and G-protein β and γ subunits from the pheromone response pathway, respectively...