Genomes and Genes
Gene Symbol: STE18
Species: Saccharomyces cerevisiae S288c
- Butty A, Pryciak P, Huang L, Herskowitz I, Peter M. The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating. Science. 1998;282:1511-6 pubmed..Thus, Far1p functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling marked by Gbetagamma to polarize assembly of the cytoskeleton in a morphogenetic gradient. ..
- Song J, Hirschman J, Gunn K, Dohlman H. Regulation of membrane and subunit interactions by N-myristoylation of a G protein alpha subunit in yeast. J Biol Chem. 1996;271:20273-83 pubmed..These data suggest that myristoylation is required for specific targeting of Gpa1p to the plasma membrane, where it is needed to interact with the receptor and to regulate the release of Gbetagamma. ..
- Dues G, Muller S, Johnsson N. Detection of a conformational change in G gamma upon binding G beta in living cells. FEBS Lett. 2001;505:75-80 pubmed..Ste4p (G beta) and Ste18p (G gamma) are the subunits of a heterotrimeric G-protein in the yeast Saccharomyces cerevisiae...
- Li J, Lu N, Huang B. [Roles of G-protein beta and gamma subunits in the interaction of G beta gamma with adenylyl cyclases II]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001;23:115-8 pubmed
- Whiteway M, Hougan L, Dignard D, Bell L, Saari G, Grant F, et al. Function of the STE4 and STE18 genes in mating pheromone signal transduction in Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol. 1988;53 Pt 2:585-90 pubmed
- Strickfaden S, Pryciak P. Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein. Mol Biol Cell. 2008;19:181-97 pubmed..These findings raise the possibility that the Galphabetagamma heterotrimer can function in a partially dissociated state, tethered by the N-terminal interface. ..
- Pryciak P, Huntress F. Membrane recruitment of the kinase cascade scaffold protein Ste5 by the Gbetagamma complex underlies activation of the yeast pheromone response pathway. Genes Dev. 1998;12:2684-97 pubmed..Moreover, our results suggest that this event promotes kinase cascade activation by delivering the Ste5-associated kinases to the cell surface kinase Ste20, whose function may depend on Cdc42 and Cdc24. ..
- Bar E, Ellicott A, Stone D. Gbetagamma recruits Rho1 to the site of polarized growth during mating in budding yeast. J Biol Chem. 2003;278:21798-804 pubmed..Together, these results suggest that Gbetagamma recruits Rho1 to the site of polarized growth during mating. ..
- Whiteway M, Dignard D, Thomas D. Mutagenesis of Ste18, a putative G gamma subunit in the Saccharomyces cerevisiae pheromone response pathway. Biochem Cell Biol. 1992;70:1230-7 pubmedThe yeast STE18 gene product has sequence and functional similarity to the gamma subunits of G proteins. The cloned STE18 gene was subjected to a saturation mutagenesis using doped oligonucleotides...
- Hirschman J, De Zutter G, Simonds W, Jenness D. The G beta gamma complex of the yeast pheromone response pathway. Subcellular fractionation and protein-protein interactions. J Biol Chem. 1997;272:240-8 pubmed..The Ste4p and Ste18p proteins that had been extracted from plasma membranes with detergent were found to co-sediment as an 8 S particle ..
- Nomoto S, Nakayama N, Arai K, Matsumoto K. Regulation of the yeast pheromone response pathway by G protein subunits. EMBO J. 1990;9:691-6 pubmedThe yeast GPA1, STE4, and STE18 genes encode proteins homologous to the respective alpha, beta and gamma subunits of the mammalian G protein complex which appears to mediate the response to mating pheromones...
- Cole G, Stone D, Reed S. Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway. Mol Cell Biol. 1990;10:510-7 pubmedThe Saccharomyces cerevisiae GPA1, STE4, and STE18 genes encode products homologous to mammalian G-protein alpha, beta, and gamma subunits, respectively...
- Akada R, Kallal L, Johnson D, Kurjan J. Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics. 1996;143:103-17 pubmedThe Saccharomyces cerevisiae G protein beta gamma dimer, Ste4p/Ste18p, acts downstream of the alpha subunit, Gpa1p, to activate the pheromone response pathway and therefore must interact with a downstream effector...
- Yu Y, Hirsch J. An essential gene pair in Saccharomyces cerevisiae with a potential role in mating. DNA Cell Biol. 1995;14:411-8 pubmed..Overexpression of STE5, STE18 (which encodes the G gamma subunit), and a previously unidentified gene, termed SSF1, partially suppressed the ..
- Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher..Unlike classical pseudo-hyphal growth, sadF signaling requires Ste5, Ste4 and Ste18, the scaffold protein and G-protein Î² and Î³ subunits from the pheromone response pathway, respectively...