STE12

Summary

Gene Symbol: STE12
Description: homeodomain family transcription factor STE12
Alias: homeodomain family transcription factor STE12
Species: Saccharomyces cerevisiae S288c
Products:     STE12

Top Publications

  1. Tedford K, Kim S, Sa D, Stevens K, Tyers M. Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol. 1997;7:228-38 pubmed
    ..proteins, Rst 1 and Rst 2 (also known as Dig1 and Dig2), were found to associate physically with Fus3 and Ste12. Rst1 and Rst2 were prominent substrates in kinase reactions of Fus3 immune complexes from pheromone-treated cells...
  2. Schrick K, Garvik B, Hartwell L. Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone. Genetics. 1997;147:19-32 pubmed
  3. Errede B, Ammerer G. STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. Genes Dev. 1989;3:1349-61 pubmed
    The STE12 gene of Saccharomyces cerevisiae is essential for the expression of genes required for mating, such as those involved in pheromone response, and for genes unrelated to mating but regulated by the presence of an adjacent copy of ..
  4. Madhani H, Fink G. Combinatorial control required for the specificity of yeast MAPK signaling. Science. 1997;275:1314-7 pubmed
    ..Paradoxically, a single downstream transcription factor, Ste12, is necessary for the execution of these distinct programs...
  5. Elion E, Satterberg B, Kranz J. FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell. 1993;4:495-510 pubmed
    ..One substrate is likely to be the transcription factor STE12. A second is likely to be FAR1, a protein required for G1 arrest...
  6. Zheng W, Zhao H, Mancera E, Steinmetz L, Snyder M. Genetic analysis of variation in transcription factor binding in yeast. Nature. 2010;464:1187-91 pubmed publisher
    ..Whole-genome Ste12-binding profiles were determined using chromatin immunoprecipitation coupled with DNA sequencing in pheromone-..
  7. Madhani H, Styles C, Fink G. MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell. 1997;91:673-84 pubmed
    ..Kss1 inhibits the filamentation pathway by interacting with its target transcription factor Ste12. Fus3 has a different inhibitory activity that prevents the inappropriate activation of invasion by the pheromone ..
  8. Printen J, Sprague G. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics. 1994;138:609-19 pubmed
    ..Finally, we detected an interaction between one of the MAP kinases, Kss1p, and a presumptive target, the transcription factor Ste12p. We failed to detect interactions of Ste4p or Ste20p with any other component of the response pathway. ..
  9. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
  10. Herskowitz I. MAP kinase pathways in yeast: for mating and more. Cell. 1995;80:187-97 pubmed

Detail Information

Publications73

  1. Tedford K, Kim S, Sa D, Stevens K, Tyers M. Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol. 1997;7:228-38 pubmed
    ..proteins, Rst 1 and Rst 2 (also known as Dig1 and Dig2), were found to associate physically with Fus3 and Ste12. Rst1 and Rst2 were prominent substrates in kinase reactions of Fus3 immune complexes from pheromone-treated cells...
  2. Schrick K, Garvik B, Hartwell L. Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone. Genetics. 1997;147:19-32 pubmed
  3. Errede B, Ammerer G. STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. Genes Dev. 1989;3:1349-61 pubmed
    The STE12 gene of Saccharomyces cerevisiae is essential for the expression of genes required for mating, such as those involved in pheromone response, and for genes unrelated to mating but regulated by the presence of an adjacent copy of ..
  4. Madhani H, Fink G. Combinatorial control required for the specificity of yeast MAPK signaling. Science. 1997;275:1314-7 pubmed
    ..Paradoxically, a single downstream transcription factor, Ste12, is necessary for the execution of these distinct programs...
  5. Elion E, Satterberg B, Kranz J. FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell. 1993;4:495-510 pubmed
    ..One substrate is likely to be the transcription factor STE12. A second is likely to be FAR1, a protein required for G1 arrest...
  6. Zheng W, Zhao H, Mancera E, Steinmetz L, Snyder M. Genetic analysis of variation in transcription factor binding in yeast. Nature. 2010;464:1187-91 pubmed publisher
    ..Whole-genome Ste12-binding profiles were determined using chromatin immunoprecipitation coupled with DNA sequencing in pheromone-..
  7. Madhani H, Styles C, Fink G. MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell. 1997;91:673-84 pubmed
    ..Kss1 inhibits the filamentation pathway by interacting with its target transcription factor Ste12. Fus3 has a different inhibitory activity that prevents the inappropriate activation of invasion by the pheromone ..
  8. Printen J, Sprague G. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics. 1994;138:609-19 pubmed
    ..Finally, we detected an interaction between one of the MAP kinases, Kss1p, and a presumptive target, the transcription factor Ste12p. We failed to detect interactions of Ste4p or Ste20p with any other component of the response pathway. ..
  9. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
  10. Herskowitz I. MAP kinase pathways in yeast: for mating and more. Cell. 1995;80:187-97 pubmed
  11. Yu R, Pesce C, Colman Lerner A, Lok L, Pincus D, Serra E, et al. Negative feedback that improves information transmission in yeast signalling. Nature. 2008;456:755-61 pubmed publisher
    ..Our work suggests that negative feedback is a general mechanism used in signalling systems to align dose responses and thereby increase the fidelity of information transmission. ..
  12. Chou S, Lane S, Liu H. Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:4794-805 pubmed
    The Saccharomyces cerevisiae transcription factor Ste12 controls two distinct developmental programs of mating and filamentation...
  13. Dolan J, Fields S. Overproduction of the yeast STE12 protein leads to constitutive transcriptional induction. Genes Dev. 1990;4:492-502 pubmed
    ..These responses are dependent on the activity of several genes, including STE12, whose product binds to the pheromone response element located within the regulatory DNA sequences of inducible ..
  14. Cook J, Bardwell L, Kron S, Thorner J. Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev. 1996;10:2831-48 pubmed
    ..alternatives to vegetative proliferation require the MAP kinase Kss1 and the transcriptional transactivator Ste12. Using a two-hybrid screen for gene products that interact with Kss1, two homologous and previously ..
  15. Lee B, Elion E. The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components. Proc Natl Acad Sci U S A. 1999;96:12679-84 pubmed
    ..protein kinase kinase kinase kinase (MAPKKKK, Ste20), MAPKKK (Ste11), MAPKK (Ste7), and transcription factor (Ste12) to promote either G(1) arrest and fusion or foraging in response to distinct stimuli...
  16. Baur M, Esch R, Errede B. Cooperative binding interactions required for function of the Ty1 sterile responsive element. Mol Cell Biol. 1997;17:4330-7 pubmed
    ..These results establish a direct role for Tec1p in the Ty1 SRE and yet another set of combinatorial interactions that achieve a qualitatively distinct mode of transcriptional regulation with Ste12p. ..
  17. Rupp S, Summers E, Lo H, Madhani H, Fink G. MAP kinase and cAMP filamentation signaling pathways converge on the unusually large promoter of the yeast FLO11 gene. EMBO J. 1999;18:1257-69 pubmed
    ..Secondly, mutations in either pathway block FLO11 transcription. Overexpression of STE12 can suppress the loss of FLO8, and overexpression of FLO8 can suppress the loss of STE12...
  18. Bardwell L, Cook J, Voora D, Baggott D, Martinez A, Thorner J. Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK. Genes Dev. 1998;12:2887-98 pubmed
    ..We found that unphosphorylated Kss1 binds directly to the transcription factor Ste12, that this binding is necessary for Kss1-mediated repression of Ste12, and that Ste7-mediated phosphorylation of ..
  19. Roberts R, Fink G. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev. 1994;8:2974-85 pubmed
    ..same components of the MAP kinase cascade necessary for diploid pseudohyphal development (STE20, STE11, STE7, and STE12) are also required for both filament formation and agar penetration in haploids...
  20. Olson K, Nelson C, Tai G, Hung W, Yong C, Astell C, et al. Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms. Mol Cell Biol. 2000;20:4199-209 pubmed
    ..residues 216 to 688) [Ste12p(216-688)] from a GAL promoter causes FUS1 induction in a strain expressing wild-type STE12, suggesting that this region can cause the activation of endogenous Ste12p...
  21. van Dyk D, Pretorius I, Bauer F. Mss11p is a central element of the regulatory network that controls FLO11 expression and invasive growth in Saccharomyces cerevisiae. Genetics. 2005;169:91-106 pubmed
    ..Taken together, the data strongly suggest a central role for Mss11p in the regulatory network controlling FLO11 expression, invasive growth, and pseudohyphal differentiation. ..
  22. Liu H, Styles C, Fink G. Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science. 1993;262:1741-4 pubmed
    ..a signal initiated by peptide pheromones is transmitted through this kinase cascade to a transcription factor STE12, which is required for the expression of many mating-specific genes...
  23. Pi H, Chien C, Fields S. Transcriptional activation upon pheromone stimulation mediated by a small domain of Saccharomyces cerevisiae Ste12p. Mol Cell Biol. 1997;17:6410-8 pubmed
    ..These results suggest that the pheromone induction domain of Ste12p mediates transcriptional induction via a two-step process: the relief of repression and synergistic transcriptional activation with another activation domain. ..
  24. Lo W, Dranginis A. The cell surface flocculin Flo11 is required for pseudohyphae formation and invasion by Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:161-71 pubmed
    ..Yeast with a deletion of STE12 does not express FLO11 transcripts, indicating that STE12 is required for FLO11 expression...
  25. Bickel K, Morris D. Role of the transcription activator Ste12p as a repressor of PRY3 expression. Mol Cell Biol. 2006;26:7901-12 pubmed
    ..PRY3 regulation provides a model for the coordination of both inductive and repressive activities within a regulatory network. ..
  26. Woods K, Höfken T. The zinc cluster proteins Upc2 and Ecm22 promote filamentation in Saccharomyces cerevisiae by sterol biosynthesis-dependent and -independent pathways. Mol Microbiol. 2016;99:512-27 pubmed publisher
    ..The expression of SUT1 and SUT2 in turn is under negative control of the transcription factor Ste12. We suggest that during filamentation Ste12 becomes activated and reduces SUT1/SUT2 expression levels...
  27. Jung D, Ahn J, Rhee B, Kim J. Mutational analysis of the RNA helicase Dhh1 in Ste12 expression and yeast mating. J Microbiol. 2017;55:373-378 pubmed publisher
    ..The dhh1 deletion mutation results in a significant decrease in the expression of Ste12, a mating-specific transcription factor, showing severe mating defects...
  28. Kim T, Kim H, Yoon J, Kang H. Recruitment of the Swi/Snf complex by Ste12-Tec1 promotes Flo8-Mss11-mediated activation of STA1 expression. Mol Cell Biol. 2004;24:9542-56 pubmed
    ..which encodes an extracellular glucoamylase, is activated by the specific DNA-binding activators Flo8, Mss11, Ste12, and Tec1 and the Swi/Snf chromatin-remodeling complex...
  29. Kusari A, Molina D, Sabbagh W, Lau C, Bardwell L. A conserved protein interaction network involving the yeast MAP kinases Fus3 and Kss1. J Cell Biol. 2004;164:267-77 pubmed
    ..The Kss1 mutants were also defective in Kss1-imposed repression of Ste12. We conclude that MAPKs contain a structurally and functionally conserved docking site that mediates an overall ..
  30. Aristizabal M, Negri G, Kobor M. The RNAPII-CTD Maintains Genome Integrity through Inhibition of Retrotransposon Gene Expression and Transposition. PLoS Genet. 2015;11:e1005608 pubmed publisher
    ..contributed to Ty1 gene expression regulation by altering the occupancy of the gene-specific transcription factor Ste12. Loss of STE12 and TEC1 suppressed growth phenotypes of the RNAPII-CTD truncation mutant...
  31. Adhikari H, Vadaie N, Chow J, Caccamise L, Chavel C, Li B, et al. Role of the unfolded protein response in regulating the mucin-dependent filamentous-growth mitogen-activated protein kinase pathway. Mol Cell Biol. 2015;35:1414-32 pubmed publisher
    ..We speculate that the QC pathways broadly regulate signaling glycoproteins and their cognate pathways by recognizing altered glycosylation patterns that can occur in response to extrinsic cues. ..
  32. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..Thus, the reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions. ..
  33. Radcliffe P, Binley K, Trevethick J, Hall M, Sudbery P. Filamentous growth of the budding yeast Saccharomyces cerevisiae induced by overexpression of the WHi2 gene. Microbiology. 1997;143 ( Pt 6):1867-76 pubmed
    ..However, Whi2-induced filament formation is reduced, but not blocked, by mutations in STE7, STE12 or STE20 which do block pseudohypha formation...
  34. Kurihara L, Stewart B, Gammie A, Rose M. Kar4p, a karyogamy-specific component of the yeast pheromone response pathway. Mol Cell Biol. 1996;16:3990-4002 pubmed
    ..A 30-bp region upstream of KAR3 conferred both KAR4- and STE12-dependent induction by mating pheromone...
  35. Breitkreutz A, Boucher L, Tyers M. MAPK specificity in the yeast pheromone response independent of transcriptional activation. Curr Biol. 2001;11:1266-71 pubmed
    ..two related MAPKs, Fus3 and Kss1, which in turn regulate programs of gene expression via the transcription factor Ste12. As fus3, but not kss1, strains are impaired for mating, Fus3 exhibits specificity for the pheromone response...
  36. Pan X, Heitman J. Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:4874-87 pubmed
    ..Activation of filamentous growth by PKA does not require the transcription factors Ste12 and Tec1 of the MAP kinase cascade, Phd1, or the PKA targets Msn2 and Msn4...
  37. Kobayashi J, Matsuura Y. Structural basis for cell-cycle-dependent nuclear import mediated by the karyopherin Kap121p. J Mol Biol. 2013;425:1852-1868 pubmed publisher
    ..Comparison of the NLS and RanGTP complexes reveals that RanGTP binding not only occludes the cargo-binding site but also forces Kap121p into a conformation that is incompatible with NLS recognition...
  38. Chang Y, Wickes B, Miller G, Penoyer L, Kwon Chung K. Cryptococcus neoformans STE12alpha regulates virulence but is not essential for mating. J Exp Med. 2000;191:871-82 pubmed
    The Cryptococcus neoformans STE12alpha gene, a homologue of Saccharomyces cerevisiae STE12, exists only in mating type (MAT)alpha cells. In S. cerevisiae, STE12 was required for mating and filament formation. In C...
  39. Bruhn L, Sprague G. MCM1 point mutants deficient in expression of alpha-specific genes: residues important for interaction with alpha 1. Mol Cell Biol. 1994;14:2534-44 pubmed
    Complexes formed between MCM1 and several coregulatory proteins--alpha 1, alpha 2, and STE12--serve to govern transcription of the a- and alpha-specific gene sets in the yeast Saccharomyces cerevisiae...
  40. Ohkuni K, Kitagawa K. Endogenous transcription at the centromere facilitates centromere activity in budding yeast. Curr Biol. 2011;21:1695-703 pubmed publisher
    ..Furthermore, we have identified Ste12, which is a transcription factor, and Dig1, a Ste12 inhibitor, as a novel CEN-associated protein complex by an in ..
  41. Bester M, Jacobson D, Bauer F. Many Saccharomyces cerevisiae Cell Wall Protein Encoding Genes Are Coregulated by Mss11, but Cellular Adhesion Phenotypes Appear Only Flo Protein Dependent. G3 (Bethesda). 2012;2:131-41 pubmed publisher
    ..This analysis shows that only FLO genes, in particular FLO1/10/11, appear to significantly impact on such phenotypes. Thus adhesion-related phenotypes are primarily dependent on the balance of FLO gene expression. ..
  42. van der Felden J, Weisser S, Bruckner S, Lenz P, Mösch H. The transcription factors Tec1 and Ste12 interact with coregulators Msa1 and Msa2 to activate adhesion and multicellular development. Mol Cell Biol. 2014;34:2283-93 pubmed publisher
    ..and related yeast species, the TEA transcription factor Tec1, together with a second transcription factor, Ste12, controls development, including cell adhesion and filament formation...
  43. Ka M, Park Y, Kim J. The DEAD-box RNA helicase, Dhh1, functions in mating by regulating Ste12 translation in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2008;367:680-6 pubmed publisher
    ..A dhh1 deletion mutation caused a significant decrease in the protein level of Ste12, a mating-specific transcription factor, resulting in severe mating defects...
  44. Patury S, Geda P, Dobry C, Kumar A, Gestwicki J. Conditional nuclear import and export of yeast proteins using a chemical inducer of dimerization. Cell Biochem Biophys. 2009;53:127-34 pubmed publisher
    ..Collectively, these studies provide a necessary framework for the design of large-scale applications. ..
  45. Cullen P, Schultz J, Horecka J, Stevenson B, Jigami Y, Sprague G. Defects in protein glycosylation cause SHO1-dependent activation of a STE12 signaling pathway in yeast. Genetics. 2000;155:1005-18 pubmed
    ..We specifically suggest that a Sho1 --> Ste20/Ste50 --> Ste11 --> Ste7 --> Kss1 --> Ste12 pathway is responsible for activation of FUS1 transcription in these mutants...
  46. Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher
    ..Taken together, our findings show that budding yeast can access a stable constitutive pseudohyphal growth state with very few genetic and regulatory changes. ..
  47. Mösch H, Kübler E, Krappmann S, Fink G, Braus G. Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1325-35 pubmed
  48. Hairfield M, Ayers A, Dolan J. Phospholipase D1 is required for efficient mating projection formation in Saccharomyces cerevisiae. FEMS Yeast Res. 2001;1:225-32 pubmed
    ..The fact that morphogenesis is most dramatically affected indicates that PLD1 functions primarily in the morphogenic branch of the pheromone response pathway. ..
  49. Su T, Tamarkina E, Sadowski I. Organizational constraints on Ste12 cis-elements for a pheromone response in Saccharomyces cerevisiae. FEBS J. 2010;277:3235-48 pubmed publisher
    b>Ste12 of Saccharomyces cerevisiae binds to pheromone-response cis-elements (PREs) to regulate several classes of genes. Genes induced by pheromones require multimerization of Ste12 for binding of at least two PREs on responsive promoters...
  50. Oehlen L, McKinney J, Cross F. Ste12 and Mcm1 regulate cell cycle-dependent transcription of FAR1. Mol Cell Biol. 1996;16:2830-7 pubmed
    ..In the absence of a functional Ste12 transcription factor, both the levels and the cell cycle pattern of expression of these genes were affected...
  51. Nakayama N, Kaziro Y, Arai K, Matsumoto K. Role of STE genes in the mating factor signaling pathway mediated by GPA1 in Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:3777-83 pubmed
    The ste mutants (ste2, ste4, ste5, ste7, ste11, and ste12) are insensitive to mating factors and are, therefore, sterile...
  52. Pryciak P, Hartwell L. AKR1 encodes a candidate effector of the G beta gamma complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction. Mol Cell Biol. 1996;16:2614-26 pubmed
  53. O Rourke S, Herskowitz I. The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev. 1998;12:2874-86 pubmed
    ..Finally, we have found that pseudohyphal growth exhibited by wild-type (HOG1) strains depends on SHO1, suggesting that Sho1p may be a receptor that feeds into the pseudohyphal growth pathway. ..
  54. Wang X, Sheff M, Simpson D, Elion E. Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene. BMC Mol Biol. 2011;12:51 pubmed publisher
    ..Here it is shown that catalytically active Ste11p regulates FKS2-lacZ reporter genes through Ste12, calcineurin/Crz1p- and PKC pathways and the high osmolarity pathway...
  55. Akada R, Kallal L, Johnson D, Kurjan J. Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics. 1996;143:103-17 pubmed
    ..Mutations in pheromone response pathway components did not suppress the lethality associated with the activated CDC42 mutations, suggesting that this effect is independent of the pheromone response pathway. ..
  56. Leslie D, Grill B, Rout M, Wozniak R, Aitchison J. Kap121p-mediated nuclear import is required for mating and cellular differentiation in yeast. Mol Cell Biol. 2002;22:2544-55 pubmed
    ..Furthermore, by overexpression of STE12 or expression of a STE12-cNLS fusion in kap121 cells, the invasive-growth defect and the mating defect were both ..
  57. Ramer S, Davis R. A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993;90:452-6 pubmed
    ..This sterility can be partially suppressed by high-level production of STE12 but is not suppressible by high levels of STE4 or a dominant STE11 truncation allele...
  58. La Roche S, Shafer B, Strathern J. A ste12 allele having a differential effect on a versus alpha cells. Mol Gen Genet. 1995;246:80-90 pubmed
    ..We isolated and characterized a partially functional ste12 allele (ste12-T50), that is defective only in the activation of alpha-specific genes...
  59. Dhillon N, Kamakaka R. A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR. Mol Cell. 2000;6:769-80 pubmed
  60. Wang Y, Dohlman H. Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast. J Biol Chem. 2006;281:1964-9 pubmed
    ..The yeast transcription factor Ste12 is required in at least two distinct signaling processes, each regulated by many of the same protein kinases...
  61. Houser J, Ford E, Nagiec M, Errede B, Elston T. Positive roles for negative regulators in the mating response of yeast. Mol Syst Biol. 2012;8:586 pubmed publisher
    ..Here we combine computational and experimental approaches to study transcriptional regulation mediated by Ste12, the key transcription factor in the pheromone response...
  62. McCullagh E, Seshan A, El Samad H, Madhani H. Coordinate control of gene expression noise and interchromosomal interactions in a MAP kinase pathway. Nat Cell Biol. 2010;12:954-62 pubmed publisher
    In the Saccharomyces cerevisiae pheromone-response pathway, the transcription factor Ste12 is inhibited by two mitogen-activated protein (MAP)-kinase-responsive regulators, Dig1 and Dig2...
  63. Park Y, Hur H, Ka M, Kim J. Identification of translational regulation target genes during filamentous growth in Saccharomyces cerevisiae: regulatory role of Caf20 and Dhh1. Eukaryot Cell. 2006;5:2120-7 pubmed
    ..Signaling pathway-responsive transcription factors such as Ste12, Tec1, and Flo8 are known to mediate filamentation-specific transcription...
  64. Chen R, Thorner J. Systematic epistasis analysis of the contributions of protein kinase A- and mitogen-activated protein kinase-dependent signaling to nutrient limitation-evoked responses in the yeast Saccharomyces cerevisiae. Genetics. 2010;185:855-70 pubmed publisher
    ..Thus, although there are similarities between haploids and diploids, cell type-specific differences clearly alter the balance of the signaling inputs required to elicit the various nutrient limitation-evoked cellular behaviors. ..
  65. Heise B, van der Felden J, Kern S, Malcher M, Bruckner S, Mösch H. The TEA transcription factor Tec1 confers promoter-specific gene regulation by Ste12-dependent and -independent mechanisms. Eukaryot Cell. 2010;9:514-31 pubmed publisher
    ..the TEA transcription factor Tec1 is known to regulate target genes together with a second transcription factor, Ste12. Tec1-Ste12 complexes can activate transcription through Tec1 binding sites (TCSs), which can be further combined ..
  66. Cullen P, Sabbagh W, Graham E, Irick M, van Olden E, Neal C, et al. A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev. 2004;18:1695-708 pubmed
    ..Taken together, our data suggest that Msb2 is a signaling mucin that interacts with general components, such as Cdc42 and Sho1, to promote their function in the FG pathway. ..
  67. Ranish J, Yi E, Leslie D, Purvine S, Goodlett D, Eng J, et al. The study of macromolecular complexes by quantitative proteomics. Nat Genet. 2003;33:349-55 pubmed
    ..In a second application, peptides derived from immunopurified STE12 protein complexes isolated from yeast cells in different states were used to detect quantitative changes in the ..
  68. Nelson C, Goto S, Lund K, Hung W, Sadowski I. Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12. Nature. 2003;421:187-90 pubmed
    ..This response is dependent on the transcription factor Ste12 and on the mating pheromone-response mitogen-activated protein (MAP) kinase cascade, but a mechanism for ..
  69. Park Y, Hwang O, Kim J. Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homolog. Biochem Biophys Res Commun. 2002;293:733-40 pubmed
    ..overexpression promoted filamentation growth of the Sigma1278b wild-type strain and suppressed the filamentation growth defect of the ste12 mutation. These results lead us to a new understanding of cellular functions of the yeast OSBPs.
  70. Mösch H, Fink G. Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics. 1997;145:671-84 pubmed
    ..novel targets of the filamentation signaling pathway, and we thereby identified 16 different genes, CDC39, STE12, TEC1, WHI3, NAB1, DBR1, CDC55, SRV2, TPM1, SPA2, BNI1, DFG5, DFG9, DFG10, BUD8 and DFG16, mutations that block ..
  71. Chandarlapaty S, Errede B. Ash1, a daughter cell-specific protein, is required for pseudohyphal growth of Saccharomyces cerevisiae. Mol Cell Biol. 1998;18:2884-91 pubmed
    ..are consistent with the deduction that Ash1 acts separately from the mitogen-activated protein kinase cascade and Ste12. Similarly to the case in yeast form cells, Ash1 is asymmetrically localized to the nuclei of daughter cells ..
  72. Birkaya B, Maddi A, Joshi J, Free S, Cullen P. Role of the cell wall integrity and filamentous growth mitogen-activated protein kinase pathways in cell wall remodeling during filamentous growth. Eukaryot Cell. 2009;8:1118-33 pubmed publisher
    ..Disruption of ss-1,3-glucan linkages induced mucin shedding and resulted in defects in cell-cell adhesion and invasion of cells into the agar matrix. ..
  73. Raithatha S, Su T, Lourenco P, Goto S, Sadowski I. Cdk8 regulates stability of the transcription factor Phd1 to control pseudohyphal differentiation of Saccharomyces cerevisiae. Mol Cell Biol. 2012;32:664-74 pubmed publisher
    ..PHD1 expression is partially dependent upon Ste12, which was also previously shown to be destabilized by Cdk8-dependent phosphorylations, but to a significantly ..