STE11

Summary

Gene Symbol: STE11
Description: mitogen-activated protein kinase kinase kinase STE11
Alias: mitogen-activated protein kinase kinase kinase STE11
Species: Saccharomyces cerevisiae S288c
Products:     STE11

Top Publications

  1. Grimshaw S, Mott H, Stott K, Nielsen P, Evetts K, Hopkins L, et al. Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM. J Biol Chem. 2004;279:2192-201 pubmed
    ..We have found that L73A and L75A abrogate the Ste50p interaction with Ste11p, and we compare these data with the known interaction sites defined for other SAM domain interactions. ..
  2. Slaughter B, Schwartz J, Li R. Mapping dynamic protein interactions in MAP kinase signaling using live-cell fluorescence fluctuation spectroscopy and imaging. Proc Natl Acad Sci U S A. 2007;104:20320-5 pubmed
    ..interactions of Ste7 (MAPK kinase) with the MAPKs, Fus3 or Kss1, and of the scaffold protein, Ste5, with Ste7 and Ste11 (MAPK kinase kinase) in the cytosol, providing in vivo constants of their binding equilibrium...
  3. Truckses D, Bloomekatz J, Thorner J. The RA domain of Ste50 adaptor protein is required for delivery of Ste11 to the plasma membrane in the filamentous growth signaling pathway of the yeast Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:912-28 pubmed
    ..ensures efficient G-protein-dependent recruitment of mitogen-activated protein kinase (MAPK) cascade components Ste11 (MAPK kinase kinase), Ste7 (MAPK kinase), and Fus3 (MAPK) to the plasma membrane for activation by Ste20 protein ..
  4. Kwan J, Warner N, Maini J, Chan Tung K, Zakaria H, Pawson T, et al. Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction. J Mol Biol. 2006;356:142-54 pubmed
    ..and nitrogen starvation depends upon a direct interaction between the sterile alpha motif (SAM) domains of the Ste11 mitogen-activated protein kinase kinase kinase (MAPKKK) and its regulator Ste50...
  5. O Rourke S, Herskowitz I. Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis. Mol Biol Cell. 2004;15:532-42 pubmed
    ..characterized the roles of common pathway components (Hog1 and Pbs2) and components in the two upstream branches (Ste11, Sho1, and Ssk1) in response to elevated osmolarity by using whole-genome expression profiling...
  6. Marcus S, Polverino A, Barr M, Wigler M. Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc Natl Acad Sci U S A. 1994;91:7762-6 pubmed
    We present genetic evidence for complex formation of STE5 and the STE11, STE7, and FUS3 protein kinases, the pheromone-responsive mitogen-activated protein kinase module of Saccharomyces cerevisiae...
  7. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..Thus, the reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions. ..
  8. Liu H, Styles C, Fink G. Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science. 1993;262:1741-4 pubmed
    ..Thus, a similar kinase cascade is activated by different signals in haploids and diploids and mediates different developmental outcomes in the two cell types. ..
  9. Bhattacharjya S, Xu P, Gingras R, Shaykhutdinov R, Wu C, Whiteway M, et al. Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex. J Mol Biol. 2004;344:1071-87 pubmed
    b>Ste11, a homologue of mammalian MAPKKKs, together with its binding partner Ste50 works in a number of MAPK signaling pathways of Saccharomyces cerevisiae...

More Information

Publications79

  1. Madhani H, Styles C, Fink G. MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell. 1997;91:673-84 pubmed
    ..In the absence of Fus3, there is erroneous crosstalk in which mating pheromone now activates filamentation-specific gene expression using the Kss1 MAPK. ..
  2. Inouye C, Dhillon N, Durfee T, Zambryski P, Thorner J. Mutational analysis of STE5 in the yeast Saccharomyces cerevisiae: application of a differential interaction trap assay for examining protein-protein interactions. Genetics. 1997;147:479-92 pubmed
    ..that differentially affect the ability of Ste5 to interact with either of two MAPK cascade constituents, the MEKK (Ste11) and the MEK (Ste7)...
  3. Slaughter B, Huff J, Wiegraebe W, Schwartz J, Li R. SAM domain-based protein oligomerization observed by live-cell fluorescence fluctuation spectroscopy. PLoS ONE. 2008;3:e1931 pubmed publisher
    ..the oligomerization and binding stoichiometry of high-order, multi-component complexes of (SAM) domain proteins Ste11 and Ste50 in live yeast cells using fluorescence fluctuation methods...
  4. Madhani H, Galitski T, Lander E, Fink G. Effectors of a developmental mitogen-activated protein kinase cascade revealed by expression signatures of signaling mutants. Proc Natl Acad Sci U S A. 1999;96:12530-5 pubmed
    ..Thus, the Kss1 MAPK cascade programs development by coordinately modulating a cell adhesion factor, a secreted host-destroying activity, and a specialized subunit of the Cdc28 cyclin-dependent kinase. ..
  5. Tatebayashi K, Yamamoto K, Tanaka K, Tomida T, Maruoka T, Kasukawa E, et al. Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway. EMBO J. 2006;25:3033-44 pubmed
    ..When stimulated by high osmolarity, the SHO1 branch activates an MAP kinase module composed of the Ste11 MAPKKK, the Pbs2 MAPKK, and the Hog1 MAPK...
  6. Wang X, Sheff M, Simpson D, Elion E. Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene. BMC Mol Biol. 2011;12:51 pubmed publisher
    ..The patterns of control by Ste11p targets revealed novel functional linkages, cross-regulation, redundancy and compensation. ..
  7. Bhattacharjya S, Xu P, Chakrapani M, Johnston L, Ni F. Polymerization of the SAM domain of MAPKKK Ste11 from the budding yeast: implications for efficient signaling through the MAPK cascades. Protein Sci. 2005;14:828-35 pubmed
    ..The SAM domain of the yeast MAPKKK Ste11 has a well-folded dimeric structure in solution...
  8. Posas F, Saito H. Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK. Science. 1997;276:1702-5 pubmed
    ..The MAPKK Pbs2p bound to the Sho1p osmosensor, the MAPKKK Ste11p, and the MAPK Hog1p. Thus, Pbs2p may serve as a scaffold protein. ..
  9. Tanigawa M, Kihara A, Terashima M, Takahara T, Maeda T. Sphingolipids regulate the yeast high-osmolarity glycerol response pathway. Mol Cell Biol. 2012;32:2861-70 pubmed publisher
    ..These observations reveal the sphingolipid-mediated regulation of the osmosensing machinery of the HOG pathway...
  10. Wu C, Jansen G, Zhang J, Thomas D, Whiteway M. Adaptor protein Ste50p links the Ste11p MEKK to the HOG pathway through plasma membrane association. Genes Dev. 2006;20:734-46 pubmed
    ..We propose that Opy2p can serve as a membrane anchor for the Ste50p/Ste11p module in the activation of the HOG pathway. ..
  11. Ramezani Rad M, Jansen G, Buhring F, Hollenberg C. Ste50p is involved in regulating filamentous growth in the yeast Saccharomyces cerevisiae and associates with Ste11p. Mol Gen Genet. 1998;259:29-38 pubmed
    ..Overexpression of STE11 also suppresses the mating defects of ste50 mutants...
  12. Posas F, Witten E, Saito H. Requirement of STE50 for osmostress-induced activation of the STE11 mitogen-activated protein kinase kinase kinase in the high-osmolarity glycerol response pathway. Mol Cell Biol. 1998;18:5788-96 pubmed
    ..protein (MAP) kinase cascade, which is composed of three tiers of protein kinases: (i) the SSK2, SSK22, and STE11 MAP kinase kinase kinases (MAPKKKs), (ii) the PBS2 MAPKK, and (iii) the HOG1 MAP kinase...
  13. Choi K, Satterberg B, Lyons D, Elion E. Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell. 1994;78:499-512 pubmed
    Ste5 is a Zn2+ finger-like protein thought to function before three kinases, Ste11 (a MEKK), Ste7 (a MEK), and Fus3 (a MAPK), in a conserved MAP kinase cascade required for mating in S. cerevisiae...
  14. Bhunia A, Domadia P, Mohanram H, Bhattacharjya S. NMR structural studies of the Ste11 SAM domain in the dodecyl phosphocholine micelle. Proteins. 2009;74:328-43 pubmed publisher
    ..The Ste11 protein kinase from the mitogen activated protein kinase (MAPK) signaling cascades of the budding yeast is ..
  15. Roberts R, Fink G. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev. 1994;8:2974-85 pubmed
    ..We find that the same components of the MAP kinase cascade necessary for diploid pseudohyphal development (STE20, STE11, STE7, and STE12) are also required for both filament formation and agar penetration in haploids...
  16. Printen J, Sprague G. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics. 1994;138:609-19 pubmed
    ..Finally, we detected an interaction between one of the MAP kinases, Kss1p, and a presumptive target, the transcription factor Ste12p. We failed to detect interactions of Ste4p or Ste20p with any other component of the response pathway. ..
  17. Zarrinpar A, Bhattacharyya R, Nittler M, Lim W. Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway. Mol Cell. 2004;14:825-32 pubmed
    ..is thought to function as a scaffold, since it binds the osmosensor Sho1, the upstream MAP kinase kinase kinase Ste11, and the downstream MAP kinase Hog1...
  18. O Rourke S, Herskowitz I. The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev. 1998;12:2874-86 pubmed
    ..Finally, we have found that pseudohyphal growth exhibited by wild-type (HOG1) strains depends on SHO1, suggesting that Sho1p may be a receptor that feeds into the pseudohyphal growth pathway. ..
  19. Kwan J, Warner N, Pawson T, Donaldson L. The solution structure of the S.cerevisiae Ste11 MAPKKK SAM domain and its partnership with Ste50. J Mol Biol. 2004;342:681-93 pubmed
    b>Ste11 is a MAPKKK from Saccharomyces cerevisiae that helps mediate the response to mating pheromone and the ability to thrive in high-salt environments...
  20. Maeder C, Hink M, Kinkhabwala A, Mayr R, Bastiaens P, Knop M. Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol. 2007;9:1319-26 pubmed
    ..We quantified the abundance of complexes in the cytoplasm among the MAPKs Ste11, Ste7, Fus3 and the scaffold protein Ste5 in yeast pheromone signalling using fluorescence cross-correlation ..
  21. Lee B, Elion E. The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components. Proc Natl Acad Sci U S A. 1999;96:12679-84 pubmed
    ..growth (IG) pathways use the same mitogen-activated protein kinase kinase kinase kinase (MAPKKKK, Ste20), MAPKKK (Ste11), MAPKK (Ste7), and transcription factor (Ste12) to promote either G(1) arrest and fusion or foraging in response ..
  22. Wu C, Leberer E, Thomas D, Whiteway M. Functional characterization of the interaction of Ste50p with Ste11p MAPKKK in Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:2425-40 pubmed
    ..In addition, Ste50p appears to modulate Ste11p autophosphorylation and is itself a substrate of the Ste11p kinase. Therefore, both in vivo and in vitro data support a role for Ste50p in the regulation of Ste11p activity. ..
  23. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
  24. Stevenson B, Rhodes N, Errede B, Sprague G. Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein. Genes Dev. 1992;6:1293-304 pubmed
    ..Each of these suppressor mutations suppresses ste4 and ste5 deletions but not deletions in STE7, STE11, or STE12...
  25. Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher
    ..Taken together, our findings show that budding yeast can access a stable constitutive pseudohyphal growth state with very few genetic and regulatory changes. ..
  26. Breitkreutz A, Boucher L, Tyers M. MAPK specificity in the yeast pheromone response independent of transcriptional activation. Curr Biol. 2001;11:1266-71 pubmed
    ..MAPK specificity in the pheromone response evidently occurs primarily at the substrate level, as opposed to specific kinase activation by dedicated signaling complexes. ..
  27. Yamamoto K, Tatebayashi K, Tanaka K, Saito H. Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor. Mol Cell. 2010;40:87-98 pubmed publisher
    Membrane localization of the Ste11 MAPKKK is essential for activation of both the filamentous growth/invasive growth (FG/IG) MAP kinase (MAPK) pathway and the SHO1 branch of the osmoregulatory HOG MAPK pathway, and is mediated by binding ..
  28. Sheu Y, Santos B, Fortin N, Costigan C, Snyder M. Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis. Mol Cell Biol. 1998;18:4053-69 pubmed
    ..We thus propose that Spa2p, Pea2p, and Bud6p function together, perhaps as a complex, to promote polarized morphogenesis through regulation of the actin cytoskeleton and signaling pathways. ..
  29. Lin M, Unden H, Jacquier N, Schneiter R, Just U, Höfken T. The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell. 2009;20:4826-37 pubmed publisher
    ..Together, these observations suggest that PAKs translocate into the nucleus, where they modulate expression of sterol uptake genes via Sut1, thereby controlling sterol homeostasis. ..
  30. Inouye C, Dhillon N, Thorner J. Ste5 RING-H2 domain: role in Ste4-promoted oligomerization for yeast pheromone signaling. Science. 1997;278:103-6 pubmed
    ..Thus, the RING-H2 domain mediates Ste4-Ste5 interaction, which is a prerequisite for Ste5-Ste5 self-association and signaling. ..
  31. Cherkasova V, Elion E. far4, far5, and far6 define three genes required for efficient activation of MAPKs Fus3 and Kss1 and accumulation of glycogen. Curr Genet. 2001;40:13-26 pubmed
    ..Finally, BIM1 and BIK1 have been identified as CEN suppressors of far5, suggesting that the microtubule apparatus may regulate the ability of the pheromone response pathway to promote G1 arrest. ..
  32. Zuzuarregui A, Kupka T, Bhatt B, Dohnal I, Mudrak I, Friedmann C, et al. M-Track: detecting short-lived protein-protein interactions in vivo. Nat Methods. 2012;9:594-6 pubmed publisher
  33. Barr M, Tu H, Van Aelst L, Wigler M. Identification of Ste4 as a potential regulator of Byr2 in the sexual response pathway of Schizosaccharomyces pombe. Mol Cell Biol. 1996;16:5597-603 pubmed
    ..yeast Schizosaccharomyces pombe, Byr2, a homolog of mammalian MAPK/ERK kinase kinase and Saccharomyces cerevisiae STE11, is required for pheromone-induced sexual differentiation. A screen for S...
  34. Zhou Z, Gartner A, Cade R, Ammerer G, Errede B. Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases. Mol Cell Biol. 1993;13:2069-80 pubmed
    ..Among the components necessary for signal transduction are the STE7 and STE11 kinases and either one of the redundant FUS3 and KSS1 kinases...
  35. Dolan J, Fields S. Overproduction of the yeast STE12 protein leads to constitutive transcriptional induction. Genes Dev. 1990;4:492-502 pubmed
    ..We assayed the effects of overproducing the STE12 protein in both STE+ cells, as well as ste2, ste7, and ste11 mutant cells...
  36. Shively C, Kweon H, Norman K, Mellacheruvu D, Xu T, Sheidy D, et al. Large-Scale Analysis of Kinase Signaling in Yeast Pseudohyphal Development Identifies Regulation of Ribonucleoprotein Granules. PLoS Genet. 2015;11:e1005564 pubmed publisher
    ..Collectively, this study presents a wealth of data identifying the yeast phosphoproteome in pseudohyphal growth and regulatory interrelationships between pseudohyphal growth kinases and RNPs. ..
  37. Niu W, Li Z, Zhan W, Iyer V, Marcotte E. Mechanisms of cell cycle control revealed by a systematic and quantitative overexpression screen in S. cerevisiae. PLoS Genet. 2008;4:e1000120 pubmed publisher
    ..This work thus implicates new genes in cell cycle progression, complements previous screens, and lays the foundation for future experiments to define more precisely roles for these genes in cell cycle progression. ..
  38. Bhunia A, Domadia P, Xu X, Gingras R, Ni F, Bhattacharjya S. Equilibrium unfolding of the dimeric SAM domain of MAPKKK Ste11 from the budding yeast: role of the interfacial residues in structural stability and binding. Biochemistry. 2008;47:651-9 pubmed
    ..The Ste11 protein kinase in the mitogen-activated protein kinase (MAPK) signaling cascades of the budding yeast is regulated ..
  39. Tatebayashi K, Takekawa M, Saito H. A docking site determining specificity of Pbs2 MAPKK for Ssk2/Ssk22 MAPKKKs in the yeast HOG pathway. EMBO J. 2003;22:3624-34 pubmed
    ..Thus, the docking site contributes to both the efficiency and specificity of signaling. During these analyses, we also found a nuclear export signal and a possible nuclear localization signal in Pbs2. ..
  40. Lee P, Shabbir A, Cardozo C, Caplan A. Sti1 and Cdc37 can stabilize Hsp90 in chaperone complexes with a protein kinase. Mol Biol Cell. 2004;15:1785-92 pubmed
    ..We assayed the role of nine different cochaperones in the activation of Ste11, a Saccharomyces cerevisiae mitogen-activated protein kinase kinase kinase...
  41. Slaughter B, Unruh J, Li R. Fluorescence fluctuation spectroscopy and imaging methods for examination of dynamic protein interactions in yeast. Methods Mol Biol. 2011;759:283-306 pubmed publisher
    ..In contrast, live cell fluorescence studies such as those outlined below are able to provide quantitative information on the strength, nature, timing, and location of homotypic and heterotypic protein interactions. ..
  42. Schrick K, Garvik B, Hartwell L. Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone. Genetics. 1997;147:19-32 pubmed
    ..Cells mutant for components of the mitogen-activated protein (MAP) kinase cascade (ste5, ste20, ste11, ste7 or fus3 kss1) formed diploids at a frequency 1% that of the wild-type control, but formed prezygotes as ..
  43. Choi Y, Kim S, Park K, Choi K. Differential transmission of G1 cell cycle arrest and mating signals by Saccharomyces cerevisiae Ste5 mutants in the pheromone pathway. Biochem Cell Biol. 1999;77:459-68 pubmed
    ..cerevisiae. In addition, the roles of Asp-248 and Tyr-421, which are important for pheromone signal transduction were further characterized by site-directed mutagenesis studies. ..
  44. Cullen P, Schultz J, Horecka J, Stevenson B, Jigami Y, Sprague G. Defects in protein glycosylation cause SHO1-dependent activation of a STE12 signaling pathway in yeast. Genetics. 2000;155:1005-18 pubmed
    ..We specifically suggest that a Sho1 --> Ste20/Ste50 --> Ste11 --> Ste7 --> Kss1 --> Ste12 pathway is responsible for activation of FUS1 transcription in these mutants...
  45. Abbas Terki T, Donze O, Picard D. The molecular chaperone Cdc37 is required for Ste11 function and pheromone-induced cell cycle arrest. FEBS Lett. 2000;467:111-6 pubmed
    ..We demonstrate here that Cdc37 is required for activity of the kinase Ste11 in budding yeast...
  46. Lockshon D, Olsen C, Brett C, Chertov A, Merz A, Lorenz D, et al. Rho signaling participates in membrane fluidity homeostasis. PLoS ONE. 2012;7:e45049 pubmed publisher
    ..This work provides the first evidence for the existence of a signaling pathway that enables eukaryotic cells to control membrane fluidity, a requirement for division, differentiation and environmental adaptation. ..
  47. Tatebayashi K, Yamamoto K, Nagoya M, Takayama T, Nishimura A, Sakurai M, et al. Osmosensing and scaffolding functions of the oligomeric four-transmembrane domain osmosensor Sho1. Nat Commun. 2015;6:6975 pubmed publisher
    ..Our results illuminate how the four TM domains of Sho1 dictate the oligomer structure as well as its osmosensing and scaffolding functions. ..
  48. Thorsen M, Di Y, Tängemo C, Morillas M, Ahmadpour D, Van der Does C, et al. The MAPK Hog1p modulates Fps1p-dependent arsenite uptake and tolerance in yeast. Mol Biol Cell. 2006;17:4400-10 pubmed
    ..Understanding how arsenite/antimonite uptake and toxicity is modulated may prove of value for their use in medical therapy. ..
  49. Ahn S, Acurio A, Kron S. Regulation of G2/M progression by the STE mitogen-activated protein kinase pathway in budding yeast filamentous growth. Mol Biol Cell. 1999;10:3301-16 pubmed
    ..We infer that the MAPK pathway promotes filamentous growth by a novel mechanism that inhibits mitotic cyclin/CDK complexes and thereby modulates cell shape, budding pattern, and cell-cell connections. ..
  50. Nakayama N, Kaziro Y, Arai K, Matsumoto K. Role of STE genes in the mating factor signaling pathway mediated by GPA1 in Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:3777-83 pubmed
    The ste mutants (ste2, ste4, ste5, ste7, ste11, and ste12) are insensitive to mating factors and are, therefore, sterile...
  51. Ferrigno P, Posas F, Koepp D, Saito H, Silver P. Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin beta homologs NMD5 and XPO1. EMBO J. 1998;17:5606-14 pubmed
    ..the HOG1 MAP kinase cascade, which is composed of three tiers of protein kinases, namely the SSK2, SSK22 and STE11 MAPKKKs, the PBS2 MAPKK, and the HOG1 MAPK...
  52. Caponigro G, Abedi M, Hurlburt A, Maxfield A, Judd W, Kamb A. Transdominant genetic analysis of a growth control pathway. Proc Natl Acad Sci U S A. 1998;95:7508-13 pubmed
    ..V., Kazarov, A. R., Thimmapaya, R., Axenovich, S. A., Mazo, I. A. & Roninson, I. B. (1994) Proc. Natl. Acad. Sci. USA 91, 3744-3748], suggest that transdominant genetic analysis of the type described here will be broadly applicable. ..
  53. Wojda I, Alonso Monge R, Bebelman J, Mager W, Siderius M. Response to high osmotic conditions and elevated temperature in Saccharomyces cerevisiae is controlled by intracellular glycerol and involves coordinate activity of MAP kinase pathways. Microbiology. 2003;149:1193-204 pubmed
    ..The data taken together indicate the intricate interdependence of growth temperature, intracellular glycerol, cell wall structure and MAP kinase signalling in the hyperosmotic stress response of yeast. ..
  54. Feng Y, Song L, Kincaid E, Mahanty S, Elion E. Functional binding between Gbeta and the LIM domain of Ste5 is required to activate the MEKK Ste11. Curr Biol. 1998;8:267-78 pubmed
    ..through the PAK kinase Ste20 to activate a mitogen-activated protein (MAP) kinase cascade comprising the MEKK Ste11, the MEK Ste7 and two MAP kinases, Fus3 and Kss1...
  55. Adhikari H, Cullen P. Metabolic respiration induces AMPK- and Ire1p-dependent activation of the p38-Type HOG MAPK pathway. PLoS Genet. 2014;10:e1004734 pubmed publisher
    ..Thus, an evolutionarily conserved regulatory axis links metabolic respiration and AMPK to Ire1p, which regulates a differentiation response involving the modulated activity of ERK and p38 MAPK pathways. ..
  56. Basu S, Vadaie N, Prabhakar A, Li B, Adhikari H, Pitoniak A, et al. Spatial landmarks regulate a Cdc42-dependent MAPK pathway to control differentiation and the response to positional compromise. Proc Natl Acad Sci U S A. 2016;113:E2019-28 pubmed publisher
    ..Therefore, a surveillance mechanism monitors spatial position in response to extrinsic and intrinsic stress and modulates the response through a differentiation MAPK pathway. ..
  57. Lyons D, Mahanty S, Choi K, Manandhar M, Elion E. The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:4095-106 pubmed
    ..MAPK) activity and associates with Ste5, the tethering protein essential for activation of the MAPK kinase kinase Ste11. Bem1-Ste5 complexes also contain Ste11, Ste7 (MAPK kinase), and Fus3, suggesting that Ste5 localizes the MAPK ..
  58. Wassmann K, Ammerer G. Overexpression of the G1-cyclin gene CLN2 represses the mating pathway in Saccharomyces cerevisiae at the level of the MEKK Ste11. J Biol Chem. 1997;272:13180-8 pubmed
    ..Increased Cln2 levels repress the ability of hyperactive STE11 alleles to induce the pathway...
  59. Roemer T, Vallier L, Sheu Y, Snyder M. The Spa2-related protein, Sph1p, is important for polarized growth in yeast. J Cell Sci. 1998;111 ( Pt 4):479-94 pubmed
    ..Sph1p also interacts weakly with STE11, the MAPKKK known to activate STE7...
  60. Draper E, Dubrovskyi O, Bar E, Stone D. Dse1 may control cross talk between the pheromone and filamentation pathways in yeast. Curr Genet. 2009;55:611-21 pubmed publisher
    ..pheromone-responsive Gbetagamma, and found to bind both to a Gbetagamma-affinity column, and to the shared MEKK, Ste11. Although overexpression of Dse1 stimulated invasive growth and transcription of both filamentation and mating-..
  61. Reiser V, Salah S, Ammerer G. Polarized localization of yeast Pbs2 depends on osmostress, the membrane protein Sho1 and Cdc42. Nat Cell Biol. 2000;2:620-7 pubmed
    ..Sho1 itself accumulates at sites of polar growth, but independently of stress conditions and Cdc42. These observations allow us to define the sequence of events that occurs during propogation of osmostress signals. ..
  62. Sette C, Inouye C, Stroschein S, Iaquinta P, Thorner J. Mutational analysis suggests that activation of the yeast pheromone response mitogen-activated protein kinase pathway involves conformational changes in the Ste5 scaffold protein. Mol Biol Cell. 2000;11:4033-49 pubmed
    Ste5 is essential for pheromone response and binds components of a mitogen-activated protein kinase (MAPK) cascade: Ste11 (MEKK), Ste7 (MEK), and Fus3 (MAPK)...
  63. Louvion J, Abbas Terki T, Picard D. Hsp90 is required for pheromone signaling in yeast. Mol Biol Cell. 1998;9:3071-83 pubmed
    ..b>Ste11, a yeast equivalent of Raf, forms complexes with wild-type Hsp90 and depends on Hsp90 function for accumulation...
  64. Mösch H, Kübler E, Krappmann S, Fink G, Braus G. Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1325-35 pubmed
  65. Yerko V, Sulea T, Ekiel I, Harcus D, Baardsnes J, Cygler M, et al. Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling. Mol Biol Cell. 2013;24:409-20 pubmed publisher
    ..Although it is known that the MEK kinase of the pathway, Ste11, associates with Ste5, details of this interaction have not been established...
  66. Sotelo J, Rodríguez Gabriel M. Mitogen-activated protein kinase Hog1 is essential for the response to arsenite in Saccharomyces cerevisiae. Eukaryot Cell. 2006;5:1826-30 pubmed
    ..Hog1 is rapidly phosphorylated in response to arsenite and triggers a transcriptional response that involves the upregulation of genes essential for arsenite detoxification. ..
  67. Jansen G, Buhring F, Hollenberg C, Ramezani Rad M. Mutations in the SAM domain of STE50 differentially influence the MAPK-mediated pathways for mating, filamentous growth and osmotolerance in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;265:102-17 pubmed
    ..Thus the Ste50p-Ste11p interaction may differentially modulate the flow of information through the various MAPK-mediated pathways. ..
  68. Park S, Zarrinpar A, Lim W. Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms. Science. 2003;299:1061-4 pubmed
    ..These findings demonstrate that scaffolds are highly flexible organizing factors that can facilitate pathway evolution and engineering. ..
  69. Flom G, Lemieszek M, Fortunato E, Johnson J. Farnesylation of Ydj1 is required for in vivo interaction with Hsp90 client proteins. Mol Biol Cell. 2008;19:5249-58 pubmed publisher
    ..We analyzed the in vivo interaction of Ydj1 with the protein kinase Ste11 and the glucocorticoid receptor...
  70. Mahanty S, Wang Y, Farley F, Elion E. Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade. Cell. 1999;98:501-12 pubmed
    ..This novel regulatory scheme may ensure that cytoplasmic Ste5 does not activate downstream kinases in the absence of pheromone and could be applicable to other membrane-recruited signaling proteins. ..