Gene Symbol: SSZ1
Description: Ssz1p
Alias: PDR13, Ssz1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Gautschi M, Mun A, Ross S, Rospert S. A functional chaperone triad on the yeast ribosome. Proc Natl Acad Sci U S A. 2002;99:4209-14 pubmed
    The chaperones RAC (ribosome-associated complex), consisting of Ssz1p and zuotin, and Ssb1/2p are associated with ribosomes of yeast...
  2. Muldoon Jacobs K, Dinman J. Specific effects of ribosome-tethered molecular chaperones on programmed -1 ribosomal frameshifting. Eukaryot Cell. 2006;5:762-70 pubmed
    The ribosome-associated molecular chaperone complexes RAC (Ssz1p/Zuo1p) and Ssb1p/Ssb2p expose a link between protein folding and translation...
  3. Albanèse V, Yam A, Baughman J, Parnot C, Frydman J. Systems analyses reveal two chaperone networks with distinct functions in eukaryotic cells. Cell. 2006;124:75-88 pubmed
    ..The emergence of a translation-linked chaperone network likely underlies the elaborate cotranslational folding process necessary for the evolution of larger multidomain proteins characteristic of eukaryotic cells. ..
  4. Huang P, Gautschi M, Walter W, Rospert S, Craig E. The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1. Nat Struct Mol Biol. 2005;12:497-504 pubmed
    ..However, Zuo1 efficiently stimulates the ATPase activity of Ssb only when in complex with another Hsp70, Ssz1. Ssz1 binds ATP, but none of the 11 different amino acid substitutions in the ATP-binding cleft affected Ssz1 ..
  5. Gautschi M, Lilie H, Funfschilling U, Mun A, Ross S, Lithgow T, et al. RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin. Proc Natl Acad Sci U S A. 2001;98:3762-7 pubmed
    ..Zuotin is a DnaJ homolog bound to the yeast ribosome. We have now identified the DnaK homolog Ssz1p/Pdr13p as zuotin's partner chaperone...
  6. Weyer F, Gumiero A, Gesé G, Lapouge K, Sinning I. Structural insights into a unique Hsp70-Hsp40 interaction in the eukaryotic ribosome-associated complex. Nat Struct Mol Biol. 2017;24:144-151 pubmed publisher
    ..The ribosome-associated complex (RAC) is unique in that the Hsp40 protein Zuo1 and the atypical Hsp70 chaperone Ssz1 form a stable heterodimer, which acts as a cochaperone for the Hsp70 chaperone Ssb...
  7. Prunuske A, Waltner J, Kuhn P, Gu B, Craig E. Role for the molecular chaperones Zuo1 and Ssz1 in quorum sensing via activation of the transcription factor Pdr1. Proc Natl Acad Sci U S A. 2012;109:472-7 pubmed publisher
    ..In addition, the C terminus of Zuo1 and the N terminus of Ssz1, with which Zuo1 forms a heterodimer, can independently activate the Saccharomyces cerevisiae transcription factor ..
  8. Bosis E, Salomon D, Ohayon O, Sivan G, Bar Nun S, Rabinovich E. Ssz1 restores endoplasmic reticulum-associated protein degradation in cells expressing defective cdc48-ufd1-npl4 complex by upregulating cdc48. Genetics. 2010;184:695-706 pubmed publisher
    ..b>Ssz1p activates Pdr1p, the PDR master regulator...
  9. Michimoto T, Aoki T, Toh e A, Kikuchi Y. Yeast Pdr13p and Zuo1p molecular chaperones are new functional Hsp70 and Hsp40 partners. Gene. 2000;257:131-7 pubmed
    ..ZUO1 encodes a DnaJ-related Hsp40. Here we show that a recessive cold sensitive mutation in PDR13 coding for an Hsp70 suppressed the tom1 mutation...

More Information


  1. Hallstrom T, Moye Rowley W. Hyperactive forms of the Pdr1p transcription factor fail to respond to positive regulation by the hsp70 protein Pdr13p. Mol Microbiol. 2000;36:402-13 pubmed
    ..Previous experiments have demonstrated that the Hsp70 protein encoded by the PDR13 gene is a positive regulator of Pdr1p function...
  2. Rakwalska M, Rospert S. The ribosome-bound chaperones RAC and Ssb1/2p are required for accurate translation in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:9186-97 pubmed
    ..Based on the combined data we conclude that RAC and Ssb1/2p are crucial in maintaining translational fidelity beyond their postulated role as chaperones for nascent polypeptides. ..
  3. Conz C, Otto H, Peisker K, Gautschi M, Wölfle T, Mayer M, et al. Functional characterization of the atypical Hsp70 subunit of yeast ribosome-associated complex. J Biol Chem. 2007;282:33977-84 pubmed
    ..a stable chaperone complex termed ribosome-associated complex consisting of the J-domain protein Zuo1 and the Hsp70 Ssz1. Zuo1 and Ssz1 together with the Hsp70 homolog Ssb1/2 form a functional triad involved in translation and early ..
  4. Zhang Y, Ma C, Yuan Y, Zhu J, Li N, Chen C, et al. Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome. Nat Struct Mol Biol. 2014;21:1042-6 pubmed publisher
  5. Hallstrom T, Katzmann D, Torres R, Sharp W, Moye Rowley W. Regulation of transcription factor Pdr1p function by an Hsp70 protein in Saccharomyces cerevisiae. Mol Cell Biol. 1998;18:1147-55 pubmed
    ..This gene has been designated PDR13 and is required for normal growth...
  6. Dechamps C, Portetelle D, Vandenbol M. Multicopy suppression screen in the msb3 msb4 Saccharomyces cerevisiae double mutant, affected in Ypt/RabGAP activity. Biotechnol Lett. 2005;27:1439-49 pubmed
    ..of functional features into four groups: vesicular transport proteins (Sec7p, Vps35p, and Uso1p), a protein involved in cell division (Sap155p), a molecular chaperon (Ssz1p), and a protein associated with the 25S proteasome (Cic1p).
  7. Peisker K, Braun D, Wölfle T, Hentschel J, Funfschilling U, Fischer G, et al. Ribosome-associated complex binds to ribosomes in close proximity of Rpl31 at the exit of the polypeptide tunnel in yeast. Mol Biol Cell. 2008;19:5279-88 pubmed publisher
    Ribosome-associated complex (RAC) consists of the Hsp40 homolog Zuo1 and the Hsp70 homolog Ssz1. The chaperone participates in the biogenesis of newly synthesized polypeptides...
  8. Albanèse V, Reissmann S, Frydman J. A ribosome-anchored chaperone network that facilitates eukaryotic ribosome biogenesis. J Cell Biol. 2010;189:69-81 pubmed publisher
    ..Our results demonstrate that, in addition to their known cytoplasmic roles in de novo protein folding, some ribosome-anchored CLIPS chaperones play a critical role in nuclear steps of ribosome biogenesis. ..
  9. Leidig C, Bange G, Kopp J, Amlacher S, Aravind A, Wickles S, et al. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Nat Struct Mol Biol. 2013;20:23-8 pubmed publisher
    ..RAC) from Saccharomyces cerevisiae and Chaetomium thermophilum, consisting of heat-shock protein 70 (Hsp70) Ssz1 and the Hsp40 Zuo1...
  10. Hundley H, Eisenman H, Walter W, Evans T, Hotokezaka Y, Wiedmann M, et al. The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain. Proc Natl Acad Sci U S A. 2002;99:4203-8 pubmed
    Two proteins of the Hsp70 class (Ssb and Ssz1) and one of the J-type class (Zuo1) of molecular chaperones reside on the yeast ribosome, with Ssz1 forming a stable heterodimer with Zuo1...
  11. Tomala K, Korona R. Alleviation of deleterious effects of protein mutation through inactivation of molecular chaperones. Mol Genet Genomics. 2008;280:409-17 pubmed publisher
    ..We suggest that under non-stressful conditions, molecular chaperones are tuned to support folding of native proteins, but not that of mutated ones. ..
  12. Fiaux J, Horst J, Scior A, Preissler S, Koplin A, Bukau B, et al. Structural analysis of the ribosome-associated complex (RAC) reveals an unusual Hsp70/Hsp40 interaction. J Biol Chem. 2010;285:3227-34 pubmed publisher
    ..Taken together, we present a novel mechanism for converting Zuotin and Ssz chaperones into a functionally active dimer. ..
  13. Desany B, Alcasabas A, Bachant J, Elledge S. Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway. Genes Dev. 1998;12:2956-70 pubmed
    ..We propose that this checkpoint pathway plays an important role in the maintenance of DNA synthetic capabilities when DNA replication is stressed. ..