Gene Symbol: SSY5
Description: Ssy5p
Alias: RAA3, Ssy5p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Andréasson C, Heessen S, Ljungdahl P. Regulation of transcription factor latency by receptor-activated proteolysis. Genes Dev. 2006;20:1563-8 pubmed endoproteolytically processed in response to extracellular amino acids by the plasma membrane SPS (Ssy1-Ptr3-Ssy5)-sensor...
  2. Poulsen P, Lo Leggio L, Kielland Brandt M. Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell. 2006;5:601-8 pubmed
    b>Ssy5p is a 77-kDa protein believed to be a component of the SPS amino acid sensor complex in the plasma membrane of Saccharomyces cerevisiae...
  3. Liu Z, Thornton J, Spirek M, Butow R. Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3. Mol Cell Biol. 2008;28:551-63 pubmed
    ..pathway, which consists of Ssy1, an amino acid sensor on the plasma membrane, and two downstream factors, Ptr3 and Ssy5. Upon activation of SPS signaling, two transcription factors, Stp1 and Stp2, undergo Ssy5-dependent proteolytic ..
  4. Tumusiime S, Zhang C, Overstreet M, Liu Z. Differential regulation of transcription factors Stp1 and Stp2 in the Ssy1-Ptr3-Ssy5 amino acid sensing pathway. J Biol Chem. 2011;286:4620-31 pubmed publisher
    ..Our results indicate that the functions of two seemingly redundant transcription factors can be separated by differential degradation and distinct cellular localization. ..
  5. Forsberg H, Ljungdahl P. Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol. 2001;21:814-26 pubmed
    ..Although Ssy5p is predicted to be a soluble protein, it exhibits properties indicating that it is a peripherally associated ..
  6. Abdel Sater F, El Bakkoury M, Urrestarazu A, Vissers S, Andre B. Amino acid signaling in yeast: casein kinase I and the Ssy5 endoprotease are key determinants of endoproteolytic activation of the membrane-bound Stp1 transcription factor. Mol Cell Biol. 2004;24:9771-85 pubmed
    ..This sensing function requires the permease-like Ssy1 and associated Ptr3 and Ssy5 proteins, all essential to activation, by endoproteolytic processing, of the membrane-bound Stp1 transcription ..
  7. Omnus D, Pfirrmann T, Andréasson C, Ljungdahl P. A phosphodegron controls nutrient-induced proteasomal activation of the signaling protease Ssy5. Mol Biol Cell. 2011;22:2754-65 pubmed publisher
    Regulated proteolysis serves as a mechanism to control cellular processes. The SPS (Ssy1-Ptr3-Ssy5) sensor in yeast responds to extracellular amino acids by endoproteolytically activating transcription factors Stp1 and Stp2 (Stp1/2)...
  8. Bernard F, Andre B. Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae. Mol Microbiol. 2001;41:489-502 pubmed
    ..Data obtained by overproducing Ptr3p and Ssy5p in ssy1Delta, ptr3Delta and ssy5Delta mutants suggest that Ptr3p acts downstream from Ssy1p and Ssy5p downstream ..
  9. Pfirrmann T, Heessen S, Omnus D, Andréasson C, Ljungdahl P. The prodomain of Ssy5 protease controls receptor-activated proteolysis of transcription factor Stp1. Mol Cell Biol. 2010;30:3299-309 pubmed publisher
    ..b>Ssy5, the activating endoprotease, is synthesized with a large N-terminal prodomain and a C-terminal chymotrypsin-like ..

More Information


  1. Abdel Sater F, Jean C, Merhi A, Vissers S, Andre B. Amino acid signaling in yeast: activation of Ssy5 protease is associated with its phosphorylation-induced ubiquitylation. J Biol Chem. 2011;286:12006-15 pubmed publisher
    The yeast Ssy5 protein is a serine-type endoprotease autoprocessed into a catalytic domain and a large inhibitory prodomain...
  2. Menant A, Barbey R, Thomas D. Substrate-mediated remodeling of methionine transport by multiple ubiquitin-dependent mechanisms in yeast cells. EMBO J. 2006;25:4436-47 pubmed
    ..The multiple ubiquitin-dependent mechanisms that function in methionine transport regulation further exemplify the pervasive role of ubiquitin in the adaptation of single-cell organisms to environmental modifications. ..
  3. Omnus D, Ljungdahl P. Rts1-protein phosphatase 2A antagonizes Ptr3-mediated activation of the signaling protease Ssy5 by casein kinase I. Mol Biol Cell. 2013;24:1480-92 pubmed publisher
    ..In response to extracellular amino acids, the yeast Ssy1-Ptr3-Ssy5 sensor triggers the endoproteolytic processing of transcription factors Stp1 and Stp2 to induce amino acid uptake...
  4. Tsang F, James C, Kato M, Myers V, Ilyas I, Tsang M, et al. Reduced Ssy1-Ptr3-Ssy5 (SPS) signaling extends replicative life span by enhancing NAD+ homeostasis in Saccharomyces cerevisiae. J Biol Chem. 2015;290:12753-64 pubmed publisher
    ..Here we identify the Ssy1-Ptr3-Ssy5 (SPS) amino acid sensing pathway as a novel longevity factor...