SST2

Summary

Gene Symbol: SST2
Description: GTPase-activating protein SST2
Alias: GTPase-activating protein SST2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Apanovitch D, Slep K, Sigler P, Dohlman H. Sst2 is a GTPase-activating protein for Gpa1: purification and characterization of a cognate RGS-Galpha protein pair in yeast. Biochemistry. 1998;37:4815-22 pubmed
    Genetic studies in the yeast Saccharomyces cerevisiae have shown that SST2 promotes pheromone desensitization in vivo...
  2. Konopka J, Jenness D. Genetic fine-structural analysis of the Saccharomyces cerevisiae alpha-pheromone receptor. Cell Regul. 1991;2:439-52 pubmed
    ..Increased gene dosage of these N-terminal alleles suppressed their mutant phenotypes, whereas the sst2-1 mutation, which blocks adaptation to pheromone, did not result in suppression...
  3. Leavitt L, Macaluso C, Kim K, Martin N, Dumont M. Dominant negative mutations in the alpha-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae. Mol Gen Genet. 1999;261:917-32 pubmed
    ..The dominant negative behavior of the mutant receptors is diminished by deletion of the SST2 gene, which encodes an RGS (Regulator of G protein Signaling) protein involved in desensitization of pheromone ..
  4. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
  5. Parnell S, Marotti L, Kiang L, Torres M, Borchers C, Dohlman H. Phosphorylation of the RGS protein Sst2 by the MAP kinase Fus3 and use of Sst2 as a model to analyze determinants of substrate sequence specificity. Biochemistry. 2005;44:8159-66 pubmed
    Previously, we used mass spectrometry to demonstrate pheromone-stimulated phosphorylation of Ser-539 in Sst2, a regulator of G protein signaling in yeast Saccharomyces cerevisiae [Garrison, T. R., et al. (1999) J. Biol. Chem...
  6. Chasse S, Flanary P, Parnell S, Hao N, Cha J, Siderovski D, et al. Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae. Eukaryot Cell. 2006;5:330-46 pubmed
    ..In the yeast Saccharomyces cerevisiae there are four RGS-homologous proteins (Sst2, Rgs2, Rax1, and Mdm1) and two Galpha proteins (Gpa1 and Gpa2)...
  7. Blinder D, Jenness D. Regulation of postreceptor signaling in the pheromone response pathway of Saccharomyces cerevisiae. Mol Cell Biol. 1989;9:3720-6 pubmed
    ..After prolonged exposure to alpha-factor, the cells adapt to the stimulus and resume cell division. The sst2 mutation is known to inhibit adaptation...
  8. Dohlman H, Apaniesk D, Chen Y, Song J, Nusskern D. Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:3635-43 pubmed
    ..To test this model directly, we isolated and characterized several dominant gain-of-function mutants of SST2. These mutations block the normal pheromone response, including a loss of pheromone-stimulated gene transcription, ..
  9. Alvaro C, O Donnell A, Prosser D, Augustine A, Goldman A, Brodsky J, et al. Specific ?-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2. Mol Cell Biol. 2014;34:2660-81 pubmed
    ..of the expression of an ?-factor-degrading protease (Bar1), a regulator of G-protein signaling protein (Sst2) that stimulates Gpa1-GTP hydrolysis, and Gpa1 itself...

More Information

Publications30

  1. Rivers D, Sprague G. Autocrine activation of the pheromone response pathway in matalpha2- cells is attenuated by SST2- and ASG7-dependent mechanisms. Mol Genet Genomics. 2003;270:225-33 pubmed
    ..We identified two genes, SST2 and ASG7...
  2. DiBello P, Garrison T, Apanovitch D, Hoffman G, Shuey D, Mason K, et al. Selective uncoupling of RGS action by a single point mutation in the G protein alpha-subunit. J Biol Chem. 1998;273:5780-4 pubmed
    ..describe here a Galpha mutant in yeast (gpa1(sst)) that phenotypically mimics the loss of its cognate RGS protein (SST2)...
  3. Maytal Kivity V, Piran R, Pick E, Hofmann K, Glickman M. COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae. EMBO Rep. 2002;3:1215-21 pubmed
    ..Overexpression of individual subunits or a human homolog mitigates sst2-induced pheromone sensitivity. Csi1, a novel CSN interactor, exhibits opposite phenotypes...
  4. Dohlman H, Song J, Ma D, Courchesne W, Thorner J. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). Mol Cell Biol. 1996;16:5194-209 pubmed
    b>Sst2 is the prototype for the newly recognized RGS (for regulators of G-protein signaling) family. Cells lacking the pheromone-inducible SST2 gene product fail to resume growth after exposure to pheromone...
  5. Kallal L, Fishel R. The GTP hydrolysis defect of the Saccharomyces cerevisiae mutant G-protein Gpa1(G50V). Yeast. 2000;16:387-400 pubmed
    ..These biochemical results can explain many of the known gpa1(G50V) yeast cell phenotypes. ..
  6. Rad M, Xu G, Hollenberg C. STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae. Mol Gen Genet. 1992;236:145-54 pubmed
    ..The ste50-2 mutation partially suppresses the desensitisation defect of the sst2-1 mutation, and the resulting ste50-2 sst2-1 mutants restore fertility...
  7. Courchesne W, Kunisawa R, Thorner J. A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae. Cell. 1989;58:1107-19 pubmed
    MATa cells carrying an sst2 mutation are unable to recover from the G1-specific cell cycle arrest induced by the mating pheromone alpha factor. The KSS1 gene, when overexpressed, suppresses this adaptation defect...
  8. Chen T, Kurjan J. Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest. Mol Cell Biol. 1997;17:3429-39 pubmed
    b>SST2 plays an important role in the sensitivity of yeast cells to pheromone and in recovery from pheromone-induced G1 arrest...
  9. Hasson M, Blinder D, Thorner J, Jenness D. Mutational activation of the STE5 gene product bypasses the requirement for G protein beta and gamma subunits in the yeast pheromone response pathway. Mol Cell Biol. 1994;14:1054-65 pubmed
    ..The slow-growth phenotype manifested by cells carrying STE5Hyp alleles was enhanced by the sst2-1 mutation; this effect was eliminated in ste4 mutants...
  10. Xu B, Skowronek K, Kurjan J. The N terminus of Saccharomyces cerevisiae Sst2p plays an RGS-domain-independent, Mpt5p-dependent role in recovery from pheromone arrest. Genetics. 2001;159:1559-71 pubmed
    ..the GPA1(G302S) mutation, which uncouples Gpa1p from Sst2p, results in pheromone supersensitivity similar to the sst2 mutant, and promotion of recovery by overexpression of Sst2p is dependent on both Mpt5p and the Gpa1p interaction...
  11. Ballon D, Flanary P, Gladue D, Konopka J, Dohlman H, Thorner J. DEP-domain-mediated regulation of GPCR signaling responses. Cell. 2006;126:1079-93 pubmed
    ..We examined the yeast regulator of G protein signaling (RGS) protein Sst2 and demonstrate that the DEP domains in Sst2 mediate binding to its cognate GPCR (Ste2)...
  12. Leberer E, Thomas D, Whiteway M. Pheromone signalling and polarized morphogenesis in yeast. Curr Opin Genet Dev. 1997;7:59-66 pubmed
    ..Experiments suggest that binding of the Rho-like GTPase Cdc42p to Ste20p is not required for the mating response, yet is needed for the pseudohyphal growth response which involves many of the same kinases. ..
  13. Dietzel C, Kurjan J. The yeast SCG1 gene: a G alpha-like protein implicated in the a- and alpha-factor response pathway. Cell. 1987;50:1001-10 pubmed
    We have identified the SCG1 gene by its ability to suppress the pheromone-supersensitive sst2-1 mutation...
  14. Jenness D, Goldman B, Hartwell L. Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression. Mol Cell Biol. 1987;7:1311-9 pubmed
    ..mutant was apparently limited by its sensitivity to alpha-factor, as its sterility was suppressed by mutation sst2-1, which leads to enhanced alpha-factor sensitivity...
  15. Leza M, Elion E. POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2. Genetics. 1999;151:531-43 pubmed
    ..These and other results suggest that POG1 may regulate additional genes during vegetative growth and recovery. ..
  16. Burchett S, Flanary P, Aston C, Jiang L, Young K, Uetz P, et al. Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae. J Biol Chem. 2002;277:22156-67 pubmed
    ..The RGS in yeast, Sst2, can inhibit a G protein signal leading to mating...
  17. Garrison T, Zhang Y, Pausch M, Apanovitch D, Aebersold R, Dohlman H. Feedback phosphorylation of an RGS protein by MAP kinase in yeast. J Biol Chem. 1999;274:36387-91 pubmed
    ..To address this question we purified the RGS in yeast, Sst2, and used electrospray ionization mass spectrometry to identify post-translational modifications...
  18. Dixit G, Kelley J, Houser J, Elston T, Dohlman H. Cellular noise suppression by the regulator of G protein signaling Sst2. Mol Cell. 2014;55:85-96 pubmed publisher
    ..We found that the RGS protein Sst2 limits variability in transcription and morphogenesis in response to pheromone stimulation...
  19. Dietzel C, Kurjan J. Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone. Mol Cell Biol. 1987;7:4169-77 pubmed
    Strains of both haploid mating types containing sst2 mutations are altered in response to pheromone; MATa sst2 cells are supersensitive to alpha-factor, and MAT alpha sst2 cells are supersensitive to a-factor...
  20. Venkatapurapu S, Kelley J, Dixit G, Pena M, Errede B, Dohlman H, et al. Modulation of receptor dynamics by the regulator of G protein signaling Sst2. Mol Biol Cell. 2015;26:4124-34 pubmed publisher
    ..The regulator of G protein signaling (RGS) protein Sst2 acts by accelerating GTP hydrolysis and facilitating pathway desensitization...
  21. Bush A, Vasen G, Constantinou A, Dunayevich P, Patop I, Blaustein M, et al. Yeast GPCR signaling reflects the fraction of occupied receptors, not the number. Mol Syst Biol. 2016;12:898 pubmed publisher
    ..occupancy depends on the physical interaction between the inhibitory regulator of G-protein signaling (RGS), Sst2, and the receptor...