SSO2

Summary

Gene Symbol: SSO2
Description: syntaxin
Alias: syntaxin
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Brennwald P, Kearns B, Champion K, Keranen S, Bankaitis V, Novick P. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 1994;79:245-58 pubmed
    ..Our results identify Sec9 as the yeast cognate of SNAP-25 and suggest that SNARE complexes acting at specific stages of vesicular transport serve as the ultimate targets of regulation by members of the Sec4/Ypt1/Rab family of GTPases. ..
  2. Knop M, Miller K, Mazza M, Feng D, Weber M, Keränen S, et al. Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast. Mol Biol Cell. 2005;16:4543-56 pubmed
    ..These results position Mso1p in the interface of the exocyst complex, Sec4p, and the SNARE machinery, and reveal a novel layer of molecular conservation in the exocytosis machinery. ..
  3. Aalto M, Ronne H, Keranen S. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J. 1993;12:4095-104 pubmed
    ..Also related to Sso1p and Sso2p are three mammalian proteins: epimorphin, syntaxin A/HPC-1 and syntaxin B. A nematode cDNA product also belongs to the new protein family...
  4. Weber M, Chernov K, Turakainen H, Wohlfahrt G, Pajunen M, Savilahti H, et al. Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1. Mol Biol Cell. 2010;21:1362-74 pubmed publisher
    ..In many SM family proteins, domain 1 binds to a N-terminal peptide of a syntaxin family protein. The Sec1p-interacting syntaxins Sso1p and Sso2p lack the N-terminal peptide...
  5. Wiederkehr A, De Craene J, Ferro Novick S, Novick P. Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol. 2004;167:875-87 pubmed
    ..Furthermore, a fraction of Sec1p can be coprecipitated with the exoycst. Our results suggest that Sec1p couples exocyst-mediated vesicle tethering with SNARE-mediated docking and fusion. ..
  6. Brummer M, Kivinen K, Jantti J, Toikkanen J, Soderlund H, Keranen S. Characterization of the sec1-1 and sec1-11 mutations. Yeast. 2001;18:1525-36 pubmed
    ..These results localize sec1-1 and sec1-11 mutations to a domain of Sec1p and suggest a mechanism by which sec1-1 and sec1-11 cells become temperature-sensitive. ..
  7. Carr C, Grote E, Munson M, Hughson F, Novick P. Sec1p binds to SNARE complexes and concentrates at sites of secretion. J Cell Biol. 1999;146:333-44 pubmed
    ..Sec1 family have been shown to interact with target-membrane t-SNAREs that are homologous to the neuronal protein syntaxin. We demonstrate that yeast Sec1p coprecipitates not only the syntaxin homologue Ssop, but also the other two ..
  8. Grote E, Carr C, Novick P. Ordering the final events in yeast exocytosis. J Cell Biol. 2000;151:439-52 pubmed
    ..In the sec18-1 mutant, cis-SNARE complexes containing surface-accessible Sncp accumulate in the plasma membrane. Thus, one function of Sec18p is to disassemble SNARE complexes on the postfusion membrane. ..
  9. Adamo J, Moskow J, Gladfelter A, Viterbo D, Lew D, Brennwald P. Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud. J Cell Biol. 2001;155:581-92 pubmed
    ..Rather, we suggest that Cdc42 acts as an allosteric regulator of the vesicle docking and fusion apparatus to provide maximal function at sites of polarized growth. ..

More Information

Publications25

  1. Jantti J, Aalto M, Oyen M, Sundqvist L, Keränen S, Ronne H. Characterization of temperature-sensitive mutations in the yeast syntaxin 1 homologues Sso1p and Sso2p, and evidence of a distinct function for Sso1p in sporulation. J Cell Sci. 2002;115:409-20 pubmed
    The duplicated genes SSO1 and SSO2 encode yeast homologues of syntaxin 1 and perform an essential function during fusion of secretory vesicles at the plasma membrane...
  2. Aalto M, Jantti J, Ostling J, Keranen S, Ronne H. Mso1p: a yeast protein that functions in secretion and interacts physically and genetically with Sec1p. Proc Natl Acad Sci U S A. 1997;94:7331-6 pubmed
    ..We previously have cloned two yeast genes encoding syntaxins, SSO1 and SSO2, as suppressors of the temperature-sensitive sec1-1 mutation...
  3. Williams D, Novick P. Analysis of SEC9 suppression reveals a relationship of SNARE function to cell physiology. PLoS ONE. 2009;4:e5449 pubmed publisher
    ..Second, Sro7p acts to promote SNARE complex formation. Finally, Sec9p function and SNARE complex formation are tightly coupled to the physiological state of the cell. ..
  4. Grote E, Baba M, Ohsumi Y, Novick P. Geranylgeranylated SNAREs are dominant inhibitors of membrane fusion. J Cell Biol. 2000;151:453-66 pubmed
    ..High-level expression of mutant Snc1 or Sso2 proteins that have a COOH-terminal geranylgeranylation signal instead of a transmembrane domain inhibits exocytosis ..
  5. Qi C, Zhang H, Liu L, Yang R, Yang Y, Kang T, et al. Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array. Sci Rep. 2014;4:5341 pubmed publisher
    ..We argue that the use of imaging ellipsometer coupled with microfluidic device will deepen our understanding of the molecular mechanisms underlying membrane fusion process. ..
  6. Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, et al. The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p. J Cell Biol. 2013;202:509-26 pubmed publisher
    ..We propose that the exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins. This could help to confer both specificity and directionality to vesicular traffic. ..
  7. Nair U, Jotwani A, Geng J, Gammoh N, Richerson D, Yen W, et al. SNARE proteins are required for macroautophagy. Cell. 2011;146:290-302 pubmed publisher
    ..Thus, multiple SNARE-mediated fusion events are likely to be involved in autophagosome biogenesis. ..
  8. Weber Boyvat M, Aro N, Chernov K, Nyman T, Jantti J. Sec1p and Mso1p C-terminal tails cooperate with the SNAREs and Sec4p in polarized exocytosis. Mol Biol Cell. 2011;22:230-44 pubmed publisher
    ..The bimolecular fluorescence complementation (BiFC) assay results suggest that, in the SNARE-deficient sso2-1 ?sso1 cells, Mso1p, a Sec1p binding protein, helps to target Sec1p(1-657) lacking the C-terminal tail to the ..
  9. Mendonsa R, Engebrecht J. Phosphatidylinositol-4,5-bisphosphate and phospholipase D-generated phosphatidic acid specify SNARE-mediated vesicle fusion for prospore membrane formation. Eukaryot Cell. 2009;8:1094-105 pubmed publisher
    ..The SNARE syntaxin Sso1p is essential for PSM production while the functionally redundant homolog in vegetative growth, Sso2p, is ..
  10. Liu S, Wilson K, Rice Stitt T, Neiman A, McNew J. In vitro fusion catalyzed by the sporulation-specific t-SNARE light-chain Spo20p is stimulated by phosphatidic acid. Traffic. 2007;8:1630-43 pubmed
    ..Our results suggest that the fusion strength of SNAREs, the composition of organelle lipids and lipid-SNARE interactions may be coordinately regulated to control the rate and specificity of membrane fusion. ..
  11. Zhang X, Wang P, Gangar A, Zhang J, Brennwald P, TerBush D, et al. Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis. J Cell Biol. 2005;170:273-83 pubmed
    ..We propose that, although Lgl is broadly distributed in the cells, its localized interaction with the exocyst and kinetic activation are important for the establishment and reenforcement of cell polarity. ..
  12. Ossig R, Schmitt H, de Groot B, Riedel D, Keranen S, Ronne H, et al. Exocytosis requires asymmetry in the central layer of the SNARE complex. EMBO J. 2000;19:6000-10 pubmed
    ..We conclude that the asymmetric arrangement of the polar amino acids in the central layer is essential for normal function of SNAREs in membrane fusion. ..
  13. Gerst J. Conserved alpha-helical segments on yeast homologs of the synaptobrevin/VAMP family of v-SNAREs mediate exocytic function. J Biol Chem. 1997;272:16591-8 pubmed
    ..competence to snc cells and to interact genetically with components of the proposed fusion complex: the Sec9 and Sso2 t-SNAREs and the Sec17 alpha-SNAP homolog...
  14. Schindler C, Spang A. Interaction of SNAREs with ArfGAPs precedes recruitment of Sec18p/NSF. Mol Biol Cell. 2007;18:2852-63 pubmed
  15. Forsmark A, Rossi G, Wadskog I, Brennwald P, Warringer J, Adler L. Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion. Traffic. 2011;12:740-53 pubmed publisher
  16. Pelham H. SNAREs and the secretory pathway-lessons from yeast. Exp Cell Res. 1999;247:1-8 pubmed
    ..This review summarizes current knowledge of the yeast SNAREs and the picture of the secretory pathway that emerges from such studies. ..